Rhodoplanes sp. JGI PP 4-B12

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Hyphomicrobiaceae; Rhodoplanes; unclassified Rhodoplanes

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 220 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A212Q8N9|A0A212Q8N9_9RHIZ Probable addiction module antidote protein OS=Rhodoplanes sp. JGI PP 4-B12 OX=1873883 GN=SAMN05414138_1057 PE=4 SV=1
MM1 pKa = 7.52SKK3 pKa = 10.44PVIFTISAAWDD14 pKa = 3.54DD15 pKa = 4.27EE16 pKa = 4.48ASVWSGHH23 pKa = 6.64CDD25 pKa = 4.33DD26 pKa = 5.2IPAAADD32 pKa = 3.53APTLDD37 pKa = 3.95EE38 pKa = 4.82LLAKK42 pKa = 10.13ISAMALDD49 pKa = 5.03LSPDD53 pKa = 3.19NHH55 pKa = 6.77PEE57 pKa = 3.75VTAVALSLHH66 pKa = 5.29VAEE69 pKa = 4.95ASIDD73 pKa = 3.3RR74 pKa = 11.84SYY76 pKa = 11.99AA77 pKa = 3.17

Molecular weight:
8.11 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A212QJK6|A0A212QJK6_9RHIZ Trigger factor OS=Rhodoplanes sp. JGI PP 4-B12 OX=1873883 GN=tig PE=3 SV=1
MM1 pKa = 7.79AEE3 pKa = 4.55TIQTLEE9 pKa = 4.38EE10 pKa = 4.2LSSLKK15 pKa = 10.03PAAAPQPPQYY25 pKa = 10.2VQKK28 pKa = 10.72LDD30 pKa = 3.6KK31 pKa = 10.6QGRR34 pKa = 11.84AYY36 pKa = 10.09ATGKK40 pKa = 10.55RR41 pKa = 11.84KK42 pKa = 10.02DD43 pKa = 3.21AVARR47 pKa = 11.84VWIKK51 pKa = 9.9TGGGKK56 pKa = 9.21IVINDD61 pKa = 4.01RR62 pKa = 11.84PVEE65 pKa = 4.38SYY67 pKa = 9.95FARR70 pKa = 11.84PVLRR74 pKa = 11.84MMIQQPLVASNRR86 pKa = 11.84QGQYY90 pKa = 11.25DD91 pKa = 3.87VVCTVSGGGLSGQAGAVRR109 pKa = 11.84HH110 pKa = 5.71GLSKK114 pKa = 10.99ALLRR118 pKa = 11.84YY119 pKa = 9.73EE120 pKa = 4.32PDD122 pKa = 3.04LRR124 pKa = 11.84PVLKK128 pKa = 10.6RR129 pKa = 11.84GGFLTRR135 pKa = 11.84DD136 pKa = 3.39PRR138 pKa = 11.84VVEE141 pKa = 3.94RR142 pKa = 11.84KK143 pKa = 9.76KK144 pKa = 10.84YY145 pKa = 10.0GRR147 pKa = 11.84AKK149 pKa = 10.4ARR151 pKa = 11.84RR152 pKa = 11.84SFQFSKK158 pKa = 10.92RR159 pKa = 3.55

Molecular weight:
17.69 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

220

0

220

57497

34

1056

261.4

28.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.592 ± 0.206

0.948 ± 0.072

5.652 ± 0.118

5.439 ± 0.137

3.677 ± 0.111

8.068 ± 0.166

2.108 ± 0.074

5.301 ± 0.112

3.682 ± 0.123

9.573 ± 0.178

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.275 ± 0.068

2.847 ± 0.082

5.652 ± 0.146

3.4 ± 0.097

7.48 ± 0.168

5.176 ± 0.11

5.134 ± 0.107

7.127 ± 0.139

1.374 ± 0.076

2.494 ± 0.085

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski