Amino acid dipepetide frequency for Pseudomonas phage DDSR119

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.179AlaAla: 13.179 ± 1.721
1.232AlaCys: 1.232 ± 0.312
5.85AlaAsp: 5.85 ± 0.672
7.76AlaGlu: 7.76 ± 1.014
3.633AlaPhe: 3.633 ± 0.529
7.021AlaGly: 7.021 ± 0.734
1.478AlaHis: 1.478 ± 0.348
6.343AlaIle: 6.343 ± 0.686
6.897AlaLys: 6.897 ± 0.833
8.807AlaLeu: 8.807 ± 0.737
3.018AlaMet: 3.018 ± 0.404
3.941AlaAsn: 3.941 ± 0.592
4.003AlaPro: 4.003 ± 0.597
4.126AlaGln: 4.126 ± 0.504
7.39AlaArg: 7.39 ± 0.705
5.173AlaSer: 5.173 ± 0.623
6.035AlaThr: 6.035 ± 0.881
6.836AlaVal: 6.836 ± 0.788
1.416AlaTrp: 1.416 ± 0.313
3.079AlaTyr: 3.079 ± 0.455
0.0AlaXaa: 0.0 ± 0.0
Cys
1.17CysAla: 1.17 ± 0.271
0.308CysCys: 0.308 ± 0.142
0.739CysAsp: 0.739 ± 0.198
0.616CysGlu: 0.616 ± 0.175
0.431CysPhe: 0.431 ± 0.186
1.54CysGly: 1.54 ± 0.37
0.308CysHis: 0.308 ± 0.132
0.739CysIle: 0.739 ± 0.237
0.985CysLys: 0.985 ± 0.275
0.739CysLeu: 0.739 ± 0.25
0.493CysMet: 0.493 ± 0.173
0.246CysAsn: 0.246 ± 0.116
0.616CysPro: 0.616 ± 0.222
0.431CysGln: 0.431 ± 0.189
0.739CysArg: 0.739 ± 0.23
0.185CysSer: 0.185 ± 0.101
0.493CysThr: 0.493 ± 0.194
0.862CysVal: 0.862 ± 0.22
0.185CysTrp: 0.185 ± 0.126
0.493CysTyr: 0.493 ± 0.199
0.0CysXaa: 0.0 ± 0.0
Asp
5.604AspAla: 5.604 ± 0.605
0.801AspCys: 0.801 ± 0.195
4.372AspAsp: 4.372 ± 0.599
4.249AspGlu: 4.249 ± 0.5
1.848AspPhe: 1.848 ± 0.388
5.296AspGly: 5.296 ± 0.71
1.17AspHis: 1.17 ± 0.259
3.387AspIle: 3.387 ± 0.502
3.449AspLys: 3.449 ± 0.464
4.496AspLeu: 4.496 ± 0.526
2.094AspMet: 2.094 ± 0.432
2.217AspAsn: 2.217 ± 0.385
3.695AspPro: 3.695 ± 0.505
2.402AspGln: 2.402 ± 0.428
3.941AspArg: 3.941 ± 0.385
2.956AspSer: 2.956 ± 0.417
3.141AspThr: 3.141 ± 0.311
4.065AspVal: 4.065 ± 0.431
1.047AspTrp: 1.047 ± 0.257
1.909AspTyr: 1.909 ± 0.346
0.0AspXaa: 0.0 ± 0.0
Glu
7.76GluAla: 7.76 ± 0.751
0.677GluCys: 0.677 ± 0.203
2.279GluAsp: 2.279 ± 0.407
3.633GluGlu: 3.633 ± 0.502
3.88GluPhe: 3.88 ± 0.619
4.311GluGly: 4.311 ± 0.57
1.786GluHis: 1.786 ± 0.346
4.065GluIle: 4.065 ± 0.506
3.326GluLys: 3.326 ± 0.599
5.419GluLeu: 5.419 ± 0.518
1.416GluMet: 1.416 ± 0.242
2.155GluAsn: 2.155 ± 0.435
2.894GluPro: 2.894 ± 0.647
3.387GluGln: 3.387 ± 0.542
3.941GluArg: 3.941 ± 0.535
2.771GluSer: 2.771 ± 0.418
3.141GluThr: 3.141 ± 0.493
4.496GluVal: 4.496 ± 0.527
1.355GluTrp: 1.355 ± 0.331
1.047GluTyr: 1.047 ± 0.318
0.0GluXaa: 0.0 ± 0.0
Phe
3.633PheAla: 3.633 ± 0.424
0.246PheCys: 0.246 ± 0.108
2.155PheAsp: 2.155 ± 0.422
2.094PheGlu: 2.094 ± 0.338
0.862PhePhe: 0.862 ± 0.25
3.141PheGly: 3.141 ± 0.411
0.985PheHis: 0.985 ± 0.237
1.232PheIle: 1.232 ± 0.298
1.971PheLys: 1.971 ± 0.418
1.786PheLeu: 1.786 ± 0.293
0.862PheMet: 0.862 ± 0.254
2.032PheAsn: 2.032 ± 0.332
0.985PhePro: 0.985 ± 0.223
0.924PheGln: 0.924 ± 0.233
1.54PheArg: 1.54 ± 0.325
2.032PheSer: 2.032 ± 0.423
1.909PheThr: 1.909 ± 0.427
2.217PheVal: 2.217 ± 0.311
0.246PheTrp: 0.246 ± 0.145
0.862PheTyr: 0.862 ± 0.19
0.0PheXaa: 0.0 ± 0.0
Gly
7.021GlyAla: 7.021 ± 0.852
0.924GlyCys: 0.924 ± 0.256
4.557GlyAsp: 4.557 ± 0.569
5.111GlyGlu: 5.111 ± 0.628
3.141GlyPhe: 3.141 ± 0.46
6.097GlyGly: 6.097 ± 0.883
1.293GlyHis: 1.293 ± 0.281
2.71GlyIle: 2.71 ± 0.52
4.742GlyLys: 4.742 ± 0.537
5.912GlyLeu: 5.912 ± 0.805
3.079GlyMet: 3.079 ± 0.418
3.141GlyAsn: 3.141 ± 0.511
2.833GlyPro: 2.833 ± 0.357
3.326GlyGln: 3.326 ± 0.397
5.358GlyArg: 5.358 ± 0.608
4.865GlySer: 4.865 ± 0.694
4.804GlyThr: 4.804 ± 0.624
5.666GlyVal: 5.666 ± 0.637
1.109GlyTrp: 1.109 ± 0.295
2.894GlyTyr: 2.894 ± 0.395
0.0GlyXaa: 0.0 ± 0.0
His
1.786HisAla: 1.786 ± 0.417
0.431HisCys: 0.431 ± 0.167
1.047HisAsp: 1.047 ± 0.348
1.293HisGlu: 1.293 ± 0.325
0.493HisPhe: 0.493 ± 0.169
1.416HisGly: 1.416 ± 0.297
0.493HisHis: 0.493 ± 0.205
0.801HisIle: 0.801 ± 0.214
1.663HisLys: 1.663 ± 0.337
1.971HisLeu: 1.971 ± 0.337
0.493HisMet: 0.493 ± 0.198
0.493HisAsn: 0.493 ± 0.176
1.232HisPro: 1.232 ± 0.293
0.801HisGln: 0.801 ± 0.215
1.293HisArg: 1.293 ± 0.28
1.232HisSer: 1.232 ± 0.286
0.924HisThr: 0.924 ± 0.246
1.416HisVal: 1.416 ± 0.319
0.246HisTrp: 0.246 ± 0.138
0.554HisTyr: 0.554 ± 0.151
0.0HisXaa: 0.0 ± 0.0
Ile
4.804IleAla: 4.804 ± 0.614
0.37IleCys: 0.37 ± 0.152
4.742IleAsp: 4.742 ± 0.506
3.695IleGlu: 3.695 ± 0.443
1.601IlePhe: 1.601 ± 0.246
4.557IleGly: 4.557 ± 0.548
0.985IleHis: 0.985 ± 0.241
2.525IleIle: 2.525 ± 0.383
2.833IleLys: 2.833 ± 0.383
3.449IleLeu: 3.449 ± 0.512
0.924IleMet: 0.924 ± 0.279
2.525IleAsn: 2.525 ± 0.372
2.279IlePro: 2.279 ± 0.301
1.909IleGln: 1.909 ± 0.325
3.572IleArg: 3.572 ± 0.542
2.525IleSer: 2.525 ± 0.45
3.572IleThr: 3.572 ± 0.489
3.326IleVal: 3.326 ± 0.507
0.308IleTrp: 0.308 ± 0.137
1.663IleTyr: 1.663 ± 0.371
0.0IleXaa: 0.0 ± 0.0
Lys
7.082LysAla: 7.082 ± 0.994
1.109LysCys: 1.109 ± 0.303
2.894LysAsp: 2.894 ± 0.455
3.141LysGlu: 3.141 ± 0.574
1.17LysPhe: 1.17 ± 0.283
4.188LysGly: 4.188 ± 0.479
1.17LysHis: 1.17 ± 0.291
2.279LysIle: 2.279 ± 0.415
2.463LysLys: 2.463 ± 0.462
5.111LysLeu: 5.111 ± 0.665
1.724LysMet: 1.724 ± 0.412
1.786LysAsn: 1.786 ± 0.321
2.34LysPro: 2.34 ± 0.371
1.601LysGln: 1.601 ± 0.381
4.188LysArg: 4.188 ± 0.504
2.648LysSer: 2.648 ± 0.383
3.572LysThr: 3.572 ± 0.415
3.633LysVal: 3.633 ± 0.475
1.109LysTrp: 1.109 ± 0.276
1.478LysTyr: 1.478 ± 0.331
0.0LysXaa: 0.0 ± 0.0
Leu
8.807LeuAla: 8.807 ± 0.774
0.985LeuCys: 0.985 ± 0.248
6.651LeuAsp: 6.651 ± 0.795
5.235LeuGlu: 5.235 ± 0.544
1.54LeuPhe: 1.54 ± 0.345
5.85LeuGly: 5.85 ± 0.654
1.848LeuHis: 1.848 ± 0.32
2.956LeuIle: 2.956 ± 0.445
2.771LeuLys: 2.771 ± 0.482
3.88LeuLeu: 3.88 ± 0.527
2.155LeuMet: 2.155 ± 0.332
2.71LeuAsn: 2.71 ± 0.44
3.88LeuPro: 3.88 ± 0.605
3.202LeuGln: 3.202 ± 0.525
5.419LeuArg: 5.419 ± 0.607
4.804LeuSer: 4.804 ± 0.685
4.742LeuThr: 4.742 ± 0.517
5.481LeuVal: 5.481 ± 0.676
1.109LeuTrp: 1.109 ± 0.324
2.094LeuTyr: 2.094 ± 0.299
0.0LeuXaa: 0.0 ± 0.0
Met
3.88MetAla: 3.88 ± 0.572
0.554MetCys: 0.554 ± 0.154
1.601MetAsp: 1.601 ± 0.337
1.478MetGlu: 1.478 ± 0.233
0.616MetPhe: 0.616 ± 0.183
1.971MetGly: 1.971 ± 0.37
0.554MetHis: 0.554 ± 0.214
1.663MetIle: 1.663 ± 0.283
1.109MetLys: 1.109 ± 0.331
2.279MetLeu: 2.279 ± 0.44
0.616MetMet: 0.616 ± 0.222
0.985MetAsn: 0.985 ± 0.268
1.17MetPro: 1.17 ± 0.281
1.17MetGln: 1.17 ± 0.284
2.032MetArg: 2.032 ± 0.344
2.525MetSer: 2.525 ± 0.352
2.155MetThr: 2.155 ± 0.366
2.525MetVal: 2.525 ± 0.412
0.062MetTrp: 0.062 ± 0.057
0.37MetTyr: 0.37 ± 0.134
0.0MetXaa: 0.0 ± 0.0
Asn
4.065AsnAla: 4.065 ± 0.553
0.37AsnCys: 0.37 ± 0.183
2.155AsnAsp: 2.155 ± 0.326
1.17AsnGlu: 1.17 ± 0.259
1.232AsnPhe: 1.232 ± 0.294
4.249AsnGly: 4.249 ± 0.637
0.616AsnHis: 0.616 ± 0.175
1.478AsnIle: 1.478 ± 0.301
1.724AsnLys: 1.724 ± 0.312
2.525AsnLeu: 2.525 ± 0.336
1.232AsnMet: 1.232 ± 0.28
1.293AsnAsn: 1.293 ± 0.341
2.032AsnPro: 2.032 ± 0.323
1.601AsnGln: 1.601 ± 0.285
2.094AsnArg: 2.094 ± 0.397
2.34AsnSer: 2.34 ± 0.547
1.724AsnThr: 1.724 ± 0.31
2.402AsnVal: 2.402 ± 0.317
1.17AsnTrp: 1.17 ± 0.246
1.355AsnTyr: 1.355 ± 0.262
0.0AsnXaa: 0.0 ± 0.0
Pro
4.742ProAla: 4.742 ± 0.783
0.308ProCys: 0.308 ± 0.15
3.387ProAsp: 3.387 ± 0.478
3.941ProGlu: 3.941 ± 0.559
1.601ProPhe: 1.601 ± 0.279
3.633ProGly: 3.633 ± 0.52
0.924ProHis: 0.924 ± 0.275
2.279ProIle: 2.279 ± 0.359
3.079ProLys: 3.079 ± 0.469
2.833ProLeu: 2.833 ± 0.518
0.985ProMet: 0.985 ± 0.231
1.601ProAsn: 1.601 ± 0.381
1.663ProPro: 1.663 ± 0.341
0.924ProGln: 0.924 ± 0.238
2.402ProArg: 2.402 ± 0.453
2.525ProSer: 2.525 ± 0.402
2.155ProThr: 2.155 ± 0.377
4.065ProVal: 4.065 ± 0.495
0.554ProTrp: 0.554 ± 0.216
1.416ProTyr: 1.416 ± 0.373
0.0ProXaa: 0.0 ± 0.0
Gln
4.68GlnAla: 4.68 ± 0.512
0.801GlnCys: 0.801 ± 0.266
1.724GlnAsp: 1.724 ± 0.281
2.34GlnGlu: 2.34 ± 0.431
1.047GlnPhe: 1.047 ± 0.262
2.279GlnGly: 2.279 ± 0.391
1.109GlnHis: 1.109 ± 0.315
2.032GlnIle: 2.032 ± 0.383
2.34GlnLys: 2.34 ± 0.404
3.941GlnLeu: 3.941 ± 0.547
1.293GlnMet: 1.293 ± 0.31
1.232GlnAsn: 1.232 ± 0.303
1.663GlnPro: 1.663 ± 0.329
1.663GlnGln: 1.663 ± 0.379
2.402GlnArg: 2.402 ± 0.396
1.971GlnSer: 1.971 ± 0.404
1.971GlnThr: 1.971 ± 0.34
2.525GlnVal: 2.525 ± 0.489
0.616GlnTrp: 0.616 ± 0.241
1.232GlnTyr: 1.232 ± 0.274
0.0GlnXaa: 0.0 ± 0.0
Arg
6.282ArgAla: 6.282 ± 0.647
0.677ArgCys: 0.677 ± 0.184
4.065ArgAsp: 4.065 ± 0.539
3.941ArgGlu: 3.941 ± 0.467
1.971ArgPhe: 1.971 ± 0.351
4.065ArgGly: 4.065 ± 0.514
1.54ArgHis: 1.54 ± 0.377
4.434ArgIle: 4.434 ± 0.515
4.372ArgLys: 4.372 ± 0.627
5.974ArgLeu: 5.974 ± 0.713
1.971ArgMet: 1.971 ± 0.335
2.463ArgAsn: 2.463 ± 0.397
2.956ArgPro: 2.956 ± 0.518
3.141ArgGln: 3.141 ± 0.575
4.927ArgArg: 4.927 ± 0.692
3.757ArgSer: 3.757 ± 0.479
3.202ArgThr: 3.202 ± 0.454
3.326ArgVal: 3.326 ± 0.484
1.047ArgTrp: 1.047 ± 0.251
2.525ArgTyr: 2.525 ± 0.354
0.0ArgXaa: 0.0 ± 0.0
Ser
6.035SerAla: 6.035 ± 0.706
0.493SerCys: 0.493 ± 0.181
2.71SerAsp: 2.71 ± 0.378
3.079SerGlu: 3.079 ± 0.507
2.279SerPhe: 2.279 ± 0.398
5.235SerGly: 5.235 ± 0.649
1.17SerHis: 1.17 ± 0.218
3.387SerIle: 3.387 ± 0.452
2.525SerLys: 2.525 ± 0.424
4.804SerLeu: 4.804 ± 0.524
2.34SerMet: 2.34 ± 0.352
1.724SerAsn: 1.724 ± 0.264
2.463SerPro: 2.463 ± 0.408
1.663SerGln: 1.663 ± 0.272
3.141SerArg: 3.141 ± 0.422
2.34SerSer: 2.34 ± 0.362
2.648SerThr: 2.648 ± 0.524
3.141SerVal: 3.141 ± 0.405
1.416SerTrp: 1.416 ± 0.309
1.601SerTyr: 1.601 ± 0.29
0.0SerXaa: 0.0 ± 0.0
Thr
5.604ThrAla: 5.604 ± 0.592
0.739ThrCys: 0.739 ± 0.25
3.202ThrAsp: 3.202 ± 0.367
3.264ThrGlu: 3.264 ± 0.463
1.724ThrPhe: 1.724 ± 0.379
4.988ThrGly: 4.988 ± 0.591
0.677ThrHis: 0.677 ± 0.236
4.065ThrIle: 4.065 ± 0.598
2.463ThrLys: 2.463 ± 0.421
4.065ThrLeu: 4.065 ± 0.458
1.54ThrMet: 1.54 ± 0.284
2.279ThrAsn: 2.279 ± 0.473
3.018ThrPro: 3.018 ± 0.429
1.848ThrGln: 1.848 ± 0.398
2.956ThrArg: 2.956 ± 0.484
2.71ThrSer: 2.71 ± 0.465
3.695ThrThr: 3.695 ± 0.671
4.372ThrVal: 4.372 ± 0.688
0.677ThrTrp: 0.677 ± 0.242
2.894ThrTyr: 2.894 ± 0.48
0.0ThrXaa: 0.0 ± 0.0
Val
6.713ValAla: 6.713 ± 0.612
0.739ValCys: 0.739 ± 0.221
4.865ValAsp: 4.865 ± 0.575
5.296ValGlu: 5.296 ± 0.728
1.54ValPhe: 1.54 ± 0.341
5.173ValGly: 5.173 ± 0.621
0.739ValHis: 0.739 ± 0.263
4.003ValIle: 4.003 ± 0.54
3.818ValLys: 3.818 ± 0.464
4.988ValLeu: 4.988 ± 0.504
1.663ValMet: 1.663 ± 0.347
2.587ValAsn: 2.587 ± 0.397
2.833ValPro: 2.833 ± 0.451
2.279ValGln: 2.279 ± 0.387
5.235ValArg: 5.235 ± 0.534
3.941ValSer: 3.941 ± 0.435
4.188ValThr: 4.188 ± 0.481
4.311ValVal: 4.311 ± 0.596
1.232ValTrp: 1.232 ± 0.246
1.601ValTyr: 1.601 ± 0.306
0.0ValXaa: 0.0 ± 0.0
Trp
1.478TrpAla: 1.478 ± 0.36
0.062TrpCys: 0.062 ± 0.056
1.232TrpAsp: 1.232 ± 0.256
0.739TrpGlu: 0.739 ± 0.4
0.308TrpPhe: 0.308 ± 0.138
0.862TrpGly: 0.862 ± 0.235
0.554TrpHis: 0.554 ± 0.215
0.801TrpIle: 0.801 ± 0.189
0.554TrpLys: 0.554 ± 0.166
1.416TrpLeu: 1.416 ± 0.372
0.801TrpMet: 0.801 ± 0.246
0.493TrpAsn: 0.493 ± 0.188
0.862TrpPro: 0.862 ± 0.263
0.801TrpGln: 0.801 ± 0.238
1.355TrpArg: 1.355 ± 0.303
0.985TrpSer: 0.985 ± 0.229
0.616TrpThr: 0.616 ± 0.215
1.17TrpVal: 1.17 ± 0.346
0.185TrpTrp: 0.185 ± 0.105
0.246TrpTyr: 0.246 ± 0.111
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.894TyrAla: 2.894 ± 0.487
0.493TyrCys: 0.493 ± 0.203
1.909TyrAsp: 1.909 ± 0.363
1.663TyrGlu: 1.663 ± 0.321
0.801TyrPhe: 0.801 ± 0.26
2.71TyrGly: 2.71 ± 0.48
0.677TyrHis: 0.677 ± 0.254
0.985TyrIle: 0.985 ± 0.247
1.601TyrLys: 1.601 ± 0.295
1.786TyrLeu: 1.786 ± 0.423
0.554TyrMet: 0.554 ± 0.18
0.924TyrAsn: 0.924 ± 0.202
1.601TyrPro: 1.601 ± 0.338
1.663TyrGln: 1.663 ± 0.392
2.71TyrArg: 2.71 ± 0.364
1.909TyrSer: 1.909 ± 0.342
2.094TyrThr: 2.094 ± 0.394
1.909TyrVal: 1.909 ± 0.359
0.431TyrTrp: 0.431 ± 0.154
1.047TyrTyr: 1.047 ± 0.312
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (16239 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski