uncultured archaeon A07HR67

Taxonomy: cellular organisms; Archaea; environmental samples

Average proteome isoelectric point is 5.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2887 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V4XS26|V4XS26_9ARCH ATPase involved in chromosome partitioning OS=uncultured archaeon A07HR67 OX=1412871 GN=A07HR67_02377 PE=4 SV=1
MM1 pKa = 7.51AVLVIAIMASAAGNIMEE18 pKa = 4.35QTLGVPYY25 pKa = 10.17LAGVGAVIAVVGVLSYY41 pKa = 11.37VGDD44 pKa = 3.89SLIEE48 pKa = 3.94RR49 pKa = 11.84FKK51 pKa = 10.82TGGTVFLYY59 pKa = 10.55LAYY62 pKa = 9.11LTFGIVVLAATWGDD76 pKa = 3.26ITSVFAAGDD85 pKa = 3.3TSYY88 pKa = 11.83ASDD91 pKa = 3.9ASPLAVLRR99 pKa = 11.84SGILYY104 pKa = 9.9VGYY107 pKa = 10.55NLVVLPAVFFALHH120 pKa = 5.64RR121 pKa = 11.84QVDD124 pKa = 3.6RR125 pKa = 11.84RR126 pKa = 11.84EE127 pKa = 4.24SVVSGVLAGTLMTIPFAITYY147 pKa = 7.47VAFMGFYY154 pKa = 9.84PAEE157 pKa = 4.2NVMGAPVPWLPVLNGVGGPALIGLYY182 pKa = 9.76GVVMGWTLIEE192 pKa = 4.08TSVGLIHH199 pKa = 7.53AIISRR204 pKa = 11.84LDD206 pKa = 3.52TNIEE210 pKa = 4.14EE211 pKa = 4.16IDD213 pKa = 3.48AGPFEE218 pKa = 4.59TQTGLSRR225 pKa = 11.84TQRR228 pKa = 11.84GGLAVGVLVGALGLSRR244 pKa = 11.84VGIIDD249 pKa = 4.84LVAQGYY255 pKa = 5.76TTMAYY260 pKa = 9.92FFIGIFAIPLXXXXXXXPGQLFNFASMYY288 pKa = 10.4VPSNDD293 pKa = 3.16VFVSSAAGIEE303 pKa = 4.26LFNGEE308 pKa = 4.78DD309 pKa = 3.66PVSGEE314 pKa = 3.94VGEE317 pKa = 6.43DD318 pKa = 2.93IALWDD323 pKa = 4.44AGTEE327 pKa = 4.24RR328 pKa = 11.84NGQPGVDD335 pKa = 3.62PVSAPLQAQNGGPMAGPQEE354 pKa = 4.53GVVHH358 pKa = 6.99RR359 pKa = 11.84LDD361 pKa = 5.55DD362 pKa = 4.03INDD365 pKa = 3.68GFDD368 pKa = 3.24YY369 pKa = 11.28ADD371 pKa = 3.45ASEE374 pKa = 4.48IVDD377 pKa = 3.38VTLTHH382 pKa = 7.26DD383 pKa = 3.72GDD385 pKa = 3.97GSFTVRR391 pKa = 11.84LEE393 pKa = 4.1NTSPTDD399 pKa = 3.37VYY401 pKa = 11.5GDD403 pKa = 3.62DD404 pKa = 3.88TATGGQVWITPGAWAVHH421 pKa = 5.93TGSNPIFEE429 pKa = 4.85RR430 pKa = 11.84GEE432 pKa = 3.77PASIGLEE439 pKa = 3.91ALAEE443 pKa = 4.44AGPPTGFEE451 pKa = 4.15GQPGLVDD458 pKa = 4.23EE459 pKa = 5.58LPEE462 pKa = 5.43ADD464 pKa = 3.37NVVDD468 pKa = 3.9VGAYY472 pKa = 7.68TPSNTVTDD480 pKa = 4.59PNDD483 pKa = 3.24PMGAVPGAPPIAPGGAFEE501 pKa = 4.61FTVEE505 pKa = 4.0ADD507 pKa = 3.25PSEE510 pKa = 4.26RR511 pKa = 11.84LSFASMYY518 pKa = 10.48VPSNDD523 pKa = 2.84VFIAPEE529 pKa = 3.92PAIEE533 pKa = 5.19LYY535 pKa = 10.29TDD537 pKa = 3.61DD538 pKa = 4.82GEE540 pKa = 4.33PVEE543 pKa = 4.97GGVTEE548 pKa = 5.86SVGLWDD554 pKa = 5.93AGTEE558 pKa = 4.2LNGRR562 pKa = 11.84PGVDD566 pKa = 3.25PASAPLQAQNGGPTVGPQEE585 pKa = 4.46GVVHH589 pKa = 6.95RR590 pKa = 11.84LDD592 pKa = 6.01DD593 pKa = 3.97IDD595 pKa = 6.33DD596 pKa = 4.33GFDD599 pKa = 3.27YY600 pKa = 11.38ADD602 pKa = 3.47ASEE605 pKa = 4.39IVSVTIEE612 pKa = 3.96PQQ614 pKa = 2.82

Molecular weight: 62.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V4Y017|V4Y017_9ARCH ABC-type dipeptide/oligopeptide/nickel transport system permease component OS=uncultured archaeon A07HR67 OX=1412871 GN=A07HR67_01204 PE=3 SV=1
MM1 pKa = 7.48ARR3 pKa = 11.84FDD5 pKa = 3.4AAEE8 pKa = 3.94RR9 pKa = 11.84RR10 pKa = 11.84NLDD13 pKa = 2.96RR14 pKa = 11.84QICMRR19 pKa = 11.84CNARR23 pKa = 11.84NAPDD27 pKa = 3.69ADD29 pKa = 3.85RR30 pKa = 11.84CRR32 pKa = 11.84KK33 pKa = 9.62CGYY36 pKa = 9.02TNLRR40 pKa = 11.84PKK42 pKa = 10.52AKK44 pKa = 9.72EE45 pKa = 3.61RR46 pKa = 11.84RR47 pKa = 11.84ATT49 pKa = 3.4

Molecular weight: 5.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2887

0

2887

769015

23

1860

266.4

28.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.351 ± 0.088

0.701 ± 0.015

8.576 ± 0.064

7.803 ± 0.053

3.17 ± 0.031

8.895 ± 0.042

1.915 ± 0.023

3.706 ± 0.038

1.472 ± 0.025

8.464 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.658 ± 0.019

2.086 ± 0.025

4.956 ± 0.03

2.01 ± 0.025

7.295 ± 0.054

5.182 ± 0.037

6.583 ± 0.036

9.576 ± 0.053

1.04 ± 0.019

2.455 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski