Fibrella sp. ES10-3-2-2

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Fibrella; unclassified Fibrella

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5038 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6E758|A0A1W6E758_9BACT Peptidase C69 OS=Fibrella sp. ES10-3-2-2 OX=1834519 GN=A6C57_12990 PE=3 SV=1
MM1 pKa = 7.67LYY3 pKa = 10.16YY4 pKa = 10.72VKK6 pKa = 9.94TYY8 pKa = 9.96FLVGMRR14 pKa = 11.84PIVLVWALLFGLLSTAQTNAQTLPCNYY41 pKa = 7.8RR42 pKa = 11.84TLTTNNYY49 pKa = 9.63SGAFIVNSRR58 pKa = 11.84TGGTGGFLGIGAGNVTNEE76 pKa = 3.85GRR78 pKa = 11.84VIDD81 pKa = 4.39ADD83 pKa = 4.11LNNFASINTTLTLASGGEE101 pKa = 3.92ISVDD105 pKa = 3.02AGGTVVFGAGATAGYY120 pKa = 10.16VVDD123 pKa = 4.92GSSGLGANLLGSVTIITYY141 pKa = 10.81LNGIEE146 pKa = 4.03QEE148 pKa = 4.13RR149 pKa = 11.84SNSSSLLDD157 pKa = 3.59LVLLSGSGRR166 pKa = 11.84RR167 pKa = 11.84TLGFVTTKK175 pKa = 10.75NFDD178 pKa = 3.35EE179 pKa = 4.33VQIRR183 pKa = 11.84VSSLISLLTSLPVYY197 pKa = 10.68YY198 pKa = 10.13PFVQYY203 pKa = 8.75KK204 pKa = 8.76TLGATATATSASGLTTADD222 pKa = 3.07GSVALAVTGGRR233 pKa = 11.84APFTYY238 pKa = 9.89LWSNGATTANLTNVLPGTYY257 pKa = 9.91SVTVTDD263 pKa = 5.34ANGCTTTASATVGIKK278 pKa = 9.85VAACPVPGQNGFTKK292 pKa = 10.57FSFATAPTITGQGVGRR308 pKa = 11.84KK309 pKa = 8.77GRR311 pKa = 11.84YY312 pKa = 8.81VNVASIGGVAVDD324 pKa = 4.48VIGEE328 pKa = 4.26VITYY332 pKa = 10.37SGTADD337 pKa = 3.02ATFPRR342 pKa = 11.84FDD344 pKa = 4.04NFSSTSGAFLARR356 pKa = 11.84YY357 pKa = 8.62AISGASTAPAGLTSTVRR374 pKa = 11.84WSVVKK379 pKa = 9.9TGTNLPVPFQGAFTVGDD396 pKa = 3.43IDD398 pKa = 5.37NDD400 pKa = 3.48VTLNSTTLEE409 pKa = 4.1SIIVNKK415 pKa = 10.31SDD417 pKa = 3.31LYY419 pKa = 10.82SYY421 pKa = 10.88KK422 pKa = 10.52LSSPTNTSVISSSASPTIKK441 pKa = 10.58FQGTLNQVGIDD452 pKa = 3.79GVDD455 pKa = 3.31PAFTVALAYY464 pKa = 10.62VGVSSFEE471 pKa = 3.6VTYY474 pKa = 10.94AKK476 pKa = 10.49VGSSSGTANFPFDD489 pKa = 3.81GEE491 pKa = 4.3GGIVFLASTCTPVLDD506 pKa = 3.87TDD508 pKa = 4.06GDD510 pKa = 4.31GVANAIDD517 pKa = 3.91TDD519 pKa = 4.09DD520 pKa = 5.29DD521 pKa = 4.32NDD523 pKa = 5.04GILDD527 pKa = 3.8DD528 pKa = 4.16TEE530 pKa = 5.75GGVFVDD536 pKa = 4.19SDD538 pKa = 3.85GDD540 pKa = 4.27GISNALDD547 pKa = 3.95LDD549 pKa = 3.94SDD551 pKa = 4.27GDD553 pKa = 4.26GIPDD557 pKa = 4.04NIEE560 pKa = 3.83AQTTAGYY567 pKa = 9.26IAPGTAVDD575 pKa = 3.65AAGLLTSYY583 pKa = 9.25TATNGLIPVNTDD595 pKa = 2.57GTDD598 pKa = 3.09TADD601 pKa = 4.29YY602 pKa = 11.23LDD604 pKa = 5.3LDD606 pKa = 4.08SDD608 pKa = 3.79NDD610 pKa = 3.66TRR612 pKa = 11.84TDD614 pKa = 3.35TVEE617 pKa = 4.69AGITLANADD626 pKa = 4.39DD627 pKa = 5.38DD628 pKa = 5.92KK629 pKa = 11.84DD630 pKa = 4.08GLDD633 pKa = 4.16NSPDD637 pKa = 3.59TNDD640 pKa = 3.51NLFGPVNAGITNPLTFYY657 pKa = 10.15PNNGTEE663 pKa = 4.14VLWRR667 pKa = 11.84VKK669 pKa = 10.37RR670 pKa = 11.84GAFTYY675 pKa = 9.93GNCALATFSGQFVVGIPSSGVLTIPITTSIDD706 pKa = 3.19GAITITSVSGSGISSVPASVTAILIAGQTTLSIPIAYY743 pKa = 9.48DD744 pKa = 3.24GSGVIGTRR752 pKa = 11.84NLTVSSVDD760 pKa = 3.23ATGTCAPIVAVIGLADD776 pKa = 4.06LTTSIGIPVPTLIGAQTSSLPISVSNIGSAATTGPITTTLTLPASVTAPATFTSNGFGCTTTGTSVSCTSSAILANGSSTTFAVPITPPLATVGTTLSFTNTVMTTQEE884 pKa = 3.38ISVTNNTGTSTALVTGAPDD903 pKa = 3.56LAVSIGQPSPALVVAQTSTIPVSVSNVGTIPTIGPITVTLTIPASVTAPATFTNNGFGCSTSGGTITCTSAGPIANAASLTFGIPVTPLSAALGTTPTFSGLVATTGDD1011 pKa = 3.8IITANNSATMTANTAVACAVGSAIPILKK1039 pKa = 10.25

Molecular weight: 104.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6E9W9|A0A1W6E9W9_9BACT DUF4918 domain-containing protein OS=Fibrella sp. ES10-3-2-2 OX=1834519 GN=A6C57_18830 PE=4 SV=1
MM1 pKa = 7.98PKK3 pKa = 10.22QKK5 pKa = 9.71TNSAAKK11 pKa = 10.06KK12 pKa = 9.44RR13 pKa = 11.84FKK15 pKa = 10.53LTGTGKK21 pKa = 10.6LKK23 pKa = 10.41RR24 pKa = 11.84KK25 pKa = 8.99HH26 pKa = 6.27AFHH29 pKa = 6.83SHH31 pKa = 5.99ILTKK35 pKa = 9.89KK36 pKa = 4.76TTKK39 pKa = 9.74QKK41 pKa = 10.98RR42 pKa = 11.84NLAHH46 pKa = 6.91SSTVHH51 pKa = 5.26PTNMTRR57 pKa = 11.84IKK59 pKa = 10.88ALLRR63 pKa = 11.84LSS65 pKa = 3.59

Molecular weight: 7.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5038

0

5038

1868441

26

5706

370.9

41.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.967 ± 0.04

0.742 ± 0.013

5.343 ± 0.027

4.816 ± 0.03

4.282 ± 0.023

7.482 ± 0.031

1.744 ± 0.017

5.444 ± 0.027

4.56 ± 0.034

10.111 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.132 ± 0.016

4.523 ± 0.033

4.744 ± 0.023

4.395 ± 0.025

5.415 ± 0.032

6.123 ± 0.034

7.006 ± 0.054

7.105 ± 0.027

1.314 ± 0.015

3.753 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski