Staphylococcus phage 3MRA

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; Phietavirus; Staphylococcus virus 3MRA

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H3U2V4|A0A0H3U2V4_9CAUD Uncharacterized protein OS=Staphylococcus phage 3MRA OX=1505026 PE=4 SV=1
MM1 pKa = 7.45ATQKK5 pKa = 10.54QVDD8 pKa = 4.13YY9 pKa = 11.64VMSLQEE15 pKa = 3.89QLGLEE20 pKa = 4.28DD21 pKa = 3.85CEE23 pKa = 5.23KK24 pKa = 10.6YY25 pKa = 9.42TDD27 pKa = 3.83EE28 pKa = 4.01QVKK31 pKa = 10.4AMSHH35 pKa = 6.03KK36 pKa = 10.06EE37 pKa = 3.72VSNVIEE43 pKa = 4.26NYY45 pKa = 7.91KK46 pKa = 9.56TSIWDD51 pKa = 3.31EE52 pKa = 3.94EE53 pKa = 4.79LYY55 pKa = 10.65NEE57 pKa = 4.54CMSFGLPNCC66 pKa = 4.51

Molecular weight: 7.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H3U4W2|A0A0H3U4W2_9CAUD Uncharacterized protein OS=Staphylococcus phage 3MRA OX=1505026 PE=4 SV=1
MM1 pKa = 7.06NVAMLANPAIDD12 pKa = 3.42HH13 pKa = 6.54AVVKK17 pKa = 9.88YY18 pKa = 10.11RR19 pKa = 11.84NGMSTIAKK27 pKa = 6.81TVKK30 pKa = 10.38HH31 pKa = 4.96NTANKK36 pKa = 9.86

Molecular weight: 3.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

12391

36

956

187.7

21.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.786 ± 0.416

0.5 ± 0.102

6.133 ± 0.286

7.602 ± 0.464

4.221 ± 0.194

5.843 ± 0.376

1.84 ± 0.133

7.61 ± 0.263

8.805 ± 0.42

7.643 ± 0.302

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.825 ± 0.201

6.4 ± 0.307

2.946 ± 0.239

4.164 ± 0.236

4.1 ± 0.27

5.883 ± 0.264

6.02 ± 0.283

6.029 ± 0.299

1.202 ± 0.157

4.447 ± 0.387
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski