Amino acid dipepetide frequency for Clostridium saccharolyticum (strain ATCC 35040 / DSM 2544 / NRCC 2533 / WM1)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.816AlaAla: 7.816 ± 0.104
1.123AlaCys: 1.123 ± 0.031
4.199AlaAsp: 4.199 ± 0.059
5.151AlaGlu: 5.151 ± 0.065
3.133AlaPhe: 3.133 ± 0.052
6.723AlaGly: 6.723 ± 0.093
1.041AlaHis: 1.041 ± 0.027
4.853AlaIle: 4.853 ± 0.075
4.327AlaLys: 4.327 ± 0.059
7.051AlaLeu: 7.051 ± 0.079
2.503AlaMet: 2.503 ± 0.043
2.472AlaAsn: 2.472 ± 0.046
2.152AlaPro: 2.152 ± 0.053
2.103AlaGln: 2.103 ± 0.051
3.09AlaArg: 3.09 ± 0.048
4.321AlaSer: 4.321 ± 0.065
3.036AlaThr: 3.036 ± 0.07
6.476AlaVal: 6.476 ± 0.072
0.677AlaTrp: 0.677 ± 0.025
2.819AlaTyr: 2.819 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
0.875CysAla: 0.875 ± 0.025
0.275CysCys: 0.275 ± 0.018
0.755CysAsp: 0.755 ± 0.024
0.808CysGlu: 0.808 ± 0.027
0.648CysPhe: 0.648 ± 0.023
1.397CysGly: 1.397 ± 0.036
0.288CysHis: 0.288 ± 0.015
0.982CysIle: 0.982 ± 0.028
0.708CysLys: 0.708 ± 0.028
1.21CysLeu: 1.21 ± 0.034
0.436CysMet: 0.436 ± 0.02
0.581CysAsn: 0.581 ± 0.022
0.633CysPro: 0.633 ± 0.042
0.403CysGln: 0.403 ± 0.017
0.79CysArg: 0.79 ± 0.03
0.936CysSer: 0.936 ± 0.029
0.691CysThr: 0.691 ± 0.025
0.815CysVal: 0.815 ± 0.022
0.121CysTrp: 0.121 ± 0.009
0.542CysTyr: 0.542 ± 0.022
0.0CysXaa: 0.0 ± 0.0
Asp
3.477AspAla: 3.477 ± 0.06
0.793AspCys: 0.793 ± 0.024
2.495AspAsp: 2.495 ± 0.051
3.973AspGlu: 3.973 ± 0.057
2.545AspPhe: 2.545 ± 0.042
4.418AspGly: 4.418 ± 0.071
1.122AspHis: 1.122 ± 0.035
4.149AspIle: 4.149 ± 0.064
3.257AspLys: 3.257 ± 0.053
4.705AspLeu: 4.705 ± 0.066
1.911AspMet: 1.911 ± 0.042
2.01AspAsn: 2.01 ± 0.037
1.943AspPro: 1.943 ± 0.045
1.693AspGln: 1.693 ± 0.039
2.619AspArg: 2.619 ± 0.05
3.112AspSer: 3.112 ± 0.051
2.771AspThr: 2.771 ± 0.051
3.3AspVal: 3.3 ± 0.056
0.603AspTrp: 0.603 ± 0.023
2.665AspTyr: 2.665 ± 0.053
0.0AspXaa: 0.0 ± 0.0
Glu
5.808GluAla: 5.808 ± 0.076
0.764GluCys: 0.764 ± 0.028
3.934GluAsp: 3.934 ± 0.055
7.06GluGlu: 7.06 ± 0.103
2.496GluPhe: 2.496 ± 0.055
4.664GluGly: 4.664 ± 0.07
1.276GluHis: 1.276 ± 0.03
5.457GluIle: 5.457 ± 0.069
5.931GluLys: 5.931 ± 0.076
6.527GluLeu: 6.527 ± 0.092
2.232GluMet: 2.232 ± 0.047
3.717GluAsn: 3.717 ± 0.057
1.977GluPro: 1.977 ± 0.041
2.628GluGln: 2.628 ± 0.051
3.396GluArg: 3.396 ± 0.057
3.583GluSer: 3.583 ± 0.056
3.891GluThr: 3.891 ± 0.062
4.379GluVal: 4.379 ± 0.064
0.632GluTrp: 0.632 ± 0.021
2.909GluTyr: 2.909 ± 0.055
0.0GluXaa: 0.0 ± 0.0
Phe
2.819PheAla: 2.819 ± 0.047
0.676PheCys: 0.676 ± 0.024
2.467PheAsp: 2.467 ± 0.043
2.599PheGlu: 2.599 ± 0.05
1.876PhePhe: 1.876 ± 0.04
3.092PheGly: 3.092 ± 0.046
0.909PheHis: 0.909 ± 0.029
2.902PheIle: 2.902 ± 0.048
2.152PheLys: 2.152 ± 0.039
4.334PheLeu: 4.334 ± 0.079
1.236PheMet: 1.236 ± 0.032
1.612PheAsn: 1.612 ± 0.036
1.487PhePro: 1.487 ± 0.031
1.503PheGln: 1.503 ± 0.037
1.698PheArg: 1.698 ± 0.039
3.004PheSer: 3.004 ± 0.049
2.414PheThr: 2.414 ± 0.051
2.642PheVal: 2.642 ± 0.049
0.404PheTrp: 0.404 ± 0.019
1.67PheTyr: 1.67 ± 0.037
0.0PheXaa: 0.0 ± 0.0
Gly
5.373GlyAla: 5.373 ± 0.09
1.198GlyCys: 1.198 ± 0.032
3.618GlyAsp: 3.618 ± 0.054
5.022GlyGlu: 5.022 ± 0.067
3.392GlyPhe: 3.392 ± 0.052
5.406GlyGly: 5.406 ± 0.091
1.247GlyHis: 1.247 ± 0.033
6.806GlyIle: 6.806 ± 0.094
5.545GlyLys: 5.545 ± 0.067
6.449GlyLeu: 6.449 ± 0.082
2.547GlyMet: 2.547 ± 0.047
3.355GlyAsn: 3.355 ± 0.064
1.823GlyPro: 1.823 ± 0.122
2.27GlyGln: 2.27 ± 0.046
3.359GlyArg: 3.359 ± 0.055
4.618GlySer: 4.618 ± 0.088
4.487GlyThr: 4.487 ± 0.075
5.031GlyVal: 5.031 ± 0.073
0.806GlyTrp: 0.806 ± 0.031
3.34GlyTyr: 3.34 ± 0.063
0.0GlyXaa: 0.0 ± 0.0
His
1.073HisAla: 1.073 ± 0.024
0.293HisCys: 0.293 ± 0.015
0.883HisAsp: 0.883 ± 0.031
1.049HisGlu: 1.049 ± 0.029
0.836HisPhe: 0.836 ± 0.023
1.4HisGly: 1.4 ± 0.039
0.425HisHis: 0.425 ± 0.021
1.337HisIle: 1.337 ± 0.031
1.011HisLys: 1.011 ± 0.028
1.66HisLeu: 1.66 ± 0.041
0.584HisMet: 0.584 ± 0.021
0.696HisAsn: 0.696 ± 0.024
0.766HisPro: 0.766 ± 0.025
0.531HisGln: 0.531 ± 0.023
0.746HisArg: 0.746 ± 0.023
1.002HisSer: 1.002 ± 0.032
0.916HisThr: 0.916 ± 0.025
1.13HisVal: 1.13 ± 0.025
0.173HisTrp: 0.173 ± 0.011
0.779HisTyr: 0.779 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
5.439IleAla: 5.439 ± 0.067
1.188IleCys: 1.188 ± 0.033
3.855IleAsp: 3.855 ± 0.065
4.34IleGlu: 4.34 ± 0.066
2.771IlePhe: 2.771 ± 0.049
5.293IleGly: 5.293 ± 0.075
1.436IleHis: 1.436 ± 0.04
5.116IleIle: 5.116 ± 0.088
4.266IleLys: 4.266 ± 0.063
7.2IleLeu: 7.2 ± 0.096
2.062IleMet: 2.062 ± 0.046
3.068IleAsn: 3.068 ± 0.053
3.343IlePro: 3.343 ± 0.055
2.426IleGln: 2.426 ± 0.048
3.828IleArg: 3.828 ± 0.057
5.15IleSer: 5.15 ± 0.072
4.41IleThr: 4.41 ± 0.064
4.391IleVal: 4.391 ± 0.06
0.663IleTrp: 0.663 ± 0.024
2.744IleTyr: 2.744 ± 0.045
0.0IleXaa: 0.0 ± 0.0
Lys
5.148LysAla: 5.148 ± 0.071
0.609LysCys: 0.609 ± 0.025
3.846LysAsp: 3.846 ± 0.06
6.465LysGlu: 6.465 ± 0.09
1.749LysPhe: 1.749 ± 0.037
4.487LysGly: 4.487 ± 0.056
0.907LysHis: 0.907 ± 0.026
4.439LysIle: 4.439 ± 0.057
5.395LysLys: 5.395 ± 0.077
5.227LysLeu: 5.227 ± 0.069
1.933LysMet: 1.933 ± 0.038
3.468LysAsn: 3.468 ± 0.055
2.073LysPro: 2.073 ± 0.042
2.262LysGln: 2.262 ± 0.047
3.091LysArg: 3.091 ± 0.047
3.448LysSer: 3.448 ± 0.053
3.761LysThr: 3.761 ± 0.06
3.963LysVal: 3.963 ± 0.056
0.591LysTrp: 0.591 ± 0.023
2.452LysTyr: 2.452 ± 0.047
0.0LysXaa: 0.0 ± 0.0
Leu
6.877LeuAla: 6.877 ± 0.072
1.423LeuCys: 1.423 ± 0.037
4.822LeuAsp: 4.822 ± 0.064
6.456LeuGlu: 6.456 ± 0.079
4.033LeuPhe: 4.033 ± 0.065
6.181LeuGly: 6.181 ± 0.069
1.469LeuHis: 1.469 ± 0.038
6.063LeuIle: 6.063 ± 0.087
6.614LeuLys: 6.614 ± 0.076
8.846LeuLeu: 8.846 ± 0.104
2.857LeuMet: 2.857 ± 0.048
3.959LeuAsn: 3.959 ± 0.054
3.797LeuPro: 3.797 ± 0.058
2.618LeuGln: 2.618 ± 0.04
3.615LeuArg: 3.615 ± 0.06
6.91LeuSer: 6.91 ± 0.082
5.273LeuThr: 5.273 ± 0.07
5.645LeuVal: 5.645 ± 0.074
0.826LeuTrp: 0.826 ± 0.029
3.301LeuTyr: 3.301 ± 0.051
0.0LeuXaa: 0.0 ± 0.0
Met
2.727MetAla: 2.727 ± 0.05
0.308MetCys: 0.308 ± 0.016
1.908MetAsp: 1.908 ± 0.042
2.941MetGlu: 2.941 ± 0.053
0.998MetPhe: 0.998 ± 0.028
2.347MetGly: 2.347 ± 0.051
0.381MetHis: 0.381 ± 0.017
2.286MetIle: 2.286 ± 0.044
2.627MetLys: 2.627 ± 0.046
2.539MetLeu: 2.539 ± 0.043
1.012MetMet: 1.012 ± 0.031
1.599MetAsn: 1.599 ± 0.034
1.179MetPro: 1.179 ± 0.032
0.855MetGln: 0.855 ± 0.024
1.173MetArg: 1.173 ± 0.031
1.745MetSer: 1.745 ± 0.034
1.779MetThr: 1.779 ± 0.043
2.219MetVal: 2.219 ± 0.043
0.207MetTrp: 0.207 ± 0.012
0.844MetTyr: 0.844 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
2.938AsnAla: 2.938 ± 0.049
0.569AsnCys: 0.569 ± 0.026
1.991AsnAsp: 1.991 ± 0.036
2.624AsnGlu: 2.624 ± 0.047
1.598AsnPhe: 1.598 ± 0.032
3.698AsnGly: 3.698 ± 0.069
0.9AsnHis: 0.9 ± 0.024
3.188AsnIle: 3.188 ± 0.051
2.473AsnLys: 2.473 ± 0.047
3.828AsnLeu: 3.828 ± 0.058
1.29AsnMet: 1.29 ± 0.034
1.92AsnAsn: 1.92 ± 0.036
2.117AsnPro: 2.117 ± 0.036
1.672AsnGln: 1.672 ± 0.039
2.155AsnArg: 2.155 ± 0.045
2.432AsnSer: 2.432 ± 0.043
2.321AsnThr: 2.321 ± 0.047
2.728AsnVal: 2.728 ± 0.049
0.432AsnTrp: 0.432 ± 0.021
1.899AsnTyr: 1.899 ± 0.046
0.0AsnXaa: 0.0 ± 0.0
Pro
2.694ProAla: 2.694 ± 0.056
0.466ProCys: 0.466 ± 0.025
2.427ProAsp: 2.427 ± 0.044
3.246ProGlu: 3.246 ± 0.065
1.628ProPhe: 1.628 ± 0.037
2.76ProGly: 2.76 ± 0.05
0.62ProHis: 0.62 ± 0.024
2.16ProIle: 2.16 ± 0.047
1.842ProLys: 1.842 ± 0.041
3.028ProLeu: 3.028 ± 0.047
1.068ProMet: 1.068 ± 0.039
1.254ProAsn: 1.254 ± 0.03
0.965ProPro: 0.965 ± 0.032
1.215ProGln: 1.215 ± 0.051
1.125ProArg: 1.125 ± 0.034
2.024ProSer: 2.024 ± 0.037
1.651ProThr: 1.651 ± 0.077
3.392ProVal: 3.392 ± 0.109
0.358ProTrp: 0.358 ± 0.017
1.535ProTyr: 1.535 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
2.548GlnAla: 2.548 ± 0.055
0.369GlnCys: 0.369 ± 0.017
1.491GlnAsp: 1.491 ± 0.037
2.672GlnGlu: 2.672 ± 0.05
1.212GlnPhe: 1.212 ± 0.031
2.227GlnGly: 2.227 ± 0.067
0.413GlnHis: 0.413 ± 0.017
2.449GlnIle: 2.449 ± 0.047
2.362GlnLys: 2.362 ± 0.048
2.793GlnLeu: 2.793 ± 0.048
1.23GlnMet: 1.23 ± 0.031
1.5GlnAsn: 1.5 ± 0.033
1.043GlnPro: 1.043 ± 0.079
1.09GlnGln: 1.09 ± 0.03
1.305GlnArg: 1.305 ± 0.036
1.794GlnSer: 1.794 ± 0.032
1.77GlnThr: 1.77 ± 0.038
2.237GlnVal: 2.237 ± 0.042
0.35GlnTrp: 0.35 ± 0.015
1.415GlnTyr: 1.415 ± 0.031
0.0GlnXaa: 0.0 ± 0.0
Arg
2.56ArgAla: 2.56 ± 0.046
0.544ArgCys: 0.544 ± 0.023
2.338ArgAsp: 2.338 ± 0.047
3.808ArgGlu: 3.808 ± 0.062
2.046ArgPhe: 2.046 ± 0.046
2.459ArgGly: 2.459 ± 0.043
0.82ArgHis: 0.82 ± 0.026
3.773ArgIle: 3.773 ± 0.062
3.363ArgLys: 3.363 ± 0.051
4.418ArgLeu: 4.418 ± 0.068
1.643ArgMet: 1.643 ± 0.038
2.176ArgAsn: 2.176 ± 0.035
1.425ArgPro: 1.425 ± 0.035
1.636ArgGln: 1.636 ± 0.04
2.213ArgArg: 2.213 ± 0.046
2.297ArgSer: 2.297 ± 0.037
2.275ArgThr: 2.275 ± 0.043
2.692ArgVal: 2.692 ± 0.044
0.386ArgTrp: 0.386 ± 0.018
1.957ArgTyr: 1.957 ± 0.046
0.0ArgXaa: 0.0 ± 0.0
Ser
4.324SerAla: 4.324 ± 0.065
0.852SerCys: 0.852 ± 0.028
3.134SerAsp: 3.134 ± 0.054
3.645SerGlu: 3.645 ± 0.067
2.933SerPhe: 2.933 ± 0.052
5.636SerGly: 5.636 ± 0.082
1.124SerHis: 1.124 ± 0.028
4.522SerIle: 4.522 ± 0.073
3.235SerLys: 3.235 ± 0.053
5.712SerLeu: 5.712 ± 0.067
2.063SerMet: 2.063 ± 0.041
2.31SerAsn: 2.31 ± 0.043
2.123SerPro: 2.123 ± 0.063
2.056SerGln: 2.056 ± 0.043
3.084SerArg: 3.084 ± 0.049
3.955SerSer: 3.955 ± 0.067
2.873SerThr: 2.873 ± 0.054
4.498SerVal: 4.498 ± 0.062
0.598SerTrp: 0.598 ± 0.021
2.488SerTyr: 2.488 ± 0.047
0.0SerXaa: 0.0 ± 0.0
Thr
4.831ThrAla: 4.831 ± 0.095
0.634ThrCys: 0.634 ± 0.024
2.985ThrAsp: 2.985 ± 0.059
3.57ThrGlu: 3.57 ± 0.057
2.152ThrPhe: 2.152 ± 0.044
5.369ThrGly: 5.369 ± 0.147
0.816ThrHis: 0.816 ± 0.027
3.943ThrIle: 3.943 ± 0.064
2.856ThrLys: 2.856 ± 0.05
4.717ThrLeu: 4.717 ± 0.064
1.565ThrMet: 1.565 ± 0.037
1.979ThrAsn: 1.979 ± 0.046
2.242ThrPro: 2.242 ± 0.044
1.447ThrGln: 1.447 ± 0.054
2.062ThrArg: 2.062 ± 0.041
3.087ThrSer: 3.087 ± 0.047
2.751ThrThr: 2.751 ± 0.06
4.623ThrVal: 4.623 ± 0.074
0.52ThrTrp: 0.52 ± 0.019
2.007ThrTyr: 2.007 ± 0.045
0.0ThrXaa: 0.0 ± 0.0
Val
4.612ValAla: 4.612 ± 0.063
1.094ValCys: 1.094 ± 0.033
3.484ValAsp: 3.484 ± 0.057
4.248ValGlu: 4.248 ± 0.059
3.156ValPhe: 3.156 ± 0.054
4.201ValGly: 4.201 ± 0.078
1.064ValHis: 1.064 ± 0.031
5.27ValIle: 5.27 ± 0.073
4.428ValLys: 4.428 ± 0.059
6.576ValLeu: 6.576 ± 0.086
2.094ValMet: 2.094 ± 0.037
2.943ValAsn: 2.943 ± 0.049
2.646ValPro: 2.646 ± 0.049
1.903ValGln: 1.903 ± 0.034
3.023ValArg: 3.023 ± 0.045
4.843ValSer: 4.843 ± 0.083
4.381ValThr: 4.381 ± 0.084
4.619ValVal: 4.619 ± 0.065
0.622ValTrp: 0.622 ± 0.021
2.573ValTyr: 2.573 ± 0.044
0.0ValXaa: 0.0 ± 0.0
Trp
0.56TrpAla: 0.56 ± 0.022
0.128TrpCys: 0.128 ± 0.01
0.571TrpAsp: 0.571 ± 0.022
0.681TrpGlu: 0.681 ± 0.023
0.416TrpPhe: 0.416 ± 0.017
0.692TrpGly: 0.692 ± 0.027
0.15TrpHis: 0.15 ± 0.012
0.657TrpIle: 0.657 ± 0.021
0.736TrpLys: 0.736 ± 0.026
0.945TrpLeu: 0.945 ± 0.028
0.29TrpMet: 0.29 ± 0.014
0.562TrpAsn: 0.562 ± 0.022
0.228TrpPro: 0.228 ± 0.011
0.362TrpGln: 0.362 ± 0.016
0.389TrpArg: 0.389 ± 0.018
0.504TrpSer: 0.504 ± 0.019
0.478TrpThr: 0.478 ± 0.021
0.565TrpVal: 0.565 ± 0.023
0.116TrpTrp: 0.116 ± 0.01
0.432TrpTyr: 0.432 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.567TyrAla: 2.567 ± 0.041
0.589TyrCys: 0.589 ± 0.019
2.354TyrAsp: 2.354 ± 0.046
2.906TyrGlu: 2.906 ± 0.048
1.88TyrPhe: 1.88 ± 0.04
3.243TyrGly: 3.243 ± 0.059
0.881TyrHis: 0.881 ± 0.026
2.639TyrIle: 2.639 ± 0.046
2.184TyrLys: 2.184 ± 0.041
3.819TyrLeu: 3.819 ± 0.056
1.119TyrMet: 1.119 ± 0.03
1.594TyrAsn: 1.594 ± 0.039
1.497TyrPro: 1.497 ± 0.039
1.594TyrGln: 1.594 ± 0.04
2.068TyrArg: 2.068 ± 0.041
2.401TyrSer: 2.401 ± 0.046
2.226TyrThr: 2.226 ± 0.049
2.435TyrVal: 2.435 ± 0.044
0.375TyrTrp: 0.375 ± 0.017
1.915TyrTyr: 1.915 ± 0.046
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4133 proteins (1343575 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski