Cryphonectria parasitica bipartite mycovirus 1

Taxonomy: Viruses; Riboviria; dsRNA viruses; unclassified dsRNA viruses

Average proteome isoelectric point is 7.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4JRU3|R4JRU3_9VIRU Putative RNA-dependent RNA polymerase OS=Cryphonectria parasitica bipartite mycovirus 1 OX=1329781 PE=4 SV=1
MM1 pKa = 7.77EE2 pKa = 5.04EE3 pKa = 3.43MDD5 pKa = 3.9AATFNLQPIRR15 pKa = 11.84SITDD19 pKa = 4.05TIQRR23 pKa = 11.84QWLRR27 pKa = 11.84QSPSAEE33 pKa = 3.71PMVIDD38 pKa = 4.25PQTATTAADD47 pKa = 3.57SLVPDD52 pKa = 5.09DD53 pKa = 5.14SISMVGSVAASTVTTRR69 pKa = 11.84VTRR72 pKa = 11.84GHH74 pKa = 6.76AKK76 pKa = 10.36GSAHH80 pKa = 6.08TVTTPAEE87 pKa = 3.82WHH89 pKa = 6.09ALTRR93 pKa = 11.84EE94 pKa = 4.28DD95 pKa = 6.27KK96 pKa = 10.91LATWLAVCPNEE107 pKa = 3.95TAEE110 pKa = 4.19VLRR113 pKa = 11.84ADD115 pKa = 4.56RR116 pKa = 11.84RR117 pKa = 11.84RR118 pKa = 11.84KK119 pKa = 8.27GLKK122 pKa = 9.88VVVEE126 pKa = 4.22RR127 pKa = 11.84LDD129 pKa = 3.11RR130 pKa = 11.84WFEE133 pKa = 4.01RR134 pKa = 11.84GGAGYY139 pKa = 8.13MATHH143 pKa = 7.42KK144 pKa = 10.86AA145 pKa = 3.4

Molecular weight: 15.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4JU77|R4JU77_9VIRU Uncharacterized protein OS=Cryphonectria parasitica bipartite mycovirus 1 OX=1329781 PE=4 SV=1
MM1 pKa = 7.93ADD3 pKa = 3.48TTPSFDD9 pKa = 4.32DD10 pKa = 4.37LFAGGQQQVDD20 pKa = 4.61FSAPEE25 pKa = 3.85GGIAAVLPVPDD36 pKa = 4.27EE37 pKa = 4.17TDD39 pKa = 3.23NLQTTARR46 pKa = 11.84KK47 pKa = 8.67LAALAAIVEE56 pKa = 4.47RR57 pKa = 11.84GKK59 pKa = 10.83SLAVHH64 pKa = 6.32TGYY67 pKa = 11.15AGAAHH72 pKa = 7.23ATRR75 pKa = 11.84DD76 pKa = 3.85PVDD79 pKa = 3.49LAKK82 pKa = 10.33TSMTPAEE89 pKa = 4.31VKK91 pKa = 10.46QYY93 pKa = 7.03EE94 pKa = 4.11TWARR98 pKa = 11.84GIRR101 pKa = 11.84MPEE104 pKa = 3.74FNFVTNRR111 pKa = 11.84EE112 pKa = 4.07PLRR115 pKa = 11.84GQEE118 pKa = 4.39GARR121 pKa = 11.84KK122 pKa = 9.23RR123 pKa = 11.84YY124 pKa = 7.57EE125 pKa = 3.7RR126 pKa = 11.84RR127 pKa = 11.84ARR129 pKa = 11.84AMNKK133 pKa = 8.01IWKK136 pKa = 8.09TNVAVAEE143 pKa = 4.0NAMFVSIHH151 pKa = 5.32LTYY154 pKa = 9.95MVPLVAAVSKK164 pKa = 10.94IEE166 pKa = 3.91AAEE169 pKa = 4.04MEE171 pKa = 4.58LVGGSNDD178 pKa = 3.4LTEE181 pKa = 4.29MEE183 pKa = 4.24LAEE186 pKa = 4.35VQTAHH191 pKa = 7.57KK192 pKa = 8.26ITSIASANMNSMMEE206 pKa = 4.0QIRR209 pKa = 11.84RR210 pKa = 11.84LTSSINSSKK219 pKa = 10.69EE220 pKa = 3.37IIQARR225 pKa = 11.84EE226 pKa = 3.4HH227 pKa = 6.12AIKK230 pKa = 10.52RR231 pKa = 11.84KK232 pKa = 8.19IQGYY236 pKa = 8.92KK237 pKa = 9.36PKK239 pKa = 10.09KK240 pKa = 10.36DD241 pKa = 4.3PINDD245 pKa = 2.83ARR247 pKa = 11.84QYY249 pKa = 10.93LGEE252 pKa = 4.35APIGDD257 pKa = 3.66EE258 pKa = 3.56VDD260 pKa = 3.24YY261 pKa = 11.44RR262 pKa = 11.84GATRR266 pKa = 11.84GARR269 pKa = 11.84EE270 pKa = 4.69DD271 pKa = 3.61RR272 pKa = 11.84TDD274 pKa = 3.37MLGGILGGTNQGALGGGPSNPQIQRR299 pKa = 11.84QARR302 pKa = 11.84ATGARR307 pKa = 11.84PTAPPVEE314 pKa = 4.06KK315 pKa = 10.01RR316 pKa = 11.84ARR318 pKa = 11.84RR319 pKa = 11.84TGPTRR324 pKa = 11.84EE325 pKa = 4.41DD326 pKa = 3.04IEE328 pKa = 4.2MEE330 pKa = 4.29YY331 pKa = 11.33

Molecular weight: 36.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1068

145

592

356.0

39.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.581 ± 1.524

0.655 ± 0.302

5.993 ± 0.526

5.993 ± 0.88

3.09 ± 0.794

6.648 ± 0.697

2.06 ± 0.256

4.494 ± 0.555

4.401 ± 0.245

7.959 ± 1.339

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.09 ± 0.42

3.09 ± 0.456

5.993 ± 0.416

4.775 ± 0.346

8.24 ± 0.215

5.618 ± 0.482

6.273 ± 1.416

5.993 ± 0.497

1.779 ± 0.524

3.277 ± 0.793
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski