Bacillus phage vB_BanS-Tsamsa

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 272 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5J9I1|U5J9I1_9CAUD Uncharacterized protein OS=Bacillus phage vB_BanS-Tsamsa OX=1308863 PE=4 SV=1
MM1 pKa = 7.45NYY3 pKa = 9.57KK4 pKa = 10.61DD5 pKa = 3.82KK6 pKa = 11.03FVGITIYY13 pKa = 10.37GFCNGYY19 pKa = 9.98FGRR22 pKa = 11.84DD23 pKa = 3.19SYY25 pKa = 12.09DD26 pKa = 3.17DD27 pKa = 3.46KK28 pKa = 11.55TIIASGDD35 pKa = 2.78NWIVGKK41 pKa = 7.36TQYY44 pKa = 11.15GYY46 pKa = 11.31VEE48 pKa = 4.41FASFDD53 pKa = 3.57DD54 pKa = 4.72GEE56 pKa = 4.53TMEE59 pKa = 4.57EE60 pKa = 5.2LIGEE64 pKa = 4.4WSVEE68 pKa = 3.83PDD70 pKa = 2.89EE71 pKa = 5.98DD72 pKa = 3.96FF73 pKa = 5.02

Molecular weight: 8.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5JA80|U5JA80_9CAUD Uncharacterized protein OS=Bacillus phage vB_BanS-Tsamsa OX=1308863 PE=4 SV=1
MM1 pKa = 7.1KK2 pKa = 10.25RR3 pKa = 11.84KK4 pKa = 9.67RR5 pKa = 11.84VDD7 pKa = 3.27LYY9 pKa = 11.63SVLTRR14 pKa = 11.84NGYY17 pKa = 10.25KK18 pKa = 8.98MPRR21 pKa = 11.84YY22 pKa = 9.49SDD24 pKa = 3.19IVGVRR29 pKa = 11.84GDD31 pKa = 3.02IFRR34 pKa = 11.84FLNEE38 pKa = 3.97QVGLSNDD45 pKa = 3.34LTTCLATYY53 pKa = 10.74SDD55 pKa = 4.11SQWIKK60 pKa = 9.86LASRR64 pKa = 11.84LRR66 pKa = 11.84KK67 pKa = 8.73HH68 pKa = 4.63GHH70 pKa = 3.91VRR72 pKa = 11.84EE73 pKa = 4.46VVV75 pKa = 2.92

Molecular weight: 8.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

272

0

272

50136

28

3123

184.3

21.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.475 ± 0.308

0.882 ± 0.086

6.54 ± 0.132

8.154 ± 0.221

4.324 ± 0.127

6.401 ± 0.209

1.883 ± 0.122

6.967 ± 0.174

9.017 ± 0.176

7.954 ± 0.172

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.259 ± 0.136

5.579 ± 0.135

2.248 ± 0.102

3.395 ± 0.225

4.111 ± 0.149

5.431 ± 0.227

6.006 ± 0.233

6.706 ± 0.154

1.185 ± 0.068

4.484 ± 0.186
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski