Ventosimonas gracilis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Ventosimonadaceae; Ventosimonas

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2412 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A139SVK8|A0A139SVK8_9GAMM Uncharacterized protein OS=Ventosimonas gracilis OX=1680762 GN=AXE65_13035 PE=4 SV=1
MM1 pKa = 7.14TFVVTDD7 pKa = 3.25NCIKK11 pKa = 10.54CKK13 pKa = 10.0YY14 pKa = 7.47TDD16 pKa = 3.85CVEE19 pKa = 4.15VCPVDD24 pKa = 4.13CFYY27 pKa = 11.12EE28 pKa = 4.9GPNFLVIHH36 pKa = 6.99PDD38 pKa = 3.12EE39 pKa = 5.91CIDD42 pKa = 4.16CALCEE47 pKa = 4.36PEE49 pKa = 5.63CPAQAIFSEE58 pKa = 4.92DD59 pKa = 3.69EE60 pKa = 4.29VPDD63 pKa = 4.01DD64 pKa = 3.48MQEE67 pKa = 4.23FIEE70 pKa = 4.83LNAEE74 pKa = 4.66LAQSWSNITEE84 pKa = 4.31KK85 pKa = 10.84KK86 pKa = 10.31DD87 pKa = 3.57ALPDD91 pKa = 3.79AEE93 pKa = 5.09DD94 pKa = 3.36WDD96 pKa = 4.16GVEE99 pKa = 5.02NKK101 pKa = 9.68IKK103 pKa = 10.51YY104 pKa = 9.97LEE106 pKa = 4.05RR107 pKa = 5.72

Molecular weight: 12.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A139SV68|A0A139SV68_9GAMM Uncharacterized protein OS=Ventosimonas gracilis OX=1680762 GN=AXE65_01010 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.73GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2412

0

2412

755995

31

2260

313.4

34.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.213 ± 0.05

1.164 ± 0.02

4.968 ± 0.036

5.779 ± 0.05

3.745 ± 0.033

7.63 ± 0.047

2.232 ± 0.024

5.156 ± 0.039

4.022 ± 0.034

12.332 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.196 ± 0.025

3.143 ± 0.033

4.704 ± 0.035

5.053 ± 0.048

6.348 ± 0.044

5.86 ± 0.036

4.401 ± 0.029

6.141 ± 0.039

1.402 ± 0.022

2.509 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski