Lynx canadensis faeces associated genomovirus CL1 58
Average proteome isoelectric point is 7.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5CI18|A0A2Z5CI18_9VIRU Replication-associated protein OS=Lynx canadensis faeces associated genomovirus CL1 58 OX=2219126 PE=3 SV=1
MM1 pKa = 7.75 PSFVANFKK9 pKa = 10.24 YY10 pKa = 10.83 ALVTYY15 pKa = 6.91 SQCGDD20 pKa = 3.79 LDD22 pKa = 3.55 PWSVMEE28 pKa = 4.86 LFSSLGAEE36 pKa = 4.24 CIIGRR41 pKa = 11.84 EE42 pKa = 4.01 HH43 pKa = 7.75 HH44 pKa = 6.83 EE45 pKa = 4.72 DD46 pKa = 3.59 GGTHH50 pKa = 5.89 LHH52 pKa = 5.88 VFVDD56 pKa = 4.55 FGRR59 pKa = 11.84 KK60 pKa = 7.93 FRR62 pKa = 11.84 SRR64 pKa = 11.84 ATNVFDD70 pKa = 5.08 VDD72 pKa = 3.5 GHH74 pKa = 6.44 HH75 pKa = 7.29 PNIEE79 pKa = 4.06 PSKK82 pKa = 8.78 GTPEE86 pKa = 4.01 KK87 pKa = 11.01 GFDD90 pKa = 3.46 YY91 pKa = 10.65 AIKK94 pKa = 10.85 DD95 pKa = 3.45 GDD97 pKa = 4.33 VVCGGLGRR105 pKa = 11.84 PEE107 pKa = 4.06 PSRR110 pKa = 11.84 GGNSGVLAKK119 pKa = 7.87 WTRR122 pKa = 11.84 ITGATDD128 pKa = 3.13 KK129 pKa = 11.52 QEE131 pKa = 4.11 FWDD134 pKa = 4.65 LVHH137 pKa = 6.98 EE138 pKa = 5.1 LDD140 pKa = 4.6 PKK142 pKa = 10.76 SAACSFTSLSKK153 pKa = 10.06 YY154 pKa = 10.56 ADD156 pKa = 2.96 WKK158 pKa = 10.68 FAVDD162 pKa = 4.22 PPCYY166 pKa = 8.96 EE167 pKa = 4.02 HH168 pKa = 7.56 PGGFDD173 pKa = 3.41 FSDD176 pKa = 3.9 GSFDD180 pKa = 4.32 GRR182 pKa = 11.84 SEE184 pKa = 4.2 CKK186 pKa = 10.23 SLVMYY191 pKa = 10.37 GPSRR195 pKa = 11.84 TGKK198 pKa = 8.21 TMWARR203 pKa = 11.84 SLGAHH208 pKa = 7.19 LYY210 pKa = 10.52 CVGLLSGDD218 pKa = 3.43 EE219 pKa = 4.17 CMKK222 pKa = 10.97 AEE224 pKa = 4.07 YY225 pKa = 10.43 ADD227 pKa = 3.75 YY228 pKa = 11.08 AIFDD232 pKa = 4.25 DD233 pKa = 4.69 LRR235 pKa = 11.84 GGVKK239 pKa = 9.8 FFPAFKK245 pKa = 9.49 EE246 pKa = 3.89 WLGAQQYY253 pKa = 7.12 VTVKK257 pKa = 10.5 RR258 pKa = 11.84 LYY260 pKa = 10.56 HH261 pKa = 5.72 EE262 pKa = 4.69 PKK264 pKa = 9.56 LVKK267 pKa = 9.05 WGKK270 pKa = 9.69 PSIFLTNSDD279 pKa = 4.19 PRR281 pKa = 11.84 DD282 pKa = 3.48 EE283 pKa = 4.34 ATGDD287 pKa = 3.5 DD288 pKa = 4.13 RR289 pKa = 11.84 DD290 pKa = 3.78 WLEE293 pKa = 4.08 RR294 pKa = 11.84 NCDD297 pKa = 4.42 FIYY300 pKa = 9.55 VQEE303 pKa = 5.02 PIFHH307 pKa = 7.21 ANTEE311 pKa = 4.14
Molecular weight: 34.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.175
IPC2_protein 5.207
IPC_protein 5.194
Toseland 5.308
ProMoST 5.334
Dawson 5.296
Bjellqvist 5.397
Wikipedia 5.232
Rodwell 5.219
Grimsley 5.308
Solomon 5.296
Lehninger 5.258
Nozaki 5.461
DTASelect 5.664
Thurlkill 5.397
EMBOSS 5.385
Sillero 5.537
Patrickios 3.834
IPC_peptide 5.296
IPC2_peptide 5.537
IPC2.peptide.svr19 5.469
Protein with the highest isoelectric point:
>tr|A0A2Z5CI18|A0A2Z5CI18_9VIRU Replication-associated protein OS=Lynx canadensis faeces associated genomovirus CL1 58 OX=2219126 PE=3 SV=1
MM1 pKa = 7.8 PARR4 pKa = 11.84 AYY6 pKa = 9.86 RR7 pKa = 11.84 RR8 pKa = 11.84 KK9 pKa = 9.34 SGSYY13 pKa = 9.45 RR14 pKa = 11.84 GRR16 pKa = 11.84 KK17 pKa = 9.06 RR18 pKa = 11.84 GGPTKK23 pKa = 10.37 RR24 pKa = 11.84 AWSNYY29 pKa = 8.11 KK30 pKa = 10.19 RR31 pKa = 11.84 STGRR35 pKa = 11.84 TRR37 pKa = 11.84 PYY39 pKa = 8.19 RR40 pKa = 11.84 TSRR43 pKa = 11.84 TRR45 pKa = 11.84 QKK47 pKa = 10.64 PMSRR51 pKa = 11.84 KK52 pKa = 9.74 KK53 pKa = 10.01 ILNVTSEE60 pKa = 4.03 KK61 pKa = 10.67 KK62 pKa = 9.49 RR63 pKa = 11.84 DD64 pKa = 4.56 KK65 pKa = 10.14 MLCWTNTIAGTPQGGTTYY83 pKa = 8.82 TQSPAILTGAGADD96 pKa = 3.47 PYY98 pKa = 11.28 LFAWCATARR107 pKa = 11.84 DD108 pKa = 4.19 NTTSTGGAAHH118 pKa = 7.57 AGTKK122 pKa = 9.38 FDD124 pKa = 3.59 KK125 pKa = 10.21 ATRR128 pKa = 11.84 TASEE132 pKa = 4.82 CYY134 pKa = 8.93 MVGLSEE140 pKa = 5.4 RR141 pKa = 11.84 IEE143 pKa = 4.04 IQCADD148 pKa = 3.69 GLPWQWRR155 pKa = 11.84 RR156 pKa = 11.84 ICFTMKK162 pKa = 10.51 GGVGLDD168 pKa = 3.5 GTLTTGSTFSSYY180 pKa = 11.46 SEE182 pKa = 4.15 TTSGYY187 pKa = 9.17 VRR189 pKa = 11.84 TVNSVLLTDD198 pKa = 3.86 RR199 pKa = 11.84 TSLYY203 pKa = 11.13 SLLFAGAQSSDD214 pKa = 2.6 WSDD217 pKa = 3.44 PMVAPLDD224 pKa = 3.53 RR225 pKa = 11.84 RR226 pKa = 11.84 RR227 pKa = 11.84 INVKK231 pKa = 9.65 YY232 pKa = 10.89 DD233 pKa = 2.98 KK234 pKa = 8.37 TTSIASGNEE243 pKa = 3.47 DD244 pKa = 3.28 GVIRR248 pKa = 11.84 KK249 pKa = 5.55 YY250 pKa = 9.38 TKK252 pKa = 7.92 WHH254 pKa = 6.36 PMGKK258 pKa = 7.54 TLMYY262 pKa = 10.87 DD263 pKa = 3.38 DD264 pKa = 5.72 DD265 pKa = 5.0 EE266 pKa = 4.65 NTGAEE271 pKa = 4.18 NPAVISVDD279 pKa = 3.2 NKK281 pKa = 10.65 QGMGDD286 pKa = 3.57 FWVLDD291 pKa = 4.45 FIKK294 pKa = 10.35 PRR296 pKa = 11.84 MGSTSSNQLSFRR308 pKa = 11.84 PEE310 pKa = 3.66 STLYY314 pKa = 8.15 WHH316 pKa = 6.78 EE317 pKa = 4.04 RR318 pKa = 3.41
Molecular weight: 35.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.232
IPC2_protein 9.37
IPC_protein 9.487
Toseland 9.97
ProMoST 9.765
Dawson 10.204
Bjellqvist 9.911
Wikipedia 10.394
Rodwell 10.526
Grimsley 10.292
Solomon 10.233
Lehninger 10.189
Nozaki 10.014
DTASelect 9.897
Thurlkill 10.058
EMBOSS 10.394
Sillero 10.131
Patrickios 9.823
IPC_peptide 10.233
IPC2_peptide 8.712
IPC2.peptide.svr19 8.325
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
629
311
318
314.5
35.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.677 ± 0.172
2.226 ± 0.466
7.154 ± 1.066
4.61 ± 1.046
4.61 ± 1.495
9.539 ± 0.299
2.226 ± 0.915
3.18 ± 0.199
6.2 ± 0.064
6.2 ± 0.385
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.385 ± 0.542
2.862 ± 0.426
4.928 ± 0.375
2.067 ± 0.32
6.995 ± 1.516
8.267 ± 0.833
8.108 ± 2.517
4.928 ± 0.824
2.703 ± 0.091
4.134 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here