Labrus bergylta (ballan wrasse)
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 37793 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3Q3FBZ3|A0A3Q3FBZ3_9LABR Dual-specificity kinase OS=Labrus bergylta OX=56723 PE=3 SV=1
MM1 pKa = 7.37 VLLKK5 pKa = 10.65 SLNPPTEE12 pKa = 4.35 GVRR15 pKa = 11.84 HH16 pKa = 5.28 EE17 pKa = 4.28 QAEE20 pKa = 4.53 TTAVMGGQRR29 pKa = 11.84 LGCGTLYY36 pKa = 10.64 VAEE39 pKa = 4.69 TRR41 pKa = 11.84 LSWFDD46 pKa = 3.26 GAGLGFCLEE55 pKa = 4.24 YY56 pKa = 9.14 PTIGLHH62 pKa = 6.67 AISRR66 pKa = 11.84 DD67 pKa = 3.15 VSAYY71 pKa = 8.46 PQEE74 pKa = 4.28 HH75 pKa = 7.06 LYY77 pKa = 11.6 VMVNGKK83 pKa = 9.81 VGDD86 pKa = 3.92 EE87 pKa = 4.34 NDD89 pKa = 3.18 AEE91 pKa = 4.37 MAEE94 pKa = 4.3 KK95 pKa = 10.41 AADD98 pKa = 3.81 EE99 pKa = 5.86 DD100 pKa = 4.18 EE101 pKa = 6.12 DD102 pKa = 5.61 DD103 pKa = 5.77 DD104 pKa = 4.95 NSCSSSGGDD113 pKa = 3.62 DD114 pKa = 4.25 DD115 pKa = 5.24 EE116 pKa = 5.55 EE117 pKa = 4.48 GTITEE122 pKa = 4.03 IRR124 pKa = 11.84 FVPSDD129 pKa = 3.45 KK130 pKa = 11.05 AALEE134 pKa = 4.1 PMFSAMCEE142 pKa = 4.24 CQALHH147 pKa = 7.42 PDD149 pKa = 4.21 PEE151 pKa = 6.72 DD152 pKa = 4.81 DD153 pKa = 5.87 DD154 pKa = 4.92 SDD156 pKa = 3.91 NDD158 pKa = 4.08 FEE160 pKa = 4.91 GEE162 pKa = 4.09 EE163 pKa = 4.31 YY164 pKa = 10.54 DD165 pKa = 4.73 VEE167 pKa = 4.32 EE168 pKa = 4.87 ADD170 pKa = 4.21 PEE172 pKa = 4.56 HH173 pKa = 6.32 GHH175 pKa = 6.69 GDD177 pKa = 2.8 IPTFYY182 pKa = 10.1 TCDD185 pKa = 3.45 EE186 pKa = 4.52 GLSSLTQEE194 pKa = 4.36 GQATLEE200 pKa = 4.09 RR201 pKa = 11.84 LEE203 pKa = 4.74 GMLAQSVAHH212 pKa = 5.87 QYY214 pKa = 11.26 NMAGVRR220 pKa = 11.84 TAEE223 pKa = 4.22 TNNEE227 pKa = 4.07 FEE229 pKa = 5.98 DD230 pKa = 4.18 GMEE233 pKa = 3.74 VDD235 pKa = 4.04 AAAMEE240 pKa = 4.63 AGQFEE245 pKa = 4.63 DD246 pKa = 5.03 ADD248 pKa = 3.96 VEE250 pKa = 4.5 HH251 pKa = 7.13
Molecular weight: 27.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.71
IPC2_protein 3.808
IPC_protein 3.795
Toseland 3.605
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.668
Rodwell 3.63
Grimsley 3.503
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.062
Thurlkill 3.63
EMBOSS 3.681
Sillero 3.91
Patrickios 1.265
IPC_peptide 3.757
IPC2_peptide 3.897
IPC2.peptide.svr19 3.808
Protein with the highest isoelectric point:
>tr|A0A3Q3M3U4|A0A3Q3M3U4_9LABR Peptidase S1 domain-containing protein OS=Labrus bergylta OX=56723 PE=4 SV=1
WW1 pKa = 6.94 SRR3 pKa = 11.84 SISLSWSRR11 pKa = 11.84 SISLSWSRR19 pKa = 11.84 SISLSWSRR27 pKa = 11.84 SLSLPWSRR35 pKa = 11.84 SISLSWSRR43 pKa = 11.84 SISLPWSRR51 pKa = 11.84 SVSLSWSRR59 pKa = 11.84 SVSLSWSRR67 pKa = 11.84 SISLPWSRR75 pKa = 11.84 SISLPWSRR83 pKa = 11.84 SVSLPWSRR91 pKa = 11.84 SISLPWSRR99 pKa = 11.84 SISLPWSRR107 pKa = 11.84 SISLPWSRR115 pKa = 11.84 SISLPWSRR123 pKa = 11.84 SVSLPWSRR131 pKa = 11.84 SISLPWSRR139 pKa = 11.84 SISLPWSRR147 pKa = 11.84 SISLPWSRR155 pKa = 11.84 SISLPWSRR163 pKa = 11.84 SISLPWSRR171 pKa = 11.84 SISLPWSRR179 pKa = 11.84 SISLPWSRR187 pKa = 11.84 SISLPWSRR195 pKa = 11.84 SLSLPWSRR203 pKa = 11.84 SLSLPWSRR211 pKa = 11.84 SLSLPWSRR219 pKa = 11.84 SISLPWSRR227 pKa = 11.84 SISLPWSRR235 pKa = 11.84 SISLPWSRR243 pKa = 11.84 SISLPWSRR251 pKa = 11.84 SISLPWSRR259 pKa = 11.84 SISLPWSRR267 pKa = 11.84 SVSLPWSRR275 pKa = 11.84 SVSLPWSRR283 pKa = 11.84 SVSLPWSRR291 pKa = 11.84 SISLPWSRR299 pKa = 11.84 SISLPWSRR307 pKa = 11.84 SVSLPWSRR315 pKa = 11.84 SISLPWSRR323 pKa = 11.84 SISLPWSRR331 pKa = 11.84 SISLPWSRR339 pKa = 11.84 SVSLPWSRR347 pKa = 11.84 SVSLPWSRR355 pKa = 11.84 SLSLPWSRR363 pKa = 11.84 SISLPWSRR371 pKa = 11.84 SISLPWSRR379 pKa = 11.84 SVSLPWSRR387 pKa = 11.84 SISLPWSRR395 pKa = 11.84 SLSLPWSRR403 pKa = 11.84 SLSLPWSRR411 pKa = 11.84 SVSLPWSRR419 pKa = 11.84 SVSLPWSRR427 pKa = 11.84 SISLPWSRR435 pKa = 11.84 SLSLPWSRR443 pKa = 11.84 SVSLPWSRR451 pKa = 11.84 SISLPWSRR459 pKa = 11.84 SLSLPWSRR467 pKa = 11.84 SVSLPCAPSEE477 pKa = 4.2 RR478 pKa = 11.84 EE479 pKa = 3.93 AGRR482 pKa = 11.84 LTLGLHH488 pKa = 5.03 SVQRR492 pKa = 11.84 VVSSGLRR499 pKa = 11.84 WNII502 pKa = 3.28
Molecular weight: 57.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.521
IPC2_protein 11.389
IPC_protein 13.013
Toseland 13.173
ProMoST 13.671
Dawson 13.173
Bjellqvist 13.173
Wikipedia 13.642
Rodwell 12.676
Grimsley 13.203
Solomon 13.671
Lehninger 13.568
Nozaki 13.173
DTASelect 13.173
Thurlkill 13.173
EMBOSS 13.671
Sillero 13.173
Patrickios 12.369
IPC_peptide 13.671
IPC2_peptide 12.661
IPC2.peptide.svr19 9.313
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
26930
10863
37793
19526510
15
8657
516.7
57.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.325 ± 0.011
2.364 ± 0.012
5.199 ± 0.009
6.765 ± 0.016
3.748 ± 0.01
6.24 ± 0.014
2.697 ± 0.008
4.415 ± 0.01
5.639 ± 0.014
9.635 ± 0.017
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.399 ± 0.006
3.89 ± 0.008
5.544 ± 0.016
4.671 ± 0.014
5.599 ± 0.011
8.772 ± 0.015
5.726 ± 0.011
6.418 ± 0.012
1.174 ± 0.004
2.779 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here