Ecklonia radiata-associated virus 5

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 7.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G2CHT1|A0A3G2CHT1_9CIRC Rep-A OS=Ecklonia radiata-associated virus 5 OX=2480195 PE=4 SV=1
MM1 pKa = 7.45SVPSSTRR8 pKa = 11.84GKK10 pKa = 9.93RR11 pKa = 11.84WVFTLNNHH19 pKa = 5.76TEE21 pKa = 4.25GEE23 pKa = 4.36VQLLSDD29 pKa = 5.04LLTSEE34 pKa = 4.28HH35 pKa = 5.71VSYY38 pKa = 10.99GIFGRR43 pKa = 11.84EE44 pKa = 3.95VGEE47 pKa = 4.05SGTPHH52 pKa = 6.02LQGYY56 pKa = 9.26CIFADD61 pKa = 3.73RR62 pKa = 11.84KK63 pKa = 10.34RR64 pKa = 11.84FNQVRR69 pKa = 11.84ALFGXRR75 pKa = 11.84YY76 pKa = 8.88HH77 pKa = 8.42VEE79 pKa = 3.61ISRR82 pKa = 11.84GTPAQASQYY91 pKa = 9.62CKK93 pKa = 10.25KK94 pKa = 10.78DD95 pKa = 2.95GDD97 pKa = 3.85FEE99 pKa = 4.65EE100 pKa = 5.61FGEE103 pKa = 4.89FPGTQGKK110 pKa = 10.12RR111 pKa = 11.84SDD113 pKa = 3.65WEE115 pKa = 4.42SLKK118 pKa = 10.68LWCEE122 pKa = 3.93TQTRR126 pKa = 11.84QPTDD130 pKa = 3.17LEE132 pKa = 4.16LFYY135 pKa = 11.03AFPSLFGRR143 pKa = 11.84YY144 pKa = 7.03EE145 pKa = 3.78KK146 pKa = 10.51SVRR149 pKa = 11.84KK150 pKa = 9.6ICNLCIKK157 pKa = 10.07CDD159 pKa = 3.6PXXIGEE165 pKa = 3.93PRR167 pKa = 11.84GWQRR171 pKa = 11.84DD172 pKa = 3.63XEE174 pKa = 4.31EE175 pKa = 4.99RR176 pKa = 11.84LLDD179 pKa = 4.29EE180 pKa = 5.42PDD182 pKa = 3.54DD183 pKa = 4.04RR184 pKa = 11.84CIEE187 pKa = 3.96FVLDD191 pKa = 3.54YY192 pKa = 10.34EE193 pKa = 4.72GNSGKK198 pKa = 10.09SWFVRR203 pKa = 11.84YY204 pKa = 10.29YY205 pKa = 9.02MLKK208 pKa = 9.54HH209 pKa = 5.8QGSAQMLSVGKK220 pKa = 10.1RR221 pKa = 11.84DD222 pKa = 5.39DD223 pKa = 3.38IAHH226 pKa = 6.4CXDD229 pKa = 3.01VSRR232 pKa = 11.84RR233 pKa = 11.84VFFFDD238 pKa = 3.18IPRR241 pKa = 11.84GGMEE245 pKa = 4.14FLQYY249 pKa = 10.89GALEE253 pKa = 4.72GIKK256 pKa = 10.44NGFIFSPKK264 pKa = 9.74YY265 pKa = 10.1EE266 pKa = 4.26STTKK270 pKa = 10.58VLLNKK275 pKa = 9.86AHH277 pKa = 6.52VVVLCNEE284 pKa = 4.4DD285 pKa = 3.69PDD287 pKa = 4.11MLKK290 pKa = 10.88LSLDD294 pKa = 3.46RR295 pKa = 11.84YY296 pKa = 10.62KK297 pKa = 9.77ITNLXXLL304 pKa = 5.31

Molecular weight: 34.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G2CHT1|A0A3G2CHT1_9CIRC Rep-A OS=Ecklonia radiata-associated virus 5 OX=2480195 PE=4 SV=1
MM1 pKa = 7.93AIRR4 pKa = 11.84RR5 pKa = 11.84KK6 pKa = 10.17RR7 pKa = 11.84IILPLHH13 pKa = 6.16RR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84VRR18 pKa = 11.84PRR20 pKa = 11.84LYY22 pKa = 8.93KK23 pKa = 10.28TKK25 pKa = 9.06TRR27 pKa = 11.84TYY29 pKa = 9.95RR30 pKa = 11.84RR31 pKa = 11.84SYY33 pKa = 9.04GNKK36 pKa = 9.05RR37 pKa = 11.84MRR39 pKa = 11.84RR40 pKa = 11.84MQDD43 pKa = 3.05FSPRR47 pKa = 11.84AIANPVGLSNAKK59 pKa = 8.57TRR61 pKa = 11.84LLTFSQDD68 pKa = 3.18SPDD71 pKa = 3.58RR72 pKa = 11.84AFVPYY77 pKa = 10.4IMTNIPFTAANAPDD91 pKa = 3.39SRR93 pKa = 11.84QRR95 pKa = 11.84NIINIRR101 pKa = 11.84GFKK104 pKa = 9.35IQVVFQNTQRR114 pKa = 11.84PAFYY118 pKa = 10.68LNWAVIHH125 pKa = 6.51PKK127 pKa = 10.69LSIDD131 pKa = 3.65DD132 pKa = 4.28TSVSTQDD139 pKa = 3.7FFRR142 pKa = 11.84TYY144 pKa = 9.82NANRR148 pKa = 11.84AVDD151 pKa = 5.6LITDD155 pKa = 3.46NTGVVNNIAQDD166 pKa = 3.79VPTHH170 pKa = 5.77GMSSINTDD178 pKa = 2.21KK179 pKa = 10.91WVVLKK184 pKa = 10.54HH185 pKa = 6.04KK186 pKa = 9.37RR187 pKa = 11.84TLLAGAINNPDD198 pKa = 3.49DD199 pKa = 3.65NALXFXATNCQIPKK213 pKa = 9.65GNRR216 pKa = 11.84VVIDD220 pKa = 3.85KK221 pKa = 9.38YY222 pKa = 11.18IKK224 pKa = 9.45MGRR227 pKa = 11.84QFRR230 pKa = 11.84FPNDD234 pKa = 3.22DD235 pKa = 3.31STFPEE240 pKa = 4.67GGNTIFCYY248 pKa = 10.14WLTNLNNYY256 pKa = 9.69LGSQNTSXDD265 pKa = 3.31VSVIRR270 pKa = 11.84TTGRR274 pKa = 11.84IITYY278 pKa = 9.72FRR280 pKa = 11.84EE281 pKa = 4.34TKK283 pKa = 10.27NN284 pKa = 3.15

Molecular weight: 32.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

588

284

304

294.0

33.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.422 ± 1.0

1.871 ± 0.746

6.122 ± 0.087

4.252 ± 2.268

5.782 ± 0.32

6.293 ± 1.321

1.871 ± 0.296

5.952 ± 1.372

5.442 ± 0.328

7.483 ± 1.182

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.871 ± 0.155

6.122 ± 2.164

4.592 ± 0.441

3.912 ± 0.025

8.844 ± 1.099

6.122 ± 0.537

6.463 ± 1.496

5.782 ± 0.13

1.361 ± 0.194

3.741 ± 0.141
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski