Fusobacterium necrophorum subsp. funduliforme B35

Taxonomy: cellular organisms; Bacteria; Fusobacteria; Fusobacteriia; Fusobacteriales; Fusobacteriaceae; Fusobacterium; Fusobacterium necrophorum; Fusobacterium necrophorum subsp. funduliforme

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1906 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A017H595|A0A017H595_9FUSO Isoleucine--tRNA ligase OS=Fusobacterium necrophorum subsp. funduliforme B35 OX=1226633 GN=ileS PE=3 SV=1
MM1 pKa = 7.36HH2 pKa = 7.27VIDD5 pKa = 5.5KK6 pKa = 7.44EE7 pKa = 4.55TCIGCGACEE16 pKa = 4.18GVCPVSTISATDD28 pKa = 3.42DD29 pKa = 3.16GKK31 pKa = 11.48YY32 pKa = 9.85EE33 pKa = 4.06VGDD36 pKa = 3.75ACVDD40 pKa = 4.0CGACAAGCPVSAISAQQ56 pKa = 3.28

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A017H4D1|A0A017H4D1_9FUSO Magnesium transport protein CorA OS=Fusobacterium necrophorum subsp. funduliforme B35 OX=1226633 GN=corA PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.56RR3 pKa = 11.84TFQPNTRR10 pKa = 11.84KK11 pKa = 9.88RR12 pKa = 11.84KK13 pKa = 8.28KK14 pKa = 9.85DD15 pKa = 2.89HH16 pKa = 6.16GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.49NGRR28 pKa = 11.84KK29 pKa = 7.92VLKK32 pKa = 9.96RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84QVLSAA44 pKa = 4.26

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1906

0

1906

629983

22

5688

330.5

37.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.19 ± 0.069

0.958 ± 0.023

4.493 ± 0.046

8.664 ± 0.072

4.868 ± 0.064

6.762 ± 0.073

1.761 ± 0.024

8.411 ± 0.064

8.824 ± 0.068

9.755 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.704 ± 0.033

4.571 ± 0.063

2.814 ± 0.039

3.292 ± 0.033

4.061 ± 0.039

6.157 ± 0.047

4.562 ± 0.037

6.23 ± 0.057

0.78 ± 0.018

4.145 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski