Streptococcus satellite phage Javan731

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4D5ZU88|A0A4D5ZU88_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan731 OX=2558825 GN=JavanS731_0006 PE=4 SV=1
MM1 pKa = 7.17QVIVDD6 pKa = 3.83DD7 pKa = 3.73WCNRR11 pKa = 11.84GLNEE15 pKa = 4.17YY16 pKa = 7.72EE17 pKa = 3.77TGILYY22 pKa = 9.7FPKK25 pKa = 10.47HH26 pKa = 6.0EE27 pKa = 4.95DD28 pKa = 2.95KK29 pKa = 10.48MDD31 pKa = 4.04YY32 pKa = 10.61IRR34 pKa = 11.84FNKK37 pKa = 9.57GLEE40 pKa = 4.15PLYY43 pKa = 10.66HH44 pKa = 7.02ALDD47 pKa = 3.75EE48 pKa = 5.02PYY50 pKa = 8.07TTISKK55 pKa = 10.43SEE57 pKa = 3.88WLKK60 pKa = 11.29LLDD63 pKa = 3.66

Molecular weight:
7.6 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4D5ZZ37|A0A4D5ZZ37_9VIRU Transcriptional regulator OS=Streptococcus satellite phage Javan731 OX=2558825 GN=JavanS731_0005 PE=4 SV=1
MM1 pKa = 7.12MPKK4 pKa = 10.19SNYY7 pKa = 9.53RR8 pKa = 11.84EE9 pKa = 3.74MRR11 pKa = 11.84FISSLGEE18 pKa = 3.68KK19 pKa = 10.38LLATRR24 pKa = 11.84ILFTVTIANNQQ35 pKa = 3.18

Molecular weight:
4.07 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20

0

20

3056

35

507

152.8

17.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.726 ± 0.547

0.785 ± 0.195

5.432 ± 0.283

8.213 ± 0.587

4.123 ± 0.581

4.777 ± 0.453

1.898 ± 0.228

7.624 ± 0.394

9.62 ± 0.527

10.242 ± 0.628

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.421 ± 0.409

5.563 ± 0.344

3.207 ± 0.371

4.188 ± 0.373

4.908 ± 0.324

5.137 ± 0.393

5.694 ± 0.482

5.17 ± 0.425

0.884 ± 0.182

4.385 ± 0.347

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski