Rhodoplanes sp. JGI PP 4-B12
Average proteome isoelectric point is 6.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 220 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A212Q8N9|A0A212Q8N9_9RHIZ Probable addiction module antidote protein OS=Rhodoplanes sp. JGI PP 4-B12 OX=1873883 GN=SAMN05414138_1057 PE=4 SV=1
MM1 pKa = 7.52 SKK3 pKa = 10.44 PVIFTISAAWDD14 pKa = 3.54 DD15 pKa = 4.27 EE16 pKa = 4.48 ASVWSGHH23 pKa = 6.64 CDD25 pKa = 4.33 DD26 pKa = 5.2 IPAAADD32 pKa = 3.53 APTLDD37 pKa = 3.95 EE38 pKa = 4.82 LLAKK42 pKa = 10.13 ISAMALDD49 pKa = 5.03 LSPDD53 pKa = 3.19 NHH55 pKa = 6.77 PEE57 pKa = 3.75 VTAVALSLHH66 pKa = 5.29 VAEE69 pKa = 4.95 ASIDD73 pKa = 3.3 RR74 pKa = 11.84 SYY76 pKa = 11.99 AA77 pKa = 3.17
Molecular weight: 8.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.162
IPC2_protein 4.037
IPC_protein 3.961
Toseland 3.757
ProMoST 4.151
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.923
Rodwell 3.795
Grimsley 3.668
Solomon 3.948
Lehninger 3.91
Nozaki 4.101
DTASelect 4.342
Thurlkill 3.834
EMBOSS 3.935
Sillero 4.088
Patrickios 3.427
IPC_peptide 3.948
IPC2_peptide 4.062
IPC2.peptide.svr19 3.998
Protein with the highest isoelectric point:
>tr|A0A212QJK6|A0A212QJK6_9RHIZ Trigger factor OS=Rhodoplanes sp. JGI PP 4-B12 OX=1873883 GN=tig PE=3 SV=1
MM1 pKa = 7.79 AEE3 pKa = 4.55 TIQTLEE9 pKa = 4.38 EE10 pKa = 4.2 LSSLKK15 pKa = 10.03 PAAAPQPPQYY25 pKa = 10.2 VQKK28 pKa = 10.72 LDD30 pKa = 3.6 KK31 pKa = 10.6 QGRR34 pKa = 11.84 AYY36 pKa = 10.09 ATGKK40 pKa = 10.55 RR41 pKa = 11.84 KK42 pKa = 10.02 DD43 pKa = 3.21 AVARR47 pKa = 11.84 VWIKK51 pKa = 9.9 TGGGKK56 pKa = 9.21 IVINDD61 pKa = 4.01 RR62 pKa = 11.84 PVEE65 pKa = 4.38 SYY67 pKa = 9.95 FARR70 pKa = 11.84 PVLRR74 pKa = 11.84 MMIQQPLVASNRR86 pKa = 11.84 QGQYY90 pKa = 11.25 DD91 pKa = 3.87 VVCTVSGGGLSGQAGAVRR109 pKa = 11.84 HH110 pKa = 5.71 GLSKK114 pKa = 10.99 ALLRR118 pKa = 11.84 YY119 pKa = 9.73 EE120 pKa = 4.32 PDD122 pKa = 3.04 LRR124 pKa = 11.84 PVLKK128 pKa = 10.6 RR129 pKa = 11.84 GGFLTRR135 pKa = 11.84 DD136 pKa = 3.39 PRR138 pKa = 11.84 VVEE141 pKa = 3.94 RR142 pKa = 11.84 KK143 pKa = 9.76 KK144 pKa = 10.84 YY145 pKa = 10.0 GRR147 pKa = 11.84 AKK149 pKa = 10.4 ARR151 pKa = 11.84 RR152 pKa = 11.84 SFQFSKK158 pKa = 10.92 RR159 pKa = 3.55
Molecular weight: 17.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.322
IPC2_protein 9.926
IPC_protein 10.789
Toseland 10.935
ProMoST 10.862
Dawson 11.023
Bjellqvist 10.745
Wikipedia 11.242
Rodwell 11.257
Grimsley 11.052
Solomon 11.169
Lehninger 11.125
Nozaki 10.921
DTASelect 10.745
Thurlkill 10.935
EMBOSS 11.345
Sillero 10.95
Patrickios 10.979
IPC_peptide 11.169
IPC2_peptide 9.648
IPC2.peptide.svr19 8.499
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
220
0
220
57497
34
1056
261.4
28.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.592 ± 0.206
0.948 ± 0.072
5.652 ± 0.118
5.439 ± 0.137
3.677 ± 0.111
8.068 ± 0.166
2.108 ± 0.074
5.301 ± 0.112
3.682 ± 0.123
9.573 ± 0.178
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.275 ± 0.068
2.847 ± 0.082
5.652 ± 0.146
3.4 ± 0.097
7.48 ± 0.168
5.176 ± 0.11
5.134 ± 0.107
7.127 ± 0.139
1.374 ± 0.076
2.494 ± 0.085
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here