Faeces associated gemycircularvirus 10
Average proteome isoelectric point is 7.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|T1YRV6|T1YRV6_9VIRU Capsid protein OS=Faeces associated gemycircularvirus 10 OX=1391025 PE=4 SV=1
MM1 pKa = 7.5 SNSTLLSVTLCPHH14 pKa = 6.03 YY15 pKa = 10.8 LCPCDD20 pKa = 5.08 GLDD23 pKa = 3.19 YY24 pKa = 10.83 WRR26 pKa = 11.84 VLDD29 pKa = 4.57 HH30 pKa = 6.78 LSEE33 pKa = 4.43 VPAEE37 pKa = 4.16 CIIGRR42 pKa = 11.84 EE43 pKa = 3.97 AHH45 pKa = 6.81 ADD47 pKa = 3.53 GGTHH51 pKa = 6.7 LHH53 pKa = 6.66 AFCDD57 pKa = 4.23 FGEE60 pKa = 4.35 RR61 pKa = 11.84 FYY63 pKa = 10.87 TADD66 pKa = 3.36 PRR68 pKa = 11.84 RR69 pKa = 11.84 FDD71 pKa = 3.24 VDD73 pKa = 4.03 GYY75 pKa = 10.65 HH76 pKa = 7.43 PNVQPFGRR84 pKa = 11.84 TPYY87 pKa = 10.29 KK88 pKa = 10.43 GWDD91 pKa = 3.43 YY92 pKa = 11.11 AIKK95 pKa = 10.84 DD96 pKa = 3.55 GDD98 pKa = 4.3 VVCGGLEE105 pKa = 3.97 RR106 pKa = 11.84 PGTGPGTDD114 pKa = 3.02 VSGAGSQWDD123 pKa = 4.01 RR124 pKa = 11.84 IVGAEE129 pKa = 4.23 TPDD132 pKa = 3.51 EE133 pKa = 4.15 FWRR136 pKa = 11.84 LIRR139 pKa = 11.84 EE140 pKa = 4.38 LAPRR144 pKa = 11.84 TLLTNFNSVRR154 pKa = 11.84 AYY156 pKa = 10.65 ADD158 pKa = 2.69 WHH160 pKa = 5.55 YY161 pKa = 10.75 RR162 pKa = 11.84 PVVAPYY168 pKa = 9.03 EE169 pKa = 4.33 SPPEE173 pKa = 3.9 IEE175 pKa = 4.44 FDD177 pKa = 3.48 LSGVEE182 pKa = 4.74 EE183 pKa = 4.05 LRR185 pKa = 11.84 EE186 pKa = 4.09 WVCNNLSRR194 pKa = 11.84 TRR196 pKa = 11.84 RR197 pKa = 11.84 PKK199 pKa = 10.61 SLVLYY204 pKa = 10.61 GEE206 pKa = 4.24 SRR208 pKa = 11.84 LGKK211 pKa = 7.36 TLWARR216 pKa = 11.84 SLGRR220 pKa = 11.84 HH221 pKa = 5.63 VYY223 pKa = 10.33 NCLQFNVDD231 pKa = 3.92 DD232 pKa = 3.77 MRR234 pKa = 11.84 GDD236 pKa = 3.52 VEE238 pKa = 4.03 EE239 pKa = 4.36 ALYY242 pKa = 10.91 AVFDD246 pKa = 4.22 DD247 pKa = 3.87 MQGGFKK253 pKa = 10.6 YY254 pKa = 10.49 FPSYY258 pKa = 10.47 KK259 pKa = 9.96 GWLGAQSKK267 pKa = 7.6 FTVTDD272 pKa = 3.76 KK273 pKa = 11.37 YY274 pKa = 10.65 RR275 pKa = 11.84 GKK277 pKa = 9.31 TSVIWGRR284 pKa = 11.84 PTIWLCNEE292 pKa = 3.98 SPRR295 pKa = 11.84 DD296 pKa = 3.75 FTDD299 pKa = 4.49 VDD301 pKa = 4.2 MTWLEE306 pKa = 4.1 ANCVFVEE313 pKa = 4.07 VTNPIFRR320 pKa = 11.84 ANIEE324 pKa = 3.95
Molecular weight: 36.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.968
IPC2_protein 5.08
IPC_protein 5.041
Toseland 4.991
ProMoST 5.13
Dawson 5.054
Bjellqvist 5.194
Wikipedia 4.94
Rodwell 4.965
Grimsley 4.914
Solomon 5.054
Lehninger 5.016
Nozaki 5.181
DTASelect 5.359
Thurlkill 5.016
EMBOSS 5.003
Sillero 5.245
Patrickios 3.63
IPC_peptide 5.054
IPC2_peptide 5.245
IPC2.peptide.svr19 5.168
Protein with the highest isoelectric point:
>tr|T1YRV6|T1YRV6_9VIRU Capsid protein OS=Faeces associated gemycircularvirus 10 OX=1391025 PE=4 SV=1
MM1 pKa = 7.54 PGYY4 pKa = 9.94 RR5 pKa = 11.84 SKK7 pKa = 11.0 RR8 pKa = 11.84 KK9 pKa = 9.26 YY10 pKa = 10.18 RR11 pKa = 11.84 RR12 pKa = 11.84 TSRR15 pKa = 11.84 KK16 pKa = 8.74 GGRR19 pKa = 11.84 SLRR22 pKa = 11.84 RR23 pKa = 11.84 RR24 pKa = 11.84 SGYY27 pKa = 9.84 RR28 pKa = 11.84 KK29 pKa = 8.82 SSKK32 pKa = 9.72 GRR34 pKa = 11.84 RR35 pKa = 11.84 FLRR38 pKa = 11.84 GRR40 pKa = 11.84 RR41 pKa = 11.84 GMSRR45 pKa = 11.84 KK46 pKa = 9.7 ALLNVTSRR54 pKa = 11.84 KK55 pKa = 9.78 KK56 pKa = 10.19 RR57 pKa = 11.84 DD58 pKa = 3.21 TMLSYY63 pKa = 11.18 TNVSTSSQIGSANYY77 pKa = 9.47 VVGPAVLAGGAQRR90 pKa = 11.84 FFLWCATARR99 pKa = 11.84 GISTYY104 pKa = 11.36 NNFDD108 pKa = 3.41 ASLIDD113 pKa = 3.6 QSSRR117 pKa = 11.84 TATNCYY123 pKa = 9.02 MRR125 pKa = 11.84 GLKK128 pKa = 9.77 EE129 pKa = 4.37 RR130 pKa = 11.84 IRR132 pKa = 11.84 ISTDD136 pKa = 3.54 DD137 pKa = 3.51 GMPWLWRR144 pKa = 11.84 RR145 pKa = 11.84 ICFTSRR151 pKa = 11.84 NLNLRR156 pKa = 11.84 GYY158 pKa = 7.87 QTGAGTSYY166 pKa = 11.29 SPAFLTSAGFMRR178 pKa = 11.84 GVNQPYY184 pKa = 9.6 NDD186 pKa = 3.74 SYY188 pKa = 11.8 INDD191 pKa = 4.57 LLWKK195 pKa = 8.89 GTLGKK200 pKa = 10.11 DD201 pKa = 2.85 WNDD204 pKa = 3.0 FMTATVDD211 pKa = 3.29 NRR213 pKa = 11.84 RR214 pKa = 11.84 VDD216 pKa = 3.51 VKK218 pKa = 10.96 YY219 pKa = 11.27 DD220 pKa = 3.2 KK221 pKa = 8.68 TTTIAAGNEE230 pKa = 4.02 EE231 pKa = 4.21 GCIRR235 pKa = 11.84 EE236 pKa = 4.09 FNRR239 pKa = 11.84 WHH241 pKa = 6.97 PMNKK245 pKa = 9.63 SLVYY249 pKa = 10.49 DD250 pKa = 3.94 DD251 pKa = 5.57 EE252 pKa = 4.5 EE253 pKa = 6.46 AGDD256 pKa = 3.79 EE257 pKa = 4.15 AAGSVFSVTDD267 pKa = 3.22 KK268 pKa = 11.26 RR269 pKa = 11.84 GMGDD273 pKa = 3.94 YY274 pKa = 11.04 YY275 pKa = 11.65 VMDD278 pKa = 3.57 IFDD281 pKa = 4.56 PRR283 pKa = 11.84 SGSTSASHH291 pKa = 7.44 LSFRR295 pKa = 11.84 PDD297 pKa = 2.7 ATLYY301 pKa = 7.81 WHH303 pKa = 6.64 EE304 pKa = 4.21 RR305 pKa = 3.47
Molecular weight: 34.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.31
IPC2_protein 9.663
IPC_protein 10.335
Toseland 10.218
ProMoST 10.072
Dawson 10.438
Bjellqvist 10.175
Wikipedia 10.672
Rodwell 10.643
Grimsley 10.54
Solomon 10.496
Lehninger 10.438
Nozaki 10.233
DTASelect 10.175
Thurlkill 10.292
EMBOSS 10.643
Sillero 10.365
Patrickios 10.116
IPC_peptide 10.482
IPC2_peptide 9.151
IPC2.peptide.svr19 8.614
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
629
305
324
314.5
35.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.041 ± 0.358
2.226 ± 0.635
7.154 ± 0.414
4.61 ± 1.38
4.452 ± 0.359
8.903 ± 0.193
1.749 ± 0.531
3.18 ± 0.069
3.816 ± 0.765
7.154 ± 0.642
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.226 ± 0.731
4.452 ± 0.551
4.452 ± 1.269
1.59 ± 0.034
9.857 ± 1.579
7.631 ± 1.758
6.677 ± 0.6
6.041 ± 1.235
2.862 ± 0.393
4.928 ± 0.22
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here