Tobacco leaf curl Japan betasatellite
Average proteome isoelectric point is 3.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A4F1V8|A4F1V8_9VIRU C1 protein OS=Tobacco leaf curl Japan betasatellite OX=2010326 GN=betaC1 PE=4 SV=1
MM1 pKa = 7.71 TITYY5 pKa = 10.85 SNGNGIKK12 pKa = 10.32 FIIDD16 pKa = 3.05 VRR18 pKa = 11.84 LQQFISVSVQVYY30 pKa = 7.32 STNQAILTGFNCNIPYY46 pKa = 9.53 TYY48 pKa = 9.96 EE49 pKa = 3.8 QIIAPFDD56 pKa = 3.99 FNGTEE61 pKa = 3.94 EE62 pKa = 4.48 IIRR65 pKa = 11.84 NTLEE69 pKa = 4.58 LMYY72 pKa = 10.59 EE73 pKa = 4.31 DD74 pKa = 4.8 SDD76 pKa = 3.82 VSNFQVEE83 pKa = 4.49 DD84 pKa = 3.77 MIEE87 pKa = 4.28 TIDD90 pKa = 3.76 ILMTEE95 pKa = 4.92 MFTDD99 pKa = 3.6 MGVEE103 pKa = 4.1 TMRR106 pKa = 11.84 RR107 pKa = 11.84 CIVRR111 pKa = 11.84 HH112 pKa = 5.93 KK113 pKa = 9.99 YY114 pKa = 7.98 TVV116 pKa = 2.82
Molecular weight: 13.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.976
IPC2_protein 4.291
IPC_protein 4.177
Toseland 4.012
ProMoST 4.304
Dawson 4.139
Bjellqvist 4.291
Wikipedia 4.024
Rodwell 4.012
Grimsley 3.923
Solomon 4.126
Lehninger 4.075
Nozaki 4.253
DTASelect 4.406
Thurlkill 4.037
EMBOSS 4.037
Sillero 4.291
Patrickios 1.964
IPC_peptide 4.126
IPC2_peptide 4.279
IPC2.peptide.svr19 4.186
Protein with the highest isoelectric point:
>tr|A4F1V8|A4F1V8_9VIRU C1 protein OS=Tobacco leaf curl Japan betasatellite OX=2010326 GN=betaC1 PE=4 SV=1
MM1 pKa = 7.71 TITYY5 pKa = 10.85 SNGNGIKK12 pKa = 10.32 FIIDD16 pKa = 3.05 VRR18 pKa = 11.84 LQQFISVSVQVYY30 pKa = 7.32 STNQAILTGFNCNIPYY46 pKa = 9.53 TYY48 pKa = 9.96 EE49 pKa = 3.8 QIIAPFDD56 pKa = 3.99 FNGTEE61 pKa = 3.94 EE62 pKa = 4.48 IIRR65 pKa = 11.84 NTLEE69 pKa = 4.58 LMYY72 pKa = 10.59 EE73 pKa = 4.31 DD74 pKa = 4.8 SDD76 pKa = 3.82 VSNFQVEE83 pKa = 4.49 DD84 pKa = 3.77 MIEE87 pKa = 4.28 TIDD90 pKa = 3.76 ILMTEE95 pKa = 4.92 MFTDD99 pKa = 3.6 MGVEE103 pKa = 4.1 TMRR106 pKa = 11.84 RR107 pKa = 11.84 CIVRR111 pKa = 11.84 HH112 pKa = 5.93 KK113 pKa = 9.99 YY114 pKa = 7.98 TVV116 pKa = 2.82
Molecular weight: 13.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.976
IPC2_protein 4.291
IPC_protein 4.177
Toseland 4.012
ProMoST 4.304
Dawson 4.139
Bjellqvist 4.291
Wikipedia 4.024
Rodwell 4.012
Grimsley 3.923
Solomon 4.126
Lehninger 4.075
Nozaki 4.253
DTASelect 4.406
Thurlkill 4.037
EMBOSS 4.037
Sillero 4.291
Patrickios 1.964
IPC_peptide 4.126
IPC2_peptide 4.279
IPC2.peptide.svr19 4.186
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
116
116
116
116.0
13.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
1.724 ± 0.0
1.724 ± 0.0
6.034 ± 0.0
7.759 ± 0.0
6.034 ± 0.0
4.31 ± 0.0
0.862 ± 0.0
12.931 ± 0.0
1.724 ± 0.0
4.31 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
6.034 ± 0.0
6.897 ± 0.0
1.724 ± 0.0
5.172 ± 0.0
4.31 ± 0.0
5.172 ± 0.0
10.345 ± 0.0
7.759 ± 0.0
0.0 ± 0.0
5.172 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here