Ferroplasma acidarmanus fer1
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1927 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S0ALG4|S0ALG4_FERAC MFS domain-containing protein OS=Ferroplasma acidarmanus fer1 OX=333146 GN=FACI_IFERC00001G0203 PE=4 SV=1
MM1 pKa = 7.22 NVDD4 pKa = 4.49 DD5 pKa = 6.05 LFIYY9 pKa = 9.72 DD10 pKa = 4.49 LKK12 pKa = 11.0 YY13 pKa = 11.18 GKK15 pKa = 10.82 LNGQLINGEE24 pKa = 4.52 FYY26 pKa = 10.74 AIDD29 pKa = 4.05 GEE31 pKa = 4.58 YY32 pKa = 10.15 MNEE35 pKa = 4.03 DD36 pKa = 3.13 LPGILEE42 pKa = 4.13 RR43 pKa = 11.84 LL44 pKa = 3.63
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.781
IPC2_protein 3.961
IPC_protein 3.808
Toseland 3.617
ProMoST 3.999
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.77
Rodwell 3.656
Grimsley 3.541
Solomon 3.77
Lehninger 3.732
Nozaki 3.948
DTASelect 4.113
Thurlkill 3.706
EMBOSS 3.77
Sillero 3.935
Patrickios 1.901
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.838
Protein with the highest isoelectric point:
>tr|S0AQ34|S0AQ34_FERAC DNA-binding protein OS=Ferroplasma acidarmanus fer1 OX=333146 GN=FACI_IFERC00001G0299 PE=4 SV=1
MM1 pKa = 7.59 SKK3 pKa = 10.63 NKK5 pKa = 9.68 PLGKK9 pKa = 10.01 KK10 pKa = 8.82 IRR12 pKa = 11.84 LMKK15 pKa = 10.24 HH16 pKa = 4.57 VNSNRR21 pKa = 11.84 RR22 pKa = 11.84 VPGWVMMKK30 pKa = 9.4 TDD32 pKa = 4.22 RR33 pKa = 11.84 KK34 pKa = 8.14 MTQNPKK40 pKa = 8.8 RR41 pKa = 11.84 KK42 pKa = 8.26 NWRR45 pKa = 11.84 RR46 pKa = 11.84 SNLKK50 pKa = 10.1 LL51 pKa = 3.65
Molecular weight: 6.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 10.73
IPC_protein 12.237
Toseland 12.427
ProMoST 12.896
Dawson 12.427
Bjellqvist 12.413
Wikipedia 12.896
Rodwell 12.369
Grimsley 12.471
Solomon 12.91
Lehninger 12.808
Nozaki 12.427
DTASelect 12.413
Thurlkill 12.427
EMBOSS 12.925
Sillero 12.427
Patrickios 12.106
IPC_peptide 12.91
IPC2_peptide 11.886
IPC2.peptide.svr19 9.0
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1927
0
1927
560709
29
2061
291.0
32.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.929 ± 0.061
0.605 ± 0.017
5.412 ± 0.057
6.142 ± 0.066
4.746 ± 0.053
6.828 ± 0.053
1.616 ± 0.02
10.107 ± 0.06
7.476 ± 0.065
8.665 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.061 ± 0.028
6.057 ± 0.065
3.484 ± 0.034
1.907 ± 0.024
3.905 ± 0.041
7.294 ± 0.054
5.04 ± 0.052
6.006 ± 0.043
0.753 ± 0.017
4.967 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here