Mycobacterium virus Kugel

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8IB42|G8IB42_9CAUD Uncharacterized protein OS=Mycobacterium virus Kugel OX=1089128 GN=4 PE=4 SV=1
MM1 pKa = 7.77EE2 pKa = 6.48DD3 pKa = 3.64EE4 pKa = 5.94DD5 pKa = 6.01LDD7 pKa = 4.42ADD9 pKa = 5.56RR10 pKa = 11.84NDD12 pKa = 3.8CVHH15 pKa = 6.43GVEE18 pKa = 5.36GPCPVCLPEE27 pKa = 4.69LFEE30 pKa = 5.26DD31 pKa = 4.72GPYY34 pKa = 10.5QFFPQRR40 pKa = 11.84YY41 pKa = 5.99ITGVEE46 pKa = 3.99DD47 pKa = 3.63PLLIEE52 pKa = 4.71EE53 pKa = 4.7GG54 pKa = 3.62

Molecular weight: 6.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8IBC8|G8IBC8_9CAUD Uncharacterized protein OS=Mycobacterium virus Kugel OX=1089128 GN=90 PE=4 SV=1
MM1 pKa = 6.8TRR3 pKa = 11.84NGIGVGNPSRR13 pKa = 11.84NSSTNPLTRR22 pKa = 11.84NRR24 pKa = 11.84RR25 pKa = 11.84MPHH28 pKa = 4.13TT29 pKa = 4.09

Molecular weight: 3.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

16554

22

826

174.3

19.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.016 ± 0.38

0.755 ± 0.128

6.554 ± 0.222

6.627 ± 0.328

3.117 ± 0.152

8.747 ± 0.508

2.09 ± 0.154

4.784 ± 0.206

4.041 ± 0.257

8.342 ± 0.305

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.096 ± 0.103

3.099 ± 0.167

5.636 ± 0.25

3.171 ± 0.158

6.282 ± 0.403

5.938 ± 0.339

6.403 ± 0.273

7.201 ± 0.265

2.096 ± 0.118

3.002 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski