Oesophagostomum dentatum (Nodular worm)
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 25133 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B1SKA4|A0A0B1SKA4_OESDE Nanos-type domain-containing protein OS=Oesophagostomum dentatum OX=61180 GN=OESDEN_15968 PE=3 SV=1
MM1 pKa = 7.49 LAKK4 pKa = 10.58 LLIGLLGVTSCLSDD18 pKa = 3.13 TSFTDD23 pKa = 3.07 TTTDD27 pKa = 2.56 IFTASVTTATTSSAAPTPAPTLDD50 pKa = 3.2 QCSCTDD56 pKa = 3.29 KK57 pKa = 11.32 NIWIDD62 pKa = 3.61 VYY64 pKa = 10.89 FLIDD68 pKa = 3.32 SSKK71 pKa = 11.57 AMTSPGFDD79 pKa = 2.89 GATAFVEE86 pKa = 4.31 SAVYY90 pKa = 10.85 KK91 pKa = 7.79 MTIGQAVGQQTRR103 pKa = 11.84 VGFITYY109 pKa = 10.26 SATASKK115 pKa = 10.9 NYY117 pKa = 10.4 DD118 pKa = 2.97 LTAFNSADD126 pKa = 3.69 DD127 pKa = 5.12 VIDD130 pKa = 4.61 ADD132 pKa = 4.96 LAFDD136 pKa = 4.52 GNSGTNIEE144 pKa = 4.36 SS145 pKa = 3.32
Molecular weight: 15.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.732
IPC_protein 3.719
Toseland 3.478
ProMoST 3.935
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.745
Rodwell 3.541
Grimsley 3.401
Solomon 3.732
Lehninger 3.681
Nozaki 3.872
DTASelect 4.19
Thurlkill 3.567
EMBOSS 3.745
Sillero 3.846
Patrickios 1.901
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.792
Protein with the highest isoelectric point:
>tr|A0A0B1S8R4|A0A0B1S8R4_OESDE G_PROTEIN_RECEP_F1_2 domain-containing protein (Fragment) OS=Oesophagostomum dentatum OX=61180 GN=OESDEN_20030 PE=4 SV=1
MM1 pKa = 6.88 VRR3 pKa = 11.84 RR4 pKa = 11.84 RR5 pKa = 11.84 TASPRR10 pKa = 11.84 PSAPVHH16 pKa = 5.47 SAPRR20 pKa = 11.84 PAPAMRR26 pKa = 11.84 SAPPPAPAPTPMPARR41 pKa = 11.84 TAPPMAGAPPPAAPAQPGLMAQMAATAGGVAIGSAVV77 pKa = 2.93
Molecular weight: 7.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.462
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.281
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.047
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.153
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
25133
0
25133
5464594
26
4733
217.4
24.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.244 ± 0.016
2.222 ± 0.015
5.343 ± 0.015
6.595 ± 0.023
4.289 ± 0.014
5.833 ± 0.025
2.353 ± 0.007
5.447 ± 0.016
5.836 ± 0.018
9.082 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.673 ± 0.009
4.096 ± 0.011
5.103 ± 0.02
3.786 ± 0.012
5.986 ± 0.017
7.514 ± 0.018
5.557 ± 0.016
6.647 ± 0.014
1.178 ± 0.007
3.216 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here