Streptococcus phage CHPC640

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Brussowvirus; unclassified Brussowvirus

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8F962|A0A3G8F962_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC640 OX=2365037 GN=CHPC640_0039 PE=4 SV=1
MM1 pKa = 7.11VNWVDD6 pKa = 4.52ADD8 pKa = 4.28GNDD11 pKa = 4.32LPDD14 pKa = 3.91GTDD17 pKa = 3.28QEE19 pKa = 5.22FKK21 pKa = 11.07AGMYY25 pKa = 10.2FSFAGDD31 pKa = 3.52EE32 pKa = 4.3VNITDD37 pKa = 3.61TGYY40 pKa = 9.31GGYY43 pKa = 10.32YY44 pKa = 9.25GGYY47 pKa = 8.85YY48 pKa = 9.03YY49 pKa = 10.88RR50 pKa = 11.84KK51 pKa = 9.85FEE53 pKa = 4.13FGQFGTVWLSCWNKK67 pKa = 10.66DD68 pKa = 3.89DD69 pKa = 5.14LVNYY73 pKa = 7.38YY74 pKa = 8.26QQ75 pKa = 4.22

Molecular weight: 8.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8F6I5|A0A3G8F6I5_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC640 OX=2365037 GN=CHPC640_0024 PE=4 SV=1
MM1 pKa = 6.87MAVAGFTQFNAQVMRR16 pKa = 11.84SASGMSALSAAISTMPSSLASVASAANIATSSIMRR51 pKa = 11.84IATSAPRR58 pKa = 11.84IASAMSSAAGQVQSAMQRR76 pKa = 11.84MAQSVQSGGQRR87 pKa = 11.84MIQMGRR93 pKa = 11.84QAGTHH98 pKa = 5.65TGQNIASGIQSSVGAVSSAVGALVDD123 pKa = 3.85AARR126 pKa = 11.84SRR128 pKa = 11.84AMGGVGAMQIVGAMIGKK145 pKa = 9.44GLANGMIASLGAVTAAANALVAQAEE170 pKa = 4.19RR171 pKa = 11.84AAQAKK176 pKa = 9.85ARR178 pKa = 11.84IHH180 pKa = 5.7SPSRR184 pKa = 11.84LFRR187 pKa = 11.84DD188 pKa = 3.1KK189 pKa = 11.32VGIYY193 pKa = 9.76LGQGMAVGIDD203 pKa = 3.14RR204 pKa = 11.84SVKK207 pKa = 8.86YY208 pKa = 10.48VKK210 pKa = 10.54SSIEE214 pKa = 3.89NMVDD218 pKa = 2.85TASRR222 pKa = 11.84YY223 pKa = 9.71AISSRR228 pKa = 11.84EE229 pKa = 3.87LFEE232 pKa = 5.42DD233 pKa = 3.47NNVFDD238 pKa = 3.96SFGWGNIRR246 pKa = 11.84GSVDD250 pKa = 3.0LALRR254 pKa = 11.84DD255 pKa = 4.04DD256 pKa = 4.24DD257 pKa = 5.67RR258 pKa = 11.84MDD260 pKa = 3.98RR261 pKa = 11.84LEE263 pKa = 3.95QALDD267 pKa = 4.57LITEE271 pKa = 4.53LVGRR275 pKa = 11.84PISLNINGRR284 pKa = 11.84EE285 pKa = 3.92FAYY288 pKa = 9.28ATADD292 pKa = 5.01DD293 pKa = 3.42ISGYY297 pKa = 9.67QKK299 pKa = 10.56SQEE302 pKa = 3.96FTYY305 pKa = 11.09KK306 pKa = 10.37RR307 pKa = 11.84MRR309 pKa = 11.84GLNN312 pKa = 3.43

Molecular weight: 32.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

12041

47

1779

218.9

24.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.516 ± 0.825

0.556 ± 0.123

6.22 ± 0.426

6.594 ± 0.595

3.895 ± 0.17

7.117 ± 0.579

1.287 ± 0.161

6.852 ± 0.418

8.205 ± 0.507

7.773 ± 0.342

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.491 ± 0.226

5.606 ± 0.32

2.832 ± 0.245

3.646 ± 0.199

4.244 ± 0.433

6.843 ± 0.532

6.378 ± 0.403

6.478 ± 0.238

1.337 ± 0.183

4.128 ± 0.406
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski