Acinetobacter phage vB_AbaM_B09_Aci02-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Saclayvirus; Acinetobacter virus Aci02-2

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 171 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A386KLA0|A0A386KLA0_9CAUD Putative carboxypeptidase OS=Acinetobacter phage vB_AbaM_B09_Aci02-2 OX=2315467 GN=Aci022_058 PE=4 SV=1
MM1 pKa = 7.71LYY3 pKa = 10.77SLVLVVFLSNGSEE16 pKa = 4.06DD17 pKa = 3.23RR18 pKa = 11.84TTLVKK23 pKa = 10.56NYY25 pKa = 9.73QDD27 pKa = 3.63YY28 pKa = 11.42SCIDD32 pKa = 3.07SRR34 pKa = 11.84DD35 pKa = 3.63VVRR38 pKa = 11.84SQLDD42 pKa = 3.55YY43 pKa = 11.67YY44 pKa = 11.7NNLEE48 pKa = 4.22SKK50 pKa = 11.09GDD52 pKa = 3.58VEE54 pKa = 5.21FVDD57 pKa = 5.21VEE59 pKa = 4.47CEE61 pKa = 3.86AQKK64 pKa = 11.33DD65 pKa = 4.17FII67 pKa = 5.94

Molecular weight: 7.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A386KKB6|A0A386KKB6_9CAUD Uncharacterized protein OS=Acinetobacter phage vB_AbaM_B09_Aci02-2 OX=2315467 GN=Aci022_024 PE=4 SV=1
MM1 pKa = 7.04YY2 pKa = 9.87LSRR5 pKa = 11.84VISSRR10 pKa = 11.84NMEE13 pKa = 4.66SITIQEE19 pKa = 4.08RR20 pKa = 11.84VKK22 pKa = 10.64RR23 pKa = 11.84CSLLGKK29 pKa = 9.58PMSLLQVYY37 pKa = 8.16FCKK40 pKa = 10.7FKK42 pKa = 10.92FVV44 pKa = 3.4

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

171

0

171

31108

37

2211

181.9

20.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.034 ± 0.292

1.321 ± 0.111

6.641 ± 0.181

7.31 ± 0.231

4.475 ± 0.178

6.677 ± 0.15

2.032 ± 0.145

6.555 ± 0.145

7.86 ± 0.282

8.053 ± 0.191

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.675 ± 0.141

5.179 ± 0.153

2.838 ± 0.117

3.478 ± 0.168

4.32 ± 0.16

6.426 ± 0.214

5.327 ± 0.221

7.104 ± 0.179

1.411 ± 0.077

4.285 ± 0.134
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski