Amino acid dipepetide frequency for Pseudomonas phage Ps59

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.504AlaAla: 17.504 ± 3.615
1.109AlaCys: 1.109 ± 0.344
6.811AlaAsp: 6.811 ± 0.648
9.187AlaGlu: 9.187 ± 0.875
3.168AlaPhe: 3.168 ± 0.57
10.771AlaGly: 10.771 ± 1.212
2.138AlaHis: 2.138 ± 0.466
4.673AlaIle: 4.673 ± 0.404
4.99AlaLys: 4.99 ± 0.821
11.88AlaLeu: 11.88 ± 0.972
3.406AlaMet: 3.406 ± 0.432
3.089AlaAsn: 3.089 ± 0.499
4.99AlaPro: 4.99 ± 0.623
5.623AlaGln: 5.623 ± 0.818
7.128AlaArg: 7.128 ± 0.745
6.415AlaSer: 6.415 ± 0.693
6.019AlaThr: 6.019 ± 0.779
6.574AlaVal: 6.574 ± 0.759
2.772AlaTrp: 2.772 ± 0.428
2.059AlaTyr: 2.059 ± 0.349
0.0AlaXaa: 0.0 ± 0.0
Cys
0.554CysAla: 0.554 ± 0.22
0.317CysCys: 0.317 ± 0.156
0.475CysAsp: 0.475 ± 0.193
0.475CysGlu: 0.475 ± 0.183
0.079CysPhe: 0.079 ± 0.082
0.634CysGly: 0.634 ± 0.197
0.079CysHis: 0.079 ± 0.087
0.158CysIle: 0.158 ± 0.127
0.317CysLys: 0.317 ± 0.161
0.713CysLeu: 0.713 ± 0.223
0.158CysMet: 0.158 ± 0.126
0.396CysAsn: 0.396 ± 0.2
0.634CysPro: 0.634 ± 0.242
0.475CysGln: 0.475 ± 0.215
0.634CysArg: 0.634 ± 0.217
0.871CysSer: 0.871 ± 0.282
0.475CysThr: 0.475 ± 0.211
0.554CysVal: 0.554 ± 0.218
0.079CysTrp: 0.079 ± 0.078
0.317CysTyr: 0.317 ± 0.153
0.0CysXaa: 0.0 ± 0.0
Asp
6.257AspAla: 6.257 ± 0.711
0.158AspCys: 0.158 ± 0.113
3.326AspAsp: 3.326 ± 0.472
4.277AspGlu: 4.277 ± 0.567
1.98AspPhe: 1.98 ± 0.413
5.307AspGly: 5.307 ± 0.639
1.346AspHis: 1.346 ± 0.301
2.693AspIle: 2.693 ± 0.502
1.346AspLys: 1.346 ± 0.315
5.782AspLeu: 5.782 ± 0.551
1.188AspMet: 1.188 ± 0.271
1.346AspAsn: 1.346 ± 0.307
2.534AspPro: 2.534 ± 0.48
3.881AspGln: 3.881 ± 0.643
4.356AspArg: 4.356 ± 0.604
2.772AspSer: 2.772 ± 0.426
2.455AspThr: 2.455 ± 0.378
4.514AspVal: 4.514 ± 0.537
0.871AspTrp: 0.871 ± 0.234
1.584AspTyr: 1.584 ± 0.329
0.0AspXaa: 0.0 ± 0.0
Glu
6.099GluAla: 6.099 ± 0.939
0.95GluCys: 0.95 ± 0.295
3.168GluAsp: 3.168 ± 0.556
3.722GluGlu: 3.722 ± 0.64
1.98GluPhe: 1.98 ± 0.357
2.93GluGly: 2.93 ± 0.422
1.742GluHis: 1.742 ± 0.402
2.93GluIle: 2.93 ± 0.491
3.01GluLys: 3.01 ± 0.55
7.92GluLeu: 7.92 ± 0.742
1.346GluMet: 1.346 ± 0.277
2.297GluAsn: 2.297 ± 0.405
2.297GluPro: 2.297 ± 0.547
4.752GluGln: 4.752 ± 0.726
5.623GluArg: 5.623 ± 0.821
3.802GluSer: 3.802 ± 0.549
2.218GluThr: 2.218 ± 0.346
4.039GluVal: 4.039 ± 0.56
1.426GluTrp: 1.426 ± 0.312
1.584GluTyr: 1.584 ± 0.3
0.0GluXaa: 0.0 ± 0.0
Phe
3.089PheAla: 3.089 ± 0.474
0.317PheCys: 0.317 ± 0.172
2.851PheAsp: 2.851 ± 0.481
1.98PheGlu: 1.98 ± 0.401
0.792PhePhe: 0.792 ± 0.226
2.851PheGly: 2.851 ± 0.474
0.475PheHis: 0.475 ± 0.156
1.109PheIle: 1.109 ± 0.222
0.713PheLys: 0.713 ± 0.257
2.614PheLeu: 2.614 ± 0.423
0.475PheMet: 0.475 ± 0.186
1.426PheAsn: 1.426 ± 0.405
0.95PhePro: 0.95 ± 0.291
1.346PheGln: 1.346 ± 0.333
2.614PheArg: 2.614 ± 0.468
1.109PheSer: 1.109 ± 0.217
1.822PheThr: 1.822 ± 0.374
1.505PheVal: 1.505 ± 0.324
0.634PheTrp: 0.634 ± 0.262
0.792PheTyr: 0.792 ± 0.281
0.0PheXaa: 0.0 ± 0.0
Gly
8.395GlyAla: 8.395 ± 1.538
0.95GlyCys: 0.95 ± 0.303
4.277GlyAsp: 4.277 ± 0.512
3.802GlyGlu: 3.802 ± 0.432
2.693GlyPhe: 2.693 ± 0.503
6.019GlyGly: 6.019 ± 0.626
1.109GlyHis: 1.109 ± 0.357
3.406GlyIle: 3.406 ± 0.438
3.96GlyLys: 3.96 ± 0.551
7.999GlyLeu: 7.999 ± 0.995
1.267GlyMet: 1.267 ± 0.274
3.247GlyAsn: 3.247 ± 0.528
2.059GlyPro: 2.059 ± 0.448
4.435GlyGln: 4.435 ± 0.627
5.703GlyArg: 5.703 ± 0.682
3.643GlySer: 3.643 ± 0.575
4.594GlyThr: 4.594 ± 0.654
4.911GlyVal: 4.911 ± 0.559
2.059GlyTrp: 2.059 ± 0.362
2.297GlyTyr: 2.297 ± 0.51
0.0GlyXaa: 0.0 ± 0.0
His
1.663HisAla: 1.663 ± 0.35
0.079HisCys: 0.079 ± 0.083
1.346HisAsp: 1.346 ± 0.321
1.03HisGlu: 1.03 ± 0.253
0.396HisPhe: 0.396 ± 0.133
1.742HisGly: 1.742 ± 0.466
0.317HisHis: 0.317 ± 0.181
0.871HisIle: 0.871 ± 0.25
0.396HisLys: 0.396 ± 0.153
2.059HisLeu: 2.059 ± 0.549
0.317HisMet: 0.317 ± 0.17
0.713HisAsn: 0.713 ± 0.244
1.188HisPro: 1.188 ± 0.382
0.871HisGln: 0.871 ± 0.286
1.346HisArg: 1.346 ± 0.27
1.426HisSer: 1.426 ± 0.279
0.713HisThr: 0.713 ± 0.221
1.267HisVal: 1.267 ± 0.282
0.158HisTrp: 0.158 ± 0.108
0.317HisTyr: 0.317 ± 0.141
0.0HisXaa: 0.0 ± 0.0
Ile
5.227IleAla: 5.227 ± 0.736
0.238IleCys: 0.238 ± 0.171
3.485IleAsp: 3.485 ± 0.466
3.881IleGlu: 3.881 ± 0.594
0.792IlePhe: 0.792 ± 0.221
2.059IleGly: 2.059 ± 0.343
1.109IleHis: 1.109 ± 0.289
1.267IleIle: 1.267 ± 0.395
1.03IleLys: 1.03 ± 0.219
3.168IleLeu: 3.168 ± 0.518
0.713IleMet: 0.713 ± 0.235
1.742IleAsn: 1.742 ± 0.365
2.059IlePro: 2.059 ± 0.412
1.901IleGln: 1.901 ± 0.404
3.089IleArg: 3.089 ± 0.382
2.614IleSer: 2.614 ± 0.523
3.089IleThr: 3.089 ± 0.563
2.297IleVal: 2.297 ± 0.429
0.792IleTrp: 0.792 ± 0.35
1.03IleTyr: 1.03 ± 0.29
0.0IleXaa: 0.0 ± 0.0
Lys
6.178LysAla: 6.178 ± 0.984
0.158LysCys: 0.158 ± 0.121
2.218LysAsp: 2.218 ± 0.453
2.614LysGlu: 2.614 ± 0.405
0.871LysPhe: 0.871 ± 0.208
2.376LysGly: 2.376 ± 0.606
0.634LysHis: 0.634 ± 0.216
1.188LysIle: 1.188 ± 0.348
1.742LysLys: 1.742 ± 0.336
3.247LysLeu: 3.247 ± 0.599
0.317LysMet: 0.317 ± 0.158
1.188LysAsn: 1.188 ± 0.293
1.98LysPro: 1.98 ± 0.475
1.267LysGln: 1.267 ± 0.435
3.881LysArg: 3.881 ± 0.577
1.98LysSer: 1.98 ± 0.43
2.297LysThr: 2.297 ± 0.474
1.901LysVal: 1.901 ± 0.469
0.396LysTrp: 0.396 ± 0.155
0.634LysTyr: 0.634 ± 0.175
0.0LysXaa: 0.0 ± 0.0
Leu
13.068LeuAla: 13.068 ± 0.964
1.188LeuCys: 1.188 ± 0.379
7.366LeuAsp: 7.366 ± 0.731
5.307LeuGlu: 5.307 ± 0.638
2.455LeuPhe: 2.455 ± 0.526
7.445LeuGly: 7.445 ± 0.743
2.059LeuHis: 2.059 ± 0.416
4.514LeuIle: 4.514 ± 0.674
3.96LeuLys: 3.96 ± 0.733
9.504LeuLeu: 9.504 ± 0.796
1.822LeuMet: 1.822 ± 0.281
3.247LeuAsn: 3.247 ± 0.527
5.148LeuPro: 5.148 ± 0.523
5.227LeuGln: 5.227 ± 0.94
7.841LeuArg: 7.841 ± 0.628
5.782LeuSer: 5.782 ± 0.77
5.94LeuThr: 5.94 ± 0.759
7.841LeuVal: 7.841 ± 0.76
0.95LeuTrp: 0.95 ± 0.265
2.376LeuTyr: 2.376 ± 0.452
0.0LeuXaa: 0.0 ± 0.0
Met
2.93MetAla: 2.93 ± 0.477
0.079MetCys: 0.079 ± 0.08
0.95MetAsp: 0.95 ± 0.289
1.505MetGlu: 1.505 ± 0.342
0.634MetPhe: 0.634 ± 0.174
2.138MetGly: 2.138 ± 0.44
0.079MetHis: 0.079 ± 0.069
0.871MetIle: 0.871 ± 0.234
0.871MetLys: 0.871 ± 0.261
1.267MetLeu: 1.267 ± 0.273
0.317MetMet: 0.317 ± 0.145
0.95MetAsn: 0.95 ± 0.356
1.109MetPro: 1.109 ± 0.276
1.267MetGln: 1.267 ± 0.291
1.267MetArg: 1.267 ± 0.33
1.426MetSer: 1.426 ± 0.276
1.663MetThr: 1.663 ± 0.429
0.871MetVal: 0.871 ± 0.297
0.317MetTrp: 0.317 ± 0.141
0.158MetTyr: 0.158 ± 0.157
0.0MetXaa: 0.0 ± 0.0
Asn
3.485AsnAla: 3.485 ± 0.541
0.238AsnCys: 0.238 ± 0.181
1.346AsnAsp: 1.346 ± 0.303
2.059AsnGlu: 2.059 ± 0.334
0.871AsnPhe: 0.871 ± 0.233
3.247AsnGly: 3.247 ± 0.759
0.634AsnHis: 0.634 ± 0.238
1.109AsnIle: 1.109 ± 0.333
0.713AsnLys: 0.713 ± 0.231
2.93AsnLeu: 2.93 ± 0.589
0.475AsnMet: 0.475 ± 0.166
0.95AsnAsn: 0.95 ± 0.339
1.505AsnPro: 1.505 ± 0.317
1.901AsnGln: 1.901 ± 0.429
2.534AsnArg: 2.534 ± 0.472
1.505AsnSer: 1.505 ± 0.36
2.138AsnThr: 2.138 ± 0.517
2.059AsnVal: 2.059 ± 0.376
0.713AsnTrp: 0.713 ± 0.219
0.713AsnTyr: 0.713 ± 0.203
0.0AsnXaa: 0.0 ± 0.0
Pro
6.019ProAla: 6.019 ± 0.796
0.396ProCys: 0.396 ± 0.187
3.01ProAsp: 3.01 ± 0.574
3.168ProGlu: 3.168 ± 0.492
1.742ProPhe: 1.742 ± 0.352
4.198ProGly: 4.198 ± 0.565
0.634ProHis: 0.634 ± 0.223
1.584ProIle: 1.584 ± 0.292
1.505ProLys: 1.505 ± 0.364
3.96ProLeu: 3.96 ± 0.714
1.267ProMet: 1.267 ± 0.317
0.792ProAsn: 0.792 ± 0.206
2.059ProPro: 2.059 ± 0.534
1.584ProGln: 1.584 ± 0.296
3.406ProArg: 3.406 ± 0.449
3.01ProSer: 3.01 ± 0.53
2.772ProThr: 2.772 ± 0.391
4.039ProVal: 4.039 ± 0.832
0.792ProTrp: 0.792 ± 0.258
1.584ProTyr: 1.584 ± 0.392
0.0ProXaa: 0.0 ± 0.0
Gln
6.891GlnAla: 6.891 ± 1.17
0.317GlnCys: 0.317 ± 0.179
2.218GlnAsp: 2.218 ± 0.434
3.247GlnGlu: 3.247 ± 0.767
1.901GlnPhe: 1.901 ± 0.411
2.297GlnGly: 2.297 ± 0.394
0.713GlnHis: 0.713 ± 0.182
2.218GlnIle: 2.218 ± 0.415
1.584GlnLys: 1.584 ± 0.655
7.366GlnLeu: 7.366 ± 0.824
0.871GlnMet: 0.871 ± 0.261
0.95GlnAsn: 0.95 ± 0.311
3.01GlnPro: 3.01 ± 0.538
2.772GlnGln: 2.772 ± 0.728
3.643GlnArg: 3.643 ± 0.498
2.297GlnSer: 2.297 ± 0.405
2.376GlnThr: 2.376 ± 0.377
3.802GlnVal: 3.802 ± 0.47
0.871GlnTrp: 0.871 ± 0.238
1.188GlnTyr: 1.188 ± 0.329
0.0GlnXaa: 0.0 ± 0.0
Arg
7.287ArgAla: 7.287 ± 0.662
0.396ArgCys: 0.396 ± 0.184
4.831ArgAsp: 4.831 ± 0.484
4.752ArgGlu: 4.752 ± 0.604
2.138ArgPhe: 2.138 ± 0.38
4.752ArgGly: 4.752 ± 0.705
1.663ArgHis: 1.663 ± 0.428
3.326ArgIle: 3.326 ± 0.729
3.485ArgLys: 3.485 ± 0.858
7.999ArgLeu: 7.999 ± 0.71
1.505ArgMet: 1.505 ± 0.342
1.663ArgAsn: 1.663 ± 0.384
3.089ArgPro: 3.089 ± 0.62
3.802ArgGln: 3.802 ± 0.731
5.703ArgArg: 5.703 ± 0.78
4.039ArgSer: 4.039 ± 0.5
2.93ArgThr: 2.93 ± 0.448
4.99ArgVal: 4.99 ± 0.633
1.505ArgTrp: 1.505 ± 0.404
2.376ArgTyr: 2.376 ± 0.444
0.0ArgXaa: 0.0 ± 0.0
Ser
8.158SerAla: 8.158 ± 0.816
0.317SerCys: 0.317 ± 0.155
2.772SerAsp: 2.772 ± 0.442
2.455SerGlu: 2.455 ± 0.447
2.059SerPhe: 2.059 ± 0.375
4.594SerGly: 4.594 ± 0.495
0.554SerHis: 0.554 ± 0.283
2.614SerIle: 2.614 ± 0.429
2.297SerLys: 2.297 ± 0.485
6.732SerLeu: 6.732 ± 0.572
1.267SerMet: 1.267 ± 0.287
1.584SerAsn: 1.584 ± 0.404
2.93SerPro: 2.93 ± 0.6
1.663SerGln: 1.663 ± 0.381
3.643SerArg: 3.643 ± 0.582
3.802SerSer: 3.802 ± 0.729
3.326SerThr: 3.326 ± 0.481
3.881SerVal: 3.881 ± 0.606
1.03SerTrp: 1.03 ± 0.296
1.584SerTyr: 1.584 ± 0.335
0.0SerXaa: 0.0 ± 0.0
Thr
7.128ThrAla: 7.128 ± 0.722
0.475ThrCys: 0.475 ± 0.186
1.822ThrAsp: 1.822 ± 0.422
3.247ThrGlu: 3.247 ± 0.466
1.663ThrPhe: 1.663 ± 0.342
5.861ThrGly: 5.861 ± 1.016
0.95ThrHis: 0.95 ± 0.264
1.742ThrIle: 1.742 ± 0.388
1.901ThrLys: 1.901 ± 0.42
5.861ThrLeu: 5.861 ± 0.672
0.95ThrMet: 0.95 ± 0.218
1.98ThrAsn: 1.98 ± 0.451
3.722ThrPro: 3.722 ± 0.375
2.138ThrGln: 2.138 ± 0.429
2.693ThrArg: 2.693 ± 0.414
3.881ThrSer: 3.881 ± 0.53
3.564ThrThr: 3.564 ± 0.641
3.485ThrVal: 3.485 ± 0.642
0.713ThrTrp: 0.713 ± 0.288
1.584ThrTyr: 1.584 ± 0.338
0.0ThrXaa: 0.0 ± 0.0
Val
6.574ValAla: 6.574 ± 0.859
0.396ValCys: 0.396 ± 0.146
4.039ValAsp: 4.039 ± 0.518
4.99ValGlu: 4.99 ± 0.601
1.98ValPhe: 1.98 ± 0.352
4.277ValGly: 4.277 ± 0.572
1.188ValHis: 1.188 ± 0.34
3.406ValIle: 3.406 ± 0.483
2.059ValLys: 2.059 ± 0.465
7.524ValLeu: 7.524 ± 0.816
1.188ValMet: 1.188 ± 0.287
1.901ValAsn: 1.901 ± 0.384
4.039ValPro: 4.039 ± 0.519
3.564ValGln: 3.564 ± 0.473
3.247ValArg: 3.247 ± 0.492
4.277ValSer: 4.277 ± 0.585
4.356ValThr: 4.356 ± 0.467
5.069ValVal: 5.069 ± 0.794
1.267ValTrp: 1.267 ± 0.37
1.109ValTyr: 1.109 ± 0.268
0.0ValXaa: 0.0 ± 0.0
Trp
1.584TrpAla: 1.584 ± 0.361
0.158TrpCys: 0.158 ± 0.105
0.713TrpAsp: 0.713 ± 0.253
0.238TrpGlu: 0.238 ± 0.139
0.871TrpPhe: 0.871 ± 0.206
0.871TrpGly: 0.871 ± 0.258
0.317TrpHis: 0.317 ± 0.17
1.188TrpIle: 1.188 ± 0.358
0.317TrpLys: 0.317 ± 0.15
2.614TrpLeu: 2.614 ± 0.475
0.792TrpMet: 0.792 ± 0.263
0.634TrpAsn: 0.634 ± 0.207
0.792TrpPro: 0.792 ± 0.321
1.267TrpGln: 1.267 ± 0.265
1.426TrpArg: 1.426 ± 0.276
1.03TrpSer: 1.03 ± 0.334
1.426TrpThr: 1.426 ± 0.365
1.267TrpVal: 1.267 ± 0.287
0.792TrpTrp: 0.792 ± 0.325
0.158TrpTyr: 0.158 ± 0.1
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.218TyrAla: 2.218 ± 0.429
0.158TyrCys: 0.158 ± 0.117
0.95TyrAsp: 0.95 ± 0.238
1.98TyrGlu: 1.98 ± 0.342
0.396TyrPhe: 0.396 ± 0.161
2.455TyrGly: 2.455 ± 0.36
0.475TyrHis: 0.475 ± 0.292
0.713TyrIle: 0.713 ± 0.233
0.871TyrLys: 0.871 ± 0.257
1.901TyrLeu: 1.901 ± 0.42
0.95TyrMet: 0.95 ± 0.27
1.03TyrAsn: 1.03 ± 0.332
1.426TyrPro: 1.426 ± 0.434
0.792TyrGln: 0.792 ± 0.224
2.455TyrArg: 2.455 ± 0.353
1.584TyrSer: 1.584 ± 0.401
1.188TyrThr: 1.188 ± 0.295
1.584TyrVal: 1.584 ± 0.379
0.317TyrTrp: 0.317 ± 0.164
0.238TyrTyr: 0.238 ± 0.11
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (12627 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski