Candidatus Pandoraea novymonadis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Pandoraea

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 967 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2P6UYC9|A0A2P6UYC9_9BURK Dihydroneopterin triphosphate diphosphatase OS=Candidatus Pandoraea novymonadis OX=1808959 GN=nudB PE=4 SV=1
MM1 pKa = 7.42MDD3 pKa = 3.2VSIEE7 pKa = 4.05ATVGEE12 pKa = 4.23IPIVFTDD19 pKa = 4.04SAAHH23 pKa = 5.4SVKK26 pKa = 10.53HH27 pKa = 6.09LIEE30 pKa = 4.48EE31 pKa = 4.35EE32 pKa = 4.35GNPEE36 pKa = 3.6LKK38 pKa = 10.63LRR40 pKa = 11.84VFIQGGGCSGFQYY53 pKa = 10.98GFTFDD58 pKa = 4.02EE59 pKa = 5.95DD60 pKa = 3.6INEE63 pKa = 4.89DD64 pKa = 3.58DD65 pKa = 4.33TVVDD69 pKa = 3.92KK70 pKa = 11.82NGVSLVIDD78 pKa = 3.86AMSYY82 pKa = 9.88QYY84 pKa = 11.5LVGAEE89 pKa = 3.83IDD91 pKa = 3.76YY92 pKa = 11.34KK93 pKa = 11.21EE94 pKa = 5.32DD95 pKa = 3.15INGAQFIIKK104 pKa = 9.82NPNASTTCGCGSSFSVV120 pKa = 3.54

Molecular weight:
12.97 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2P6UYL4|A0A2P6UYL4_9BURK Uncharacterized protein OS=Candidatus Pandoraea novymonadis OX=1808959 GN=BZL35_00653 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.43RR14 pKa = 11.84THH16 pKa = 5.88GFLVRR21 pKa = 11.84MKK23 pKa = 9.86TRR25 pKa = 11.84GGRR28 pKa = 11.84KK29 pKa = 8.94VISARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.07GRR39 pKa = 11.84HH40 pKa = 4.92RR41 pKa = 11.84LAVV44 pKa = 3.37

Molecular weight:
5.21 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

967

0

967

309465

31

1581

320.0

35.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.675 ± 0.084

1.162 ± 0.024

5.197 ± 0.049

5.784 ± 0.072

4.137 ± 0.056

7.164 ± 0.072

2.497 ± 0.037

6.921 ± 0.058

4.847 ± 0.066

10.594 ± 0.085

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.723 ± 0.037

3.766 ± 0.05

4.148 ± 0.039

3.601 ± 0.038

6.08 ± 0.055

6.212 ± 0.051

5.35 ± 0.055

7.25 ± 0.077

1.151 ± 0.032

2.741 ± 0.032

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski