Bathycoccus sp. RCC1105 virus BpV1
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 203 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E5ESN4|E5ESN4_9PHYC Uncharacterized protein OS=Bathycoccus sp. RCC1105 virus BpV1 OX=880159 GN=BpV1_016 PE=4 SV=1
MM1 pKa = 6.92 EE2 pKa = 4.19 TQIIDD7 pKa = 3.23 VDD9 pKa = 3.96 FDD11 pKa = 4.77 DD12 pKa = 4.95 GFKK15 pKa = 10.88 SIAKK19 pKa = 9.69 IIKK22 pKa = 9.25 DD23 pKa = 3.76 CYY25 pKa = 10.55 SEE27 pKa = 4.85 YY28 pKa = 10.82 EE29 pKa = 4.27 VALLEE34 pKa = 4.22 YY35 pKa = 10.49 YY36 pKa = 11.04 GDD38 pKa = 4.23 GEE40 pKa = 4.25 WDD42 pKa = 3.54 FDD44 pKa = 4.17 TEE46 pKa = 4.22 EE47 pKa = 4.22 TTTIMKK53 pKa = 10.49 DD54 pKa = 3.56 SVSGFYY60 pKa = 9.83 DD61 pKa = 3.42 TTDD64 pKa = 3.4 LEE66 pKa = 4.5 NTGLYY71 pKa = 9.47 EE72 pKa = 3.89 KK73 pKa = 9.93 LKK75 pKa = 9.56 NGMYY79 pKa = 10.39 VEE81 pKa = 4.21 VDD83 pKa = 3.22 EE84 pKa = 6.25 SDD86 pKa = 3.63 FEE88 pKa = 4.56 YY89 pKa = 11.08 EE90 pKa = 4.2 LASSEE95 pKa = 4.17 DD96 pKa = 4.15 DD97 pKa = 3.58 EE98 pKa = 5.61 SEE100 pKa = 4.44 SDD102 pKa = 3.56 VSLDD106 pKa = 4.23 DD107 pKa = 4.45 EE108 pKa = 4.64 EE109 pKa = 4.43 FF110 pKa = 3.93
Molecular weight: 12.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.921
IPC2_protein 3.427
IPC_protein 3.414
Toseland 3.198
ProMoST 3.579
Dawson 3.401
Bjellqvist 3.567
Wikipedia 3.338
Rodwell 3.236
Grimsley 3.109
Solomon 3.376
Lehninger 3.338
Nozaki 3.528
DTASelect 3.719
Thurlkill 3.261
EMBOSS 3.35
Sillero 3.528
Patrickios 0.54
IPC_peptide 3.376
IPC2_peptide 3.503
IPC2.peptide.svr19 3.625
Protein with the highest isoelectric point:
>tr|E5ESF7|E5ESF7_9PHYC Uncharacterized protein OS=Bathycoccus sp. RCC1105 virus BpV1 OX=880159 GN=BpV1_142c PE=4 SV=1
MM1 pKa = 7.43 ILVMSKK7 pKa = 10.14 PLPLSGSEE15 pKa = 3.78 PKK17 pKa = 9.05 YY18 pKa = 8.32 TQRR21 pKa = 11.84 LWGRR25 pKa = 11.84 TVGVGNNNCYY35 pKa = 10.51 AYY37 pKa = 10.43 AVGDD41 pKa = 3.92 YY42 pKa = 11.2 EE43 pKa = 5.75 KK44 pKa = 10.42 MRR46 pKa = 11.84 LQKK49 pKa = 10.55 SIPGEE54 pKa = 3.63 RR55 pKa = 11.84 AGIRR59 pKa = 11.84 NLSHH63 pKa = 7.34 TYY65 pKa = 9.06 TNCKK69 pKa = 9.8 GLPKK73 pKa = 10.06 RR74 pKa = 11.84 VIADD78 pKa = 3.65 NPKK81 pKa = 10.05 KK82 pKa = 10.65 VYY84 pKa = 8.98 PAKK87 pKa = 10.06 ATEE90 pKa = 4.07 KK91 pKa = 10.54 CKK93 pKa = 10.31 PNHH96 pKa = 6.46 FKK98 pKa = 11.47 VMMFVAPGNKK108 pKa = 8.85 RR109 pKa = 11.84 NYY111 pKa = 8.73 FRR113 pKa = 11.84 QGDD116 pKa = 3.4 FHH118 pKa = 7.36 FYY120 pKa = 9.04 KK121 pKa = 10.18 QHH123 pKa = 5.71 GAIEE127 pKa = 4.27 YY128 pKa = 9.73 KK129 pKa = 10.13 VKK131 pKa = 10.63 KK132 pKa = 9.98 GNTYY136 pKa = 10.52 EE137 pKa = 4.49 SIAKK141 pKa = 9.17 FFKK144 pKa = 10.98 VPVTRR149 pKa = 11.84 VKK151 pKa = 10.72 RR152 pKa = 11.84 SGKK155 pKa = 9.52 LVPGRR160 pKa = 11.84 LLKK163 pKa = 10.82 FKK165 pKa = 11.24 ANVFSHH171 pKa = 5.52 KK172 pKa = 10.17 RR173 pKa = 11.84 GWATGPLLVDD183 pKa = 4.25 AKK185 pKa = 10.56 GKK187 pKa = 10.38 SIQDD191 pKa = 3.18 PRR193 pKa = 11.84 TASRR197 pKa = 11.84 DD198 pKa = 3.81 YY199 pKa = 11.13 PGLNYY204 pKa = 10.09 QKK206 pKa = 9.93 YY207 pKa = 9.78 CSSFCVKK214 pKa = 10.35 NRR216 pKa = 11.84 GIKK219 pKa = 9.79 VGHH222 pKa = 5.4 THH224 pKa = 6.57 PKK226 pKa = 9.06 VVKK229 pKa = 9.02 KK230 pKa = 9.01 TRR232 pKa = 3.2
Molecular weight: 26.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.256
IPC2_protein 9.823
IPC_protein 9.955
Toseland 10.555
ProMoST 10.16
Dawson 10.672
Bjellqvist 10.292
Wikipedia 10.804
Rodwell 11.272
Grimsley 10.716
Solomon 10.687
Lehninger 10.672
Nozaki 10.526
DTASelect 10.292
Thurlkill 10.54
EMBOSS 10.921
Sillero 10.584
Patrickios 10.965
IPC_peptide 10.701
IPC2_peptide 9.019
IPC2.peptide.svr19 8.639
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
203
0
203
62473
65
5688
307.7
34.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.327 ± 0.261
1.353 ± 0.121
5.729 ± 0.145
6.111 ± 0.406
4.139 ± 0.134
7.11 ± 0.603
1.881 ± 0.072
6.707 ± 0.208
7.672 ± 0.536
7.238 ± 0.275
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.601 ± 0.175
6.259 ± 0.239
4.039 ± 0.212
2.969 ± 0.102
3.906 ± 0.253
7.672 ± 0.462
7.541 ± 0.495
6.084 ± 0.129
1.111 ± 0.076
4.551 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here