Dolphin morbillivirus
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q709E7|Q709E7_9MONO Isoform of Q709E8 Protein C OS=Dolphin morbillivirus OX=37131 GN=P/V/C PE=3 SV=1
MM1 pKa = 8.2 AEE3 pKa = 3.69 EE4 pKa = 3.77 QAYY7 pKa = 9.6 HH8 pKa = 5.39 VNKK11 pKa = 10.43 GLEE14 pKa = 4.24 CLKK17 pKa = 10.54 SLRR20 pKa = 11.84 EE21 pKa = 4.01 NPPDD25 pKa = 3.34 AVEE28 pKa = 4.02 IKK30 pKa = 9.19 EE31 pKa = 4.04 AQIIRR36 pKa = 11.84 SKK38 pKa = 10.59 AACEE42 pKa = 3.9 EE43 pKa = 4.48 SSEE46 pKa = 4.62 SHH48 pKa = 6.35 HH49 pKa = 6.42 QDD51 pKa = 2.75 NSEE54 pKa = 4.0 KK55 pKa = 10.26 DD56 pKa = 3.33 TLDD59 pKa = 3.48 FDD61 pKa = 4.91 EE62 pKa = 5.34 SCSSAIRR69 pKa = 11.84 PEE71 pKa = 4.25 TYY73 pKa = 10.75 RR74 pKa = 11.84 MLLGDD79 pKa = 3.81 DD80 pKa = 3.56 TGFRR84 pKa = 11.84 APGYY88 pKa = 9.42 IPNEE92 pKa = 4.26 GEE94 pKa = 4.5 PEE96 pKa = 4.25 PGDD99 pKa = 3.31 IGKK102 pKa = 9.73 EE103 pKa = 4.0 EE104 pKa = 3.99 PAVRR108 pKa = 11.84 CYY110 pKa = 10.36 HH111 pKa = 6.32 VYY113 pKa = 10.91 DD114 pKa = 3.91 HH115 pKa = 7.16 GGQAVEE121 pKa = 4.43 GVKK124 pKa = 10.73 DD125 pKa = 3.52 ADD127 pKa = 4.03 LLVVPTGSDD136 pKa = 3.16 DD137 pKa = 3.79 DD138 pKa = 4.98 AEE140 pKa = 4.27 FRR142 pKa = 11.84 DD143 pKa = 3.97 GDD145 pKa = 3.98 EE146 pKa = 4.88 SSLEE150 pKa = 3.98 SDD152 pKa = 4.12 GEE154 pKa = 4.36 SGTVDD159 pKa = 2.92 TRR161 pKa = 11.84 GNSSSNRR168 pKa = 11.84 GSAPRR173 pKa = 11.84 IKK175 pKa = 10.39 VEE177 pKa = 3.84 RR178 pKa = 11.84 SSDD181 pKa = 3.54 VEE183 pKa = 4.48 TISSEE188 pKa = 3.96 EE189 pKa = 3.78 LQGLIRR195 pKa = 11.84 SQSQKK200 pKa = 10.79 HH201 pKa = 5.53 NGFGVDD207 pKa = 3.67 RR208 pKa = 11.84 FLKK211 pKa = 10.41 VPPIPTSVPLDD222 pKa = 3.77 PAPKK226 pKa = 9.79 SIKK229 pKa = 10.06 KK230 pKa = 8.63 GTGEE234 pKa = 3.87 RR235 pKa = 11.84 SALSGTEE242 pKa = 4.17 TEE244 pKa = 4.72 FSLTGGATRR253 pKa = 11.84 LAQEE257 pKa = 4.41 SRR259 pKa = 11.84 WASSEE264 pKa = 3.81 SSAPAEE270 pKa = 4.01 NVRR273 pKa = 11.84 QSVTNAEE280 pKa = 4.33 RR281 pKa = 11.84 TQKK284 pKa = 10.2 PPQGSGTTASQKK296 pKa = 9.89 SQNNGHH302 pKa = 6.43 SDD304 pKa = 3.78 DD305 pKa = 4.4 EE306 pKa = 4.9 YY307 pKa = 10.86 EE308 pKa = 5.29 DD309 pKa = 3.81 EE310 pKa = 5.99 LFMEE314 pKa = 4.39 VQEE317 pKa = 4.41 IKK319 pKa = 9.85 TAITKK324 pKa = 10.11 INEE327 pKa = 4.11 DD328 pKa = 3.36 NQQIISKK335 pKa = 9.57 LDD337 pKa = 3.47 SIMLLKK343 pKa = 11.11 GEE345 pKa = 4.28 IEE347 pKa = 4.43 SIKK350 pKa = 10.69 KK351 pKa = 9.98 QINKK355 pKa = 9.55 QNITISTIEE364 pKa = 3.93 GHH366 pKa = 6.68 LSSIMIAIPGFGKK379 pKa = 10.48 DD380 pKa = 4.04 PNDD383 pKa = 3.34 PTADD387 pKa = 3.42 VEE389 pKa = 4.6 LNPDD393 pKa = 3.78 LRR395 pKa = 11.84 PIISRR400 pKa = 11.84 DD401 pKa = 3.08 AGRR404 pKa = 11.84 ALAEE408 pKa = 4.11 VLKK411 pKa = 10.81 RR412 pKa = 11.84 PAVEE416 pKa = 4.31 RR417 pKa = 11.84 NPKK420 pKa = 8.29 VTPKK424 pKa = 10.06 VHH426 pKa = 6.85 PGSKK430 pKa = 10.19 GQILRR435 pKa = 11.84 DD436 pKa = 3.79 LQLKK440 pKa = 9.54 PVDD443 pKa = 3.85 RR444 pKa = 11.84 KK445 pKa = 9.68 MSSAVGFVPTDD456 pKa = 3.69 DD457 pKa = 4.04 LPSRR461 pKa = 11.84 SVLRR465 pKa = 11.84 SMIKK469 pKa = 10.12 SSNLEE474 pKa = 4.19 SEE476 pKa = 5.09 HH477 pKa = 6.02 KK478 pKa = 10.34 RR479 pKa = 11.84 SMIGLLNDD487 pKa = 3.4 VKK489 pKa = 10.82 SGKK492 pKa = 9.91 DD493 pKa = 3.06 LGEE496 pKa = 4.3 FYY498 pKa = 11.4 QMVKK502 pKa = 10.53 KK503 pKa = 10.62 IIKK506 pKa = 9.68
Molecular weight: 55.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.08
IPC2_protein 5.029
IPC_protein 4.978
Toseland 4.889
ProMoST 5.105
Dawson 4.952
Bjellqvist 5.092
Wikipedia 4.813
Rodwell 4.863
Grimsley 4.813
Solomon 4.952
Lehninger 4.902
Nozaki 5.067
DTASelect 5.207
Thurlkill 4.889
EMBOSS 4.838
Sillero 5.143
Patrickios 4.431
IPC_peptide 4.965
IPC2_peptide 5.13
IPC2.peptide.svr19 5.14
Protein with the highest isoelectric point:
>tr|Q709E7|Q709E7_9MONO Isoform of Q709E8 Protein C OS=Dolphin morbillivirus OX=37131 GN=P/V/C PE=3 SV=1
MM1 pKa = 7.87 SIRR4 pKa = 11.84 DD5 pKa = 3.67 LSVSNLSEE13 pKa = 4.76 KK14 pKa = 9.82 IRR16 pKa = 11.84 PMLSKK21 pKa = 10.39 LRR23 pKa = 11.84 KK24 pKa = 8.57 PKK26 pKa = 10.4 LSEE29 pKa = 3.87 ARR31 pKa = 11.84 PPAKK35 pKa = 10.25 NQARR39 pKa = 11.84 VITRR43 pKa = 11.84 TTPKK47 pKa = 9.19 KK48 pKa = 9.13 TLLISTNHH56 pKa = 7.04 ALQQLDD62 pKa = 3.78 QKK64 pKa = 10.22 RR65 pKa = 11.84 TACYY69 pKa = 10.33 LVMIQDD75 pKa = 4.76 LEE77 pKa = 4.4 HH78 pKa = 6.22 QVTSLMKK85 pKa = 10.18 EE86 pKa = 4.2 SPSQEE91 pKa = 3.54 TSEE94 pKa = 4.39 RR95 pKa = 11.84 RR96 pKa = 11.84 NLQYY100 pKa = 11.05 DD101 pKa = 3.37 VTMFMITAVKK111 pKa = 9.79 RR112 pKa = 11.84 LKK114 pKa = 9.95 EE115 pKa = 4.1 SRR117 pKa = 11.84 MLTCSWFQQAVMMMQNSEE135 pKa = 4.15 TEE137 pKa = 3.8 MRR139 pKa = 11.84 ALSRR143 pKa = 11.84 AMVNLALLIPEE154 pKa = 5.39 EE155 pKa = 4.16 ILPLTGDD162 pKa = 4.7 LLPGLRR168 pKa = 11.84 SRR170 pKa = 11.84 DD171 pKa = 3.51 RR172 pKa = 11.84 LTLRR176 pKa = 11.84 LL177 pKa = 3.72
Molecular weight: 20.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.213
IPC2_protein 9.341
IPC_protein 9.502
Toseland 10.482
ProMoST 10.014
Dawson 10.57
Bjellqvist 10.189
Wikipedia 10.701
Rodwell 10.95
Grimsley 10.599
Solomon 10.628
Lehninger 10.613
Nozaki 10.467
DTASelect 10.175
Thurlkill 10.467
EMBOSS 10.862
Sillero 10.482
Patrickios 10.701
IPC_peptide 10.643
IPC2_peptide 8.829
IPC2.peptide.svr19 8.487
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
1
7
4880
177
2183
697.1
78.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.799 ± 0.518
1.701 ± 0.295
5.369 ± 0.515
5.84 ± 0.625
3.238 ± 0.368
6.209 ± 0.419
2.152 ± 0.465
7.951 ± 0.366
5.594 ± 0.257
10.369 ± 0.712
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.623 ± 0.271
4.488 ± 0.189
4.508 ± 0.444
3.299 ± 0.31
5.758 ± 0.303
8.443 ± 0.504
5.758 ± 0.212
6.25 ± 0.495
0.922 ± 0.189
3.73 ± 0.634
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here