Kutzneria sp. 744

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Kutzneria; unclassified Kutzneria

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10148 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W7T443|W7T443_9PSEU Uncharacterized protein OS=Kutzneria sp. 744 OX=345341 GN=KUTG_08397 PE=4 SV=1
MM1 pKa = 7.27ATGISLLDD9 pKa = 4.31FIMEE13 pKa = 4.51LLGDD17 pKa = 3.76QSARR21 pKa = 11.84DD22 pKa = 3.75HH23 pKa = 6.9FEE25 pKa = 4.55SNPQQVLNDD34 pKa = 3.47HH35 pKa = 6.59GFNHH39 pKa = 6.86LCAADD44 pKa = 3.55VHH46 pKa = 6.81DD47 pKa = 5.69AMPLVIDD54 pKa = 4.61AAQSFDD60 pKa = 3.14RR61 pKa = 11.84TYY63 pKa = 11.3DD64 pKa = 3.02AGGPSHH70 pKa = 7.02VVTPPPPPPVHH81 pKa = 6.53GGGGMEE87 pKa = 4.5HH88 pKa = 7.49AIEE91 pKa = 4.15QIRR94 pKa = 11.84YY95 pKa = 6.37ITQNYY100 pKa = 8.7SYY102 pKa = 11.2SVDD105 pKa = 3.12SHH107 pKa = 5.48NTVVDD112 pKa = 3.33NSVNQNVWARR122 pKa = 11.84DD123 pKa = 3.53VFQSFDD129 pKa = 3.2NHH131 pKa = 6.09SVVTTGDD138 pKa = 3.52HH139 pKa = 5.68NVVGNGNEE147 pKa = 4.12VQNGDD152 pKa = 3.4LTADD156 pKa = 3.11HH157 pKa = 6.9GGAVAVGGSATGSNDD172 pKa = 3.43DD173 pKa = 3.9NSTDD177 pKa = 3.1VHH179 pKa = 7.59AYY181 pKa = 10.31GSGDD185 pKa = 3.25VATATNGSSLSQTDD199 pKa = 3.2SHH201 pKa = 6.26NTTDD205 pKa = 3.29SHH207 pKa = 7.33NSTDD211 pKa = 3.41SHH213 pKa = 6.81NDD215 pKa = 2.76SHH217 pKa = 7.48NNTAVGSGNDD227 pKa = 3.38SHH229 pKa = 7.57DD230 pKa = 3.58NTAVGSGNDD239 pKa = 3.37SHH241 pKa = 7.56DD242 pKa = 3.39NTAIGSGNDD251 pKa = 3.32SHH253 pKa = 7.9DD254 pKa = 4.28DD255 pKa = 3.54SHH257 pKa = 8.23DD258 pKa = 3.57NTAVGSGNDD267 pKa = 3.38SHH269 pKa = 7.58DD270 pKa = 3.57NTAVDD275 pKa = 4.35SGNDD279 pKa = 3.42SHH281 pKa = 7.78DD282 pKa = 4.37DD283 pKa = 3.41SHH285 pKa = 9.28DD286 pKa = 3.88DD287 pKa = 3.38VNTAIGSGNDD297 pKa = 3.17SHH299 pKa = 9.27DD300 pKa = 5.94DD301 pKa = 3.29IASDD305 pKa = 3.55NNTAIGSGNDD315 pKa = 3.34SHH317 pKa = 7.18DD318 pKa = 3.7HH319 pKa = 6.34SLTEE323 pKa = 4.44LDD325 pKa = 3.7SHH327 pKa = 7.1NDD329 pKa = 2.86TDD331 pKa = 3.88VASNNDD337 pKa = 2.88IASNNEE343 pKa = 3.23VDD345 pKa = 4.27HH346 pKa = 6.46NLIHH350 pKa = 7.02II351 pKa = 4.72

Molecular weight: 36.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W7T212|W7T212_9PSEU Uncharacterized protein OS=Kutzneria sp. 744 OX=345341 GN=KUTG_04410 PE=4 SV=1
MM1 pKa = 6.62NTAAAPIQTGTGALGGGSGGGSTGGGTGGGTRR33 pKa = 11.84VAAARR38 pKa = 11.84RR39 pKa = 11.84AGPGAPAVRR48 pKa = 11.84PAAAPRR54 pKa = 11.84AAPARR59 pKa = 11.84AAAA62 pKa = 4.2

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10148

0

10148

3322608

37

5422

327.4

35.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.896 ± 0.034

0.858 ± 0.008

6.169 ± 0.02

4.964 ± 0.02

2.904 ± 0.013

9.057 ± 0.027

2.356 ± 0.012

3.509 ± 0.016

2.023 ± 0.015

10.359 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.813 ± 0.009

2.19 ± 0.017

5.809 ± 0.021

3.122 ± 0.015

7.658 ± 0.031

5.432 ± 0.021

6.248 ± 0.022

8.938 ± 0.026

1.621 ± 0.01

2.074 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski