Streptococcus satellite phage Javan635
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 17 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZXP6|A0A4D5ZXP6_9VIRU HTH cro/C1-type domain-containing protein OS=Streptococcus satellite phage Javan635 OX=2558798 GN=JavanS635_0016 PE=4 SV=1
MM1 pKa = 7.46 MKK3 pKa = 9.51 TLSDD7 pKa = 3.62 TPNTYY12 pKa = 9.07 TFTATLPNYY21 pKa = 8.91 EE22 pKa = 4.06 QAQRR26 pKa = 11.84 AGNALLGYY34 pKa = 9.26 MVGTYY39 pKa = 9.29 DD40 pKa = 4.53 QSVIDD45 pKa = 3.64 ITYY48 pKa = 10.26 SGNGDD53 pKa = 3.31 IEE55 pKa = 4.38 VEE57 pKa = 4.26 YY58 pKa = 10.51 TSDD61 pKa = 3.63 EE62 pKa = 4.13 EE63 pKa = 4.19 LTEE66 pKa = 4.11 SFEE69 pKa = 5.08 RR70 pKa = 11.84 IADD73 pKa = 4.1 SFNHH77 pKa = 6.25 TDD79 pKa = 3.53 DD80 pKa = 5.62 FEE82 pKa = 7.63 DD83 pKa = 4.13 YY84 pKa = 10.86 DD85 pKa = 4.94 CDD87 pKa = 5.67 FIAQVNEE94 pKa = 3.87 LEE96 pKa = 4.59 KK97 pKa = 10.93 EE98 pKa = 4.08 FEE100 pKa = 3.99 ADD102 pKa = 3.19 YY103 pKa = 9.07 DD104 pKa = 3.83 TDD106 pKa = 3.96 TFKK109 pKa = 11.24 LDD111 pKa = 3.53 YY112 pKa = 11.0 HH113 pKa = 6.44 NMRR116 pKa = 11.84 VSQLKK121 pKa = 10.22 EE122 pKa = 3.7 LDD124 pKa = 3.55 KK125 pKa = 11.11 DD126 pKa = 3.78 TLINDD131 pKa = 4.14 LVLYY135 pKa = 10.08 EE136 pKa = 5.67 LEE138 pKa = 4.75 LLDD141 pKa = 5.41 HH142 pKa = 7.1 ADD144 pKa = 3.96 KK145 pKa = 11.13 LLSDD149 pKa = 4.42 EE150 pKa = 4.59 PLPLNSLDD158 pKa = 4.93 GYY160 pKa = 10.62 DD161 pKa = 3.61 TMEE164 pKa = 4.65 LLGDD168 pKa = 4.08 DD169 pKa = 4.0 VLSLVKK175 pKa = 10.42 FLDD178 pKa = 3.37 IDD180 pKa = 3.76 NEE182 pKa = 3.93 FDD184 pKa = 4.54 GIHH187 pKa = 7.36 DD188 pKa = 3.95 YY189 pKa = 11.16 VINGG193 pKa = 3.48
Molecular weight: 22.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.712
IPC2_protein 3.656
IPC_protein 3.681
Toseland 3.452
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.617
Rodwell 3.503
Grimsley 3.363
Solomon 3.668
Lehninger 3.617
Nozaki 3.783
DTASelect 4.037
Thurlkill 3.503
EMBOSS 3.617
Sillero 3.795
Patrickios 0.871
IPC_peptide 3.656
IPC2_peptide 3.77
IPC2.peptide.svr19 3.731
Protein with the highest isoelectric point:
>tr|A0A4D5ZSW7|A0A4D5ZSW7_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan635 OX=2558798 GN=JavanS635_0015 PE=4 SV=1
MM1 pKa = 7.83 KK2 pKa = 10.07 IIEE5 pKa = 4.27 YY6 pKa = 9.79 KK7 pKa = 10.74 KK8 pKa = 10.11 KK9 pKa = 10.53 DD10 pKa = 3.31 GTIMYY15 pKa = 9.41 RR16 pKa = 11.84 SQVYY20 pKa = 10.43 LGVDD24 pKa = 3.95 RR25 pKa = 11.84 ITGKK29 pKa = 10.47 KK30 pKa = 9.94 SRR32 pKa = 11.84 TSVSGRR38 pKa = 11.84 TKK40 pKa = 10.78 KK41 pKa = 9.89 EE42 pKa = 3.5 LKK44 pKa = 10.87 NNIKK48 pKa = 9.97 LAKK51 pKa = 10.26 FEE53 pKa = 4.23 FQANGEE59 pKa = 4.3 TLSKK63 pKa = 10.25 KK64 pKa = 9.62 VQVKK68 pKa = 7.02 TFKK71 pKa = 10.88 DD72 pKa = 3.81 LTDD75 pKa = 3.2 MWLVNYY81 pKa = 10.29 KK82 pKa = 8.93 LTLKK86 pKa = 9.06 PQSYY90 pKa = 10.34 NSTVSMLNKK99 pKa = 10.17 HH100 pKa = 5.7 ILPYY104 pKa = 9.19 FGKK107 pKa = 9.79 MKK109 pKa = 10.19 LDD111 pKa = 4.68 KK112 pKa = 9.82 ITSSDD117 pKa = 3.25 VQTFINSLSKK127 pKa = 10.05 KK128 pKa = 8.11 TINYY132 pKa = 8.86 VNARR136 pKa = 11.84 SIISRR141 pKa = 11.84 ILQHH145 pKa = 6.69 GVLLQLIQHH154 pKa = 6.08 NPARR158 pKa = 11.84 DD159 pKa = 3.15 IVLPRR164 pKa = 11.84 KK165 pKa = 8.88 QKK167 pKa = 10.58 KK168 pKa = 10.47 DD169 pKa = 2.97 SDD171 pKa = 3.74 KK172 pKa = 11.55 VKK174 pKa = 10.67 FIEE177 pKa = 5.41 KK178 pKa = 10.28 DD179 pKa = 3.47 DD180 pKa = 4.03 LKK182 pKa = 11.36 KK183 pKa = 10.77 FLDD186 pKa = 4.19 YY187 pKa = 10.73 IEE189 pKa = 4.82 KK190 pKa = 10.73 YY191 pKa = 10.29 KK192 pKa = 10.83 LRR194 pKa = 11.84 NYY196 pKa = 7.56 KK197 pKa = 9.94 TNFNYY202 pKa = 9.99 VLYY205 pKa = 10.41 KK206 pKa = 10.85 LLLATGLRR214 pKa = 11.84 IGEE217 pKa = 4.17 ACALEE222 pKa = 4.32 WSDD225 pKa = 3.79 IDD227 pKa = 5.93 LINGTINIDD236 pKa = 3.1 KK237 pKa = 9.64 TYY239 pKa = 10.23 SKK241 pKa = 10.54 SIRR244 pKa = 11.84 RR245 pKa = 11.84 LSTTKK250 pKa = 10.45 SKK252 pKa = 10.98 SGTRR256 pKa = 11.84 LISIDD261 pKa = 3.42 QATVNMLKK269 pKa = 10.42 LYY271 pKa = 8.44 QVRR274 pKa = 11.84 QRR276 pKa = 11.84 QLFHH280 pKa = 6.73 EE281 pKa = 4.87 VGGKK285 pKa = 9.36 APKK288 pKa = 10.35 VVFATPTRR296 pKa = 11.84 KK297 pKa = 9.63 YY298 pKa = 8.28 FHH300 pKa = 6.38 TFVRR304 pKa = 11.84 QNALDD309 pKa = 3.89 RR310 pKa = 11.84 ACIEE314 pKa = 3.8 AGIPRR319 pKa = 11.84 FTFHH323 pKa = 7.74 AFRR326 pKa = 11.84 HH327 pKa = 4.69 THH329 pKa = 6.92 ASLLLNAGISYY340 pKa = 10.68 KK341 pKa = 10.23 EE342 pKa = 3.61 LQYY345 pKa = 11.52 RR346 pKa = 11.84 LGHH349 pKa = 5.94 SNIAMTLDD357 pKa = 3.93 VYY359 pKa = 11.5 SHH361 pKa = 7.06 LSKK364 pKa = 11.04 DD365 pKa = 3.51 KK366 pKa = 10.41 EE367 pKa = 4.26 KK368 pKa = 10.88 EE369 pKa = 3.87 AVSYY373 pKa = 9.94 FEE375 pKa = 4.94 KK376 pKa = 10.6 AINSII381 pKa = 3.84
Molecular weight: 44.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.109
IPC2_protein 9.458
IPC_protein 9.385
Toseland 10.204
ProMoST 9.794
Dawson 10.365
Bjellqvist 9.984
Wikipedia 10.496
Rodwell 10.979
Grimsley 10.423
Solomon 10.379
Lehninger 10.35
Nozaki 10.175
DTASelect 9.984
Thurlkill 10.218
EMBOSS 10.599
Sillero 10.262
Patrickios 10.643
IPC_peptide 10.379
IPC2_peptide 8.463
IPC2.peptide.svr19 8.454
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
17
0
17
2266
40
381
133.3
15.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.854 ± 0.559
0.794 ± 0.169
6.178 ± 1.092
8.032 ± 0.932
4.81 ± 0.649
3.53 ± 0.515
1.721 ± 0.276
7.899 ± 0.887
10.018 ± 1.014
11.562 ± 0.758
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.383 ± 0.222
5.869 ± 0.519
2.339 ± 0.271
4.369 ± 0.506
3.795 ± 0.479
5.914 ± 0.524
5.737 ± 0.554
4.634 ± 0.429
0.75 ± 0.196
4.81 ± 0.308
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here