Acetohalobium arabaticum (strain ATCC 49924 / DSM 5501 / Z-7288)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Halanaerobiales; Halobacteroidaceae; Acetohalobium; Acetohalobium arabaticum

Average proteome isoelectric point is 5.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2276 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D9QSU2|D9QSU2_ACEAZ Ribonuclease J OS=Acetohalobium arabaticum (strain ATCC 49924 / DSM 5501 / Z-7288) OX=574087 GN=rnj PE=3 SV=1
MM1 pKa = 7.66SKK3 pKa = 10.78FNLQEE8 pKa = 4.17KK9 pKa = 10.23NNSNVNGNDD18 pKa = 3.32FAVEE22 pKa = 4.35VPFCCVQVVPGNLEE36 pKa = 3.98VVEE39 pKa = 4.76DD40 pKa = 3.86RR41 pKa = 11.84LEE43 pKa = 5.35AEE45 pKa = 4.29DD46 pKa = 5.1LKK48 pKa = 9.59MTFTPKK54 pKa = 10.5LEE56 pKa = 4.2CCIKK60 pKa = 10.85EE61 pKa = 4.03EE62 pKa = 4.43DD63 pKa = 4.14LSCIADD69 pKa = 3.7NVSCSIDD76 pKa = 3.72GEE78 pKa = 4.36VLRR81 pKa = 11.84LVGCIEE87 pKa = 4.11YY88 pKa = 10.41AISTINGTPDD98 pKa = 3.31PVRR101 pKa = 11.84GDD103 pKa = 4.02FSTEE107 pKa = 3.42APQQNSADD115 pKa = 3.9VSCKK119 pKa = 9.61STVCVNEE126 pKa = 4.81IICCGQDD133 pKa = 3.79LEE135 pKa = 5.54CPDD138 pKa = 5.51DD139 pKa = 5.02LDD141 pKa = 5.04LNEE144 pKa = 4.0ITVNFEE150 pKa = 3.63DD151 pKa = 5.48AKK153 pKa = 11.56VKK155 pKa = 10.52DD156 pKa = 3.86CPKK159 pKa = 10.18WADD162 pKa = 3.69EE163 pKa = 4.12PDD165 pKa = 3.19KK166 pKa = 11.28KK167 pKa = 10.51IIKK170 pKa = 8.65FTGKK174 pKa = 9.7FRR176 pKa = 11.84LPTLPTCPDD185 pKa = 3.16EE186 pKa = 4.61NGNGNGDD193 pKa = 3.59GNGNGNGNGNDD204 pKa = 3.53NGDD207 pKa = 3.71NGDD210 pKa = 4.25EE211 pKa = 4.33EE212 pKa = 5.38VCGCQVTGDD221 pKa = 4.43LNTGGNPDD229 pKa = 3.46EE230 pKa = 5.27RR231 pKa = 11.84VDD233 pKa = 5.87LNLQVCTGCTPEE245 pKa = 4.24NSLVSYY251 pKa = 11.39NNTLEE256 pKa = 5.13DD257 pKa = 4.07NDD259 pKa = 3.85IQLDD263 pKa = 4.11LSPDD267 pKa = 3.53DD268 pKa = 4.93EE269 pKa = 4.51EE270 pKa = 5.95PEE272 pKa = 5.15GIDD275 pKa = 4.74LVTCEE280 pKa = 4.86EE281 pKa = 3.87IAEE284 pKa = 4.25IEE286 pKa = 4.28GGLRR290 pKa = 11.84ATIEE294 pKa = 4.23GQASVTVGEE303 pKa = 4.94DD304 pKa = 2.83SFAACDD310 pKa = 3.71FTIVVTDD317 pKa = 4.1TPPPTSDD324 pKa = 3.05TIEE327 pKa = 4.07EE328 pKa = 4.17VEE330 pKa = 5.18IICDD334 pKa = 3.73DD335 pKa = 3.84QSVHH339 pKa = 5.47TVTEE343 pKa = 4.82PIEE346 pKa = 4.13GAQAINIQDD355 pKa = 3.66ICPLPPQQQ363 pKa = 4.62

Molecular weight: 39.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D9QPI9|D9QPI9_ACEAZ Dihydroorotase OS=Acetohalobium arabaticum (strain ATCC 49924 / DSM 5501 / Z-7288) OX=574087 GN=pyrC PE=3 SV=1
MM1 pKa = 7.12YY2 pKa = 10.92ALIRR6 pKa = 11.84LIDD9 pKa = 4.14LSFTIYY15 pKa = 9.3TWILIARR22 pKa = 11.84VISSWVSPPMHH33 pKa = 6.64NSNVRR38 pKa = 11.84KK39 pKa = 9.36IMKK42 pKa = 9.94FIYY45 pKa = 10.05EE46 pKa = 3.9VTEE49 pKa = 3.93PVLAPIRR56 pKa = 11.84RR57 pKa = 11.84MLPTGNIGIDD67 pKa = 4.17LSPLIAFIAINIIHH81 pKa = 6.61NSLLRR86 pKa = 11.84ILRR89 pKa = 11.84RR90 pKa = 11.84LLLYY94 pKa = 10.65

Molecular weight: 10.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2276

0

2276

703237

30

1535

309.0

34.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.788 ± 0.052

0.854 ± 0.023

6.15 ± 0.048

9.025 ± 0.064

3.67 ± 0.033

6.976 ± 0.046

1.456 ± 0.02

8.313 ± 0.049

7.104 ± 0.057

10.106 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.249 ± 0.022

4.686 ± 0.036

3.297 ± 0.028

3.63 ± 0.033

4.002 ± 0.033

5.5 ± 0.036

5.008 ± 0.032

7.15 ± 0.045

0.717 ± 0.016

3.319 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski