Lactococcus lactis phage p272

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Skunavirus; Lactococcus virus p272

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9R2C3|R9R2C3_9CAUD Uncharacterized protein OS=Lactococcus lactis phage p272 OX=213777 GN=P272_0014 PE=4 SV=1
MM1 pKa = 7.56NEE3 pKa = 3.62FDD5 pKa = 6.3SYY7 pKa = 11.39IDD9 pKa = 3.33WYY11 pKa = 11.58NNLLTMPLNDD21 pKa = 3.86VILGVKK27 pKa = 8.96DD28 pKa = 3.57TIEE31 pKa = 4.55DD32 pKa = 3.52KK33 pKa = 10.66TVYY36 pKa = 10.62LSLSDD41 pKa = 3.89SKK43 pKa = 9.65VTKK46 pKa = 9.7MDD48 pKa = 3.05NTSFVMGYY56 pKa = 9.13YY57 pKa = 8.74YY58 pKa = 10.45QVVLSVKK65 pKa = 10.52DD66 pKa = 3.51VDD68 pKa = 4.41DD69 pKa = 4.44EE70 pKa = 4.52LVGLVGDD77 pKa = 4.15VLQNGWNMTNWSEE90 pKa = 4.32NSHH93 pKa = 6.58LYY95 pKa = 10.7NYY97 pKa = 8.61TGTVYY102 pKa = 10.42LPCGAGGQAWQQ113 pKa = 3.59

Molecular weight: 12.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9R1A4|R9R1A4_9CAUD Putative tape-measure protein OS=Lactococcus lactis phage p272 OX=213777 GN=P272_0015 PE=4 SV=1
MM1 pKa = 7.87AMNLLNTSSIAKK13 pKa = 9.58EE14 pKa = 3.88MQTKK18 pKa = 7.47VTEE21 pKa = 4.24RR22 pKa = 11.84MGDD25 pKa = 2.9WFEE28 pKa = 5.66AEE30 pKa = 4.33FKK32 pKa = 11.0ARR34 pKa = 11.84ANSASRR40 pKa = 11.84RR41 pKa = 11.84TRR43 pKa = 11.84SIRR46 pKa = 11.84SHH48 pKa = 4.55GHH50 pKa = 5.13TYY52 pKa = 9.85TYY54 pKa = 11.35ARR56 pKa = 11.84YY57 pKa = 9.69QNTGQLSSNLKK68 pKa = 8.86QVKK71 pKa = 9.97KK72 pKa = 10.41GDD74 pKa = 4.02KK75 pKa = 9.89IVVNAGTRR83 pKa = 11.84ANYY86 pKa = 8.51TSGYY90 pKa = 8.75HH91 pKa = 6.14GMYY94 pKa = 10.43FLVEE98 pKa = 3.87KK99 pKa = 10.67RR100 pKa = 11.84GMQEE104 pKa = 3.8VKK106 pKa = 8.23TTLKK110 pKa = 10.66KK111 pKa = 10.03GANYY115 pKa = 9.69ANSMKK120 pKa = 10.65LL121 pKa = 3.26

Molecular weight: 13.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

9503

42

916

155.8

17.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.798 ± 0.59

0.737 ± 0.153

5.682 ± 0.241

7.703 ± 0.638

4.251 ± 0.338

6.093 ± 0.661

1.431 ± 0.195

6.987 ± 0.311

9.46 ± 0.55

8.66 ± 0.448

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.599 ± 0.157

6.987 ± 0.355

2.178 ± 0.262

3.462 ± 0.235

3.641 ± 0.294

6.009 ± 0.473

6.156 ± 0.306

6.24 ± 0.326

1.526 ± 0.162

4.399 ± 0.384
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski