Sphingobium sp. GW456-12-10-14-TSB1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingobium; unclassified Sphingobium

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4472 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A243PFT8|A0A243PFT8_9SPHN 2-isopropylmalate synthase OS=Sphingobium sp. GW456-12-10-14-TSB1 OX=1987165 GN=leuA PE=3 SV=1
MM1 pKa = 7.79PKK3 pKa = 10.4LIVVNRR9 pKa = 11.84AGEE12 pKa = 4.04EE13 pKa = 3.9QAVDD17 pKa = 3.52GDD19 pKa = 3.97NGLSVMEE26 pKa = 4.52VIRR29 pKa = 11.84DD30 pKa = 3.58NGFDD34 pKa = 3.96EE35 pKa = 5.76LLALCGGCCSCATCHH50 pKa = 6.48VYY52 pKa = 10.53VDD54 pKa = 3.75PAFADD59 pKa = 3.61SLPAMSEE66 pKa = 4.17DD67 pKa = 4.28EE68 pKa = 4.68NDD70 pKa = 4.1LLDD73 pKa = 5.03SSDD76 pKa = 3.51HH77 pKa = 6.92RR78 pKa = 11.84NDD80 pKa = 3.21TSRR83 pKa = 11.84LSCQVVLTDD92 pKa = 4.3ALDD95 pKa = 3.82GLRR98 pKa = 11.84VTIAPEE104 pKa = 3.78DD105 pKa = 3.45

Molecular weight: 11.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A243PFM7|A0A243PFM7_9SPHN Energy transducer TonB OS=Sphingobium sp. GW456-12-10-14-TSB1 OX=1987165 GN=CA262_11730 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.33RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 5.19GYY18 pKa = 7.59RR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84NIIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4472

0

4472

1434412

34

12366

320.8

34.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.157 ± 0.058

0.829 ± 0.014

6.181 ± 0.028

5.024 ± 0.035

3.484 ± 0.024

8.758 ± 0.036

2.074 ± 0.024

5.257 ± 0.025

2.987 ± 0.031

9.999 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.604 ± 0.023

2.527 ± 0.029

5.318 ± 0.024

3.293 ± 0.023

7.345 ± 0.054

5.306 ± 0.031

5.216 ± 0.046

6.98 ± 0.035

1.434 ± 0.015

2.229 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski