Amino acid dipepetide frequency for Methanobrevibacter arboriphilus JCM 13429 = DSM 1125

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.236AlaAla: 3.236 ± 0.103
0.608AlaCys: 0.608 ± 0.036
2.899AlaAsp: 2.899 ± 0.072
3.132AlaGlu: 3.132 ± 0.082
2.158AlaPhe: 2.158 ± 0.066
3.74AlaGly: 3.74 ± 0.09
0.741AlaHis: 0.741 ± 0.036
6.354AlaIle: 6.354 ± 0.137
4.343AlaLys: 4.343 ± 0.093
4.511AlaLeu: 4.511 ± 0.113
1.35AlaMet: 1.35 ± 0.053
3.436AlaAsn: 3.436 ± 0.098
1.478AlaPro: 1.478 ± 0.056
1.078AlaGln: 1.078 ± 0.049
1.557AlaArg: 1.557 ± 0.07
3.387AlaSer: 3.387 ± 0.086
3.164AlaThr: 3.164 ± 0.096
3.436AlaVal: 3.436 ± 0.085
0.268AlaTrp: 0.268 ± 0.021
1.748AlaTyr: 1.748 ± 0.06
0.0AlaXaa: 0.0 ± 0.0
Cys
0.517CysAla: 0.517 ± 0.03
0.146CysCys: 0.146 ± 0.018
0.67CysAsp: 0.67 ± 0.038
0.733CysGlu: 0.733 ± 0.038
0.391CysPhe: 0.391 ± 0.028
1.092CysGly: 1.092 ± 0.062
0.186CysHis: 0.186 ± 0.018
0.823CysIle: 0.823 ± 0.043
0.692CysLys: 0.692 ± 0.035
0.687CysLeu: 0.687 ± 0.036
0.215CysMet: 0.215 ± 0.019
0.631CysAsn: 0.631 ± 0.039
0.687CysPro: 0.687 ± 0.053
0.22CysGln: 0.22 ± 0.016
0.296CysArg: 0.296 ± 0.024
0.67CysSer: 0.67 ± 0.036
0.506CysThr: 0.506 ± 0.03
0.667CysVal: 0.667 ± 0.038
0.066CysTrp: 0.066 ± 0.01
0.33CysTyr: 0.33 ± 0.027
0.0CysXaa: 0.0 ± 0.0
Asp
2.7AspAla: 2.7 ± 0.078
0.583AspCys: 0.583 ± 0.038
3.573AspAsp: 3.573 ± 0.096
4.699AspGlu: 4.699 ± 0.108
3.144AspPhe: 3.144 ± 0.083
3.702AspGly: 3.702 ± 0.121
0.738AspHis: 0.738 ± 0.036
6.564AspIle: 6.564 ± 0.13
4.696AspLys: 4.696 ± 0.104
5.545AspLeu: 5.545 ± 0.092
1.432AspMet: 1.432 ± 0.054
4.64AspAsn: 4.64 ± 0.118
1.932AspPro: 1.932 ± 0.072
0.872AspGln: 0.872 ± 0.043
1.464AspArg: 1.464 ± 0.052
4.056AspSer: 4.056 ± 0.093
2.41AspThr: 2.41 ± 0.071
3.936AspVal: 3.936 ± 0.105
0.399AspTrp: 0.399 ± 0.027
2.608AspTyr: 2.608 ± 0.072
0.0AspXaa: 0.0 ± 0.0
Glu
3.362GluAla: 3.362 ± 0.089
0.557GluCys: 0.557 ± 0.038
4.397GluAsp: 4.397 ± 0.1
5.549GluGlu: 5.549 ± 0.138
2.814GluPhe: 2.814 ± 0.07
3.683GluGly: 3.683 ± 0.084
0.862GluHis: 0.862 ± 0.039
7.287GluIle: 7.287 ± 0.148
6.989GluLys: 6.989 ± 0.161
5.913GluLeu: 5.913 ± 0.123
1.602GluMet: 1.602 ± 0.061
5.958GluAsn: 5.958 ± 0.113
1.37GluPro: 1.37 ± 0.054
1.11GluGln: 1.11 ± 0.051
1.871GluArg: 1.871 ± 0.068
3.849GluSer: 3.849 ± 0.097
3.108GluThr: 3.108 ± 0.081
3.898GluVal: 3.898 ± 0.099
0.422GluTrp: 0.422 ± 0.031
2.645GluTyr: 2.645 ± 0.065
0.0GluXaa: 0.0 ± 0.0
Phe
2.188PheAla: 2.188 ± 0.067
0.478PheCys: 0.478 ± 0.031
2.879PheAsp: 2.879 ± 0.064
2.855PheGlu: 2.855 ± 0.071
1.988PhePhe: 1.988 ± 0.069
2.889PheGly: 2.889 ± 0.084
0.621PheHis: 0.621 ± 0.034
4.575PheIle: 4.575 ± 0.117
3.85PheLys: 3.85 ± 0.083
4.041PheLeu: 4.041 ± 0.1
0.969PheMet: 0.969 ± 0.041
3.86PheAsn: 3.86 ± 0.128
1.301PhePro: 1.301 ± 0.052
0.88PheGln: 0.88 ± 0.042
1.216PheArg: 1.216 ± 0.046
3.265PheSer: 3.265 ± 0.086
2.433PheThr: 2.433 ± 0.096
2.571PheVal: 2.571 ± 0.066
0.269PheTrp: 0.269 ± 0.021
1.669PheTyr: 1.669 ± 0.051
0.002PheXaa: 0.002 ± 0.002
Gly
4.284GlyAla: 4.284 ± 0.119
0.821GlyCys: 0.821 ± 0.046
3.737GlyAsp: 3.737 ± 0.089
3.862GlyGlu: 3.862 ± 0.093
3.208GlyPhe: 3.208 ± 0.084
4.849GlyGly: 4.849 ± 0.151
0.969GlyHis: 0.969 ± 0.042
6.858GlyIle: 6.858 ± 0.13
5.182GlyLys: 5.182 ± 0.1
5.15GlyLeu: 5.15 ± 0.105
1.482GlyMet: 1.482 ± 0.05
4.575GlyAsn: 4.575 ± 0.166
1.606GlyPro: 1.606 ± 0.063
1.347GlyGln: 1.347 ± 0.057
1.907GlyArg: 1.907 ± 0.057
4.787GlySer: 4.787 ± 0.124
3.806GlyThr: 3.806 ± 0.092
5.038GlyVal: 5.038 ± 0.124
0.598GlyTrp: 0.598 ± 0.034
2.549GlyTyr: 2.549 ± 0.065
0.0GlyXaa: 0.0 ± 0.0
His
0.71HisAla: 0.71 ± 0.032
0.215HisCys: 0.215 ± 0.018
0.838HisAsp: 0.838 ± 0.038
0.964HisGlu: 0.964 ± 0.042
0.652HisPhe: 0.652 ± 0.033
1.048HisGly: 1.048 ± 0.043
0.322HisHis: 0.322 ± 0.029
1.293HisIle: 1.293 ± 0.05
0.981HisLys: 0.981 ± 0.045
1.275HisLeu: 1.275 ± 0.05
0.31HisMet: 0.31 ± 0.02
0.93HisAsn: 0.93 ± 0.043
0.719HisPro: 0.719 ± 0.037
0.333HisGln: 0.333 ± 0.024
0.453HisArg: 0.453 ± 0.029
0.871HisSer: 0.871 ± 0.042
0.706HisThr: 0.706 ± 0.033
0.856HisVal: 0.856 ± 0.04
0.11HisTrp: 0.11 ± 0.014
0.575HisTyr: 0.575 ± 0.035
0.0HisXaa: 0.0 ± 0.0
Ile
6.281IleAla: 6.281 ± 0.124
0.961IleCys: 0.961 ± 0.042
6.559IleAsp: 6.559 ± 0.128
6.769IleGlu: 6.769 ± 0.138
4.754IlePhe: 4.754 ± 0.115
6.623IleGly: 6.623 ± 0.132
1.477IleHis: 1.477 ± 0.051
10.798IleIle: 10.798 ± 0.184
8.206IleLys: 8.206 ± 0.141
9.054IleLeu: 9.054 ± 0.165
2.011IleMet: 2.011 ± 0.059
8.149IleAsn: 8.149 ± 0.187
3.707IlePro: 3.707 ± 0.088
1.93IleGln: 1.93 ± 0.057
2.735IleArg: 2.735 ± 0.066
8.011IleSer: 8.011 ± 0.144
5.723IleThr: 5.723 ± 0.149
6.152IleVal: 6.152 ± 0.103
0.595IleTrp: 0.595 ± 0.035
3.543IleTyr: 3.543 ± 0.117
0.0IleXaa: 0.0 ± 0.0
Lys
3.939LysAla: 3.939 ± 0.097
0.784LysCys: 0.784 ± 0.046
5.301LysAsp: 5.301 ± 0.111
6.965LysGlu: 6.965 ± 0.156
3.193LysPhe: 3.193 ± 0.084
4.379LysGly: 4.379 ± 0.091
1.135LysHis: 1.135 ± 0.047
9.103LysIle: 9.103 ± 0.145
8.381LysLys: 8.381 ± 0.158
6.768LysLeu: 6.768 ± 0.112
1.912LysMet: 1.912 ± 0.061
7.564LysAsn: 7.564 ± 0.138
2.096LysPro: 2.096 ± 0.059
1.657LysGln: 1.657 ± 0.056
2.604LysArg: 2.604 ± 0.07
5.585LysSer: 5.585 ± 0.106
4.488LysThr: 4.488 ± 0.1
4.627LysVal: 4.627 ± 0.102
0.562LysTrp: 0.562 ± 0.033
3.592LysTyr: 3.592 ± 0.094
0.0LysXaa: 0.0 ± 0.0
Leu
5.284LeuAla: 5.284 ± 0.119
0.729LeuCys: 0.729 ± 0.044
5.439LeuAsp: 5.439 ± 0.105
5.831LeuGlu: 5.831 ± 0.124
3.683LeuPhe: 3.683 ± 0.113
5.424LeuGly: 5.424 ± 0.107
1.004LeuHis: 1.004 ± 0.045
8.558LeuIle: 8.558 ± 0.167
7.884LeuLys: 7.884 ± 0.126
6.676LeuLeu: 6.676 ± 0.15
1.976LeuMet: 1.976 ± 0.066
6.726LeuAsn: 6.726 ± 0.124
2.623LeuPro: 2.623 ± 0.088
1.39LeuGln: 1.39 ± 0.045
2.461LeuArg: 2.461 ± 0.072
6.214LeuSer: 6.214 ± 0.1
4.284LeuThr: 4.284 ± 0.09
4.893LeuVal: 4.893 ± 0.095
0.453LeuTrp: 0.453 ± 0.031
2.462LeuTyr: 2.462 ± 0.08
0.0LeuXaa: 0.0 ± 0.0
Met
1.345MetAla: 1.345 ± 0.056
0.261MetCys: 0.261 ± 0.021
1.493MetAsp: 1.493 ± 0.062
1.606MetGlu: 1.606 ± 0.056
0.854MetPhe: 0.854 ± 0.033
1.634MetGly: 1.634 ± 0.059
0.329MetHis: 0.329 ± 0.026
2.129MetIle: 2.129 ± 0.068
2.127MetLys: 2.127 ± 0.057
1.565MetLeu: 1.565 ± 0.061
0.493MetMet: 0.493 ± 0.029
1.439MetAsn: 1.439 ± 0.049
0.734MetPro: 0.734 ± 0.035
0.435MetGln: 0.435 ± 0.026
0.682MetArg: 0.682 ± 0.034
1.523MetSer: 1.523 ± 0.05
0.977MetThr: 0.977 ± 0.042
1.427MetVal: 1.427 ± 0.049
0.12MetTrp: 0.12 ± 0.014
0.527MetTyr: 0.527 ± 0.029
0.0MetXaa: 0.0 ± 0.0
Asn
2.981AsnAla: 2.981 ± 0.087
0.871AsnCys: 0.871 ± 0.061
4.171AsnAsp: 4.171 ± 0.102
4.902AsnGlu: 4.902 ± 0.104
3.441AsnPhe: 3.441 ± 0.101
4.865AsnGly: 4.865 ± 0.177
1.13AsnHis: 1.13 ± 0.045
9.415AsnIle: 9.415 ± 0.21
6.746AsnLys: 6.746 ± 0.149
6.176AsnLeu: 6.176 ± 0.12
1.414AsnMet: 1.414 ± 0.051
9.591AsnAsn: 9.591 ± 0.411
2.769AsnPro: 2.769 ± 0.076
1.761AsnGln: 1.761 ± 0.078
2.022AsnArg: 2.022 ± 0.068
6.447AsnSer: 6.447 ± 0.226
4.269AsnThr: 4.269 ± 0.155
4.593AsnVal: 4.593 ± 0.139
0.614AsnTrp: 0.614 ± 0.035
3.541AsnTyr: 3.541 ± 0.122
0.0AsnXaa: 0.0 ± 0.0
Pro
1.347ProAla: 1.347 ± 0.057
0.32ProCys: 0.32 ± 0.022
1.827ProAsp: 1.827 ± 0.063
2.474ProGlu: 2.474 ± 0.083
1.459ProPhe: 1.459 ± 0.06
2.029ProGly: 2.029 ± 0.065
0.545ProHis: 0.545 ± 0.026
3.16ProIle: 3.16 ± 0.078
2.362ProLys: 2.362 ± 0.07
2.523ProLeu: 2.523 ± 0.084
0.657ProMet: 0.657 ± 0.033
2.034ProAsn: 2.034 ± 0.078
0.854ProPro: 0.854 ± 0.046
0.718ProGln: 0.718 ± 0.04
0.866ProArg: 0.866 ± 0.043
2.153ProSer: 2.153 ± 0.066
1.662ProThr: 1.662 ± 0.056
2.209ProVal: 2.209 ± 0.066
0.184ProTrp: 0.184 ± 0.022
1.125ProTyr: 1.125 ± 0.04
0.0ProXaa: 0.0 ± 0.0
Gln
1.128GlnAla: 1.128 ± 0.049
0.205GlnCys: 0.205 ± 0.018
0.981GlnAsp: 0.981 ± 0.043
1.391GlnGlu: 1.391 ± 0.056
0.734GlnPhe: 0.734 ± 0.034
1.245GlnGly: 1.245 ± 0.053
0.286GlnHis: 0.286 ± 0.023
1.887GlnIle: 1.887 ± 0.059
1.848GlnLys: 1.848 ± 0.055
1.763GlnLeu: 1.763 ± 0.059
0.549GlnMet: 0.549 ± 0.036
1.372GlnAsn: 1.372 ± 0.048
0.475GlnPro: 0.475 ± 0.03
0.44GlnGln: 0.44 ± 0.034
0.688GlnArg: 0.688 ± 0.041
1.275GlnSer: 1.275 ± 0.039
1.158GlnThr: 1.158 ± 0.043
1.105GlnVal: 1.105 ± 0.043
0.163GlnTrp: 0.163 ± 0.017
0.793GlnTyr: 0.793 ± 0.038
0.0GlnXaa: 0.0 ± 0.0
Arg
1.603ArgAla: 1.603 ± 0.065
0.335ArgCys: 0.335 ± 0.026
1.579ArgAsp: 1.579 ± 0.054
2.175ArgGlu: 2.175 ± 0.071
1.26ArgPhe: 1.26 ± 0.045
2.034ArgGly: 2.034 ± 0.064
0.424ArgHis: 0.424 ± 0.029
2.694ArgIle: 2.694 ± 0.069
2.497ArgLys: 2.497 ± 0.074
2.27ArgLeu: 2.27 ± 0.069
0.772ArgMet: 0.772 ± 0.036
1.795ArgAsn: 1.795 ± 0.062
0.866ArgPro: 0.866 ± 0.044
0.606ArgGln: 0.606 ± 0.039
1.122ArgArg: 1.122 ± 0.054
1.712ArgSer: 1.712 ± 0.057
1.482ArgThr: 1.482 ± 0.057
1.735ArgVal: 1.735 ± 0.061
0.179ArgTrp: 0.179 ± 0.017
1.015ArgTyr: 1.015 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
3.279SerAla: 3.279 ± 0.073
0.634SerCys: 0.634 ± 0.036
3.893SerAsp: 3.893 ± 0.093
4.042SerGlu: 4.042 ± 0.084
3.771SerPhe: 3.771 ± 0.112
5.256SerGly: 5.256 ± 0.134
1.04SerHis: 1.04 ± 0.04
6.909SerIle: 6.909 ± 0.122
6.068SerLys: 6.068 ± 0.128
6.15SerLeu: 6.15 ± 0.121
1.427SerMet: 1.427 ± 0.046
6.03SerAsn: 6.03 ± 0.202
2.096SerPro: 2.096 ± 0.065
1.588SerGln: 1.588 ± 0.056
1.968SerArg: 1.968 ± 0.054
6.037SerSer: 6.037 ± 0.151
3.684SerThr: 3.684 ± 0.082
4.302SerVal: 4.302 ± 0.105
0.486SerTrp: 0.486 ± 0.03
2.855SerTyr: 2.855 ± 0.082
0.0SerXaa: 0.0 ± 0.0
Thr
2.852ThrAla: 2.852 ± 0.094
0.494ThrCys: 0.494 ± 0.035
2.766ThrAsp: 2.766 ± 0.069
2.628ThrGlu: 2.628 ± 0.079
2.37ThrPhe: 2.37 ± 0.076
4.538ThrGly: 4.538 ± 0.136
0.83ThrHis: 0.83 ± 0.035
5.787ThrIle: 5.787 ± 0.145
3.484ThrLys: 3.484 ± 0.08
4.547ThrLeu: 4.547 ± 0.097
1.0ThrMet: 1.0 ± 0.043
4.478ThrAsn: 4.478 ± 0.172
1.891ThrPro: 1.891 ± 0.071
1.055ThrGln: 1.055 ± 0.041
1.38ThrArg: 1.38 ± 0.051
3.786ThrSer: 3.786 ± 0.087
3.077ThrThr: 3.077 ± 0.077
3.615ThrVal: 3.615 ± 0.111
0.314ThrTrp: 0.314 ± 0.023
1.905ThrTyr: 1.905 ± 0.076
0.0ThrXaa: 0.0 ± 0.0
Val
3.55ValAla: 3.55 ± 0.092
0.687ValCys: 0.687 ± 0.036
4.082ValAsp: 4.082 ± 0.099
4.015ValGlu: 4.015 ± 0.089
2.81ValPhe: 2.81 ± 0.079
4.164ValGly: 4.164 ± 0.103
0.81ValHis: 0.81 ± 0.044
5.912ValIle: 5.912 ± 0.109
4.782ValLys: 4.782 ± 0.099
5.44ValLeu: 5.44 ± 0.09
1.163ValMet: 1.163 ± 0.048
4.552ValAsn: 4.552 ± 0.149
1.966ValPro: 1.966 ± 0.059
1.117ValGln: 1.117 ± 0.047
1.6ValArg: 1.6 ± 0.061
4.744ValSer: 4.744 ± 0.125
3.422ValThr: 3.422 ± 0.109
4.075ValVal: 4.075 ± 0.114
0.335ValTrp: 0.335 ± 0.023
2.382ValTyr: 2.382 ± 0.072
0.0ValXaa: 0.0 ± 0.0
Trp
0.333TrpAla: 0.333 ± 0.023
0.061TrpCys: 0.061 ± 0.009
0.412TrpAsp: 0.412 ± 0.028
0.419TrpGlu: 0.419 ± 0.026
0.278TrpPhe: 0.278 ± 0.021
0.583TrpGly: 0.583 ± 0.03
0.103TrpHis: 0.103 ± 0.014
0.609TrpIle: 0.609 ± 0.036
0.516TrpLys: 0.516 ± 0.03
0.517TrpLeu: 0.517 ± 0.035
0.166TrpMet: 0.166 ± 0.018
0.56TrpAsn: 0.56 ± 0.03
0.143TrpPro: 0.143 ± 0.016
0.138TrpGln: 0.138 ± 0.015
0.21TrpArg: 0.21 ± 0.021
0.437TrpSer: 0.437 ± 0.034
0.31TrpThr: 0.31 ± 0.023
0.398TrpVal: 0.398 ± 0.028
0.145TrpTrp: 0.145 ± 0.016
0.278TrpTyr: 0.278 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.703TyrAla: 1.703 ± 0.05
0.473TyrCys: 0.473 ± 0.033
2.199TyrAsp: 2.199 ± 0.063
2.234TyrGlu: 2.234 ± 0.073
1.836TyrPhe: 1.836 ± 0.066
2.835TyrGly: 2.835 ± 0.092
0.611TyrHis: 0.611 ± 0.033
3.323TyrIle: 3.323 ± 0.089
3.001TyrLys: 3.001 ± 0.088
3.425TyrLeu: 3.425 ± 0.082
0.8TyrMet: 0.8 ± 0.038
3.402TyrAsn: 3.402 ± 0.138
1.194TyrPro: 1.194 ± 0.045
0.81TyrGln: 0.81 ± 0.04
1.053TyrArg: 1.053 ± 0.044
2.707TyrSer: 2.707 ± 0.082
2.129TyrThr: 2.129 ± 0.081
2.073TyrVal: 2.073 ± 0.069
0.335TyrTrp: 0.335 ± 0.022
1.728TyrTyr: 1.728 ± 0.063
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.002XaaSer: 0.002 ± 0.002
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1961 proteins (608793 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski