Parthenium leaf curl alphasatellite
Average proteome isoelectric point is 7.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A143WDM6|A0A143WDM6_9VIRU Replication associated protein OS=Parthenium leaf curl alphasatellite OX=1755198 GN=alpha Rep PE=4 SV=1
MM1 pKa = 7.74 KK2 pKa = 10.4 EE3 pKa = 3.83 EE4 pKa = 4.23 TRR6 pKa = 11.84 VSGPFEE12 pKa = 4.33 FGDD15 pKa = 3.87 YY16 pKa = 10.5 CPSGSHH22 pKa = 6.46 KK23 pKa = 10.13 RR24 pKa = 11.84 RR25 pKa = 11.84 QRR27 pKa = 11.84 EE28 pKa = 3.79 LVIRR32 pKa = 11.84 SPVRR36 pKa = 11.84 MAEE39 pKa = 4.07 EE40 pKa = 4.09 NPSVFRR46 pKa = 11.84 RR47 pKa = 11.84 VKK49 pKa = 10.51 AKK51 pKa = 10.22 LAEE54 pKa = 4.11 EE55 pKa = 4.52 EE56 pKa = 4.33 FQKK59 pKa = 10.72 SAPEE63 pKa = 3.59 IQIRR67 pKa = 11.84 NLKK70 pKa = 9.41 SWQLRR75 pKa = 11.84 LKK77 pKa = 9.21 TLLGRR82 pKa = 11.84 DD83 pKa = 3.56 PDD85 pKa = 3.66 DD86 pKa = 3.61 RR87 pKa = 11.84 TIFWVYY93 pKa = 10.74 GPTGGEE99 pKa = 4.17 GKK101 pKa = 8.47 STFARR106 pKa = 11.84 DD107 pKa = 3.97 LYY109 pKa = 11.14 RR110 pKa = 11.84 SGGWFYY116 pKa = 11.09 TRR118 pKa = 11.84 GGSADD123 pKa = 3.36 NVAYY127 pKa = 10.18 QYY129 pKa = 10.65 IGCLGNNIVFDD140 pKa = 3.87 IPRR143 pKa = 11.84 DD144 pKa = 3.6 KK145 pKa = 10.91 KK146 pKa = 11.07 DD147 pKa = 3.31 YY148 pKa = 10.37 LQYY151 pKa = 11.61 SLIEE155 pKa = 4.03 MFKK158 pKa = 11.15 DD159 pKa = 3.29 RR160 pKa = 11.84 LIVSNKK166 pKa = 9.63 YY167 pKa = 9.28 EE168 pKa = 4.23 PLMAPLVNCIHH179 pKa = 5.92 VVVMSNFLPDD189 pKa = 3.69 FEE191 pKa = 6.43 KK192 pKa = 10.53 ISSDD196 pKa = 3.08 RR197 pKa = 11.84 VHH199 pKa = 7.2 VIPCIPCGVCRR210 pKa = 11.84 RR211 pKa = 11.84 HH212 pKa = 6.55 HH213 pKa = 5.17 ITEE216 pKa = 4.48 IKK218 pKa = 10.37 CEE220 pKa = 4.08 EE221 pKa = 4.3 YY222 pKa = 11.22 VDD224 pKa = 3.7
Molecular weight: 25.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.368
IPC2_protein 7.673
IPC_protein 7.571
Toseland 7.161
ProMoST 8.068
Dawson 8.244
Bjellqvist 8.624
Wikipedia 8.17
Rodwell 8.258
Grimsley 7.161
Solomon 8.346
Lehninger 8.361
Nozaki 8.829
DTASelect 8.346
Thurlkill 8.39
EMBOSS 8.463
Sillero 8.726
Patrickios 4.342
IPC_peptide 8.346
IPC2_peptide 7.688
IPC2.peptide.svr19 7.749
Protein with the highest isoelectric point:
>tr|A0A143WDM6|A0A143WDM6_9VIRU Replication associated protein OS=Parthenium leaf curl alphasatellite OX=1755198 GN=alpha Rep PE=4 SV=1
MM1 pKa = 7.74 KK2 pKa = 10.4 EE3 pKa = 3.83 EE4 pKa = 4.23 TRR6 pKa = 11.84 VSGPFEE12 pKa = 4.33 FGDD15 pKa = 3.87 YY16 pKa = 10.5 CPSGSHH22 pKa = 6.46 KK23 pKa = 10.13 RR24 pKa = 11.84 RR25 pKa = 11.84 QRR27 pKa = 11.84 EE28 pKa = 3.79 LVIRR32 pKa = 11.84 SPVRR36 pKa = 11.84 MAEE39 pKa = 4.07 EE40 pKa = 4.09 NPSVFRR46 pKa = 11.84 RR47 pKa = 11.84 VKK49 pKa = 10.51 AKK51 pKa = 10.22 LAEE54 pKa = 4.11 EE55 pKa = 4.52 EE56 pKa = 4.33 FQKK59 pKa = 10.72 SAPEE63 pKa = 3.59 IQIRR67 pKa = 11.84 NLKK70 pKa = 9.41 SWQLRR75 pKa = 11.84 LKK77 pKa = 9.21 TLLGRR82 pKa = 11.84 DD83 pKa = 3.56 PDD85 pKa = 3.66 DD86 pKa = 3.61 RR87 pKa = 11.84 TIFWVYY93 pKa = 10.74 GPTGGEE99 pKa = 4.17 GKK101 pKa = 8.47 STFARR106 pKa = 11.84 DD107 pKa = 3.97 LYY109 pKa = 11.14 RR110 pKa = 11.84 SGGWFYY116 pKa = 11.09 TRR118 pKa = 11.84 GGSADD123 pKa = 3.36 NVAYY127 pKa = 10.18 QYY129 pKa = 10.65 IGCLGNNIVFDD140 pKa = 3.87 IPRR143 pKa = 11.84 DD144 pKa = 3.6 KK145 pKa = 10.91 KK146 pKa = 11.07 DD147 pKa = 3.31 YY148 pKa = 10.37 LQYY151 pKa = 11.61 SLIEE155 pKa = 4.03 MFKK158 pKa = 11.15 DD159 pKa = 3.29 RR160 pKa = 11.84 LIVSNKK166 pKa = 9.63 YY167 pKa = 9.28 EE168 pKa = 4.23 PLMAPLVNCIHH179 pKa = 5.92 VVVMSNFLPDD189 pKa = 3.69 FEE191 pKa = 6.43 KK192 pKa = 10.53 ISSDD196 pKa = 3.08 RR197 pKa = 11.84 VHH199 pKa = 7.2 VIPCIPCGVCRR210 pKa = 11.84 RR211 pKa = 11.84 HH212 pKa = 6.55 HH213 pKa = 5.17 ITEE216 pKa = 4.48 IKK218 pKa = 10.37 CEE220 pKa = 4.08 EE221 pKa = 4.3 YY222 pKa = 11.22 VDD224 pKa = 3.7
Molecular weight: 25.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.368
IPC2_protein 7.673
IPC_protein 7.571
Toseland 7.161
ProMoST 8.068
Dawson 8.244
Bjellqvist 8.624
Wikipedia 8.17
Rodwell 8.258
Grimsley 7.161
Solomon 8.346
Lehninger 8.361
Nozaki 8.829
DTASelect 8.346
Thurlkill 8.39
EMBOSS 8.463
Sillero 8.726
Patrickios 4.342
IPC_peptide 8.346
IPC2_peptide 7.688
IPC2.peptide.svr19 7.749
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
224
224
224
224.0
25.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.571 ± 0.0
3.125 ± 0.0
5.804 ± 0.0
7.589 ± 0.0
4.911 ± 0.0
6.696 ± 0.0
2.232 ± 0.0
6.696 ± 0.0
6.25 ± 0.0
6.696 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.232 ± 0.0
3.571 ± 0.0
5.804 ± 0.0
2.679 ± 0.0
8.929 ± 0.0
6.696 ± 0.0
3.125 ± 0.0
7.589 ± 0.0
1.339 ± 0.0
4.464 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here