Amino acid dipepetide frequency for Ideonella sakaiensis (strain NBRC 110686 / TISTR 2288 / 201-F6)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	26.762AlaAla: 26.762 ± 0.237	1.5AlaCys: 1.5 ± 0.033	7.775AlaAsp: 7.775 ± 0.071	7.921AlaGlu: 7.921 ± 0.077	4.079AlaPhe: 4.079 ± 0.05	14.302AlaGly: 14.302 ± 0.116	2.81AlaHis: 2.81 ± 0.042	4.465AlaIle: 4.465 ± 0.057	2.598AlaLys: 2.598 ± 0.051	18.128AlaLeu: 18.128 ± 0.143	3.321AlaMet: 3.321 ± 0.047	2.203AlaAsn: 2.203 ± 0.034	9.288AlaPro: 9.288 ± 0.116	5.51AlaGln: 5.51 ± 0.075	12.775AlaArg: 12.775 ± 0.111	6.616AlaSer: 6.616 ± 0.068	6.499AlaThr: 6.499 ± 0.059	10.314AlaVal: 10.314 ± 0.073	2.613AlaTrp: 2.613 ± 0.044	2.358AlaTyr: 2.358 ± 0.039	0.0AlaXaa: 0.0 ± 0.0
Cys	1.248CysAla: 1.248 ± 0.024	0.118CysCys: 0.118 ± 0.009	0.503CysAsp: 0.503 ± 0.018	0.513CysGlu: 0.513 ± 0.018	0.287CysPhe: 0.287 ± 0.011	0.99CysGly: 0.99 ± 0.027	0.253CysHis: 0.253 ± 0.013	0.325CysIle: 0.325 ± 0.013	0.131CysLys: 0.131 ± 0.008	0.815CysLeu: 0.815 ± 0.023	0.152CysMet: 0.152 ± 0.009	0.183CysAsn: 0.183 ± 0.011	0.528CysPro: 0.528 ± 0.018	0.205CysGln: 0.205 ± 0.011	0.828CysArg: 0.828 ± 0.022	0.479CysSer: 0.479 ± 0.017	0.427CysThr: 0.427 ± 0.015	0.668CysVal: 0.668 ± 0.021	0.115CysTrp: 0.115 ± 0.008	0.174CysTyr: 0.174 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	8.523AspAla: 8.523 ± 0.076	0.428AspCys: 0.428 ± 0.015	2.881AspAsp: 2.881 ± 0.044	3.49AspGlu: 3.49 ± 0.054	1.797AspPhe: 1.797 ± 0.033	5.833AspGly: 5.833 ± 0.073	1.122AspHis: 1.122 ± 0.026	1.736AspIle: 1.736 ± 0.037	1.111AspLys: 1.111 ± 0.03	5.39AspLeu: 5.39 ± 0.068	0.889AspMet: 0.889 ± 0.022	0.852AspAsn: 0.852 ± 0.021	3.814AspPro: 3.814 ± 0.045	1.33AspGln: 1.33 ± 0.028	4.151AspArg: 4.151 ± 0.054	1.702AspSer: 1.702 ± 0.029	2.405AspThr: 2.405 ± 0.036	3.607AspVal: 3.607 ± 0.045	1.015AspTrp: 1.015 ± 0.022	1.14AspTyr: 1.14 ± 0.026	0.0AspXaa: 0.0 ± 0.0
Glu	8.429GluAla: 8.429 ± 0.087	0.314GluCys: 0.314 ± 0.014	2.289GluAsp: 2.289 ± 0.037	2.152GluGlu: 2.152 ± 0.038	1.452GluPhe: 1.452 ± 0.028	3.808GluGly: 3.808 ± 0.041	1.317GluHis: 1.317 ± 0.029	2.148GluIle: 2.148 ± 0.039	1.14GluLys: 1.14 ± 0.032	5.702GluLeu: 5.702 ± 0.074	0.979GluMet: 0.979 ± 0.029	0.872GluAsn: 0.872 ± 0.023	3.017GluPro: 3.017 ± 0.047	2.235GluGln: 2.235 ± 0.039	5.688GluArg: 5.688 ± 0.071	1.866GluSer: 1.866 ± 0.034	2.201GluThr: 2.201 ± 0.042	4.237GluVal: 4.237 ± 0.056	0.619GluTrp: 0.619 ± 0.021	0.739GluTyr: 0.739 ± 0.023	0.0GluXaa: 0.0 ± 0.0
Phe	4.066PheAla: 4.066 ± 0.055	0.326PheCys: 0.326 ± 0.015	2.28PheAsp: 2.28 ± 0.036	1.886PheGlu: 1.886 ± 0.033	1.082PhePhe: 1.082 ± 0.029	3.177PheGly: 3.177 ± 0.045	0.696PheHis: 0.696 ± 0.02	1.012PheIle: 1.012 ± 0.026	0.739PheLys: 0.739 ± 0.02	2.652PheLeu: 2.652 ± 0.04	0.564PheMet: 0.564 ± 0.019	0.762PheAsn: 0.762 ± 0.02	1.386PhePro: 1.386 ± 0.029	0.927PheGln: 0.927 ± 0.024	2.062PheArg: 2.062 ± 0.036	1.606PheSer: 1.606 ± 0.031	1.512PheThr: 1.512 ± 0.031	2.655PheVal: 2.655 ± 0.044	0.459PheTrp: 0.459 ± 0.016	0.675PheTyr: 0.675 ± 0.019	0.0PheXaa: 0.0 ± 0.0
Gly	11.386GlyAla: 11.386 ± 0.089	0.985GlyCys: 0.985 ± 0.03	4.309GlyAsp: 4.309 ± 0.05	4.78GlyGlu: 4.78 ± 0.053	2.996GlyPhe: 2.996 ± 0.043	8.275GlyGly: 8.275 ± 0.102	2.072GlyHis: 2.072 ± 0.039	3.321GlyIle: 3.321 ± 0.042	2.116GlyLys: 2.116 ± 0.041	10.594GlyLeu: 10.594 ± 0.102	1.986GlyMet: 1.986 ± 0.032	1.638GlyAsn: 1.638 ± 0.038	4.837GlyPro: 4.837 ± 0.059	3.174GlyGln: 3.174 ± 0.039	8.271GlyArg: 8.271 ± 0.077	4.765GlySer: 4.765 ± 0.052	4.415GlyThr: 4.415 ± 0.058	6.59GlyVal: 6.59 ± 0.065	1.757GlyTrp: 1.757 ± 0.039	2.014GlyTyr: 2.014 ± 0.037	0.0GlyXaa: 0.0 ± 0.0
His	3.203HisAla: 3.203 ± 0.049	0.29HisCys: 0.29 ± 0.014	1.202HisAsp: 1.202 ± 0.029	1.146HisGlu: 1.146 ± 0.026	0.74HisPhe: 0.74 ± 0.021	2.4HisGly: 2.4 ± 0.04	0.609HisHis: 0.609 ± 0.02	0.586HisIle: 0.586 ± 0.018	0.353HisLys: 0.353 ± 0.014	2.332HisLeu: 2.332 ± 0.04	0.333HisMet: 0.333 ± 0.013	0.35HisAsn: 0.35 ± 0.013	1.655HisPro: 1.655 ± 0.032	0.634HisGln: 0.634 ± 0.019	1.897HisArg: 1.897 ± 0.04	0.713HisSer: 0.713 ± 0.02	0.858HisThr: 0.858 ± 0.024	1.673HisVal: 1.673 ± 0.028	0.449HisTrp: 0.449 ± 0.017	0.53HisTyr: 0.53 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	5.184IleAla: 5.184 ± 0.055	0.262IleCys: 0.262 ± 0.013	2.781IleAsp: 2.781 ± 0.04	2.453IleGlu: 2.453 ± 0.034	0.849IlePhe: 0.849 ± 0.024	3.639IleGly: 3.639 ± 0.053	0.66IleHis: 0.66 ± 0.018	0.842IleIle: 0.842 ± 0.026	0.861IleLys: 0.861 ± 0.027	2.374IleLeu: 2.374 ± 0.041	0.442IleMet: 0.442 ± 0.016	0.844IleAsn: 0.844 ± 0.024	1.571IlePro: 1.571 ± 0.029	0.869IleGln: 0.869 ± 0.024	2.233IleArg: 2.233 ± 0.034	1.508IleSer: 1.508 ± 0.031	1.753IleThr: 1.753 ± 0.031	3.053IleVal: 3.053 ± 0.046	0.298IleTrp: 0.298 ± 0.012	0.668IleTyr: 0.668 ± 0.022	0.0IleXaa: 0.0 ± 0.0
Lys	2.978LysAla: 2.978 ± 0.051	0.125LysCys: 0.125 ± 0.008	1.089LysAsp: 1.089 ± 0.028	0.949LysGlu: 0.949 ± 0.031	0.589LysPhe: 0.589 ± 0.02	1.684LysGly: 1.684 ± 0.037	0.398LysHis: 0.398 ± 0.015	0.824LysIle: 0.824 ± 0.026	0.765LysLys: 0.765 ± 0.026	2.338LysLeu: 2.338 ± 0.045	0.466LysMet: 0.466 ± 0.018	0.492LysAsn: 0.492 ± 0.018	1.397LysPro: 1.397 ± 0.029	0.762LysGln: 0.762 ± 0.024	1.601LysArg: 1.601 ± 0.03	1.042LysSer: 1.042 ± 0.022	1.186LysThr: 1.186 ± 0.025	1.739LysVal: 1.739 ± 0.036	0.233LysTrp: 0.233 ± 0.011	0.413LysTyr: 0.413 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	18.335LeuAla: 18.335 ± 0.145	0.92LeuCys: 0.92 ± 0.023	6.291LeuAsp: 6.291 ± 0.07	5.125LeuGlu: 5.125 ± 0.063	3.095LeuPhe: 3.095 ± 0.046	9.743LeuGly: 9.743 ± 0.088	2.397LeuHis: 2.397 ± 0.043	3.14LeuIle: 3.14 ± 0.048	2.684LeuLys: 2.684 ± 0.044	12.168LeuLeu: 12.168 ± 0.13	2.141LeuMet: 2.141 ± 0.035	2.136LeuAsn: 2.136 ± 0.035	7.512LeuPro: 7.512 ± 0.077	4.456LeuGln: 4.456 ± 0.052	9.984LeuArg: 9.984 ± 0.08	5.402LeuSer: 5.402 ± 0.057	4.52LeuThr: 4.52 ± 0.046	8.545LeuVal: 8.545 ± 0.069	1.356LeuTrp: 1.356 ± 0.027	1.872LeuTyr: 1.872 ± 0.032	0.0LeuXaa: 0.0 ± 0.0
Met	2.65MetAla: 2.65 ± 0.036	0.134MetCys: 0.134 ± 0.008	1.002MetAsp: 1.002 ± 0.025	0.774MetGlu: 0.774 ± 0.02	0.553MetPhe: 0.553 ± 0.019	1.482MetGly: 1.482 ± 0.03	0.459MetHis: 0.459 ± 0.015	0.721MetIle: 0.721 ± 0.021	0.659MetLys: 0.659 ± 0.021	2.251MetLeu: 2.251 ± 0.034	0.439MetMet: 0.439 ± 0.018	0.635MetAsn: 0.635 ± 0.02	1.404MetPro: 1.404 ± 0.024	0.822MetGln: 0.822 ± 0.019	1.617MetArg: 1.617 ± 0.027	1.32MetSer: 1.32 ± 0.028	1.261MetThr: 1.261 ± 0.027	1.404MetVal: 1.404 ± 0.03	0.174MetTrp: 0.174 ± 0.009	0.304MetTyr: 0.304 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.494AsnAla: 2.494 ± 0.037	0.199AsnCys: 0.199 ± 0.011	0.973AsnAsp: 0.973 ± 0.026	0.884AsnGlu: 0.884 ± 0.024	0.665AsnPhe: 0.665 ± 0.02	1.685AsnGly: 1.685 ± 0.032	0.403AsnHis: 0.403 ± 0.016	0.67AsnIle: 0.67 ± 0.023	0.457AsnLys: 0.457 ± 0.019	2.156AsnLeu: 2.156 ± 0.039	0.353AsnMet: 0.353 ± 0.015	0.465AsnAsn: 0.465 ± 0.02	1.476AsnPro: 1.476 ± 0.034	0.606AsnGln: 0.606 ± 0.02	1.426AsnArg: 1.426 ± 0.027	0.697AsnSer: 0.697 ± 0.023	0.911AsnThr: 0.911 ± 0.022	1.474AsnVal: 1.474 ± 0.03	0.288AsnTrp: 0.288 ± 0.013	0.446AsnTyr: 0.446 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	11.61ProAla: 11.61 ± 0.13	0.435ProCys: 0.435 ± 0.019	3.655ProAsp: 3.655 ± 0.048	3.161ProGlu: 3.161 ± 0.042	1.721ProPhe: 1.721 ± 0.03	6.524ProGly: 6.524 ± 0.075	1.105ProHis: 1.105 ± 0.027	1.649ProIle: 1.649 ± 0.033	1.134ProLys: 1.134 ± 0.026	6.139ProLeu: 6.139 ± 0.055	1.3ProMet: 1.3 ± 0.025	1.059ProAsn: 1.059 ± 0.024	4.868ProPro: 4.868 ± 0.087	2.226ProGln: 2.226 ± 0.034	4.51ProArg: 4.51 ± 0.059	2.836ProSer: 2.836 ± 0.05	2.832ProThr: 2.832 ± 0.046	4.884ProVal: 4.884 ± 0.055	0.983ProTrp: 0.983 ± 0.024	1.048ProTyr: 1.048 ± 0.023	0.0ProXaa: 0.0 ± 0.0
Gln	6.452GlnAla: 6.452 ± 0.084	0.249GlnCys: 0.249 ± 0.012	1.429GlnAsp: 1.429 ± 0.028	1.25GlnGlu: 1.25 ± 0.032	0.996GlnPhe: 0.996 ± 0.024	2.754GlnGly: 2.754 ± 0.04	0.805GlnHis: 0.805 ± 0.022	1.2GlnIle: 1.2 ± 0.024	0.672GlnLys: 0.672 ± 0.02	3.732GlnLeu: 3.732 ± 0.048	0.691GlnMet: 0.691 ± 0.022	0.525GlnAsn: 0.525 ± 0.017	2.38GlnPro: 2.38 ± 0.038	1.626GlnGln: 1.626 ± 0.038	3.941GlnArg: 3.941 ± 0.06	1.379GlnSer: 1.379 ± 0.026	1.495GlnThr: 1.495 ± 0.029	2.959GlnVal: 2.959 ± 0.04	0.58GlnTrp: 0.58 ± 0.019	0.549GlnTyr: 0.549 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	11.106ArgAla: 11.106 ± 0.093	0.812ArgCys: 0.812 ± 0.025	4.201ArgAsp: 4.201 ± 0.054	4.846ArgGlu: 4.846 ± 0.066	2.874ArgPhe: 2.874 ± 0.043	6.237ArgGly: 6.237 ± 0.069	2.492ArgHis: 2.492 ± 0.049	3.387ArgIle: 3.387 ± 0.04	1.445ArgLys: 1.445 ± 0.03	10.893ArgLeu: 10.893 ± 0.089	1.929ArgMet: 1.929 ± 0.035	1.427ArgAsn: 1.427 ± 0.027	5.411ArgPro: 5.411 ± 0.069	3.298ArgGln: 3.298 ± 0.052	8.71ArgArg: 8.71 ± 0.106	3.995ArgSer: 3.995 ± 0.047	3.424ArgThr: 3.424 ± 0.048	6.108ArgVal: 6.108 ± 0.059	1.645ArgTrp: 1.645 ± 0.034	1.788ArgTyr: 1.788 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	6.35SerAla: 6.35 ± 0.061	0.388SerCys: 0.388 ± 0.016	2.129SerAsp: 2.129 ± 0.036	2.031SerGlu: 2.031 ± 0.038	1.734SerPhe: 1.734 ± 0.038	4.657SerGly: 4.657 ± 0.053	0.994SerHis: 0.994 ± 0.022	1.738SerIle: 1.738 ± 0.037	0.916SerLys: 0.916 ± 0.028	5.005SerLeu: 5.005 ± 0.058	0.982SerMet: 0.982 ± 0.024	0.958SerAsn: 0.958 ± 0.024	2.95SerPro: 2.95 ± 0.042	1.395SerGln: 1.395 ± 0.029	3.419SerArg: 3.419 ± 0.048	2.307SerSer: 2.307 ± 0.04	2.437SerThr: 2.437 ± 0.039	3.445SerVal: 3.445 ± 0.044	0.613SerTrp: 0.613 ± 0.017	0.936SerTyr: 0.936 ± 0.027	0.0SerXaa: 0.0 ± 0.0
Thr	6.104ThrAla: 6.104 ± 0.059	0.35ThrCys: 0.35 ± 0.014	2.169ThrAsp: 2.169 ± 0.036	2.077ThrGlu: 2.077 ± 0.039	1.202ThrPhe: 1.202 ± 0.023	4.367ThrGly: 4.367 ± 0.054	0.96ThrHis: 0.96 ± 0.024	1.532ThrIle: 1.532 ± 0.03	0.811ThrLys: 0.811 ± 0.028	6.139ThrLeu: 6.139 ± 0.061	0.9ThrMet: 0.9 ± 0.022	0.886ThrAsn: 0.886 ± 0.026	3.697ThrPro: 3.697 ± 0.048	1.453ThrGln: 1.453 ± 0.027	3.613ThrArg: 3.613 ± 0.053	1.991ThrSer: 1.991 ± 0.037	2.41ThrThr: 2.41 ± 0.051	3.907ThrVal: 3.907 ± 0.048	0.646ThrTrp: 0.646 ± 0.021	0.838ThrTyr: 0.838 ± 0.025	0.0ThrXaa: 0.0 ± 0.0
Val	10.728ValAla: 10.728 ± 0.088	0.73ValCys: 0.73 ± 0.023	4.513ValAsp: 4.513 ± 0.057	4.233ValGlu: 4.233 ± 0.052	2.58ValPhe: 2.58 ± 0.045	5.914ValGly: 5.914 ± 0.069	1.649ValHis: 1.649 ± 0.033	2.753ValIle: 2.753 ± 0.045	1.736ValLys: 1.736 ± 0.04	8.747ValLeu: 8.747 ± 0.072	1.554ValMet: 1.554 ± 0.032	1.678ValAsn: 1.678 ± 0.031	4.596ValPro: 4.596 ± 0.056	2.713ValGln: 2.713 ± 0.037	5.911ValArg: 5.911 ± 0.058	3.58ValSer: 3.58 ± 0.047	3.734ValThr: 3.734 ± 0.052	6.511ValVal: 6.511 ± 0.074	1.003ValTrp: 1.003 ± 0.024	1.448ValTyr: 1.448 ± 0.027	0.0ValXaa: 0.0 ± 0.0
Trp	1.713TrpAla: 1.713 ± 0.038	0.194TrpCys: 0.194 ± 0.009	0.652TrpAsp: 0.652 ± 0.021	0.545TrpGlu: 0.545 ± 0.017	0.534TrpPhe: 0.534 ± 0.017	1.016TrpGly: 1.016 ± 0.027	0.36TrpHis: 0.36 ± 0.015	0.601TrpIle: 0.601 ± 0.018	0.321TrpLys: 0.321 ± 0.014	2.159TrpLeu: 2.159 ± 0.042	0.367TrpMet: 0.367 ± 0.013	0.342TrpAsn: 0.342 ± 0.015	0.935TrpPro: 0.935 ± 0.029	0.798TrpGln: 0.798 ± 0.025	1.65TrpArg: 1.65 ± 0.032	0.775TrpSer: 0.775 ± 0.021	0.816TrpThr: 0.816 ± 0.023	1.097TrpVal: 1.097 ± 0.025	0.305TrpTrp: 0.305 ± 0.013	0.265TrpTyr: 0.265 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.518TyrAla: 2.518 ± 0.04	0.186TyrCys: 0.186 ± 0.01	1.091TyrAsp: 1.091 ± 0.029	0.986TyrGlu: 0.986 ± 0.024	0.699TyrPhe: 0.699 ± 0.02	1.796TyrGly: 1.796 ± 0.032	0.394TyrHis: 0.394 ± 0.017	0.495TyrIle: 0.495 ± 0.017	0.42TyrLys: 0.42 ± 0.018	2.109TyrLeu: 2.109 ± 0.037	0.277TyrMet: 0.277 ± 0.014	0.418TyrAsn: 0.418 ± 0.014	0.958TyrPro: 0.958 ± 0.023	0.623TyrGln: 0.623 ± 0.018	1.741TyrArg: 1.741 ± 0.034	0.804TyrSer: 0.804 ± 0.021	0.957TyrThr: 0.957 ± 0.027	1.44TyrVal: 1.44 ± 0.028	0.294TyrTrp: 0.294 ± 0.011	0.447TyrTyr: 0.447 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5527 proteins (1811574 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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