Amino acid dipepetide frequency for Streptococcus phage P3681

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.138AlaAla: 3.138 ± 1.016
0.179AlaCys: 0.179 ± 0.135
4.393AlaAsp: 4.393 ± 0.592
3.049AlaGlu: 3.049 ± 0.501
2.331AlaPhe: 2.331 ± 0.642
4.393AlaGly: 4.393 ± 0.875
1.166AlaHis: 1.166 ± 0.366
5.29AlaIle: 5.29 ± 0.811
6.725AlaLys: 6.725 ± 1.082
6.366AlaLeu: 6.366 ± 0.86
1.614AlaMet: 1.614 ± 0.449
4.035AlaAsn: 4.035 ± 0.851
1.793AlaPro: 1.793 ± 0.44
2.6AlaGln: 2.6 ± 0.582
2.421AlaArg: 2.421 ± 0.45
4.124AlaSer: 4.124 ± 0.62
4.304AlaThr: 4.304 ± 0.835
3.676AlaVal: 3.676 ± 0.743
0.897AlaTrp: 0.897 ± 0.222
2.511AlaTyr: 2.511 ± 0.501
0.0AlaXaa: 0.0 ± 0.0
Cys
0.179CysAla: 0.179 ± 0.116
0.0CysCys: 0.0 ± 0.0
0.628CysAsp: 0.628 ± 0.265
0.359CysGlu: 0.359 ± 0.159
0.179CysPhe: 0.179 ± 0.215
0.269CysGly: 0.269 ± 0.161
0.09CysHis: 0.09 ± 0.089
0.269CysIle: 0.269 ± 0.138
0.448CysLys: 0.448 ± 0.245
0.359CysLeu: 0.359 ± 0.196
0.09CysMet: 0.09 ± 0.107
0.628CysAsn: 0.628 ± 0.399
0.448CysPro: 0.448 ± 0.244
0.179CysGln: 0.179 ± 0.139
0.538CysArg: 0.538 ± 0.345
0.269CysSer: 0.269 ± 0.139
0.269CysThr: 0.269 ± 0.182
0.179CysVal: 0.179 ± 0.098
0.179CysTrp: 0.179 ± 0.135
0.269CysTyr: 0.269 ± 0.14
0.0CysXaa: 0.0 ± 0.0
Asp
3.497AspAla: 3.497 ± 0.719
0.359AspCys: 0.359 ± 0.179
4.752AspAsp: 4.752 ± 0.669
4.214AspGlu: 4.214 ± 0.738
3.317AspPhe: 3.317 ± 0.595
6.187AspGly: 6.187 ± 1.039
1.076AspHis: 1.076 ± 0.249
4.573AspIle: 4.573 ± 0.708
4.124AspLys: 4.124 ± 0.508
4.124AspLeu: 4.124 ± 0.737
2.152AspMet: 2.152 ± 0.441
3.945AspAsn: 3.945 ± 0.865
2.152AspPro: 2.152 ± 0.466
1.793AspGln: 1.793 ± 0.349
2.78AspArg: 2.78 ± 0.451
3.676AspSer: 3.676 ± 0.473
4.304AspThr: 4.304 ± 0.643
4.124AspVal: 4.124 ± 0.94
0.986AspTrp: 0.986 ± 0.233
2.69AspTyr: 2.69 ± 0.601
0.0AspXaa: 0.0 ± 0.0
Glu
3.586GluAla: 3.586 ± 0.546
0.359GluCys: 0.359 ± 0.158
3.497GluAsp: 3.497 ± 0.788
3.766GluGlu: 3.766 ± 0.648
2.78GluPhe: 2.78 ± 0.564
2.69GluGly: 2.69 ± 0.399
1.255GluHis: 1.255 ± 0.337
5.918GluIle: 5.918 ± 0.917
3.855GluLys: 3.855 ± 1.033
6.814GluLeu: 6.814 ± 0.838
1.793GluMet: 1.793 ± 0.477
4.214GluAsn: 4.214 ± 0.579
1.793GluPro: 1.793 ± 0.6
3.228GluGln: 3.228 ± 0.681
3.228GluArg: 3.228 ± 0.619
3.407GluSer: 3.407 ± 0.513
3.228GluThr: 3.228 ± 0.492
4.304GluVal: 4.304 ± 0.639
0.897GluTrp: 0.897 ± 0.252
3.317GluTyr: 3.317 ± 0.789
0.0GluXaa: 0.0 ± 0.0
Phe
3.049PheAla: 3.049 ± 0.521
0.09PheCys: 0.09 ± 0.102
3.317PheAsp: 3.317 ± 0.601
1.345PheGlu: 1.345 ± 0.363
1.435PhePhe: 1.435 ± 0.32
2.959PheGly: 2.959 ± 0.619
0.269PheHis: 0.269 ± 0.182
2.242PheIle: 2.242 ± 0.582
3.676PheLys: 3.676 ± 0.632
2.869PheLeu: 2.869 ± 0.496
0.717PheMet: 0.717 ± 0.267
3.676PheAsn: 3.676 ± 0.706
0.359PhePro: 0.359 ± 0.185
1.345PheGln: 1.345 ± 0.32
1.524PheArg: 1.524 ± 0.331
3.049PheSer: 3.049 ± 0.606
2.152PheThr: 2.152 ± 0.644
3.138PheVal: 3.138 ± 0.589
0.359PheTrp: 0.359 ± 0.155
1.883PheTyr: 1.883 ± 0.387
0.0PheXaa: 0.0 ± 0.0
Gly
3.945GlyAla: 3.945 ± 0.796
0.359GlyCys: 0.359 ± 0.215
4.124GlyAsp: 4.124 ± 0.692
3.586GlyGlu: 3.586 ± 0.633
3.049GlyPhe: 3.049 ± 0.495
4.393GlyGly: 4.393 ± 0.93
0.807GlyHis: 0.807 ± 0.247
4.931GlyIle: 4.931 ± 0.777
6.635GlyLys: 6.635 ± 0.893
6.276GlyLeu: 6.276 ± 0.92
1.614GlyMet: 1.614 ± 0.422
4.035GlyAsn: 4.035 ± 0.698
1.524GlyPro: 1.524 ± 0.523
2.869GlyGln: 2.869 ± 0.599
3.407GlyArg: 3.407 ± 0.591
4.662GlySer: 4.662 ± 0.879
4.124GlyThr: 4.124 ± 0.593
2.78GlyVal: 2.78 ± 0.526
1.435GlyTrp: 1.435 ± 0.331
3.138GlyTyr: 3.138 ± 0.549
0.0GlyXaa: 0.0 ± 0.0
His
0.448HisAla: 0.448 ± 0.19
0.0HisCys: 0.0 ± 0.0
0.986HisAsp: 0.986 ± 0.27
0.628HisGlu: 0.628 ± 0.18
0.717HisPhe: 0.717 ± 0.209
0.717HisGly: 0.717 ± 0.245
0.448HisHis: 0.448 ± 0.173
0.807HisIle: 0.807 ± 0.296
1.255HisLys: 1.255 ± 0.393
0.897HisLeu: 0.897 ± 0.264
0.359HisMet: 0.359 ± 0.163
0.897HisAsn: 0.897 ± 0.349
0.717HisPro: 0.717 ± 0.274
0.628HisGln: 0.628 ± 0.311
0.538HisArg: 0.538 ± 0.188
1.076HisSer: 1.076 ± 0.289
0.448HisThr: 0.448 ± 0.17
1.614HisVal: 1.614 ± 0.385
0.179HisTrp: 0.179 ± 0.132
0.897HisTyr: 0.897 ± 0.358
0.0HisXaa: 0.0 ± 0.0
Ile
4.842IleAla: 4.842 ± 0.72
0.359IleCys: 0.359 ± 0.194
5.111IleAsp: 5.111 ± 0.54
4.752IleGlu: 4.752 ± 0.855
1.255IlePhe: 1.255 ± 0.33
4.842IleGly: 4.842 ± 0.641
0.897IleHis: 0.897 ± 0.214
3.228IleIle: 3.228 ± 0.559
6.814IleLys: 6.814 ± 1.068
4.214IleLeu: 4.214 ± 0.782
1.973IleMet: 1.973 ± 0.499
3.945IleAsn: 3.945 ± 0.494
3.228IlePro: 3.228 ± 0.556
2.869IleGln: 2.869 ± 0.447
3.049IleArg: 3.049 ± 0.506
4.214IleSer: 4.214 ± 0.681
4.483IleThr: 4.483 ± 0.641
3.317IleVal: 3.317 ± 0.637
1.255IleTrp: 1.255 ± 0.238
2.6IleTyr: 2.6 ± 0.496
0.0IleXaa: 0.0 ± 0.0
Lys
5.738LysAla: 5.738 ± 0.654
0.538LysCys: 0.538 ± 0.306
4.124LysAsp: 4.124 ± 0.675
7.621LysGlu: 7.621 ± 0.852
3.586LysPhe: 3.586 ± 0.791
5.738LysGly: 5.738 ± 0.736
1.076LysHis: 1.076 ± 0.366
5.111LysIle: 5.111 ± 0.886
7.711LysLys: 7.711 ± 1.293
6.366LysLeu: 6.366 ± 0.907
1.973LysMet: 1.973 ± 0.441
5.649LysAsn: 5.649 ± 0.824
2.78LysPro: 2.78 ± 0.402
3.586LysGln: 3.586 ± 0.566
3.676LysArg: 3.676 ± 0.571
4.304LysSer: 4.304 ± 0.669
6.097LysThr: 6.097 ± 0.817
4.662LysVal: 4.662 ± 0.671
0.986LysTrp: 0.986 ± 0.254
3.407LysTyr: 3.407 ± 0.593
0.0LysXaa: 0.0 ± 0.0
Leu
6.994LeuAla: 6.994 ± 0.837
0.628LeuCys: 0.628 ± 0.239
5.29LeuAsp: 5.29 ± 0.75
6.276LeuGlu: 6.276 ± 1.012
2.511LeuPhe: 2.511 ± 0.389
5.021LeuGly: 5.021 ± 1.005
0.717LeuHis: 0.717 ± 0.259
4.931LeuIle: 4.931 ± 0.591
6.904LeuLys: 6.904 ± 0.747
6.366LeuLeu: 6.366 ± 0.682
2.78LeuMet: 2.78 ± 0.522
5.828LeuAsn: 5.828 ± 0.649
3.407LeuPro: 3.407 ± 0.466
2.6LeuGln: 2.6 ± 0.539
3.317LeuArg: 3.317 ± 0.772
5.38LeuSer: 5.38 ± 0.688
6.187LeuThr: 6.187 ± 0.861
3.945LeuVal: 3.945 ± 0.497
0.897LeuTrp: 0.897 ± 0.312
1.973LeuTyr: 1.973 ± 0.352
0.0LeuXaa: 0.0 ± 0.0
Met
1.973MetAla: 1.973 ± 0.46
0.09MetCys: 0.09 ± 0.088
0.448MetAsp: 0.448 ± 0.192
1.704MetGlu: 1.704 ± 0.538
1.255MetPhe: 1.255 ± 0.332
0.717MetGly: 0.717 ± 0.237
0.269MetHis: 0.269 ± 0.187
1.883MetIle: 1.883 ± 0.529
2.869MetLys: 2.869 ± 0.663
1.973MetLeu: 1.973 ± 0.365
0.359MetMet: 0.359 ± 0.167
0.986MetAsn: 0.986 ± 0.315
1.166MetPro: 1.166 ± 0.286
0.717MetGln: 0.717 ± 0.214
0.807MetArg: 0.807 ± 0.244
1.883MetSer: 1.883 ± 0.402
1.614MetThr: 1.614 ± 0.382
1.973MetVal: 1.973 ± 0.469
0.179MetTrp: 0.179 ± 0.108
0.628MetTyr: 0.628 ± 0.289
0.0MetXaa: 0.0 ± 0.0
Asn
5.021AsnAla: 5.021 ± 1.094
0.359AsnCys: 0.359 ± 0.222
4.124AsnAsp: 4.124 ± 0.647
3.855AsnGlu: 3.855 ± 0.796
2.152AsnPhe: 2.152 ± 0.486
7.263AsnGly: 7.263 ± 1.386
1.166AsnHis: 1.166 ± 0.334
3.945AsnIle: 3.945 ± 0.743
4.124AsnLys: 4.124 ± 0.687
5.649AsnLeu: 5.649 ± 0.625
0.986AsnMet: 0.986 ± 0.319
4.573AsnAsn: 4.573 ± 0.728
2.959AsnPro: 2.959 ± 0.611
2.69AsnGln: 2.69 ± 0.436
2.421AsnArg: 2.421 ± 0.572
3.676AsnSer: 3.676 ± 0.636
3.138AsnThr: 3.138 ± 0.509
3.228AsnVal: 3.228 ± 0.383
1.166AsnTrp: 1.166 ± 0.306
2.331AsnTyr: 2.331 ± 0.489
0.0AsnXaa: 0.0 ± 0.0
Pro
1.524ProAla: 1.524 ± 0.332
0.0ProCys: 0.0 ± 0.0
1.704ProAsp: 1.704 ± 0.493
2.421ProGlu: 2.421 ± 0.484
1.255ProPhe: 1.255 ± 0.301
1.166ProGly: 1.166 ± 0.401
0.448ProHis: 0.448 ± 0.199
1.524ProIle: 1.524 ± 0.3
3.945ProLys: 3.945 ± 0.559
2.511ProLeu: 2.511 ± 0.486
0.179ProMet: 0.179 ± 0.137
2.869ProAsn: 2.869 ± 0.516
0.807ProPro: 0.807 ± 0.358
1.704ProGln: 1.704 ± 0.324
1.166ProArg: 1.166 ± 0.324
2.421ProSer: 2.421 ± 0.338
2.959ProThr: 2.959 ± 0.499
1.793ProVal: 1.793 ± 0.501
0.448ProTrp: 0.448 ± 0.182
0.897ProTyr: 0.897 ± 0.322
0.0ProXaa: 0.0 ± 0.0
Gln
3.497GlnAla: 3.497 ± 0.681
0.179GlnCys: 0.179 ± 0.111
2.242GlnAsp: 2.242 ± 0.388
2.869GlnGlu: 2.869 ± 0.606
1.793GlnPhe: 1.793 ± 0.471
3.855GlnGly: 3.855 ± 0.746
0.538GlnHis: 0.538 ± 0.205
2.421GlnIle: 2.421 ± 0.629
3.049GlnLys: 3.049 ± 0.467
3.138GlnLeu: 3.138 ± 0.441
0.986GlnMet: 0.986 ± 0.262
2.242GlnAsn: 2.242 ± 0.407
0.538GlnPro: 0.538 ± 0.218
2.511GlnGln: 2.511 ± 0.548
1.614GlnArg: 1.614 ± 0.314
2.421GlnSer: 2.421 ± 0.585
2.959GlnThr: 2.959 ± 0.395
1.704GlnVal: 1.704 ± 0.387
0.897GlnTrp: 0.897 ± 0.397
2.152GlnTyr: 2.152 ± 0.368
0.0GlnXaa: 0.0 ± 0.0
Arg
2.331ArgAla: 2.331 ± 0.345
0.359ArgCys: 0.359 ± 0.294
2.6ArgAsp: 2.6 ± 0.616
2.331ArgGlu: 2.331 ± 0.434
1.614ArgPhe: 1.614 ± 0.418
2.511ArgGly: 2.511 ± 0.507
0.986ArgHis: 0.986 ± 0.333
3.317ArgIle: 3.317 ± 0.617
3.138ArgLys: 3.138 ± 0.5
3.676ArgLeu: 3.676 ± 0.859
1.076ArgMet: 1.076 ± 0.344
2.959ArgAsn: 2.959 ± 0.397
1.255ArgPro: 1.255 ± 0.316
1.793ArgGln: 1.793 ± 0.432
1.166ArgArg: 1.166 ± 0.384
1.524ArgSer: 1.524 ± 0.302
2.242ArgThr: 2.242 ± 0.766
3.138ArgVal: 3.138 ± 0.479
1.166ArgTrp: 1.166 ± 0.299
2.242ArgTyr: 2.242 ± 0.53
0.0ArgXaa: 0.0 ± 0.0
Ser
2.6SerAla: 2.6 ± 0.39
0.628SerCys: 0.628 ± 0.219
4.662SerAsp: 4.662 ± 0.512
3.945SerGlu: 3.945 ± 0.586
2.959SerPhe: 2.959 ± 0.404
4.124SerGly: 4.124 ± 0.577
0.359SerHis: 0.359 ± 0.159
5.021SerIle: 5.021 ± 0.542
5.38SerLys: 5.38 ± 0.676
4.304SerLeu: 4.304 ± 0.509
1.793SerMet: 1.793 ± 0.409
4.214SerAsn: 4.214 ± 0.551
2.062SerPro: 2.062 ± 0.493
2.6SerGln: 2.6 ± 0.6
2.421SerArg: 2.421 ± 0.652
4.573SerSer: 4.573 ± 1.135
4.214SerThr: 4.214 ± 0.547
4.842SerVal: 4.842 ± 0.841
0.628SerTrp: 0.628 ± 0.265
1.704SerTyr: 1.704 ± 0.555
0.0SerXaa: 0.0 ± 0.0
Thr
4.752ThrAla: 4.752 ± 0.812
0.359ThrCys: 0.359 ± 0.177
3.766ThrAsp: 3.766 ± 0.62
3.407ThrGlu: 3.407 ± 0.522
2.78ThrPhe: 2.78 ± 0.574
3.676ThrGly: 3.676 ± 0.55
1.166ThrHis: 1.166 ± 0.268
4.662ThrIle: 4.662 ± 0.878
5.111ThrLys: 5.111 ± 0.582
6.904ThrLeu: 6.904 ± 0.761
1.166ThrMet: 1.166 ± 0.317
3.855ThrAsn: 3.855 ± 0.803
1.435ThrPro: 1.435 ± 0.449
2.959ThrGln: 2.959 ± 0.678
1.704ThrArg: 1.704 ± 0.293
4.124ThrSer: 4.124 ± 0.546
3.317ThrThr: 3.317 ± 0.59
4.393ThrVal: 4.393 ± 0.542
1.076ThrTrp: 1.076 ± 0.283
3.407ThrTyr: 3.407 ± 0.646
0.0ThrXaa: 0.0 ± 0.0
Val
4.483ValAla: 4.483 ± 0.928
0.179ValCys: 0.179 ± 0.123
5.649ValAsp: 5.649 ± 0.69
3.945ValGlu: 3.945 ± 0.68
1.793ValPhe: 1.793 ± 0.378
4.393ValGly: 4.393 ± 0.512
0.538ValHis: 0.538 ± 0.188
4.393ValIle: 4.393 ± 0.682
4.931ValLys: 4.931 ± 0.568
4.124ValLeu: 4.124 ± 0.691
0.986ValMet: 0.986 ± 0.261
3.676ValAsn: 3.676 ± 0.676
2.062ValPro: 2.062 ± 0.366
1.793ValGln: 1.793 ± 0.417
2.152ValArg: 2.152 ± 0.569
4.752ValSer: 4.752 ± 0.692
4.752ValThr: 4.752 ± 0.655
3.586ValVal: 3.586 ± 0.53
0.807ValTrp: 0.807 ± 0.265
1.524ValTyr: 1.524 ± 0.441
0.0ValXaa: 0.0 ± 0.0
Trp
0.448TrpAla: 0.448 ± 0.181
0.09TrpCys: 0.09 ± 0.089
1.076TrpAsp: 1.076 ± 0.425
0.897TrpGlu: 0.897 ± 0.266
0.807TrpPhe: 0.807 ± 0.255
0.538TrpGly: 0.538 ± 0.228
0.269TrpHis: 0.269 ± 0.146
0.538TrpIle: 0.538 ± 0.198
0.986TrpLys: 0.986 ± 0.415
1.255TrpLeu: 1.255 ± 0.376
0.179TrpMet: 0.179 ± 0.135
1.255TrpAsn: 1.255 ± 0.469
0.179TrpPro: 0.179 ± 0.118
0.628TrpGln: 0.628 ± 0.268
0.986TrpArg: 0.986 ± 0.237
1.614TrpSer: 1.614 ± 0.466
1.345TrpThr: 1.345 ± 0.585
1.345TrpVal: 1.345 ± 0.258
0.269TrpTrp: 0.269 ± 0.186
0.179TrpTyr: 0.179 ± 0.109
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.959TyrAla: 2.959 ± 0.545
0.717TyrCys: 0.717 ± 0.403
2.331TyrAsp: 2.331 ± 0.51
2.959TyrGlu: 2.959 ± 0.688
1.883TyrPhe: 1.883 ± 0.4
2.242TyrGly: 2.242 ± 0.525
0.717TyrHis: 0.717 ± 0.238
2.242TyrIle: 2.242 ± 0.526
3.049TyrLys: 3.049 ± 0.677
3.766TyrLeu: 3.766 ± 0.492
0.628TyrMet: 0.628 ± 0.279
1.345TyrAsn: 1.345 ± 0.318
1.076TyrPro: 1.076 ± 0.349
2.511TyrGln: 2.511 ± 0.447
2.511TyrArg: 2.511 ± 0.606
1.883TyrSer: 1.883 ± 0.508
1.883TyrThr: 1.883 ± 0.534
2.78TyrVal: 2.78 ± 0.522
0.179TyrTrp: 0.179 ± 0.114
1.883TyrTyr: 1.883 ± 0.536
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 45 proteins (11154 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski