Lacinutrix sp. CAU 1491
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2986 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4U0EVL1|A0A4U0EVL1_9FLAO TetR/AcrR family transcriptional regulator OS=Lacinutrix sp. CAU 1491 OX=2565367 GN=E5167_08800 PE=4 SV=1
MM1 pKa = 7.65 KK2 pKa = 9.11 KK3 pKa = 6.63 TTYY6 pKa = 10.84 SLYY9 pKa = 10.37 HH10 pKa = 5.94 WMCILLLLVSGSLYY24 pKa = 10.08 SQTVIDD30 pKa = 4.74 SEE32 pKa = 4.55 DD33 pKa = 3.85 FEE35 pKa = 5.19 SGWGIWNDD43 pKa = 3.45 GGSDD47 pKa = 3.64 CFLNTGSILSGANSVNLQDD66 pKa = 5.43 NNNQDD71 pKa = 2.79 SSMYY75 pKa = 10.58 TNDD78 pKa = 3.31 IDD80 pKa = 3.9 LTTFGSVTIVFDD92 pKa = 4.12 FRR94 pKa = 11.84 TSGFNNGHH102 pKa = 6.72 DD103 pKa = 3.8 FFIEE107 pKa = 4.44 FSNNGGASWNATPITTYY124 pKa = 10.68 VRR126 pKa = 11.84 GSNFNNDD133 pKa = 2.78 TTYY136 pKa = 10.5 TGEE139 pKa = 4.15 TATITAGGTYY149 pKa = 9.72 TFTANSRR156 pKa = 11.84 FRR158 pKa = 11.84 FRR160 pKa = 11.84 ADD162 pKa = 3.0 ASQSSDD168 pKa = 3.57 DD169 pKa = 4.54 LYY171 pKa = 10.86 IDD173 pKa = 3.56 NVVMTGYY180 pKa = 10.06 PKK182 pKa = 10.25 TPEE185 pKa = 3.69 INITGLGNTINDD197 pKa = 3.67 GDD199 pKa = 4.19 TTPSVTDD206 pKa = 3.38 DD207 pKa = 3.51 TDD209 pKa = 3.72 FGTVNVAGGTNPNTFTIQNTGTAALSLTGASPYY242 pKa = 9.37 VTISGTNAADD252 pKa = 3.86 FAVTTAPANTIASSGSTTFVITFDD276 pKa = 3.76 PSAAGIRR283 pKa = 11.84 TATVSIANDD292 pKa = 3.55 DD293 pKa = 4.29 SNEE296 pKa = 3.75 NPYY299 pKa = 11.21 NFDD302 pKa = 3.29 IEE304 pKa = 4.72 GNGYY308 pKa = 10.18 VPVPEE313 pKa = 4.99 INVQGNSVDD322 pKa = 4.56 IVDD325 pKa = 4.64 GDD327 pKa = 3.92 NTPSLTDD334 pKa = 3.16 ATDD337 pKa = 3.32 YY338 pKa = 9.61 GTVNVEE344 pKa = 4.18 SLVSRR349 pKa = 11.84 TFTIQNTGTADD360 pKa = 3.56 LTISSITLSNTTDD373 pKa = 3.71 FSIIGTPYY381 pKa = 9.81 ASPVASSGSTTFTVQFNSLTIGTKK405 pKa = 6.92 TTTVTINNNDD415 pKa = 3.64 SDD417 pKa = 3.83 EE418 pKa = 4.25 STYY421 pKa = 11.03 QFDD424 pKa = 3.4 IEE426 pKa = 5.3 ARR428 pKa = 11.84 AEE430 pKa = 4.09 QNFFDD435 pKa = 4.39 SDD437 pKa = 3.39 GDD439 pKa = 4.18 GIYY442 pKa = 10.96 DD443 pKa = 5.19 NIDD446 pKa = 3.01 IDD448 pKa = 4.97 DD449 pKa = 5.02 DD450 pKa = 4.29 NDD452 pKa = 4.8 GILDD456 pKa = 3.84 SEE458 pKa = 4.85 EE459 pKa = 4.09 EE460 pKa = 4.15 LACQVSSTSTVMNYY474 pKa = 10.27 KK475 pKa = 10.13 FLNEE479 pKa = 4.12 TFGEE483 pKa = 4.68 GTNRR487 pKa = 11.84 TTINTTYY494 pKa = 11.03 DD495 pKa = 2.87 ATTTYY500 pKa = 10.35 CYY502 pKa = 10.71 EE503 pKa = 5.29 DD504 pKa = 3.81 GTASCPSLGGIDD516 pKa = 4.94 LNDD519 pKa = 3.47 GEE521 pKa = 4.57 YY522 pKa = 10.82 AVYY525 pKa = 8.74 YY526 pKa = 9.0 TAGTNDD532 pKa = 3.46 GTNNTPNGEE541 pKa = 4.08 VASWVDD547 pKa = 3.39 GLWYY551 pKa = 9.57 TGEE554 pKa = 4.21 DD555 pKa = 3.46 HH556 pKa = 7.58 TSGDD560 pKa = 3.78 TNGRR564 pKa = 11.84 MAMFNASYY572 pKa = 11.07 DD573 pKa = 3.3 PGTFYY578 pKa = 10.62 TATITGALPNVPVTYY593 pKa = 10.62 SFWVLNLDD601 pKa = 3.46 ISTANNINTRR611 pKa = 11.84 IRR613 pKa = 11.84 PNILVEE619 pKa = 4.16 FRR621 pKa = 11.84 DD622 pKa = 3.4 AGGNVLQSITTGDD635 pKa = 3.56 IPPSIEE641 pKa = 3.93 GDD643 pKa = 3.63 PANSWHH649 pKa = 6.46 QFTADD654 pKa = 3.09 LTLNVSEE661 pKa = 4.97 FYY663 pKa = 11.17 VYY665 pKa = 10.18 FINNNPGGLGNDD677 pKa = 3.81 LAIDD681 pKa = 5.14 DD682 pKa = 4.33 IVISQTLCDD691 pKa = 3.97 TDD693 pKa = 3.4 NDD695 pKa = 4.78 GIADD699 pKa = 3.99 VFDD702 pKa = 5.4 LDD704 pKa = 4.48 SDD706 pKa = 3.9 NDD708 pKa = 4.68 GIPDD712 pKa = 3.57 VVEE715 pKa = 4.74 AGLGDD720 pKa = 4.12 LSEE723 pKa = 4.62 GTATLTGIASWVDD736 pKa = 3.19 ANGNGMHH743 pKa = 7.34 DD744 pKa = 3.71 LAEE747 pKa = 4.71 GNSVLDD753 pKa = 3.58 TDD755 pKa = 4.52 GDD757 pKa = 4.29 GVPNYY762 pKa = 10.71 LDD764 pKa = 4.89 LDD766 pKa = 4.08 SDD768 pKa = 3.73 NDD770 pKa = 4.28 TIFDD774 pKa = 3.71 VDD776 pKa = 3.38 EE777 pKa = 4.83 SGAGNTADD785 pKa = 4.17 PSYY788 pKa = 11.4 QNGDD792 pKa = 2.87 GDD794 pKa = 3.75 ITGDD798 pKa = 3.82 GVGDD802 pKa = 4.29 GPDD805 pKa = 3.11 TDD807 pKa = 5.56 AIRR810 pKa = 11.84 EE811 pKa = 3.98 TDD813 pKa = 3.19 IDD815 pKa = 3.92 SDD817 pKa = 3.88 GTSEE821 pKa = 4.23 YY822 pKa = 9.81 FTDD825 pKa = 6.12 GILDD829 pKa = 3.35 IYY831 pKa = 10.96 DD832 pKa = 3.85 YY833 pKa = 11.72 YY834 pKa = 11.75 NGGTFGTAYY843 pKa = 10.48 GNSNQGLGNTYY854 pKa = 10.54 YY855 pKa = 11.31 VLDD858 pKa = 4.47 SDD860 pKa = 4.72 SDD862 pKa = 4.35 GIPDD866 pKa = 3.7 YY867 pKa = 11.04 MDD869 pKa = 3.17 ITSDD873 pKa = 3.25 GVTFDD878 pKa = 4.11 ISHH881 pKa = 6.79 TLYY884 pKa = 11.37 ANLDD888 pKa = 3.77 ANNDD892 pKa = 3.56 GVIDD896 pKa = 3.99 DD897 pKa = 4.71 TNDD900 pKa = 3.36 SEE902 pKa = 5.42 GDD904 pKa = 3.87 GILDD908 pKa = 5.35 LFDD911 pKa = 4.13 TDD913 pKa = 4.42 DD914 pKa = 3.99 AAFGSPRR921 pKa = 11.84 DD922 pKa = 3.77 LDD924 pKa = 4.11 RR925 pKa = 11.84 KK926 pKa = 9.12 LHH928 pKa = 6.43 LYY930 pKa = 10.35 FDD932 pKa = 3.92 GRR934 pKa = 11.84 NDD936 pKa = 3.92 YY937 pKa = 11.02 IEE939 pKa = 4.68 DD940 pKa = 3.61 ASVIGTWSEE949 pKa = 3.72 ATQMGWIKK957 pKa = 10.21 IDD959 pKa = 3.38 PTITGGSKK967 pKa = 9.68 TIMGQDD973 pKa = 2.96 NFYY976 pKa = 9.53 ITLNADD982 pKa = 3.91 LSISATANGTTITNGGALATDD1003 pKa = 3.81 RR1004 pKa = 11.84 WVHH1007 pKa = 6.55 IGATYY1012 pKa = 11.2 SNIDD1016 pKa = 4.13 NIFKK1020 pKa = 10.78 LYY1022 pKa = 10.48 INGLEE1027 pKa = 4.19 VSSSAISGSLPSDD1040 pKa = 3.29 TSSYY1044 pKa = 10.46 TLGRR1048 pKa = 11.84 NPDD1051 pKa = 3.33 TDD1053 pKa = 2.84 SGYY1056 pKa = 10.72 FMGYY1060 pKa = 8.68 IDD1062 pKa = 3.85 EE1063 pKa = 5.28 AKK1065 pKa = 10.81 VFDD1068 pKa = 4.17 KK1069 pKa = 11.22 ALTADD1074 pKa = 4.37 EE1075 pKa = 4.3 YY1076 pKa = 11.32 QKK1078 pKa = 11.19 NVYY1081 pKa = 9.26 QEE1083 pKa = 4.68 IEE1085 pKa = 4.44 DD1086 pKa = 3.72 NTLVRR1091 pKa = 11.84 GAIIPKK1097 pKa = 10.31 NITTLSWSNVQRR1109 pKa = 11.84 YY1110 pKa = 7.47 FRR1112 pKa = 11.84 FDD1114 pKa = 3.18 VYY1116 pKa = 11.2 KK1117 pKa = 10.96 DD1118 pKa = 4.66 DD1119 pKa = 3.93 ITDD1122 pKa = 4.09 DD1123 pKa = 3.98 LTTGTIDD1130 pKa = 3.05 TGTGAKK1136 pKa = 8.98 MYY1138 pKa = 9.81 NFKK1141 pKa = 10.75 IIDD1144 pKa = 3.85 YY1145 pKa = 10.22 QSAPLPFVTQQSGSLPTAVDD1165 pKa = 3.23 IASDD1169 pKa = 4.03 GVNGADD1175 pKa = 4.43 AITYY1179 pKa = 7.95 DD1180 pKa = 3.25 WSIVRR1185 pKa = 11.84 VEE1187 pKa = 4.82 HH1188 pKa = 7.94 DD1189 pKa = 4.66 DD1190 pKa = 2.88 ITYY1193 pKa = 10.77 NDD1195 pKa = 3.92 RR1196 pKa = 11.84 QKK1198 pKa = 11.29 HH1199 pKa = 5.45 LGLFVNEE1206 pKa = 4.69 LDD1208 pKa = 4.37 AGLNPIEE1215 pKa = 5.24 FSVQNDD1221 pKa = 3.58 SEE1223 pKa = 5.83 LNVSWYY1229 pKa = 10.86 LKK1231 pKa = 10.71 LDD1233 pKa = 3.44 GLIDD1237 pKa = 4.62 LEE1239 pKa = 4.89 GEE1241 pKa = 4.26 SQLVQGSDD1249 pKa = 3.08 STLDD1253 pKa = 3.37 INSKK1257 pKa = 8.7 GTIEE1261 pKa = 4.9 RR1262 pKa = 11.84 DD1263 pKa = 3.49 QQGTADD1269 pKa = 4.48 LYY1271 pKa = 10.78 TYY1273 pKa = 9.98 NYY1275 pKa = 7.23 WAAPVGVTSTISNNNNYY1292 pKa = 7.81 TLPDD1296 pKa = 3.65 VLNDD1300 pKa = 3.52 GTTPAIPSSINWLTSGYY1317 pKa = 10.23 DD1318 pKa = 3.55 GTSGSPVGIADD1329 pKa = 3.37 YY1330 pKa = 9.81 WIWKK1334 pKa = 7.23 YY1335 pKa = 11.44 ANRR1338 pKa = 11.84 VSDD1341 pKa = 5.27 NYY1343 pKa = 10.66 PSWQHH1348 pKa = 4.87 VRR1350 pKa = 11.84 STGSLQPGEE1359 pKa = 4.69 GYY1361 pKa = 8.82 TMKK1364 pKa = 10.21 GTTNTSGAIFTEE1376 pKa = 3.92 QNYY1379 pKa = 8.94 VFNGKK1384 pKa = 7.73 PHH1386 pKa = 6.59 NGDD1389 pKa = 2.72 ITLTLSAGNDD1399 pKa = 3.55 YY1400 pKa = 11.19 LIGNPYY1406 pKa = 10.72 ASAIDD1411 pKa = 3.71 ANEE1414 pKa = 4.58 FILDD1418 pKa = 4.36 NISDD1422 pKa = 4.01 GAGRR1426 pKa = 11.84 AGSNIINGTLYY1437 pKa = 10.28 FWEE1440 pKa = 4.83 HH1441 pKa = 6.05 FASSTHH1447 pKa = 6.26 ILSEE1451 pKa = 4.08 YY1452 pKa = 8.82 QGGYY1456 pKa = 8.85 GAYY1459 pKa = 9.19 TLITGTAAINNDD1471 pKa = 2.61 VRR1473 pKa = 11.84 INHH1476 pKa = 6.37 TGGLTSSKK1484 pKa = 10.2 GAPEE1488 pKa = 4.02 RR1489 pKa = 11.84 YY1490 pKa = 9.41 IPVAQGFFVTADD1502 pKa = 3.18 TGGSITFKK1510 pKa = 10.55 NSQRR1514 pKa = 11.84 TFKK1517 pKa = 10.67 TEE1519 pKa = 3.56 AADD1522 pKa = 3.42 PSVFMRR1528 pKa = 11.84 VNDD1531 pKa = 4.21 GKK1533 pKa = 11.19 SKK1535 pKa = 9.96 QKK1537 pKa = 10.3 KK1538 pKa = 9.73 SSVTTSKK1545 pKa = 7.72 TTNDD1549 pKa = 2.84 TRR1551 pKa = 11.84 EE1552 pKa = 4.07 VIKK1555 pKa = 10.78 LYY1557 pKa = 10.53 FDD1559 pKa = 3.63 SPKK1562 pKa = 10.54 GYY1564 pKa = 9.74 HH1565 pKa = 5.53 RR1566 pKa = 11.84 QIAVGIDD1573 pKa = 3.17 SNTTDD1578 pKa = 3.4 GHH1580 pKa = 6.88 DD1581 pKa = 3.27 IGYY1584 pKa = 7.99 DD1585 pKa = 3.21 APLIEE1590 pKa = 5.95 DD1591 pKa = 3.63 NVEE1594 pKa = 3.9 DD1595 pKa = 4.48 MFWTFDD1601 pKa = 3.36 NNKK1604 pKa = 9.42 FVIQGVSDD1612 pKa = 3.71 FNINRR1617 pKa = 11.84 TLPLGVKK1624 pKa = 9.32 IDD1626 pKa = 3.81 QEE1628 pKa = 4.39 GTATIRR1634 pKa = 11.84 VDD1636 pKa = 3.52 YY1637 pKa = 10.8 LEE1639 pKa = 5.54 NIPIDD1644 pKa = 3.75 TNIYY1648 pKa = 10.54 LLDD1651 pKa = 4.35 KK1652 pKa = 10.47 EE1653 pKa = 4.5 LEE1655 pKa = 4.17 IYY1657 pKa = 10.53 HH1658 pKa = 6.96 DD1659 pKa = 3.99 LRR1661 pKa = 11.84 EE1662 pKa = 4.29 SNYY1665 pKa = 10.41 EE1666 pKa = 3.78 VFLTIGEE1673 pKa = 4.27 HH1674 pKa = 5.44 YY1675 pKa = 10.79 DD1676 pKa = 3.14 RR1677 pKa = 11.84 FEE1679 pKa = 3.87 ITFSNGEE1686 pKa = 4.14 EE1687 pKa = 3.93 EE1688 pKa = 4.11 VDD1690 pKa = 4.39 PIEE1693 pKa = 5.42 GLTKK1697 pKa = 10.54 TNLHH1701 pKa = 6.57 FSNDD1705 pKa = 3.35 KK1706 pKa = 10.77 EE1707 pKa = 4.56 SIIVINPDD1715 pKa = 2.98 NLIINSVKK1723 pKa = 9.59 MVNILGQSVFKK1734 pKa = 10.35 MDD1736 pKa = 3.2 TAFEE1740 pKa = 4.12 DD1741 pKa = 4.21 RR1742 pKa = 11.84 YY1743 pKa = 11.16 KK1744 pKa = 10.88 EE1745 pKa = 3.76 ITVKK1749 pKa = 10.55 HH1750 pKa = 6.4 LNTGAYY1756 pKa = 8.64 IINMITNKK1764 pKa = 10.44 GLITKK1769 pKa = 9.9 KK1770 pKa = 10.7 VVVEE1774 pKa = 4.07
Molecular weight: 192.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.677
IPC2_protein 3.77
IPC_protein 3.834
Toseland 3.592
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.783
Rodwell 3.643
Grimsley 3.49
Solomon 3.834
Lehninger 3.783
Nozaki 3.935
DTASelect 4.228
Thurlkill 3.643
EMBOSS 3.783
Sillero 3.948
Patrickios 1.227
IPC_peptide 3.821
IPC2_peptide 3.923
IPC2.peptide.svr19 3.848
Protein with the highest isoelectric point:
>tr|A0A4V5LQD5|A0A4V5LQD5_9FLAO DUF2807 domain-containing protein OS=Lacinutrix sp. CAU 1491 OX=2565367 GN=E5167_10865 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 9.13 RR10 pKa = 11.84 KK11 pKa = 9.48 RR12 pKa = 11.84 RR13 pKa = 11.84 NKK15 pKa = 9.49 HH16 pKa = 3.94 GFRR19 pKa = 11.84 EE20 pKa = 4.27 RR21 pKa = 11.84 MASANGRR28 pKa = 11.84 KK29 pKa = 9.04 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.09 GRR39 pKa = 11.84 KK40 pKa = 7.97 KK41 pKa = 10.66 LSVSSEE47 pKa = 3.9 LRR49 pKa = 11.84 HH50 pKa = 6.16 KK51 pKa = 10.53 KK52 pKa = 10.04
Molecular weight: 6.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 10.76
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.296
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.018
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.066
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2986
0
2986
998528
28
2381
334.4
37.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.024 ± 0.048
0.719 ± 0.013
5.624 ± 0.035
6.421 ± 0.044
5.207 ± 0.035
6.132 ± 0.046
1.817 ± 0.018
8.214 ± 0.045
8.226 ± 0.053
9.215 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.19 ± 0.023
6.684 ± 0.049
3.261 ± 0.022
3.255 ± 0.023
3.32 ± 0.029
6.555 ± 0.037
5.799 ± 0.042
6.145 ± 0.031
1.049 ± 0.016
4.14 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here