Streptococcus phage Javan575

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 42 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AJC4|A0A4D6AJC4_9CAUD Uncharacterized protein OS=Streptococcus phage Javan575 OX=2548267 GN=Javan575_0040 PE=4 SV=1
MM1 pKa = 7.63PVGLIPKK8 pKa = 8.64EE9 pKa = 3.94FLVTSVGCDD18 pKa = 3.29MVNHH22 pKa = 6.01YY23 pKa = 10.68CLGQSTFDD31 pKa = 3.38FTLNTEE37 pKa = 3.95WMNFF41 pKa = 3.37

Molecular weight: 4.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6ALC5|A0A4D6ALC5_9CAUD Site-specific recombinase OS=Streptococcus phage Javan575 OX=2548267 GN=Javan575_0003 PE=4 SV=1
MM1 pKa = 7.72MYY3 pKa = 10.16TILTCTIMGLWVLIGLYY20 pKa = 9.77FGYY23 pKa = 8.1MTIRR27 pKa = 11.84DD28 pKa = 4.32DD29 pKa = 3.97IRR31 pKa = 11.84NEE33 pKa = 3.84MEE35 pKa = 4.97RR36 pKa = 11.84KK37 pKa = 9.79AKK39 pKa = 10.19LNKK42 pKa = 9.61EE43 pKa = 4.24KK44 pKa = 10.81LSQTPLSRR52 pKa = 11.84KK53 pKa = 9.46NKK55 pKa = 9.68

Molecular weight: 6.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

42

0

42

12755

41

1515

303.7

34.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.562 ± 0.566

0.886 ± 0.171

5.958 ± 0.325

7.37 ± 0.43

3.74 ± 0.239

6.57 ± 0.344

1.882 ± 0.144

6.531 ± 0.294

7.15 ± 0.317

9.102 ± 0.311

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.281 ± 0.205

4.869 ± 0.223

2.987 ± 0.212

4.453 ± 0.257

4.712 ± 0.25

6.813 ± 0.399

6.641 ± 0.591

6.327 ± 0.241

1.521 ± 0.089

3.646 ± 0.361
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski