Beihai sobemo-like virus 14

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 5.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEE5|A0A1L3KEE5_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 14 OX=1922685 PE=4 SV=1
MM1 pKa = 7.03VGYY4 pKa = 10.24RR5 pKa = 11.84GNRR8 pKa = 11.84VEE10 pKa = 4.47DD11 pKa = 3.42LRR13 pKa = 11.84LAVLLRR19 pKa = 11.84LEE21 pKa = 4.29EE22 pKa = 4.12LQEE25 pKa = 4.24GKK27 pKa = 10.5ALNDD31 pKa = 3.0VFSFIKK37 pKa = 10.45QEE39 pKa = 3.77PHH41 pKa = 6.5KK42 pKa = 10.28LAKK45 pKa = 10.28AEE47 pKa = 3.81QSMWRR52 pKa = 11.84LISGLSCTDD61 pKa = 3.16QLVATLLSEE70 pKa = 4.33EE71 pKa = 3.91LMAYY75 pKa = 9.88VVDD78 pKa = 4.86HH79 pKa = 6.64PMITNMGIGWGFAMEE94 pKa = 5.34GGVSWLRR101 pKa = 11.84NFMPSNPVAADD112 pKa = 3.29KK113 pKa = 11.31SSWDD117 pKa = 3.23WTVQPWVYY125 pKa = 11.33DD126 pKa = 3.58CFLEE130 pKa = 5.0IMLWLHH136 pKa = 7.47DD137 pKa = 4.85DD138 pKa = 4.92FSTCKK143 pKa = 10.33QKK145 pKa = 10.46ILRR148 pKa = 11.84NHH150 pKa = 6.28ILSVCEE156 pKa = 3.88EE157 pKa = 4.27KK158 pKa = 11.23VFDD161 pKa = 3.86TGGGRR166 pKa = 11.84LKK168 pKa = 10.59QPAPGIVASGWKK180 pKa = 7.2WTILFNCFGQLFLHH194 pKa = 6.62YY195 pKa = 10.12YY196 pKa = 10.21ADD198 pKa = 3.84PEE200 pKa = 4.6GRR202 pKa = 11.84FPPPLTMGDD211 pKa = 3.47DD212 pKa = 4.71TIQEE216 pKa = 4.3EE217 pKa = 4.38TDD219 pKa = 3.26DD220 pKa = 5.23EE221 pKa = 4.8YY222 pKa = 10.86WCQIKK227 pKa = 8.43RR228 pKa = 11.84TGAILKK234 pKa = 8.42EE235 pKa = 4.32CQKK238 pKa = 10.97EE239 pKa = 3.9PIYY242 pKa = 10.99CSMRR246 pKa = 11.84LNDD249 pKa = 3.57WGFVPEE255 pKa = 5.08NMGKK259 pKa = 9.18YY260 pKa = 10.08CYY262 pKa = 10.2AFHH265 pKa = 7.28HH266 pKa = 6.9LDD268 pKa = 3.35EE269 pKa = 4.66EE270 pKa = 4.72LAVEE274 pKa = 4.52TLASYY279 pKa = 10.27QWLFAYY285 pKa = 9.78MPEE288 pKa = 3.74QCAAIQRR295 pKa = 11.84WLRR298 pKa = 11.84LLGGAHH304 pKa = 6.68FVTDD308 pKa = 3.69MLYY311 pKa = 8.71MQLYY315 pKa = 8.57MEE317 pKa = 4.86GRR319 pKa = 11.84TNEE322 pKa = 3.77WW323 pKa = 2.71

Molecular weight: 37.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEE5|A0A1L3KEE5_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 14 OX=1922685 PE=4 SV=1
MM1 pKa = 6.44WWARR5 pKa = 11.84SLYY8 pKa = 9.34LTIFGLIVQVSRR20 pKa = 11.84SGPSQSLVSFLEE32 pKa = 4.13TGSYY36 pKa = 10.15KK37 pKa = 10.81SFWKK41 pKa = 10.55VPGYY45 pKa = 10.1QEE47 pKa = 4.76VEE49 pKa = 4.01YY50 pKa = 10.1STDD53 pKa = 3.27NNTKK57 pKa = 9.19SVCSVGLMPEE67 pKa = 4.63GPRR70 pKa = 11.84GSFYY74 pKa = 10.98HH75 pKa = 6.78LGCATCVLTQVKK87 pKa = 9.98RR88 pKa = 11.84DD89 pKa = 3.44GVLADD94 pKa = 3.9EE95 pKa = 5.2FLTATHH101 pKa = 5.22VTEE104 pKa = 4.48DD105 pKa = 3.78QKK107 pKa = 11.86DD108 pKa = 3.76LYY110 pKa = 9.88VWRR113 pKa = 11.84NGRR116 pKa = 11.84VAKK119 pKa = 10.35VKK121 pKa = 9.93QFWQPAGQKK130 pKa = 9.8CFYY133 pKa = 9.9EE134 pKa = 4.27IMILRR139 pKa = 11.84VVSGASLLGCKK150 pKa = 8.72PAKK153 pKa = 9.36TVSSFKK159 pKa = 10.69DD160 pKa = 3.42DD161 pKa = 4.58NIVNLYY167 pKa = 10.29AAVEE171 pKa = 4.05GRR173 pKa = 11.84LIKK176 pKa = 8.65TTGTIASMGKK186 pKa = 9.52SAMLGIAHH194 pKa = 7.13TANTLPGWSGAGIFTPSGLVAFHH217 pKa = 6.03HH218 pKa = 6.54HH219 pKa = 6.9GIKK222 pKa = 10.23KK223 pKa = 8.72EE224 pKa = 3.96AKK226 pKa = 9.75DD227 pKa = 3.59GKK229 pKa = 10.38GCINTGYY236 pKa = 10.45EE237 pKa = 3.48IGSIINLLRR246 pKa = 11.84RR247 pKa = 11.84SAEE250 pKa = 3.75ARR252 pKa = 11.84PKK254 pKa = 10.38LNQLVTHH261 pKa = 7.12DD262 pKa = 4.14NQLSSEE268 pKa = 4.34CEE270 pKa = 4.84SVDD273 pKa = 2.83TWNWFKK279 pKa = 11.9DD280 pKa = 3.31MVEE283 pKa = 3.92AGKK286 pKa = 9.12MEE288 pKa = 4.19MTVTPWGDD296 pKa = 3.2EE297 pKa = 3.65QLYY300 pKa = 8.51TFTHH304 pKa = 5.66SGKK307 pKa = 9.58RR308 pKa = 11.84WMYY311 pKa = 10.53TSDD314 pKa = 3.85EE315 pKa = 4.49LPDD318 pKa = 4.55DD319 pKa = 4.23FLDD322 pKa = 4.96EE323 pKa = 4.3IEE325 pKa = 5.04EE326 pKa = 4.25YY327 pKa = 11.21ADD329 pKa = 4.42DD330 pKa = 4.26SLIDD334 pKa = 3.88EE335 pKa = 5.04EE336 pKa = 5.5DD337 pKa = 4.46DD338 pKa = 3.31YY339 pKa = 11.7HH340 pKa = 7.18QRR342 pKa = 11.84SRR344 pKa = 11.84GKK346 pKa = 8.83RR347 pKa = 11.84TSSRR351 pKa = 11.84YY352 pKa = 8.65RR353 pKa = 11.84DD354 pKa = 3.49EE355 pKa = 5.56LGRR358 pKa = 11.84CSDD361 pKa = 3.68DD362 pKa = 3.84DD363 pKa = 5.02GYY365 pKa = 11.98GEE367 pKa = 5.62APDD370 pKa = 4.89DD371 pKa = 5.09DD372 pKa = 4.41YY373 pKa = 12.04PEE375 pKa = 4.88IYY377 pKa = 10.49SDD379 pKa = 4.2FEE381 pKa = 4.5CVSSKK386 pKa = 11.06EE387 pKa = 4.01KK388 pKa = 10.62LDD390 pKa = 3.88PLGMPMTPIDD400 pKa = 4.11LEE402 pKa = 4.04AVEE405 pKa = 4.86ALRR408 pKa = 11.84EE409 pKa = 4.41AIDD412 pKa = 3.57EE413 pKa = 4.18VDD415 pKa = 3.12IQKK418 pKa = 11.17AKK420 pKa = 9.03VTAFLRR426 pKa = 11.84ALSEE430 pKa = 4.06AQIKK434 pKa = 9.54ARR436 pKa = 11.84RR437 pKa = 11.84AWEE440 pKa = 3.99ADD442 pKa = 3.2EE443 pKa = 4.54TDD445 pKa = 3.23AKK447 pKa = 10.89FDD449 pKa = 4.17VILQASADD457 pKa = 3.88IDD459 pKa = 3.92CLQAFCEE466 pKa = 4.06RR467 pKa = 11.84FAAEE471 pKa = 4.31RR472 pKa = 11.84EE473 pKa = 4.45SFTAKK478 pKa = 10.41YY479 pKa = 10.06NEE481 pKa = 3.95VMQKK485 pKa = 10.65SDD487 pKa = 3.24VDD489 pKa = 3.77STLDD493 pKa = 3.19RR494 pKa = 11.84RR495 pKa = 11.84VARR498 pKa = 11.84KK499 pKa = 9.86KK500 pKa = 10.28KK501 pKa = 10.28GKK503 pKa = 9.9EE504 pKa = 3.64KK505 pKa = 10.68LPPLPYY511 pKa = 10.06TSGLRR516 pKa = 11.84DD517 pKa = 3.53DD518 pKa = 4.46AVRR521 pKa = 11.84SIDD524 pKa = 3.29VCRR527 pKa = 11.84AVVDD531 pKa = 4.84SIPMRR536 pKa = 11.84NRR538 pKa = 11.84ASAAGVEE545 pKa = 4.59VQVSQPEE552 pKa = 4.1GLYY555 pKa = 9.45TDD557 pKa = 3.67EE558 pKa = 4.14GRR560 pKa = 11.84IVLAEE565 pKa = 4.03VYY567 pKa = 9.65GVKK570 pKa = 10.28VKK572 pKa = 11.01SDD574 pKa = 3.01IKK576 pKa = 10.79RR577 pKa = 11.84DD578 pKa = 3.2IKK580 pKa = 11.35GPLNYY585 pKa = 9.88KK586 pKa = 9.62GAPQRR591 pKa = 11.84LEE593 pKa = 3.78MSPDD597 pKa = 3.33GDD599 pKa = 3.91SQRR602 pKa = 11.84DD603 pKa = 3.42SSRR606 pKa = 11.84ASEE609 pKa = 4.2KK610 pKa = 9.7VQKK613 pKa = 10.64SSLITSTIQSKK624 pKa = 10.3EE625 pKa = 3.97NQEE628 pKa = 3.88NTQMSRR634 pKa = 11.84ISSGNCSQQQTNLLPPRR651 pKa = 11.84RR652 pKa = 11.84RR653 pKa = 11.84GTGEE657 pKa = 3.61RR658 pKa = 11.84LKK660 pKa = 10.8SHH662 pKa = 6.39SEE664 pKa = 4.3SILSEE669 pKa = 3.75HH670 pKa = 6.59GKK672 pKa = 9.32PVIAASHH679 pKa = 6.74LIEE682 pKa = 5.45LYY684 pKa = 10.42RR685 pKa = 11.84EE686 pKa = 4.09PLSKK690 pKa = 10.5EE691 pKa = 3.62FQSYY695 pKa = 9.8LRR697 pKa = 11.84EE698 pKa = 4.03PRR700 pKa = 11.84TVSSEE705 pKa = 3.62QEE707 pKa = 3.65RR708 pKa = 11.84RR709 pKa = 11.84KK710 pKa = 10.63KK711 pKa = 10.34KK712 pKa = 9.62IKK714 pKa = 10.05NVRR717 pKa = 11.84DD718 pKa = 3.43VANPRR723 pKa = 11.84ASSKK727 pKa = 11.0NFLSSAAPSVSRR739 pKa = 11.84AGFRR743 pKa = 11.84GPVANKK749 pKa = 9.92RR750 pKa = 11.84CC751 pKa = 3.52

Molecular weight: 83.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1074

323

751

537.0

60.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.076 ± 0.436

2.235 ± 0.425

6.518 ± 0.466

7.542 ± 0.098

3.445 ± 0.591

6.704 ± 0.205

1.862 ± 0.303

4.562 ± 0.112

6.145 ± 0.893

8.473 ± 1.317

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.793 ± 0.912

2.98 ± 0.057

4.283 ± 0.025

4.19 ± 0.224

6.145 ± 0.893

8.194 ± 1.902

4.842 ± 0.25

6.238 ± 0.481

2.328 ± 0.989

3.445 ± 0.286
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski