Amino acid dipepetide frequency for Geobacillus phage TP-84

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.06AlaAla: 4.06 ± 0.87
0.77AlaCys: 0.77 ± 0.305
4.621AlaAsp: 4.621 ± 0.591
4.271AlaGlu: 4.271 ± 0.542
2.31AlaPhe: 2.31 ± 0.403
3.99AlaGly: 3.99 ± 0.575
1.19AlaHis: 1.19 ± 0.3
6.091AlaIle: 6.091 ± 0.779
5.111AlaLys: 5.111 ± 0.656
5.251AlaLeu: 5.251 ± 0.603
2.45AlaMet: 2.45 ± 0.409
3.36AlaAsn: 3.36 ± 0.451
1.05AlaPro: 1.05 ± 0.292
2.94AlaGln: 2.94 ± 0.576
3.15AlaArg: 3.15 ± 0.599
2.24AlaSer: 2.24 ± 0.322
3.92AlaThr: 3.92 ± 0.833
4.271AlaVal: 4.271 ± 0.635
1.05AlaTrp: 1.05 ± 0.341
3.01AlaTyr: 3.01 ± 0.402
0.0AlaXaa: 0.0 ± 0.0
Cys
0.42CysAla: 0.42 ± 0.171
0.0CysCys: 0.0 ± 0.0
0.56CysAsp: 0.56 ± 0.183
0.98CysGlu: 0.98 ± 0.273
0.28CysPhe: 0.28 ± 0.138
0.56CysGly: 0.56 ± 0.244
0.14CysHis: 0.14 ± 0.101
0.14CysIle: 0.14 ± 0.099
0.56CysLys: 0.56 ± 0.178
0.56CysLeu: 0.56 ± 0.191
0.28CysMet: 0.28 ± 0.142
0.07CysAsn: 0.07 ± 0.064
0.42CysPro: 0.42 ± 0.225
0.35CysGln: 0.35 ± 0.16
0.42CysArg: 0.42 ± 0.195
0.07CysSer: 0.07 ± 0.081
0.42CysThr: 0.42 ± 0.165
0.42CysVal: 0.42 ± 0.184
0.0CysTrp: 0.0 ± 0.0
0.35CysTyr: 0.35 ± 0.207
0.0CysXaa: 0.0 ± 0.0
Asp
4.481AspAla: 4.481 ± 0.555
0.35AspCys: 0.35 ± 0.151
5.391AspAsp: 5.391 ± 0.828
5.671AspGlu: 5.671 ± 0.801
2.24AspPhe: 2.24 ± 0.32
4.621AspGly: 4.621 ± 0.71
2.03AspHis: 2.03 ± 0.436
3.78AspIle: 3.78 ± 0.547
3.71AspLys: 3.71 ± 0.589
3.92AspLeu: 3.92 ± 0.543
2.17AspMet: 2.17 ± 0.348
2.59AspAsn: 2.59 ± 0.439
4.201AspPro: 4.201 ± 0.661
2.8AspGln: 2.8 ± 0.431
3.99AspArg: 3.99 ± 0.558
1.68AspSer: 1.68 ± 0.265
2.52AspThr: 2.52 ± 0.472
3.71AspVal: 3.71 ± 0.437
1.19AspTrp: 1.19 ± 0.286
2.59AspTyr: 2.59 ± 0.488
0.0AspXaa: 0.0 ± 0.0
Glu
3.22GluAla: 3.22 ± 0.443
0.49GluCys: 0.49 ± 0.165
2.45GluAsp: 2.45 ± 0.44
5.461GluGlu: 5.461 ± 0.781
3.71GluPhe: 3.71 ± 0.476
3.99GluGly: 3.99 ± 0.589
1.47GluHis: 1.47 ± 0.412
5.181GluIle: 5.181 ± 0.715
6.441GluLys: 6.441 ± 1.186
7.001GluLeu: 7.001 ± 0.828
2.38GluMet: 2.38 ± 0.371
3.08GluAsn: 3.08 ± 0.499
1.96GluPro: 1.96 ± 0.412
3.92GluGln: 3.92 ± 0.651
4.551GluArg: 4.551 ± 0.589
3.08GluSer: 3.08 ± 0.403
4.761GluThr: 4.761 ± 0.53
3.85GluVal: 3.85 ± 0.493
2.17GluTrp: 2.17 ± 0.406
2.94GluTyr: 2.94 ± 0.572
0.0GluXaa: 0.0 ± 0.0
Phe
1.75PheAla: 1.75 ± 0.354
0.28PheCys: 0.28 ± 0.156
2.87PheAsp: 2.87 ± 0.451
2.8PheGlu: 2.8 ± 0.49
1.47PhePhe: 1.47 ± 0.279
2.66PheGly: 2.66 ± 0.36
1.05PheHis: 1.05 ± 0.282
3.15PheIle: 3.15 ± 0.494
2.45PheLys: 2.45 ± 0.375
2.59PheLeu: 2.59 ± 0.337
1.26PheMet: 1.26 ± 0.255
1.89PheAsn: 1.89 ± 0.335
1.05PhePro: 1.05 ± 0.281
1.75PheGln: 1.75 ± 0.311
0.98PheArg: 0.98 ± 0.258
1.54PheSer: 1.54 ± 0.333
2.87PheThr: 2.87 ± 0.49
1.89PheVal: 1.89 ± 0.348
0.56PheTrp: 0.56 ± 0.298
1.61PheTyr: 1.61 ± 0.266
0.0PheXaa: 0.0 ± 0.0
Gly
3.43GlyAla: 3.43 ± 0.509
0.49GlyCys: 0.49 ± 0.178
4.201GlyAsp: 4.201 ± 0.533
4.761GlyGlu: 4.761 ± 0.673
3.43GlyPhe: 3.43 ± 0.568
5.251GlyGly: 5.251 ± 0.598
0.91GlyHis: 0.91 ± 0.276
6.161GlyIle: 6.161 ± 0.646
4.341GlyLys: 4.341 ± 0.47
5.111GlyLeu: 5.111 ± 0.647
2.94GlyMet: 2.94 ± 0.414
3.01GlyAsn: 3.01 ± 0.31
2.17GlyPro: 2.17 ± 0.453
4.201GlyGln: 4.201 ± 0.556
3.29GlyArg: 3.29 ± 0.53
3.43GlySer: 3.43 ± 0.738
4.971GlyThr: 4.971 ± 0.621
4.761GlyVal: 4.761 ± 0.521
1.19GlyTrp: 1.19 ± 0.265
2.45GlyTyr: 2.45 ± 0.461
0.0GlyXaa: 0.0 ± 0.0
His
1.61HisAla: 1.61 ± 0.26
0.14HisCys: 0.14 ± 0.092
0.91HisAsp: 0.91 ± 0.26
1.26HisGlu: 1.26 ± 0.336
0.91HisPhe: 0.91 ± 0.253
1.12HisGly: 1.12 ± 0.288
0.56HisHis: 0.56 ± 0.164
1.89HisIle: 1.89 ± 0.394
1.4HisLys: 1.4 ± 0.274
1.26HisLeu: 1.26 ± 0.25
0.84HisMet: 0.84 ± 0.236
0.56HisAsn: 0.56 ± 0.185
1.26HisPro: 1.26 ± 0.241
1.05HisGln: 1.05 ± 0.248
0.91HisArg: 0.91 ± 0.252
1.19HisSer: 1.19 ± 0.254
0.98HisThr: 0.98 ± 0.272
1.68HisVal: 1.68 ± 0.34
0.28HisTrp: 0.28 ± 0.135
0.77HisTyr: 0.77 ± 0.199
0.0HisXaa: 0.0 ± 0.0
Ile
4.901IleAla: 4.901 ± 0.661
0.7IleCys: 0.7 ± 0.216
6.581IleAsp: 6.581 ± 0.593
7.351IleGlu: 7.351 ± 0.859
1.61IlePhe: 1.61 ± 0.289
5.461IleGly: 5.461 ± 0.61
1.75IleHis: 1.75 ± 0.325
4.341IleIle: 4.341 ± 0.495
7.071IleLys: 7.071 ± 0.922
3.92IleLeu: 3.92 ± 0.618
2.52IleMet: 2.52 ± 0.423
3.5IleAsn: 3.5 ± 0.504
3.01IlePro: 3.01 ± 0.353
3.92IleGln: 3.92 ± 0.523
4.201IleArg: 4.201 ± 0.615
3.29IleSer: 3.29 ± 0.654
5.181IleThr: 5.181 ± 0.667
4.971IleVal: 4.971 ± 0.782
0.91IleTrp: 0.91 ± 0.187
2.66IleTyr: 2.66 ± 0.451
0.0IleXaa: 0.0 ± 0.0
Lys
6.721LysAla: 6.721 ± 0.887
1.19LysCys: 1.19 ± 0.277
3.78LysAsp: 3.78 ± 0.392
4.341LysGlu: 4.341 ± 0.598
1.82LysPhe: 1.82 ± 0.393
5.461LysGly: 5.461 ± 0.704
1.68LysHis: 1.68 ± 0.354
5.251LysIle: 5.251 ± 0.523
7.001LysLys: 7.001 ± 0.917
5.181LysLeu: 5.181 ± 0.615
2.87LysMet: 2.87 ± 0.424
4.551LysAsn: 4.551 ± 0.781
3.08LysPro: 3.08 ± 0.46
4.13LysGln: 4.13 ± 0.613
5.741LysArg: 5.741 ± 0.764
3.36LysSer: 3.36 ± 0.708
5.811LysThr: 5.811 ± 0.683
4.201LysVal: 4.201 ± 0.764
1.75LysTrp: 1.75 ± 0.415
3.57LysTyr: 3.57 ± 0.614
0.0LysXaa: 0.0 ± 0.0
Leu
5.881LeuAla: 5.881 ± 0.665
0.42LeuCys: 0.42 ± 0.196
4.271LeuAsp: 4.271 ± 0.697
5.601LeuGlu: 5.601 ± 0.716
1.96LeuPhe: 1.96 ± 0.347
4.13LeuGly: 4.13 ± 0.525
0.77LeuHis: 0.77 ± 0.212
4.971LeuIle: 4.971 ± 0.652
7.071LeuLys: 7.071 ± 0.926
3.22LeuLeu: 3.22 ± 0.47
2.38LeuMet: 2.38 ± 0.368
2.87LeuAsn: 2.87 ± 0.516
2.66LeuPro: 2.66 ± 0.596
3.57LeuGln: 3.57 ± 0.537
2.03LeuArg: 2.03 ± 0.363
2.87LeuSer: 2.87 ± 0.388
3.71LeuThr: 3.71 ± 0.536
3.85LeuVal: 3.85 ± 0.54
0.91LeuTrp: 0.91 ± 0.303
2.03LeuTyr: 2.03 ± 0.364
0.0LeuXaa: 0.0 ± 0.0
Met
3.92MetAla: 3.92 ± 0.518
0.0MetCys: 0.0 ± 0.0
2.17MetAsp: 2.17 ± 0.38
2.17MetGlu: 2.17 ± 0.438
1.19MetPhe: 1.19 ± 0.292
2.31MetGly: 2.31 ± 0.393
0.56MetHis: 0.56 ± 0.151
2.94MetIle: 2.94 ± 0.413
3.43MetLys: 3.43 ± 0.537
1.75MetLeu: 1.75 ± 0.414
1.33MetMet: 1.33 ± 0.328
2.1MetAsn: 2.1 ± 0.324
1.54MetPro: 1.54 ± 0.353
1.68MetGln: 1.68 ± 0.28
1.33MetArg: 1.33 ± 0.323
1.54MetSer: 1.54 ± 0.341
2.38MetThr: 2.38 ± 0.358
2.31MetVal: 2.31 ± 0.394
0.56MetTrp: 0.56 ± 0.188
0.63MetTyr: 0.63 ± 0.235
0.0MetXaa: 0.0 ± 0.0
Asn
4.13AsnAla: 4.13 ± 0.584
0.28AsnCys: 0.28 ± 0.131
2.8AsnAsp: 2.8 ± 0.391
2.87AsnGlu: 2.87 ± 0.634
1.19AsnPhe: 1.19 ± 0.319
5.251AsnGly: 5.251 ± 0.633
0.77AsnHis: 0.77 ± 0.215
3.57AsnIle: 3.57 ± 0.74
3.85AsnLys: 3.85 ± 0.44
3.85AsnLeu: 3.85 ± 0.551
1.54AsnMet: 1.54 ± 0.383
1.89AsnAsn: 1.89 ± 0.363
2.59AsnPro: 2.59 ± 0.58
1.68AsnGln: 1.68 ± 0.275
1.75AsnArg: 1.75 ± 0.338
1.33AsnSer: 1.33 ± 0.298
1.68AsnThr: 1.68 ± 0.41
2.8AsnVal: 2.8 ± 0.483
0.98AsnTrp: 0.98 ± 0.245
1.75AsnTyr: 1.75 ± 0.348
0.0AsnXaa: 0.0 ± 0.0
Pro
1.19ProAla: 1.19 ± 0.268
0.07ProCys: 0.07 ± 0.066
2.38ProAsp: 2.38 ± 0.427
3.08ProGlu: 3.08 ± 0.466
1.89ProPhe: 1.89 ± 0.402
3.71ProGly: 3.71 ± 0.605
1.05ProHis: 1.05 ± 0.311
2.59ProIle: 2.59 ± 0.468
3.92ProLys: 3.92 ± 0.62
2.03ProLeu: 2.03 ± 0.34
1.4ProMet: 1.4 ± 0.274
2.59ProAsn: 2.59 ± 0.473
2.17ProPro: 2.17 ± 0.398
1.61ProGln: 1.61 ± 0.296
2.03ProArg: 2.03 ± 0.416
2.1ProSer: 2.1 ± 0.373
1.89ProThr: 1.89 ± 0.459
2.24ProVal: 2.24 ± 0.35
0.14ProTrp: 0.14 ± 0.08
1.26ProTyr: 1.26 ± 0.286
0.0ProXaa: 0.0 ± 0.0
Gln
3.22GlnAla: 3.22 ± 0.525
0.07GlnCys: 0.07 ± 0.055
2.45GlnAsp: 2.45 ± 0.385
3.15GlnGlu: 3.15 ± 0.536
1.82GlnPhe: 1.82 ± 0.305
3.57GlnGly: 3.57 ± 0.465
0.63GlnHis: 0.63 ± 0.239
3.78GlnIle: 3.78 ± 0.466
4.271GlnLys: 4.271 ± 0.686
1.96GlnLeu: 1.96 ± 0.426
1.96GlnMet: 1.96 ± 0.335
2.8GlnAsn: 2.8 ± 0.374
1.54GlnPro: 1.54 ± 0.433
3.01GlnGln: 3.01 ± 0.77
2.52GlnArg: 2.52 ± 0.441
2.1GlnSer: 2.1 ± 0.372
3.22GlnThr: 3.22 ± 0.487
2.73GlnVal: 2.73 ± 0.497
0.91GlnTrp: 0.91 ± 0.205
1.96GlnTyr: 1.96 ± 0.306
0.0GlnXaa: 0.0 ± 0.0
Arg
2.24ArgAla: 2.24 ± 0.33
0.35ArgCys: 0.35 ± 0.162
2.73ArgAsp: 2.73 ± 0.427
3.15ArgGlu: 3.15 ± 0.394
2.8ArgPhe: 2.8 ± 0.498
2.73ArgGly: 2.73 ± 0.51
1.19ArgHis: 1.19 ± 0.303
4.341ArgIle: 4.341 ± 0.682
5.111ArgLys: 5.111 ± 0.707
3.85ArgLeu: 3.85 ± 0.512
1.96ArgMet: 1.96 ± 0.358
1.89ArgAsn: 1.89 ± 0.469
1.54ArgPro: 1.54 ± 0.353
2.24ArgGln: 2.24 ± 0.488
2.52ArgArg: 2.52 ± 0.492
2.94ArgSer: 2.94 ± 0.441
2.24ArgThr: 2.24 ± 0.473
2.59ArgVal: 2.59 ± 0.447
1.54ArgTrp: 1.54 ± 0.375
1.96ArgTyr: 1.96 ± 0.396
0.0ArgXaa: 0.0 ± 0.0
Ser
2.87SerAla: 2.87 ± 0.6
0.21SerCys: 0.21 ± 0.111
2.8SerAsp: 2.8 ± 0.495
2.66SerGlu: 2.66 ± 0.33
1.96SerPhe: 1.96 ± 0.312
4.691SerGly: 4.691 ± 0.517
0.77SerHis: 0.77 ± 0.213
2.59SerIle: 2.59 ± 0.364
3.22SerLys: 3.22 ± 0.555
3.01SerLeu: 3.01 ± 0.458
1.61SerMet: 1.61 ± 0.349
1.89SerAsn: 1.89 ± 0.426
1.4SerPro: 1.4 ± 0.225
1.68SerGln: 1.68 ± 0.363
2.94SerArg: 2.94 ± 0.442
1.12SerSer: 1.12 ± 0.217
2.59SerThr: 2.59 ± 0.375
2.8SerVal: 2.8 ± 0.397
0.63SerTrp: 0.63 ± 0.203
1.54SerTyr: 1.54 ± 0.297
0.0SerXaa: 0.0 ± 0.0
Thr
3.85ThrAla: 3.85 ± 0.55
0.42ThrCys: 0.42 ± 0.208
4.411ThrAsp: 4.411 ± 0.71
2.87ThrGlu: 2.87 ± 0.482
2.1ThrPhe: 2.1 ± 0.391
4.201ThrGly: 4.201 ± 0.542
1.26ThrHis: 1.26 ± 0.24
7.071ThrIle: 7.071 ± 1.012
3.57ThrLys: 3.57 ± 0.562
4.06ThrLeu: 4.06 ± 0.467
2.03ThrMet: 2.03 ± 0.35
2.1ThrAsn: 2.1 ± 0.328
3.15ThrPro: 3.15 ± 0.462
1.96ThrGln: 1.96 ± 0.429
2.52ThrArg: 2.52 ± 0.355
3.43ThrSer: 3.43 ± 0.395
3.36ThrThr: 3.36 ± 0.486
4.621ThrVal: 4.621 ± 0.549
0.84ThrTrp: 0.84 ± 0.214
2.59ThrTyr: 2.59 ± 0.577
0.0ThrXaa: 0.0 ± 0.0
Val
4.06ValAla: 4.06 ± 0.506
0.21ValCys: 0.21 ± 0.126
5.251ValAsp: 5.251 ± 0.661
4.901ValGlu: 4.901 ± 0.738
1.75ValPhe: 1.75 ± 0.308
4.06ValGly: 4.06 ± 0.575
1.12ValHis: 1.12 ± 0.271
5.671ValIle: 5.671 ± 0.768
4.551ValLys: 4.551 ± 0.557
3.22ValLeu: 3.22 ± 0.582
2.38ValMet: 2.38 ± 0.427
3.15ValAsn: 3.15 ± 0.512
2.38ValPro: 2.38 ± 0.374
2.45ValGln: 2.45 ± 0.497
1.68ValArg: 1.68 ± 0.36
2.59ValSer: 2.59 ± 0.421
3.64ValThr: 3.64 ± 0.445
3.5ValVal: 3.5 ± 0.435
0.91ValTrp: 0.91 ± 0.254
3.22ValTyr: 3.22 ± 0.594
0.0ValXaa: 0.0 ± 0.0
Trp
0.91TrpAla: 0.91 ± 0.281
0.14TrpCys: 0.14 ± 0.092
0.84TrpAsp: 0.84 ± 0.227
2.03TrpGlu: 2.03 ± 0.407
0.35TrpPhe: 0.35 ± 0.162
0.56TrpGly: 0.56 ± 0.222
0.21TrpHis: 0.21 ± 0.102
1.75TrpIle: 1.75 ± 0.397
1.47TrpLys: 1.47 ± 0.42
0.63TrpLeu: 0.63 ± 0.225
0.56TrpMet: 0.56 ± 0.175
0.77TrpAsn: 0.77 ± 0.242
0.84TrpPro: 0.84 ± 0.304
0.77TrpGln: 0.77 ± 0.231
0.84TrpArg: 0.84 ± 0.21
1.12TrpSer: 1.12 ± 0.262
1.68TrpThr: 1.68 ± 0.304
1.05TrpVal: 1.05 ± 0.256
0.14TrpTrp: 0.14 ± 0.097
0.7TrpTyr: 0.7 ± 0.195
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.24TyrAla: 2.24 ± 0.485
0.42TyrCys: 0.42 ± 0.238
2.17TyrAsp: 2.17 ± 0.427
2.73TyrGlu: 2.73 ± 0.465
1.68TyrPhe: 1.68 ± 0.385
2.24TyrGly: 2.24 ± 0.401
1.47TyrHis: 1.47 ± 0.309
2.59TyrIle: 2.59 ± 0.522
2.94TyrLys: 2.94 ± 0.462
2.8TyrLeu: 2.8 ± 0.394
0.91TyrMet: 0.91 ± 0.261
1.89TyrAsn: 1.89 ± 0.419
1.33TyrPro: 1.33 ± 0.304
1.96TyrGln: 1.96 ± 0.325
2.52TyrArg: 2.52 ± 0.512
1.96TyrSer: 1.96 ± 0.383
2.59TyrThr: 2.59 ± 0.584
2.45TyrVal: 2.45 ± 0.462
0.7TyrTrp: 0.7 ± 0.242
1.68TyrTyr: 1.68 ± 0.365
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 79 proteins (14285 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski