Erethizon dorsatum papillomavirus 2
Average proteome isoelectric point is 5.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5ENG9|A0A2Z5ENG9_9PAPI Major capsid protein L1 OS=Erethizon dorsatum papillomavirus 2 OX=2268125 GN=L1 PE=3 SV=1
MM1 pKa = 6.95 VAVKK5 pKa = 10.35 RR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 LKK10 pKa = 10.33 RR11 pKa = 11.84 DD12 pKa = 3.04 SPEE15 pKa = 3.92 NIYY18 pKa = 10.15 RR19 pKa = 11.84 QCQISGNCPPDD30 pKa = 3.38 VKK32 pKa = 11.17 NKK34 pKa = 10.0 IEE36 pKa = 4.0 QNTLADD42 pKa = 5.95 RR43 pKa = 11.84 ILKK46 pKa = 8.59 WLSSIIYY53 pKa = 10.17 LGGLGIGTGRR63 pKa = 11.84 GTGGSRR69 pKa = 11.84 GYY71 pKa = 11.32 GPINPGGRR79 pKa = 11.84 VTGTGEE85 pKa = 4.22 VVHH88 pKa = 6.28 PAIPVDD94 pKa = 4.24 PIGPTDD100 pKa = 5.11 IIPVDD105 pKa = 4.1 PNSSSIVPLLEE116 pKa = 4.37 GGPTGDD122 pKa = 4.57 VPDD125 pKa = 4.7 ILPGPSGGEE134 pKa = 3.97 VTSGLDD140 pKa = 3.38 FASVDD145 pKa = 3.35 VTTSVEE151 pKa = 4.39 TIAEE155 pKa = 4.07 IAPTVDD161 pKa = 2.98 VTVQGAGEE169 pKa = 4.12 DD170 pKa = 3.4 TGATVVRR177 pKa = 11.84 PSTSRR182 pKa = 11.84 GATSTFFNPAYY193 pKa = 9.99 VSSIQPPAIAGEE205 pKa = 4.23 VGGEE209 pKa = 3.77 ISGLDD214 pKa = 3.46 VTDD217 pKa = 4.53 LSGFTAATFDD227 pKa = 3.56 RR228 pKa = 11.84 AGLQEE233 pKa = 4.89 EE234 pKa = 4.75 IEE236 pKa = 4.35 LMDD239 pKa = 3.69 IVDD242 pKa = 4.44 LEE244 pKa = 4.6 GEE246 pKa = 4.45 GVFDD250 pKa = 3.75 IFEE253 pKa = 4.67 PPSTSTPRR261 pKa = 11.84 NSLQRR266 pKa = 11.84 LIGRR270 pKa = 11.84 ARR272 pKa = 11.84 QLYY275 pKa = 8.74 NRR277 pKa = 11.84 RR278 pKa = 11.84 IRR280 pKa = 11.84 QQPVRR285 pKa = 11.84 NPLFIQRR292 pKa = 11.84 PQQLVTFEE300 pKa = 4.38 YY301 pKa = 10.32 EE302 pKa = 3.88 NPAFSDD308 pKa = 3.97 PEE310 pKa = 3.75 VTMIFEE316 pKa = 4.36 RR317 pKa = 11.84 DD318 pKa = 3.46 VAEE321 pKa = 4.21 VQIPPDD327 pKa = 3.87 FDD329 pKa = 4.1 FLDD332 pKa = 3.82 VARR335 pKa = 11.84 LGRR338 pKa = 11.84 PYY340 pKa = 10.81 LSEE343 pKa = 3.77 TADD346 pKa = 3.37 RR347 pKa = 11.84 TVRR350 pKa = 11.84 VSRR353 pKa = 11.84 LGQRR357 pKa = 11.84 SSLQTRR363 pKa = 11.84 QGTVVGEE370 pKa = 4.14 RR371 pKa = 11.84 VHH373 pKa = 6.88 YY374 pKa = 10.44 YY375 pKa = 10.91 YY376 pKa = 10.96 DD377 pKa = 4.32 LSPIAAVEE385 pKa = 4.17 SSSEE389 pKa = 4.23 SIEE392 pKa = 4.8 LNTLSAAPIQEE403 pKa = 4.22 GTVDD407 pKa = 3.38 TSIDD411 pKa = 3.55 NSSALLLDD419 pKa = 3.63 VFEE422 pKa = 5.45 EE423 pKa = 4.39 DD424 pKa = 3.85 FSNSRR429 pKa = 11.84 LHH431 pKa = 6.28 IPLYY435 pKa = 10.85 AGTRR439 pKa = 11.84 NYY441 pKa = 10.86 NVLEE445 pKa = 4.17 IPLVNRR451 pKa = 11.84 PSLPLLDD458 pKa = 5.84 DD459 pKa = 3.63 SGIWHH464 pKa = 7.49 LSGAPAGMAVQSSSEE479 pKa = 3.89 GDD481 pKa = 3.38 TTLVTVPFTSVDD493 pKa = 3.91 YY494 pKa = 10.27 YY495 pKa = 11.04 LHH497 pKa = 6.84 PSLVKK502 pKa = 9.46 RR503 pKa = 11.84 RR504 pKa = 11.84 RR505 pKa = 11.84 RR506 pKa = 11.84 RR507 pKa = 11.84 RR508 pKa = 11.84 RR509 pKa = 11.84 PFGDD513 pKa = 4.01 FFF515 pKa = 6.52
Molecular weight: 55.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.81
IPC2_protein 4.762
IPC_protein 4.711
Toseland 4.571
ProMoST 4.825
Dawson 4.673
Bjellqvist 4.813
Wikipedia 4.546
Rodwell 4.558
Grimsley 4.482
Solomon 4.673
Lehninger 4.622
Nozaki 4.774
DTASelect 4.952
Thurlkill 4.571
EMBOSS 4.571
Sillero 4.838
Patrickios 4.151
IPC_peptide 4.673
IPC2_peptide 4.825
IPC2.peptide.svr19 4.796
Protein with the highest isoelectric point:
>tr|A0A2Z5END3|A0A2Z5END3_9PAPI Protein E6 OS=Erethizon dorsatum papillomavirus 2 OX=2268125 GN=E6 PE=3 SV=1
MM1 pKa = 6.4 QTLEE5 pKa = 3.78 GRR7 pKa = 11.84 YY8 pKa = 9.71 NLLQEE13 pKa = 3.96 QLLNLFEE20 pKa = 5.24 KK21 pKa = 10.82 GSSEE25 pKa = 5.41 LEE27 pKa = 3.97 DD28 pKa = 3.93 QIQYY32 pKa = 10.22 WDD34 pKa = 3.88 LQRR37 pKa = 11.84 QEE39 pKa = 4.78 QILLHH44 pKa = 5.31 YY45 pKa = 10.03 ARR47 pKa = 11.84 KK48 pKa = 9.51 QGRR51 pKa = 11.84 SHH53 pKa = 7.91 IGMVTVPALSTSEE66 pKa = 4.14 YY67 pKa = 8.47 NAKK70 pKa = 10.18 VAIKK74 pKa = 10.58 VGLLLKK80 pKa = 10.6 SLAKK84 pKa = 9.87 SRR86 pKa = 11.84 YY87 pKa = 8.37 GRR89 pKa = 11.84 EE90 pKa = 3.79 PWSMQEE96 pKa = 3.95 TSSEE100 pKa = 4.13 LVLDD104 pKa = 4.47 TKK106 pKa = 10.99 PKK108 pKa = 10.96 GLFKK112 pKa = 10.99 KK113 pKa = 10.25 EE114 pKa = 3.88 GTHH117 pKa = 6.01 VDD119 pKa = 2.59 VWYY122 pKa = 10.18 DD123 pKa = 3.4 KK124 pKa = 11.25 DD125 pKa = 3.86 PEE127 pKa = 4.56 NGAQYY132 pKa = 10.87 VLWKK136 pKa = 10.39 YY137 pKa = 10.47 LYY139 pKa = 10.69 KK140 pKa = 10.08 DD141 pKa = 3.55 TEE143 pKa = 4.43 YY144 pKa = 11.39 GWVKK148 pKa = 10.54 LKK150 pKa = 10.92 SHH152 pKa = 6.25 VDD154 pKa = 3.17 YY155 pKa = 11.33 HH156 pKa = 6.43 GIYY159 pKa = 10.64 YY160 pKa = 10.17 KK161 pKa = 10.91 DD162 pKa = 3.39 EE163 pKa = 4.25 TDD165 pKa = 2.99 EE166 pKa = 4.63 KK167 pKa = 10.6 IYY169 pKa = 9.74 YY170 pKa = 9.82 TSFDD174 pKa = 3.81 EE175 pKa = 5.46 DD176 pKa = 3.59 GVRR179 pKa = 11.84 YY180 pKa = 8.22 STTGTWIVHH189 pKa = 5.87 FQNDD193 pKa = 4.46 TISSSRR199 pKa = 11.84 EE200 pKa = 3.37 EE201 pKa = 4.06 RR202 pKa = 11.84 DD203 pKa = 3.19 RR204 pKa = 11.84 SEE206 pKa = 4.28 TTGSAGTEE214 pKa = 4.06 TSRR217 pKa = 11.84 SEE219 pKa = 4.19 RR220 pKa = 11.84 PPPPRR225 pKa = 11.84 SRR227 pKa = 11.84 GSAGGGGGEE236 pKa = 3.74 RR237 pKa = 11.84 RR238 pKa = 11.84 RR239 pKa = 11.84 RR240 pKa = 11.84 SIEE243 pKa = 3.57 QAPNPTEE250 pKa = 3.7 VGQRR254 pKa = 11.84 RR255 pKa = 11.84 TSVPKK260 pKa = 10.63 HH261 pKa = 4.85 NLSRR265 pKa = 11.84 LKK267 pKa = 10.55 RR268 pKa = 11.84 LNLEE272 pKa = 4.04 ARR274 pKa = 11.84 DD275 pKa = 4.0 PPVLILKK282 pKa = 8.94 GQANALKK289 pKa = 10.06 CWRR292 pKa = 11.84 HH293 pKa = 4.12 RR294 pKa = 11.84 QKK296 pKa = 10.83 CKK298 pKa = 10.08 RR299 pKa = 11.84 EE300 pKa = 3.8 KK301 pKa = 10.85 LFVDD305 pKa = 4.14 ISTTFAWSGCGVGVGAGAGARR326 pKa = 11.84 KK327 pKa = 9.2 NRR329 pKa = 11.84 QINSRR334 pKa = 11.84 LLVAFADD341 pKa = 3.87 STHH344 pKa = 7.08 RR345 pKa = 11.84 DD346 pKa = 3.41 LFLKK350 pKa = 10.28 KK351 pKa = 9.98 IPIPKK356 pKa = 9.85 GCTYY360 pKa = 10.92 DD361 pKa = 3.56 YY362 pKa = 11.63 GNLSSLL368 pKa = 3.91
Molecular weight: 41.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.794
IPC2_protein 8.799
IPC_protein 8.712
Toseland 9.326
ProMoST 9.165
Dawson 9.648
Bjellqvist 9.385
Wikipedia 9.838
Rodwell 9.853
Grimsley 9.736
Solomon 9.677
Lehninger 9.633
Nozaki 9.443
DTASelect 9.355
Thurlkill 9.472
EMBOSS 9.78
Sillero 9.589
Patrickios 4.813
IPC_peptide 9.663
IPC2_peptide 7.995
IPC2.peptide.svr19 7.781
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2323
86
608
331.9
37.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.209 ± 0.48
2.109 ± 0.788
6.156 ± 0.351
6.457 ± 0.406
4.09 ± 0.589
6.974 ± 0.804
2.238 ± 0.386
4.649 ± 0.695
4.95 ± 1.025
9.643 ± 0.883
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.55 ± 0.403
4.133 ± 0.477
5.639 ± 1.008
5.08 ± 0.544
6.07 ± 0.77
7.232 ± 0.583
6.07 ± 0.521
6.802 ± 0.556
1.464 ± 0.31
3.487 ± 0.335
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here