Streptomyces sp. TLI_235

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8063 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A3HXG4|A0A2A3HXG4_9ACTN Cyclopropane-fatty-acyl-phospholipid synthase OS=Streptomyces sp. TLI_235 OX=1938860 GN=BX265_0741 PE=3 SV=1
MM1 pKa = 7.7ACRR4 pKa = 11.84GVHH7 pKa = 6.21FALTADD13 pKa = 3.36QSARR17 pKa = 11.84LLAAADD23 pKa = 3.99DD24 pKa = 4.37EE25 pKa = 4.75EE26 pKa = 4.52VLAVVEE32 pKa = 4.9EE33 pKa = 4.37IGEE36 pKa = 4.11AWDD39 pKa = 4.17CDD41 pKa = 3.76HH42 pKa = 7.54LAEE45 pKa = 5.33TDD47 pKa = 3.81KK48 pKa = 11.56AWDD51 pKa = 3.64ALHH54 pKa = 6.99RR55 pKa = 11.84CLADD59 pKa = 3.17GTLTYY64 pKa = 10.77EE65 pKa = 4.48GGDD68 pKa = 3.7YY69 pKa = 10.48PLSHH73 pKa = 6.84AVLGGLPLHH82 pKa = 7.33DD83 pKa = 4.78GDD85 pKa = 5.86DD86 pKa = 4.04YY87 pKa = 11.94VAVLTTPEE95 pKa = 4.01EE96 pKa = 4.28VADD99 pKa = 3.85IAAALAGLDD108 pKa = 3.85EE109 pKa = 4.38AWLRR113 pKa = 11.84EE114 pKa = 4.13RR115 pKa = 11.84FFALDD120 pKa = 3.38PEE122 pKa = 5.42DD123 pKa = 4.17YY124 pKa = 11.0DD125 pKa = 4.16GARR128 pKa = 11.84DD129 pKa = 3.83EE130 pKa = 6.26DD131 pKa = 3.97DD132 pKa = 3.6FAYY135 pKa = 9.03TRR137 pKa = 11.84YY138 pKa = 9.45WFGEE142 pKa = 3.69LCAFYY147 pKa = 10.43TRR149 pKa = 11.84AAADD153 pKa = 3.12GRR155 pKa = 11.84AAVFTVDD162 pKa = 3.15QQ163 pKa = 4.24

Molecular weight: 17.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A3I4V3|A0A2A3I4V3_9ACTN Non-specific serine/threonine protein kinase OS=Streptomyces sp. TLI_235 OX=1938860 GN=BX265_3437 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.77GRR40 pKa = 11.84TSLSAA45 pKa = 3.59

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8063

0

8063

2565824

29

8576

318.2

33.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.585 ± 0.044

0.817 ± 0.01

5.813 ± 0.024

5.344 ± 0.029

2.574 ± 0.016

9.62 ± 0.03

2.35 ± 0.016

2.918 ± 0.019

1.796 ± 0.021

10.541 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.574 ± 0.01

1.735 ± 0.018

6.562 ± 0.03

2.831 ± 0.018

8.205 ± 0.034

4.799 ± 0.023

6.186 ± 0.029

8.237 ± 0.026

1.545 ± 0.013

1.968 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski