Gyrovirus 10
Average proteome isoelectric point is 8.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U9N4H7|A0A2U9N4H7_9VIRU Apoptin OS=Gyrovirus 10 OX=2218660 GN=VP3 PE=3 SV=1
MM1 pKa = 7.27 AHH3 pKa = 7.45 WSPATPIKK11 pKa = 10.45 SPPITPPDD19 pKa = 3.15 KK20 pKa = 10.66 RR21 pKa = 11.84 NYY23 pKa = 6.71 VTKK26 pKa = 10.15 TINGINASNKK36 pKa = 8.36 FCNVGWDD43 pKa = 3.65 KK44 pKa = 11.25 LQNSPDD50 pKa = 3.5 WYY52 pKa = 8.75 RR53 pKa = 11.84 ANYY56 pKa = 9.24 NRR58 pKa = 11.84 TIATWLRR65 pKa = 11.84 DD66 pKa = 3.5 CKK68 pKa = 9.82 TAHH71 pKa = 6.76 SKK73 pKa = 9.81 ICSCSSFRR81 pKa = 11.84 NHH83 pKa = 5.41 WHH85 pKa = 5.23 QTDD88 pKa = 3.33 ATLTTDD94 pKa = 3.99 SSTQTDD100 pKa = 3.52 VDD102 pKa = 3.55 TSPIIRR108 pKa = 11.84 AGEE111 pKa = 3.75 RR112 pKa = 11.84 ARR114 pKa = 11.84 RR115 pKa = 11.84 KK116 pKa = 9.05 ILARR120 pKa = 11.84 EE121 pKa = 3.95 RR122 pKa = 11.84 RR123 pKa = 11.84 QATPGNRR130 pKa = 11.84 KK131 pKa = 9.21 KK132 pKa = 10.46 KK133 pKa = 9.79 HH134 pKa = 5.55 KK135 pKa = 9.32 TVTWDD140 pKa = 3.0 IGPEE144 pKa = 4.0 EE145 pKa = 4.74 SSTPSDD151 pKa = 3.53 EE152 pKa = 5.8 DD153 pKa = 3.72 DD154 pKa = 4.72 GEE156 pKa = 5.0 SIDD159 pKa = 5.83 AFDD162 pKa = 4.73 LTEE165 pKa = 6.02 DD166 pKa = 4.68 DD167 pKa = 5.34 DD168 pKa = 6.25 DD169 pKa = 4.55 SAFANVNFDD178 pKa = 4.23 LATDD182 pKa = 4.3 LDD184 pKa = 4.17 AALNAPSGTNTPARR198 pKa = 11.84 MWW200 pKa = 3.83
Molecular weight: 22.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.971
IPC2_protein 5.855
IPC_protein 5.919
Toseland 6.262
ProMoST 6.275
Dawson 6.224
Bjellqvist 6.198
Wikipedia 6.249
Rodwell 6.211
Grimsley 6.389
Solomon 6.224
Lehninger 6.224
Nozaki 6.491
DTASelect 6.693
Thurlkill 6.693
EMBOSS 6.664
Sillero 6.605
Patrickios 4.546
IPC_peptide 6.249
IPC2_peptide 6.547
IPC2.peptide.svr19 6.557
Protein with the highest isoelectric point:
>tr|A0A2U9N6R0|A0A2U9N6R0_9VIRU CA1 OS=Gyrovirus 10 OX=2218660 GN=VP1 PE=3 SV=1
MM1 pKa = 7.98 GYY3 pKa = 10.06 RR4 pKa = 11.84 SRR6 pKa = 11.84 GKK8 pKa = 9.6 FYY10 pKa = 9.87 TFRR13 pKa = 11.84 RR14 pKa = 11.84 GRR16 pKa = 11.84 WRR18 pKa = 11.84 KK19 pKa = 8.41 HH20 pKa = 3.91 RR21 pKa = 11.84 RR22 pKa = 11.84 FRR24 pKa = 11.84 PYY26 pKa = 9.25 RR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 FRR33 pKa = 11.84 FRR35 pKa = 11.84 KK36 pKa = 8.53 RR37 pKa = 11.84 KK38 pKa = 8.72 FRR40 pKa = 11.84 PRR42 pKa = 11.84 YY43 pKa = 7.73 GFRR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 FKK50 pKa = 9.75 RR51 pKa = 11.84 AFWNQHH57 pKa = 4.93 PGSYY61 pKa = 9.62 VVRR64 pKa = 11.84 LPNPQNTLNLMFQGIVWFPLPKK86 pKa = 10.37 YY87 pKa = 10.13 CINSTNTLKK96 pKa = 11.12 NIDD99 pKa = 3.82 VQNVITCRR107 pKa = 11.84 VGSIHH112 pKa = 7.44 ISLKK116 pKa = 10.19 QFLLAVMSLSGTTKK130 pKa = 10.74 LGGPSIGKK138 pKa = 9.82 LYY140 pKa = 11.22 YY141 pKa = 9.56 EE142 pKa = 5.04 AKK144 pKa = 10.43 DD145 pKa = 3.55 KK146 pKa = 11.25 LGQAGNYY153 pKa = 9.89 PYY155 pKa = 9.45 MEE157 pKa = 5.51 KK158 pKa = 10.01 IASQTQSASLPSQWWRR174 pKa = 11.84 WAIMFMYY181 pKa = 10.31 PPDD184 pKa = 3.99 NKK186 pKa = 10.9 LIDD189 pKa = 4.14 YY190 pKa = 9.28 FPAQPDD196 pKa = 3.79 PKK198 pKa = 10.52 DD199 pKa = 3.51 LQNILGGWSLFRR211 pKa = 11.84 HH212 pKa = 6.18 KK213 pKa = 9.2 KK214 pKa = 7.72 TKK216 pKa = 9.84 VAIIATSPQGNWSPVASLTSQDD238 pKa = 3.58 SYY240 pKa = 11.93 FMRR243 pKa = 11.84 HH244 pKa = 5.97 CSNVFAVTKK253 pKa = 10.83 GNFPLSGAVGTKK265 pKa = 9.48 TPTSFLPDD273 pKa = 3.04 EE274 pKa = 4.87 CEE276 pKa = 4.18 YY277 pKa = 10.6 PSRR280 pKa = 11.84 PPQAEE285 pKa = 3.88 QTNQTGIPGQCYY297 pKa = 8.21 TRR299 pKa = 11.84 QPQDD303 pKa = 3.03 GLVEE307 pKa = 4.16 TDD309 pKa = 2.69 CWYY312 pKa = 10.53 SQEE315 pKa = 4.3 TFPSFGTLSALGASWAFPPGQRR337 pKa = 11.84 SFSHH341 pKa = 6.63 GSVNHH346 pKa = 5.03 HH347 pKa = 7.26 TITGSGDD354 pKa = 3.32 PEE356 pKa = 4.07 GQRR359 pKa = 11.84 WRR361 pKa = 11.84 TLNPLTTFPITSSEE375 pKa = 3.95 QGSDD379 pKa = 3.42 YY380 pKa = 11.41 DD381 pKa = 3.96 RR382 pKa = 11.84 VIASIYY388 pKa = 9.37 LAQGSDD394 pKa = 2.75 QFLPYY399 pKa = 10.31 KK400 pKa = 10.66 YY401 pKa = 9.96 GTDD404 pKa = 3.13 QDD406 pKa = 3.86 QPNWRR411 pKa = 11.84 PQKK414 pKa = 9.3 FTQQMNVWARR424 pKa = 11.84 IGEE427 pKa = 4.14 FRR429 pKa = 11.84 AA430 pKa = 3.62
Molecular weight: 49.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.307
IPC2_protein 9.736
IPC_protein 10.292
Toseland 10.277
ProMoST 10.028
Dawson 10.482
Bjellqvist 10.204
Wikipedia 10.687
Rodwell 10.774
Grimsley 10.57
Solomon 10.526
Lehninger 10.482
Nozaki 10.292
DTASelect 10.189
Thurlkill 10.335
EMBOSS 10.687
Sillero 10.394
Patrickios 10.321
IPC_peptide 10.526
IPC2_peptide 9.151
IPC2.peptide.svr19 8.597
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
738
108
430
246.0
27.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.962 ± 1.434
1.491 ± 0.208
5.149 ± 2.154
2.981 ± 0.56
4.336 ± 1.64
6.504 ± 1.273
2.304 ± 0.398
4.878 ± 0.389
5.014 ± 0.531
6.233 ± 1.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.762 ± 0.321
4.743 ± 0.943
7.588 ± 0.44
5.556 ± 1.519
8.266 ± 0.51
8.672 ± 0.577
8.672 ± 1.622
3.794 ± 0.657
2.846 ± 0.432
3.252 ± 1.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here