Mycobacterium phage Seagreen

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 105 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K2FPJ6|A0A0K2FPJ6_9CAUD Uncharacterized protein OS=Mycobacterium phage Seagreen OX=1698713 GN=SEAGREEN_75 PE=4 SV=1
MM1 pKa = 6.72NTSTNRR7 pKa = 11.84EE8 pKa = 4.05YY9 pKa = 10.46PSYY12 pKa = 11.1AGDD15 pKa = 3.75LALTLDD21 pKa = 4.0TSGDD25 pKa = 3.5TAKK28 pKa = 9.27ITVEE32 pKa = 4.13EE33 pKa = 4.15NGEE36 pKa = 4.43TVTVDD41 pKa = 4.14LDD43 pKa = 3.6AEE45 pKa = 4.33AARR48 pKa = 11.84EE49 pKa = 3.86LGTRR53 pKa = 11.84VIAYY57 pKa = 9.16NGSPSTWTIEE67 pKa = 3.8PMHH70 pKa = 7.81IGDD73 pKa = 4.21VEE75 pKa = 4.3LTVEE79 pKa = 4.58DD80 pKa = 4.17GQIILALPEE89 pKa = 4.2ATPFDD94 pKa = 4.92LGEE97 pKa = 4.2CDD99 pKa = 3.43IDD101 pKa = 4.22PFVHH105 pKa = 7.68DD106 pKa = 4.54FGQRR110 pKa = 11.84LIVWAGVDD118 pKa = 3.73LAVTADD124 pKa = 3.25AA125 pKa = 5.58

Molecular weight: 13.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K2FNB7|A0A0K2FNB7_9CAUD HNH endonuclease domain protein OS=Mycobacterium phage Seagreen OX=1698713 GN=SEAGREEN_64 PE=4 SV=1
MM1 pKa = 7.57RR2 pKa = 11.84RR3 pKa = 11.84GAKK6 pKa = 9.71LPEE9 pKa = 4.09WVVEE13 pKa = 4.49RR14 pKa = 11.84IVALSWNGWTTGDD27 pKa = 3.08IAKK30 pKa = 10.25EE31 pKa = 4.2VGCSDD36 pKa = 3.33RR37 pKa = 11.84TVSRR41 pKa = 11.84VRR43 pKa = 11.84VRR45 pKa = 11.84HH46 pKa = 5.55GISRR50 pKa = 11.84GEE52 pKa = 3.86AHH54 pKa = 7.28DD55 pKa = 5.3PIPEE59 pKa = 4.15HH60 pKa = 6.98VLAQAARR67 pKa = 11.84LLDD70 pKa = 4.61DD71 pKa = 4.18GASYY75 pKa = 10.87TDD77 pKa = 3.35AAATVGCSRR86 pKa = 11.84TALRR90 pKa = 11.84RR91 pKa = 11.84KK92 pKa = 9.78FPGRR96 pKa = 11.84GWDD99 pKa = 3.23RR100 pKa = 11.84RR101 pKa = 11.84QCAEE105 pKa = 3.42WRR107 pKa = 11.84VIARR111 pKa = 11.84MEE113 pKa = 3.81RR114 pKa = 11.84AIAA117 pKa = 3.76

Molecular weight: 13.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

105

0

105

18575

30

1174

176.9

19.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.719 ± 0.462

1.244 ± 0.176

6.67 ± 0.237

5.75 ± 0.276

2.816 ± 0.21

9.201 ± 0.638

2.186 ± 0.211

4.301 ± 0.175

3.37 ± 0.181

7.408 ± 0.233

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.197 ± 0.131

3.322 ± 0.158

6.078 ± 0.204

3.559 ± 0.199

6.95 ± 0.37

5.89 ± 0.272

6.396 ± 0.229

7.236 ± 0.274

2.25 ± 0.144

2.46 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski