Candidatus Pandoraea novymonadis
Average proteome isoelectric point is 6.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 967 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P6UYC9|A0A2P6UYC9_9BURK Dihydroneopterin triphosphate diphosphatase OS=Candidatus Pandoraea novymonadis OX=1808959 GN=nudB PE=4 SV=1
MM1 pKa = 7.42 MDD3 pKa = 3.2 VSIEE7 pKa = 4.05 ATVGEE12 pKa = 4.23 IPIVFTDD19 pKa = 4.04 SAAHH23 pKa = 5.4 SVKK26 pKa = 10.53 HH27 pKa = 6.09 LIEE30 pKa = 4.48 EE31 pKa = 4.35 EE32 pKa = 4.35 GNPEE36 pKa = 3.6 LKK38 pKa = 10.63 LRR40 pKa = 11.84 VFIQGGGCSGFQYY53 pKa = 10.98 GFTFDD58 pKa = 4.02 EE59 pKa = 5.95 DD60 pKa = 3.6 INEE63 pKa = 4.89 DD64 pKa = 3.58 DD65 pKa = 4.33 TVVDD69 pKa = 3.92 KK70 pKa = 11.82 NGVSLVIDD78 pKa = 3.86 AMSYY82 pKa = 9.88 QYY84 pKa = 11.5 LVGAEE89 pKa = 3.83 IDD91 pKa = 3.76 YY92 pKa = 11.34 KK93 pKa = 11.21 EE94 pKa = 5.32 DD95 pKa = 3.15 INGAQFIIKK104 pKa = 9.82 NPNASTTCGCGSSFSVV120 pKa = 3.54
Molecular weight: 12.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.802
IPC2_protein 4.024
IPC_protein 3.948
Toseland 3.757
ProMoST 4.101
Dawson 3.923
Bjellqvist 4.088
Wikipedia 3.846
Rodwell 3.783
Grimsley 3.681
Solomon 3.91
Lehninger 3.872
Nozaki 4.05
DTASelect 4.228
Thurlkill 3.808
EMBOSS 3.859
Sillero 4.075
Patrickios 1.914
IPC_peptide 3.923
IPC2_peptide 4.05
IPC2.peptide.svr19 3.954
Protein with the highest isoelectric point:
>tr|A0A2P6UYL4|A0A2P6UYL4_9BURK Uncharacterized protein OS=Candidatus Pandoraea novymonadis OX=1808959 GN=BZL35_00653 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 10.06 QPSVTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.43 RR14 pKa = 11.84 THH16 pKa = 5.88 GFLVRR21 pKa = 11.84 MKK23 pKa = 9.86 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 KK29 pKa = 8.94 VISARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.07 GRR39 pKa = 11.84 HH40 pKa = 4.92 RR41 pKa = 11.84 LAVV44 pKa = 3.37
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
967
0
967
309465
31
1581
320.0
35.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.675 ± 0.084
1.162 ± 0.024
5.197 ± 0.049
5.784 ± 0.072
4.137 ± 0.056
7.164 ± 0.072
2.497 ± 0.037
6.921 ± 0.058
4.847 ± 0.066
10.594 ± 0.085
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.723 ± 0.037
3.766 ± 0.05
4.148 ± 0.039
3.601 ± 0.038
6.08 ± 0.055
6.212 ± 0.051
5.35 ± 0.055
7.25 ± 0.077
1.151 ± 0.032
2.741 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here