Amino acid dipepetide frequency for Staphylococcus phage phi5967PVL

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.428AlaAla: 2.428 ± 0.528
0.09AlaCys: 0.09 ± 0.102
2.968AlaAsp: 2.968 ± 0.425
3.687AlaGlu: 3.687 ± 0.748
2.338AlaPhe: 2.338 ± 0.698
4.406AlaGly: 4.406 ± 0.92
0.719AlaHis: 0.719 ± 0.252
4.676AlaIle: 4.676 ± 0.819
4.586AlaLys: 4.586 ± 0.693
4.856AlaLeu: 4.856 ± 0.682
1.619AlaMet: 1.619 ± 0.416
4.137AlaAsn: 4.137 ± 0.669
1.709AlaPro: 1.709 ± 0.415
2.248AlaGln: 2.248 ± 0.539
2.428AlaArg: 2.428 ± 0.533
2.698AlaSer: 2.698 ± 0.57
3.147AlaThr: 3.147 ± 0.616
3.327AlaVal: 3.327 ± 0.585
0.27AlaTrp: 0.27 ± 0.152
2.968AlaTyr: 2.968 ± 0.529
0.0AlaXaa: 0.0 ± 0.0
Cys
0.27CysAla: 0.27 ± 0.161
0.0CysCys: 0.0 ± 0.0
0.09CysAsp: 0.09 ± 0.085
0.36CysGlu: 0.36 ± 0.227
0.09CysPhe: 0.09 ± 0.093
0.18CysGly: 0.18 ± 0.13
0.09CysHis: 0.09 ± 0.091
0.629CysIle: 0.629 ± 0.268
0.36CysLys: 0.36 ± 0.164
0.18CysLeu: 0.18 ± 0.137
0.0CysMet: 0.0 ± 0.0
0.27CysAsn: 0.27 ± 0.142
0.18CysPro: 0.18 ± 0.128
0.09CysGln: 0.09 ± 0.091
0.18CysArg: 0.18 ± 0.133
0.0CysSer: 0.0 ± 0.0
0.27CysThr: 0.27 ± 0.169
0.36CysVal: 0.36 ± 0.168
0.09CysTrp: 0.09 ± 0.092
0.27CysTyr: 0.27 ± 0.159
0.0CysXaa: 0.0 ± 0.0
Asp
3.058AspAla: 3.058 ± 0.416
0.45AspCys: 0.45 ± 0.186
3.867AspAsp: 3.867 ± 0.995
4.496AspGlu: 4.496 ± 0.934
3.777AspPhe: 3.777 ± 0.543
4.586AspGly: 4.586 ± 0.905
0.809AspHis: 0.809 ± 0.262
4.676AspIle: 4.676 ± 0.502
5.755AspLys: 5.755 ± 0.847
5.755AspLeu: 5.755 ± 0.764
1.799AspMet: 1.799 ± 0.357
3.597AspAsn: 3.597 ± 0.515
1.799AspPro: 1.799 ± 0.432
0.54AspGln: 0.54 ± 0.234
2.248AspArg: 2.248 ± 0.61
3.507AspSer: 3.507 ± 0.662
3.507AspThr: 3.507 ± 0.727
3.597AspVal: 3.597 ± 0.636
0.629AspTrp: 0.629 ± 0.174
2.698AspTyr: 2.698 ± 0.573
0.0AspXaa: 0.0 ± 0.0
Glu
3.597GluAla: 3.597 ± 0.705
0.54GluCys: 0.54 ± 0.22
3.597GluAsp: 3.597 ± 0.684
6.295GluGlu: 6.295 ± 1.101
2.878GluPhe: 2.878 ± 0.508
2.698GluGly: 2.698 ± 0.539
1.349GluHis: 1.349 ± 0.298
5.935GluIle: 5.935 ± 0.792
7.284GluLys: 7.284 ± 1.227
6.745GluLeu: 6.745 ± 0.829
2.338GluMet: 2.338 ± 0.549
4.676GluAsn: 4.676 ± 0.564
1.079GluPro: 1.079 ± 0.321
3.777GluGln: 3.777 ± 0.687
4.317GluArg: 4.317 ± 0.691
3.957GluSer: 3.957 ± 0.617
3.867GluThr: 3.867 ± 0.835
4.406GluVal: 4.406 ± 0.611
1.079GluTrp: 1.079 ± 0.325
4.496GluTyr: 4.496 ± 0.805
0.0GluXaa: 0.0 ± 0.0
Phe
2.338PheAla: 2.338 ± 0.588
0.09PheCys: 0.09 ± 0.102
2.068PheAsp: 2.068 ± 0.353
2.878PheGlu: 2.878 ± 0.496
1.259PhePhe: 1.259 ± 0.357
2.698PheGly: 2.698 ± 0.542
0.54PheHis: 0.54 ± 0.201
4.496PheIle: 4.496 ± 0.661
3.957PheLys: 3.957 ± 0.683
2.608PheLeu: 2.608 ± 0.448
1.349PheMet: 1.349 ± 0.384
4.047PheAsn: 4.047 ± 0.664
0.989PhePro: 0.989 ± 0.327
0.719PheGln: 0.719 ± 0.21
1.978PheArg: 1.978 ± 0.456
2.968PheSer: 2.968 ± 0.594
2.698PheThr: 2.698 ± 0.57
2.518PheVal: 2.518 ± 0.493
0.18PheTrp: 0.18 ± 0.145
1.259PheTyr: 1.259 ± 0.353
0.0PheXaa: 0.0 ± 0.0
Gly
2.878GlyAla: 2.878 ± 0.581
0.36GlyCys: 0.36 ± 0.182
3.417GlyAsp: 3.417 ± 0.565
4.047GlyGlu: 4.047 ± 0.799
2.788GlyPhe: 2.788 ± 0.571
5.216GlyGly: 5.216 ± 1.067
1.349GlyHis: 1.349 ± 0.413
3.597GlyIle: 3.597 ± 0.885
7.464GlyLys: 7.464 ± 1.18
5.126GlyLeu: 5.126 ± 0.97
1.169GlyMet: 1.169 ± 0.344
3.597GlyAsn: 3.597 ± 0.659
1.349GlyPro: 1.349 ± 0.331
2.338GlyGln: 2.338 ± 0.639
2.338GlyArg: 2.338 ± 0.489
3.687GlySer: 3.687 ± 0.58
2.878GlyThr: 2.878 ± 0.676
4.406GlyVal: 4.406 ± 0.81
0.899GlyTrp: 0.899 ± 0.301
3.147GlyTyr: 3.147 ± 0.629
0.0GlyXaa: 0.0 ± 0.0
His
1.439HisAla: 1.439 ± 0.4
0.0HisCys: 0.0 ± 0.0
1.169HisAsp: 1.169 ± 0.464
1.259HisGlu: 1.259 ± 0.373
0.989HisPhe: 0.989 ± 0.272
0.629HisGly: 0.629 ± 0.303
0.45HisHis: 0.45 ± 0.179
1.799HisIle: 1.799 ± 0.357
1.439HisLys: 1.439 ± 0.454
0.899HisLeu: 0.899 ± 0.236
0.0HisMet: 0.0 ± 0.0
1.259HisAsn: 1.259 ± 0.402
0.629HisPro: 0.629 ± 0.226
0.54HisGln: 0.54 ± 0.182
0.27HisArg: 0.27 ± 0.144
0.809HisSer: 0.809 ± 0.207
1.619HisThr: 1.619 ± 0.333
1.259HisVal: 1.259 ± 0.365
0.27HisTrp: 0.27 ± 0.173
0.899HisTyr: 0.899 ± 0.295
0.0HisXaa: 0.0 ± 0.0
Ile
5.755IleAla: 5.755 ± 0.704
0.27IleCys: 0.27 ± 0.138
4.496IleAsp: 4.496 ± 0.637
6.385IleGlu: 6.385 ± 0.654
3.777IlePhe: 3.777 ± 0.58
4.586IleGly: 4.586 ± 0.614
1.709IleHis: 1.709 ± 0.49
4.496IleIle: 4.496 ± 0.959
8.633IleLys: 8.633 ± 0.794
3.957IleLeu: 3.957 ± 0.593
1.079IleMet: 1.079 ± 0.307
5.935IleAsn: 5.935 ± 0.966
2.158IlePro: 2.158 ± 0.394
3.327IleGln: 3.327 ± 0.496
2.968IleArg: 2.968 ± 0.56
4.676IleSer: 4.676 ± 0.549
5.216IleThr: 5.216 ± 0.611
4.496IleVal: 4.496 ± 0.608
1.079IleTrp: 1.079 ± 0.525
2.518IleTyr: 2.518 ± 0.659
0.0IleXaa: 0.0 ± 0.0
Lys
6.655LysAla: 6.655 ± 0.897
0.36LysCys: 0.36 ± 0.2
6.924LysAsp: 6.924 ± 0.632
8.363LysGlu: 8.363 ± 1.061
3.058LysPhe: 3.058 ± 0.474
6.205LysGly: 6.205 ± 1.022
1.079LysHis: 1.079 ± 0.354
6.385LysIle: 6.385 ± 0.945
7.734LysLys: 7.734 ± 0.969
7.374LysLeu: 7.374 ± 0.784
2.608LysMet: 2.608 ± 0.652
6.205LysAsn: 6.205 ± 0.893
3.147LysPro: 3.147 ± 0.627
3.777LysGln: 3.777 ± 0.547
4.856LysArg: 4.856 ± 0.731
4.766LysSer: 4.766 ± 0.846
4.946LysThr: 4.946 ± 0.768
6.205LysVal: 6.205 ± 0.801
1.619LysTrp: 1.619 ± 0.4
4.496LysTyr: 4.496 ± 0.64
0.0LysXaa: 0.0 ± 0.0
Leu
3.507LeuAla: 3.507 ± 0.631
0.54LeuCys: 0.54 ± 0.23
5.036LeuAsp: 5.036 ± 0.738
6.205LeuGlu: 6.205 ± 0.907
3.058LeuPhe: 3.058 ± 0.47
3.777LeuGly: 3.777 ± 0.647
1.259LeuHis: 1.259 ± 0.288
5.486LeuIle: 5.486 ± 0.734
7.644LeuLys: 7.644 ± 0.86
5.126LeuLeu: 5.126 ± 0.801
1.529LeuMet: 1.529 ± 0.462
6.025LeuAsn: 6.025 ± 0.918
2.608LeuPro: 2.608 ± 0.59
2.788LeuGln: 2.788 ± 0.451
3.058LeuArg: 3.058 ± 0.772
5.306LeuSer: 5.306 ± 0.757
4.137LeuThr: 4.137 ± 0.768
3.777LeuVal: 3.777 ± 0.696
0.809LeuTrp: 0.809 ± 0.264
3.597LeuTyr: 3.597 ± 0.725
0.0LeuXaa: 0.0 ± 0.0
Met
1.079MetAla: 1.079 ± 0.406
0.09MetCys: 0.09 ± 0.094
1.529MetAsp: 1.529 ± 0.384
1.079MetGlu: 1.079 ± 0.361
0.809MetPhe: 0.809 ± 0.208
1.169MetGly: 1.169 ± 0.542
0.719MetHis: 0.719 ± 0.313
2.608MetIle: 2.608 ± 0.475
2.158MetLys: 2.158 ± 0.528
1.709MetLeu: 1.709 ± 0.315
0.54MetMet: 0.54 ± 0.269
2.248MetAsn: 2.248 ± 0.555
0.45MetPro: 0.45 ± 0.223
0.899MetGln: 0.899 ± 0.291
1.079MetArg: 1.079 ± 0.336
1.619MetSer: 1.619 ± 0.406
0.809MetThr: 0.809 ± 0.216
1.079MetVal: 1.079 ± 0.225
0.36MetTrp: 0.36 ± 0.161
0.54MetTyr: 0.54 ± 0.252
0.0MetXaa: 0.0 ± 0.0
Asn
3.687AsnAla: 3.687 ± 0.65
0.09AsnCys: 0.09 ± 0.085
3.777AsnAsp: 3.777 ± 0.58
5.665AsnGlu: 5.665 ± 0.953
2.338AsnPhe: 2.338 ± 0.691
5.665AsnGly: 5.665 ± 0.6
0.989AsnHis: 0.989 ± 0.36
5.576AsnIle: 5.576 ± 0.574
7.374AsnLys: 7.374 ± 0.955
4.496AsnLeu: 4.496 ± 0.855
1.799AsnMet: 1.799 ± 0.323
5.576AsnAsn: 5.576 ± 1.029
2.968AsnPro: 2.968 ± 0.353
3.327AsnGln: 3.327 ± 0.503
3.237AsnArg: 3.237 ± 0.572
3.597AsnSer: 3.597 ± 0.531
3.687AsnThr: 3.687 ± 0.455
4.406AsnVal: 4.406 ± 0.736
1.439AsnTrp: 1.439 ± 0.595
3.237AsnTyr: 3.237 ± 0.537
0.0AsnXaa: 0.0 ± 0.0
Pro
0.719ProAla: 0.719 ± 0.273
0.0ProCys: 0.0 ± 0.0
1.169ProAsp: 1.169 ± 0.309
2.428ProGlu: 2.428 ± 0.471
1.349ProPhe: 1.349 ± 0.398
0.989ProGly: 0.989 ± 0.286
0.45ProHis: 0.45 ± 0.176
2.338ProIle: 2.338 ± 0.545
2.878ProLys: 2.878 ± 0.657
2.608ProLeu: 2.608 ± 0.61
0.719ProMet: 0.719 ± 0.242
1.888ProAsn: 1.888 ± 0.347
1.079ProPro: 1.079 ± 0.283
0.809ProGln: 0.809 ± 0.217
1.259ProArg: 1.259 ± 0.316
1.888ProSer: 1.888 ± 0.441
1.709ProThr: 1.709 ± 0.497
1.439ProVal: 1.439 ± 0.373
0.27ProTrp: 0.27 ± 0.14
1.349ProTyr: 1.349 ± 0.342
0.0ProXaa: 0.0 ± 0.0
Gln
2.878GlnAla: 2.878 ± 0.566
0.18GlnCys: 0.18 ± 0.136
2.338GlnAsp: 2.338 ± 0.609
2.428GlnGlu: 2.428 ± 0.487
1.349GlnPhe: 1.349 ± 0.344
1.709GlnGly: 1.709 ± 0.439
0.989GlnHis: 0.989 ± 0.298
3.597GlnIle: 3.597 ± 0.681
2.968GlnLys: 2.968 ± 0.492
3.147GlnLeu: 3.147 ± 0.491
0.629GlnMet: 0.629 ± 0.225
3.237GlnAsn: 3.237 ± 0.54
1.079GlnPro: 1.079 ± 0.346
1.799GlnGln: 1.799 ± 0.469
2.338GlnArg: 2.338 ± 0.384
2.158GlnSer: 2.158 ± 0.429
1.799GlnThr: 1.799 ± 0.501
1.978GlnVal: 1.978 ± 0.323
0.36GlnTrp: 0.36 ± 0.173
1.259GlnTyr: 1.259 ± 0.372
0.0GlnXaa: 0.0 ± 0.0
Arg
2.068ArgAla: 2.068 ± 0.417
0.27ArgCys: 0.27 ± 0.158
2.788ArgAsp: 2.788 ± 0.403
3.867ArgGlu: 3.867 ± 0.715
1.709ArgPhe: 1.709 ± 0.385
2.068ArgGly: 2.068 ± 0.396
0.719ArgHis: 0.719 ± 0.229
3.867ArgIle: 3.867 ± 0.64
4.317ArgLys: 4.317 ± 0.61
3.417ArgLeu: 3.417 ± 0.515
0.809ArgMet: 0.809 ± 0.247
3.058ArgAsn: 3.058 ± 0.511
0.629ArgPro: 0.629 ± 0.271
1.619ArgGln: 1.619 ± 0.47
2.068ArgArg: 2.068 ± 0.412
1.619ArgSer: 1.619 ± 0.338
2.698ArgThr: 2.698 ± 0.67
2.428ArgVal: 2.428 ± 0.411
0.629ArgTrp: 0.629 ± 0.196
2.788ArgTyr: 2.788 ± 0.69
0.0ArgXaa: 0.0 ± 0.0
Ser
3.417SerAla: 3.417 ± 0.671
0.18SerCys: 0.18 ± 0.139
4.317SerAsp: 4.317 ± 0.648
4.676SerGlu: 4.676 ± 0.679
3.058SerPhe: 3.058 ± 0.664
4.137SerGly: 4.137 ± 0.835
0.989SerHis: 0.989 ± 0.239
4.676SerIle: 4.676 ± 0.667
4.676SerLys: 4.676 ± 0.772
3.327SerLeu: 3.327 ± 0.519
1.169SerMet: 1.169 ± 0.268
4.766SerAsn: 4.766 ± 0.611
0.54SerPro: 0.54 ± 0.244
2.968SerGln: 2.968 ± 0.443
1.799SerArg: 1.799 ± 0.415
3.687SerSer: 3.687 ± 0.585
2.698SerThr: 2.698 ± 0.468
2.608SerVal: 2.608 ± 0.456
0.629SerTrp: 0.629 ± 0.303
2.608SerTyr: 2.608 ± 0.586
0.0SerXaa: 0.0 ± 0.0
Thr
3.597ThrAla: 3.597 ± 0.637
0.18ThrCys: 0.18 ± 0.141
3.417ThrAsp: 3.417 ± 0.828
3.147ThrGlu: 3.147 ± 0.48
2.068ThrPhe: 2.068 ± 0.367
4.047ThrGly: 4.047 ± 1.025
1.619ThrHis: 1.619 ± 0.424
3.327ThrIle: 3.327 ± 0.606
4.766ThrLys: 4.766 ± 0.839
4.856ThrLeu: 4.856 ± 0.561
0.629ThrMet: 0.629 ± 0.229
3.147ThrAsn: 3.147 ± 0.73
2.878ThrPro: 2.878 ± 0.407
1.439ThrGln: 1.439 ± 0.406
2.158ThrArg: 2.158 ± 0.394
3.597ThrSer: 3.597 ± 0.739
3.507ThrThr: 3.507 ± 0.673
4.317ThrVal: 4.317 ± 0.677
0.719ThrTrp: 0.719 ± 0.209
2.518ThrTyr: 2.518 ± 0.572
0.0ThrXaa: 0.0 ± 0.0
Val
3.507ValAla: 3.507 ± 0.691
0.09ValCys: 0.09 ± 0.098
4.406ValAsp: 4.406 ± 0.786
3.687ValGlu: 3.687 ± 0.59
2.068ValPhe: 2.068 ± 0.567
3.237ValGly: 3.237 ± 0.612
1.079ValHis: 1.079 ± 0.355
4.586ValIle: 4.586 ± 0.772
6.745ValLys: 6.745 ± 0.713
4.317ValLeu: 4.317 ± 0.547
1.349ValMet: 1.349 ± 0.319
4.406ValAsn: 4.406 ± 0.594
1.259ValPro: 1.259 ± 0.293
2.518ValGln: 2.518 ± 0.589
2.428ValArg: 2.428 ± 0.493
3.417ValSer: 3.417 ± 0.615
3.867ValThr: 3.867 ± 0.581
3.867ValVal: 3.867 ± 0.714
0.719ValTrp: 0.719 ± 0.263
2.068ValTyr: 2.068 ± 0.398
0.0ValXaa: 0.0 ± 0.0
Trp
0.45TrpAla: 0.45 ± 0.209
0.0TrpCys: 0.0 ± 0.0
0.809TrpAsp: 0.809 ± 0.257
0.629TrpGlu: 0.629 ± 0.25
0.989TrpPhe: 0.989 ± 0.293
0.899TrpGly: 0.899 ± 0.303
0.0TrpHis: 0.0 ± 0.0
1.349TrpIle: 1.349 ± 0.31
1.169TrpLys: 1.169 ± 0.259
1.349TrpLeu: 1.349 ± 0.292
0.54TrpMet: 0.54 ± 0.212
1.169TrpAsn: 1.169 ± 0.366
0.18TrpPro: 0.18 ± 0.113
0.629TrpGln: 0.629 ± 0.186
0.45TrpArg: 0.45 ± 0.204
0.27TrpSer: 0.27 ± 0.151
0.36TrpThr: 0.36 ± 0.147
0.899TrpVal: 0.899 ± 0.278
0.09TrpTrp: 0.09 ± 0.08
0.54TrpTyr: 0.54 ± 0.195
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.978TyrAla: 1.978 ± 0.307
0.18TyrCys: 0.18 ± 0.139
2.968TyrAsp: 2.968 ± 0.684
3.327TyrGlu: 3.327 ± 0.679
1.888TyrPhe: 1.888 ± 0.471
3.147TyrGly: 3.147 ± 0.65
0.719TyrHis: 0.719 ± 0.302
3.417TyrIle: 3.417 ± 0.688
4.856TyrLys: 4.856 ± 0.764
3.507TyrLeu: 3.507 ± 0.736
0.809TyrMet: 0.809 ± 0.277
3.687TyrAsn: 3.687 ± 0.519
0.629TyrPro: 0.629 ± 0.207
2.068TyrGln: 2.068 ± 0.438
1.978TyrArg: 1.978 ± 0.438
2.698TyrSer: 2.698 ± 0.419
2.518TyrThr: 2.518 ± 0.468
2.248TyrVal: 2.248 ± 0.498
0.629TyrTrp: 0.629 ± 0.209
0.809TyrTyr: 0.809 ± 0.264
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 42 proteins (11121 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski