Gammapapillomavirus 12
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D2ALT7|A0A2D2ALT7_9PAPI ^E4 (Fragment) OS=Gammapapillomavirus 12 OX=1513257 GN=^E4 PE=4 SV=1
MM1 pKa = 7.34 SGSIRR6 pKa = 11.84 RR7 pKa = 11.84 KK8 pKa = 9.18 RR9 pKa = 11.84 ASPTDD14 pKa = 3.76 LYY16 pKa = 10.49 KK17 pKa = 11.02 ACVQGADD24 pKa = 4.46 CISDD28 pKa = 3.46 VKK30 pKa = 11.31 NKK32 pKa = 10.16 FEE34 pKa = 4.03 QTTIADD40 pKa = 3.73 WLLKK44 pKa = 10.4 IFGSLVYY51 pKa = 10.34 FGNLGIGTGKK61 pKa = 10.78 GSGGSLGFRR70 pKa = 11.84 PLGEE74 pKa = 4.0 GVGAGKK80 pKa = 9.56 PITTGAIKK88 pKa = 9.89 PAVAVEE94 pKa = 3.94 TLPVDD99 pKa = 3.78 VFAIPSEE106 pKa = 3.95 ASAIVPLAEE115 pKa = 5.05 GGPDD119 pKa = 3.33 LNVFTTDD126 pKa = 3.32 GGPGLGAEE134 pKa = 4.46 EE135 pKa = 4.39 IEE137 pKa = 5.26 LYY139 pKa = 10.41 TITDD143 pKa = 3.35 QTVDD147 pKa = 3.22 GDD149 pKa = 4.7 SIGGNPNIVTNEE161 pKa = 3.83 RR162 pKa = 11.84 GVPAVLDD169 pKa = 3.71 VQPTPEE175 pKa = 4.65 RR176 pKa = 11.84 PPQVLYY182 pKa = 11.03 DD183 pKa = 3.64 PTIDD187 pKa = 2.95 ATAQIRR193 pKa = 11.84 IVTSDD198 pKa = 3.33 PTLTQDD204 pKa = 3.11 TNIFVDD210 pKa = 4.58 PYY212 pKa = 10.64 VGGEE216 pKa = 3.88 IVGTGSRR223 pKa = 11.84 FEE225 pKa = 4.89 EE226 pKa = 4.28 IPLDD230 pKa = 4.04 EE231 pKa = 4.34 FTLGQFEE238 pKa = 4.61 IEE240 pKa = 4.18 EE241 pKa = 4.46 SPITSTPTEE250 pKa = 3.99 RR251 pKa = 11.84 LDD253 pKa = 3.46 NAVNRR258 pKa = 11.84 VRR260 pKa = 11.84 GFYY263 pKa = 10.54 NRR265 pKa = 11.84 LIRR268 pKa = 11.84 QVPVTDD274 pKa = 4.15 PVFLQQPSRR283 pKa = 11.84 LVQFEE288 pKa = 4.47 YY289 pKa = 10.88 EE290 pKa = 4.43 NPAFDD295 pKa = 5.75 DD296 pKa = 4.2 DD297 pKa = 4.34 VSLTFEE303 pKa = 4.65 RR304 pKa = 11.84 DD305 pKa = 3.12 LAAVTAAPHH314 pKa = 6.63 EE315 pKa = 4.57 DD316 pKa = 3.18 FADD319 pKa = 3.38 IVKK322 pKa = 10.67 LSRR325 pKa = 11.84 PEE327 pKa = 3.94 LSEE330 pKa = 4.05 SVPGVVRR337 pKa = 11.84 VSRR340 pKa = 11.84 LGEE343 pKa = 4.12 TNTITTRR350 pKa = 11.84 SGTTIGQKK358 pKa = 7.9 VHH360 pKa = 6.95 FYY362 pKa = 11.02 YY363 pKa = 10.68 DD364 pKa = 2.71 ISTIQEE370 pKa = 3.87 AEE372 pKa = 4.13 NIEE375 pKa = 4.23 LQTLGEE381 pKa = 4.27 HH382 pKa = 6.45 TGTSTIVDD390 pKa = 5.02 DD391 pKa = 4.55 ILASTIVDD399 pKa = 3.66 PVNNADD405 pKa = 3.85 VGISEE410 pKa = 5.81 DD411 pKa = 3.63 NLLDD415 pKa = 3.62 TFAEE419 pKa = 4.49 DD420 pKa = 3.74 FNNAHH425 pKa = 6.9 LVLQAAGNEE434 pKa = 4.17 DD435 pKa = 4.04 EE436 pKa = 6.0 DD437 pKa = 4.02 IVSIPLLPPGAALKK451 pKa = 10.92 LFVADD456 pKa = 3.66 VGDD459 pKa = 4.17 GLFVSYY465 pKa = 8.44 PTDD468 pKa = 3.82 TIHH471 pKa = 8.08 DD472 pKa = 3.79 ITIQLPDD479 pKa = 4.38 GLPIGPSYY487 pKa = 10.94 YY488 pKa = 10.8 VGVDD492 pKa = 2.73 NDD494 pKa = 4.44 FYY496 pKa = 11.31 LHH498 pKa = 7.23 PSLIPKK504 pKa = 9.77 KK505 pKa = 9.65 KK506 pKa = 9.47 RR507 pKa = 11.84 RR508 pKa = 11.84 RR509 pKa = 11.84 LEE511 pKa = 3.84 YY512 pKa = 10.83 YY513 pKa = 10.02 FF514 pKa = 5.21
Molecular weight: 55.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.347
IPC2_protein 4.215
IPC_protein 4.202
Toseland 4.012
ProMoST 4.342
Dawson 4.177
Bjellqvist 4.329
Wikipedia 4.075
Rodwell 4.037
Grimsley 3.923
Solomon 4.177
Lehninger 4.126
Nozaki 4.279
DTASelect 4.495
Thurlkill 4.037
EMBOSS 4.088
Sillero 4.317
Patrickios 3.745
IPC_peptide 4.177
IPC2_peptide 4.304
IPC2.peptide.svr19 4.244
Protein with the highest isoelectric point:
>tr|A0A2D2ALT7|A0A2D2ALT7_9PAPI ^E4 (Fragment) OS=Gammapapillomavirus 12 OX=1513257 GN=^E4 PE=4 SV=1
SS1 pKa = 7.02 IKK3 pKa = 10.3 IKK5 pKa = 10.84 LFLPLCLASQGLFPSLRR22 pKa = 11.84 RR23 pKa = 11.84 SLSPPPTPHH32 pKa = 6.9 PHH34 pKa = 6.16 RR35 pKa = 11.84 KK36 pKa = 8.54 SLEE39 pKa = 4.08 RR40 pKa = 11.84 EE41 pKa = 4.12 GEE43 pKa = 4.09 KK44 pKa = 10.19 PRR46 pKa = 11.84 RR47 pKa = 11.84 TPTPNRR53 pKa = 11.84 PPRR56 pKa = 11.84 PTLNFDD62 pKa = 4.51 EE63 pKa = 6.06 DD64 pKa = 4.17 DD65 pKa = 4.15 EE66 pKa = 4.74 NNKK69 pKa = 10.02 EE70 pKa = 4.01 NLPPPEE76 pKa = 4.3 YY77 pKa = 10.59 EE78 pKa = 4.31 EE79 pKa = 4.26 EE80 pKa = 4.17 QQGAATTLHH89 pKa = 5.28 QLLRR93 pKa = 11.84 KK94 pKa = 9.48 WEE96 pKa = 4.05 VEE98 pKa = 3.4 LDD100 pKa = 3.42 RR101 pKa = 11.84 FRR103 pKa = 11.84 DD104 pKa = 3.85 TVIQDD109 pKa = 3.48 LDD111 pKa = 3.65 AFKK114 pKa = 10.88 KK115 pKa = 10.56 RR116 pKa = 11.84 LGIRR120 pKa = 11.84 HH121 pKa = 6.09
Molecular weight: 14.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.17
IPC2_protein 7.98
IPC_protein 8.229
Toseland 8.916
ProMoST 8.478
Dawson 8.975
Bjellqvist 8.843
Wikipedia 9.004
Rodwell 9.151
Grimsley 8.668
Solomon 9.37
Lehninger 9.37
Nozaki 9.151
DTASelect 8.77
Thurlkill 8.946
EMBOSS 9.238
Sillero 9.121
Patrickios 7.673
IPC_peptide 9.355
IPC2_peptide 7.644
IPC2.peptide.svr19 7.512
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2379
92
599
339.9
38.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.557 ± 0.612
2.522 ± 0.956
6.389 ± 0.67
6.81 ± 0.51
4.75 ± 0.47
5.591 ± 1.194
1.765 ± 0.31
5.002 ± 0.718
5.633 ± 0.921
9.332 ± 0.45
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.429 ± 0.47
5.212 ± 0.522
5.717 ± 1.012
3.993 ± 0.23
5.591 ± 0.678
6.726 ± 0.477
6.011 ± 0.692
6.053 ± 0.73
1.303 ± 0.293
3.615 ± 0.35
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here