Mycobacterium phage Sparky

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Sparkyvirus; Mycobacterium virus Sparky

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A076GDR7|A0A076GDR7_9CAUD Lysin B OS=Mycobacterium phage Sparky OX=1527493 GN=27 PE=4 SV=1
MM1 pKa = 7.35LTTGGIQLSNPTNAEE16 pKa = 3.78DD17 pKa = 3.91YY18 pKa = 10.56EE19 pKa = 4.62FAFHH23 pKa = 7.12FSVKK27 pKa = 9.52GVKK30 pKa = 9.65LAGPGDD36 pKa = 4.17ADD38 pKa = 2.98TWYY41 pKa = 10.23PNIQYY46 pKa = 10.62GLRR49 pKa = 11.84GAGDD53 pKa = 4.03EE54 pKa = 4.23PPPLIVQAYY63 pKa = 9.66LMNAALNPDD72 pKa = 3.87PEE74 pKa = 4.5SVRR77 pKa = 11.84FSILYY82 pKa = 7.89TDD84 pKa = 3.97PVTWTEE90 pKa = 3.93VSPEE94 pKa = 4.09EE95 pKa = 4.29VDD97 pKa = 3.63ALPGSVEE104 pKa = 4.45DD105 pKa = 3.7YY106 pKa = 11.17SFAIMGDD113 pKa = 3.54STDD116 pKa = 3.41EE117 pKa = 4.0NGAQFRR123 pKa = 11.84LGLVGDD129 pKa = 4.31GAYY132 pKa = 10.36GLEE135 pKa = 4.12VSLFTLPIYY144 pKa = 10.73LDD146 pKa = 3.68SQDD149 pKa = 3.66QEE151 pKa = 4.68GAQLRR156 pKa = 11.84NVVLAHH162 pKa = 7.12APRR165 pKa = 11.84INWWAWEE172 pKa = 3.87MDD174 pKa = 3.32AA175 pKa = 6.11

Molecular weight: 19.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A076G982|A0A076G982_9CAUD Uncharacterized protein OS=Mycobacterium phage Sparky OX=1527493 GN=30 PE=4 SV=1
MM1 pKa = 7.05VLKK4 pKa = 9.89TRR6 pKa = 11.84LAHH9 pKa = 6.02LMRR12 pKa = 11.84RR13 pKa = 11.84AAHH16 pKa = 6.33ALIAAANKK24 pKa = 8.89LAPRR28 pKa = 11.84QAITITGIGAGGGGAGGTGNPRR50 pKa = 11.84PWIIAFF56 pKa = 3.88

Molecular weight: 5.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

91

0

91

20038

45

1797

220.2

24.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.154 ± 0.346

1.208 ± 0.155

6.762 ± 0.237

5.884 ± 0.246

2.84 ± 0.176

8.409 ± 0.485

2.6 ± 0.231

4.511 ± 0.182

3.184 ± 0.17

7.456 ± 0.208

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.046 ± 0.118

3.384 ± 0.201

6.208 ± 0.16

3.967 ± 0.252

7.421 ± 0.363

5.145 ± 0.205

6.313 ± 0.198

6.842 ± 0.206

2.176 ± 0.124

2.49 ± 0.121
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski