Aquisalimonas asiatica
Average proteome isoelectric point is 5.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3698 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H8SZY3|A0A1H8SZY3_9GAMM ADP-ribosylglycohydrolase OS=Aquisalimonas asiatica OX=406100 GN=SAMN04488052_103324 PE=4 SV=1
MM1 pKa = 6.49 TTRR4 pKa = 11.84 TSATTVFCIACASLLNGCFGGSSSGSGAGDD34 pKa = 4.1 DD35 pKa = 3.89 NDD37 pKa = 3.98 GVQPEE42 pKa = 4.34 DD43 pKa = 3.48 VQVRR47 pKa = 11.84 QFDD50 pKa = 3.82 ASDD53 pKa = 3.37 HH54 pKa = 6.85 DD55 pKa = 4.4 EE56 pKa = 4.37 PVHH59 pKa = 7.08 VDD61 pKa = 3.83 LSTGEE66 pKa = 4.27 VLTLSAGEE74 pKa = 4.28 AADD77 pKa = 4.09 SEE79 pKa = 4.47 AWDD82 pKa = 3.38 IALQRR87 pKa = 11.84 HH88 pKa = 5.28 QISLNGGTSGPGRR101 pKa = 11.84 VQGALLDD108 pKa = 4.35 GQASFYY114 pKa = 11.46 NEE116 pKa = 4.84 DD117 pKa = 3.46 GTPNASVFTNTGAADD132 pKa = 3.64 TLDD135 pKa = 4.08 ALLAPVDD142 pKa = 4.23 AVDD145 pKa = 4.01 SGDD148 pKa = 3.42 WVLDD152 pKa = 3.92 SITTEE157 pKa = 5.38 FGDD160 pKa = 3.26 DD161 pKa = 2.86 WYY163 pKa = 10.14 IYY165 pKa = 11.03 DD166 pKa = 4.01 FATGEE171 pKa = 4.1 FQPDD175 pKa = 3.9 PDD177 pKa = 4.27 NGWLLRR183 pKa = 11.84 SGTGDD188 pKa = 2.56 SYY190 pKa = 11.81 ARR192 pKa = 11.84 VRR194 pKa = 11.84 ITEE197 pKa = 4.09 LDD199 pKa = 3.37 FDD201 pKa = 4.15 SRR203 pKa = 11.84 AGDD206 pKa = 3.6 GVEE209 pKa = 3.83 HH210 pKa = 6.59 FRR212 pKa = 11.84 FEE214 pKa = 4.75 FDD216 pKa = 3.37 VQVPNTQQFTEE227 pKa = 4.36 TAVFTGSLPGGGGDD241 pKa = 3.68 LCFDD245 pKa = 3.84 VDD247 pKa = 4.03 SDD249 pKa = 3.87 ATADD253 pKa = 3.72 CSGVDD258 pKa = 3.22 WDD260 pKa = 4.26 IQIGFQGQDD269 pKa = 2.75 FYY271 pKa = 11.83 LRR273 pKa = 11.84 SNSGVSGDD281 pKa = 3.36 GDD283 pKa = 3.47 GGVFGPFPWSEE294 pKa = 3.9 LEE296 pKa = 4.58 AYY298 pKa = 10.23 DD299 pKa = 4.2 SATVTPSGEE308 pKa = 4.43 SIAGHH313 pKa = 5.34 YY314 pKa = 10.01 QPDD317 pKa = 3.76 ASTGIFDD324 pKa = 4.23 AYY326 pKa = 10.58 SWYY329 pKa = 10.54 AYY331 pKa = 10.87 NLTGQHH337 pKa = 5.6 QLWPNYY343 pKa = 8.99 RR344 pKa = 11.84 VYY346 pKa = 11.03 LIDD349 pKa = 4.35 SDD351 pKa = 4.24 HH352 pKa = 7.44 DD353 pKa = 3.73 EE354 pKa = 4.7 DD355 pKa = 5.52 AAAQYY360 pKa = 11.08 ALQVINYY367 pKa = 8.91 YY368 pKa = 10.83 DD369 pKa = 3.48 GTGDD373 pKa = 3.37 SGHH376 pKa = 7.27 PEE378 pKa = 4.0 LRR380 pKa = 11.84 WRR382 pKa = 11.84 QVDD385 pKa = 3.22
Molecular weight: 41.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.617
IPC_protein 3.668
Toseland 3.427
ProMoST 3.821
Dawson 3.681
Bjellqvist 3.846
Wikipedia 3.63
Rodwell 3.49
Grimsley 3.338
Solomon 3.668
Lehninger 3.63
Nozaki 3.783
DTASelect 4.075
Thurlkill 3.49
EMBOSS 3.643
Sillero 3.795
Patrickios 0.566
IPC_peptide 3.668
IPC2_peptide 3.77
IPC2.peptide.svr19 3.729
Protein with the highest isoelectric point:
>tr|A0A1H8VD67|A0A1H8VD67_9GAMM Uncharacterized protein OS=Aquisalimonas asiatica OX=406100 GN=SAMN04488052_11139 PE=4 SV=1
MM1 pKa = 7.12 FRR3 pKa = 11.84 MFRR6 pKa = 11.84 MFRR9 pKa = 11.84 MFRR12 pKa = 11.84 MFRR15 pKa = 11.84 MFRR18 pKa = 11.84 MFRR21 pKa = 11.84 MFRR24 pKa = 11.84 MFRR27 pKa = 11.84 MFRR30 pKa = 11.84 VVRR33 pKa = 11.84 VVRR36 pKa = 11.84 VVRR39 pKa = 11.84 VVRR42 pKa = 11.84 VVRR45 pKa = 11.84 VVRR48 pKa = 3.93
Molecular weight: 6.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.52
IPC2_protein 11.403
IPC_protein 13.013
Toseland 13.173
ProMoST 13.685
Dawson 13.173
Bjellqvist 13.173
Wikipedia 13.656
Rodwell 12.676
Grimsley 13.217
Solomon 13.671
Lehninger 13.583
Nozaki 13.173
DTASelect 13.173
Thurlkill 13.173
EMBOSS 13.671
Sillero 13.173
Patrickios 12.398
IPC_peptide 13.685
IPC2_peptide 12.676
IPC2.peptide.svr19 9.326
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3698
0
3698
1223554
40
6102
330.9
36.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.413 ± 0.056
0.854 ± 0.012
6.564 ± 0.045
6.473 ± 0.039
3.373 ± 0.028
8.741 ± 0.041
2.411 ± 0.021
4.332 ± 0.031
1.977 ± 0.03
10.519 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.491 ± 0.02
2.469 ± 0.025
5.036 ± 0.032
3.616 ± 0.025
7.969 ± 0.043
4.961 ± 0.028
5.184 ± 0.027
7.783 ± 0.035
1.453 ± 0.018
2.38 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here