Amino acid dipepetide frequency for Mycobacterium phage Scowl

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.608AlaAla: 13.608 ± 0.988
0.68AlaCys: 0.68 ± 0.19
6.371AlaAsp: 6.371 ± 0.622
6.371AlaGlu: 6.371 ± 0.751
3.155AlaPhe: 3.155 ± 0.434
7.856AlaGly: 7.856 ± 0.993
1.361AlaHis: 1.361 ± 0.392
4.515AlaIle: 4.515 ± 0.548
4.33AlaLys: 4.33 ± 0.527
9.526AlaLeu: 9.526 ± 0.917
2.474AlaMet: 2.474 ± 0.396
3.093AlaAsn: 3.093 ± 0.522
5.072AlaPro: 5.072 ± 0.701
3.031AlaGln: 3.031 ± 0.472
6.0AlaArg: 6.0 ± 0.685
4.886AlaSer: 4.886 ± 0.609
5.381AlaThr: 5.381 ± 0.556
8.783AlaVal: 8.783 ± 0.815
2.289AlaTrp: 2.289 ± 0.388
2.722AlaTyr: 2.722 ± 0.413
0.0AlaXaa: 0.0 ± 0.0
Cys
0.804CysAla: 0.804 ± 0.247
0.062CysCys: 0.062 ± 0.066
0.557CysAsp: 0.557 ± 0.211
0.866CysGlu: 0.866 ± 0.263
0.186CysPhe: 0.186 ± 0.095
0.866CysGly: 0.866 ± 0.288
0.186CysHis: 0.186 ± 0.126
0.124CysIle: 0.124 ± 0.094
0.309CysLys: 0.309 ± 0.139
0.495CysLeu: 0.495 ± 0.195
0.186CysMet: 0.186 ± 0.107
0.309CysAsn: 0.309 ± 0.146
0.309CysPro: 0.309 ± 0.133
0.186CysGln: 0.186 ± 0.119
0.371CysArg: 0.371 ± 0.132
0.68CysSer: 0.68 ± 0.234
0.309CysThr: 0.309 ± 0.147
0.309CysVal: 0.309 ± 0.14
0.186CysTrp: 0.186 ± 0.093
0.186CysTyr: 0.186 ± 0.14
0.0CysXaa: 0.0 ± 0.0
Asp
6.557AspAla: 6.557 ± 0.676
0.619AspCys: 0.619 ± 0.214
5.258AspAsp: 5.258 ± 0.622
3.959AspGlu: 3.959 ± 0.524
2.412AspPhe: 2.412 ± 0.381
6.0AspGly: 6.0 ± 0.709
1.299AspHis: 1.299 ± 0.288
2.722AspIle: 2.722 ± 0.41
2.907AspLys: 2.907 ± 0.543
7.113AspLeu: 7.113 ± 0.73
1.113AspMet: 1.113 ± 0.182
1.856AspAsn: 1.856 ± 0.314
4.701AspPro: 4.701 ± 0.593
1.361AspGln: 1.361 ± 0.338
4.021AspArg: 4.021 ± 0.504
3.093AspSer: 3.093 ± 0.514
3.773AspThr: 3.773 ± 0.409
4.825AspVal: 4.825 ± 0.562
1.732AspTrp: 1.732 ± 0.298
1.732AspTyr: 1.732 ± 0.318
0.0AspXaa: 0.0 ± 0.0
Glu
6.866GluAla: 6.866 ± 0.885
0.371GluCys: 0.371 ± 0.175
5.01GluAsp: 5.01 ± 0.478
5.258GluGlu: 5.258 ± 0.805
1.979GluPhe: 1.979 ± 0.431
4.454GluGly: 4.454 ± 0.525
1.299GluHis: 1.299 ± 0.294
3.402GluIle: 3.402 ± 0.476
2.289GluLys: 2.289 ± 0.408
6.618GluLeu: 6.618 ± 0.662
1.546GluMet: 1.546 ± 0.33
1.856GluAsn: 1.856 ± 0.418
2.35GluPro: 2.35 ± 0.456
2.722GluGln: 2.722 ± 0.432
3.711GluArg: 3.711 ± 0.602
3.464GluSer: 3.464 ± 0.358
3.897GluThr: 3.897 ± 0.563
5.196GluVal: 5.196 ± 0.528
1.423GluTrp: 1.423 ± 0.292
2.536GluTyr: 2.536 ± 0.496
0.0GluXaa: 0.0 ± 0.0
Phe
2.66PheAla: 2.66 ± 0.361
0.309PheCys: 0.309 ± 0.183
2.845PheAsp: 2.845 ± 0.381
2.041PheGlu: 2.041 ± 0.326
0.433PhePhe: 0.433 ± 0.149
3.216PheGly: 3.216 ± 0.472
0.68PheHis: 0.68 ± 0.258
1.546PheIle: 1.546 ± 0.299
1.485PheLys: 1.485 ± 0.328
2.722PheLeu: 2.722 ± 0.414
0.557PheMet: 0.557 ± 0.221
1.113PheAsn: 1.113 ± 0.246
1.608PhePro: 1.608 ± 0.309
1.175PheGln: 1.175 ± 0.248
1.67PheArg: 1.67 ± 0.318
1.794PheSer: 1.794 ± 0.283
1.979PheThr: 1.979 ± 0.351
2.35PheVal: 2.35 ± 0.334
0.557PheTrp: 0.557 ± 0.174
1.113PheTyr: 1.113 ± 0.297
0.0PheXaa: 0.0 ± 0.0
Gly
6.928GlyAla: 6.928 ± 0.978
0.804GlyCys: 0.804 ± 0.236
5.258GlyAsp: 5.258 ± 0.578
4.886GlyGlu: 4.886 ± 0.592
3.34GlyPhe: 3.34 ± 0.489
8.165GlyGly: 8.165 ± 1.821
1.67GlyHis: 1.67 ± 0.318
4.33GlyIle: 4.33 ± 0.643
3.526GlyLys: 3.526 ± 0.477
7.67GlyLeu: 7.67 ± 0.888
2.041GlyMet: 2.041 ± 0.414
3.526GlyAsn: 3.526 ± 0.541
3.897GlyPro: 3.897 ± 0.569
3.031GlyGln: 3.031 ± 0.397
4.206GlyArg: 4.206 ± 0.47
6.124GlySer: 6.124 ± 0.867
4.886GlyThr: 4.886 ± 0.73
5.381GlyVal: 5.381 ± 0.547
2.227GlyTrp: 2.227 ± 0.384
2.598GlyTyr: 2.598 ± 0.315
0.0GlyXaa: 0.0 ± 0.0
His
1.794HisAla: 1.794 ± 0.348
0.124HisCys: 0.124 ± 0.082
1.175HisAsp: 1.175 ± 0.255
1.423HisGlu: 1.423 ± 0.303
0.928HisPhe: 0.928 ± 0.207
1.485HisGly: 1.485 ± 0.369
0.557HisHis: 0.557 ± 0.169
0.928HisIle: 0.928 ± 0.216
0.99HisLys: 0.99 ± 0.306
0.928HisLeu: 0.928 ± 0.268
0.186HisMet: 0.186 ± 0.133
0.433HisAsn: 0.433 ± 0.155
1.361HisPro: 1.361 ± 0.281
1.052HisGln: 1.052 ± 0.285
1.299HisArg: 1.299 ± 0.304
0.495HisSer: 0.495 ± 0.165
0.99HisThr: 0.99 ± 0.246
1.423HisVal: 1.423 ± 0.297
0.433HisTrp: 0.433 ± 0.17
0.68HisTyr: 0.68 ± 0.235
0.0HisXaa: 0.0 ± 0.0
Ile
6.062IleAla: 6.062 ± 0.582
0.186IleCys: 0.186 ± 0.094
3.464IleAsp: 3.464 ± 0.424
3.959IleGlu: 3.959 ± 0.44
0.99IlePhe: 0.99 ± 0.26
3.897IleGly: 3.897 ± 0.454
0.866IleHis: 0.866 ± 0.227
1.67IleIle: 1.67 ± 0.334
1.732IleLys: 1.732 ± 0.343
3.155IleLeu: 3.155 ± 0.405
0.68IleMet: 0.68 ± 0.192
2.103IleAsn: 2.103 ± 0.373
3.216IlePro: 3.216 ± 0.397
1.299IleGln: 1.299 ± 0.279
3.773IleArg: 3.773 ± 0.578
3.402IleSer: 3.402 ± 0.55
3.34IleThr: 3.34 ± 0.445
2.969IleVal: 2.969 ± 0.516
0.68IleTrp: 0.68 ± 0.192
1.485IleTyr: 1.485 ± 0.253
0.0IleXaa: 0.0 ± 0.0
Lys
3.34LysAla: 3.34 ± 0.441
0.433LysCys: 0.433 ± 0.174
2.66LysAsp: 2.66 ± 0.442
2.289LysGlu: 2.289 ± 0.388
1.546LysPhe: 1.546 ± 0.305
2.412LysGly: 2.412 ± 0.361
1.237LysHis: 1.237 ± 0.275
2.783LysIle: 2.783 ± 0.569
2.103LysLys: 2.103 ± 0.433
3.402LysLeu: 3.402 ± 0.533
0.928LysMet: 0.928 ± 0.239
1.485LysAsn: 1.485 ± 0.249
2.722LysPro: 2.722 ± 0.391
1.67LysGln: 1.67 ± 0.38
3.216LysArg: 3.216 ± 0.496
2.598LysSer: 2.598 ± 0.399
2.35LysThr: 2.35 ± 0.391
3.526LysVal: 3.526 ± 0.624
0.742LysTrp: 0.742 ± 0.227
1.052LysTyr: 1.052 ± 0.294
0.0LysXaa: 0.0 ± 0.0
Leu
9.649LeuAla: 9.649 ± 0.977
0.309LeuCys: 0.309 ± 0.131
6.618LeuAsp: 6.618 ± 0.622
5.381LeuGlu: 5.381 ± 0.738
2.041LeuPhe: 2.041 ± 0.382
7.361LeuGly: 7.361 ± 0.793
1.546LeuHis: 1.546 ± 0.339
4.33LeuIle: 4.33 ± 0.519
4.082LeuLys: 4.082 ± 0.517
5.319LeuLeu: 5.319 ± 0.536
1.794LeuMet: 1.794 ± 0.315
2.722LeuAsn: 2.722 ± 0.447
6.0LeuPro: 6.0 ± 0.689
2.783LeuGln: 2.783 ± 0.5
6.185LeuArg: 6.185 ± 0.673
6.124LeuSer: 6.124 ± 0.629
5.876LeuThr: 5.876 ± 0.496
4.33LeuVal: 4.33 ± 0.648
0.928LeuTrp: 0.928 ± 0.328
2.289LeuTyr: 2.289 ± 0.356
0.0LeuXaa: 0.0 ± 0.0
Met
2.412MetAla: 2.412 ± 0.329
0.062MetCys: 0.062 ± 0.071
0.99MetAsp: 0.99 ± 0.262
1.237MetGlu: 1.237 ± 0.319
0.557MetPhe: 0.557 ± 0.153
1.299MetGly: 1.299 ± 0.268
0.247MetHis: 0.247 ± 0.115
0.928MetIle: 0.928 ± 0.282
1.052MetLys: 1.052 ± 0.218
1.237MetLeu: 1.237 ± 0.307
0.309MetMet: 0.309 ± 0.134
1.299MetAsn: 1.299 ± 0.246
0.928MetPro: 0.928 ± 0.242
0.557MetGln: 0.557 ± 0.178
1.361MetArg: 1.361 ± 0.322
1.856MetSer: 1.856 ± 0.346
2.598MetThr: 2.598 ± 0.358
1.113MetVal: 1.113 ± 0.301
0.247MetTrp: 0.247 ± 0.114
0.433MetTyr: 0.433 ± 0.167
0.0MetXaa: 0.0 ± 0.0
Asn
3.155AsnAla: 3.155 ± 0.51
0.247AsnCys: 0.247 ± 0.147
2.041AsnAsp: 2.041 ± 0.374
1.732AsnGlu: 1.732 ± 0.375
0.928AsnPhe: 0.928 ± 0.34
3.588AsnGly: 3.588 ± 0.578
0.742AsnHis: 0.742 ± 0.208
1.732AsnIle: 1.732 ± 0.323
0.866AsnLys: 0.866 ± 0.309
2.536AsnLeu: 2.536 ± 0.454
0.557AsnMet: 0.557 ± 0.158
0.804AsnAsn: 0.804 ± 0.222
2.845AsnPro: 2.845 ± 0.413
1.237AsnGln: 1.237 ± 0.232
1.423AsnArg: 1.423 ± 0.31
1.917AsnSer: 1.917 ± 0.432
1.856AsnThr: 1.856 ± 0.377
2.165AsnVal: 2.165 ± 0.367
0.928AsnTrp: 0.928 ± 0.218
1.175AsnTyr: 1.175 ± 0.292
0.0AsnXaa: 0.0 ± 0.0
Pro
5.938ProAla: 5.938 ± 0.607
0.433ProCys: 0.433 ± 0.198
4.144ProAsp: 4.144 ± 0.455
4.454ProGlu: 4.454 ± 0.454
2.227ProPhe: 2.227 ± 0.338
5.134ProGly: 5.134 ± 0.65
0.928ProHis: 0.928 ± 0.234
2.598ProIle: 2.598 ± 0.452
1.917ProLys: 1.917 ± 0.315
4.515ProLeu: 4.515 ± 0.529
0.99ProMet: 0.99 ± 0.241
1.608ProAsn: 1.608 ± 0.342
3.093ProPro: 3.093 ± 0.522
1.423ProGln: 1.423 ± 0.332
2.722ProArg: 2.722 ± 0.476
3.835ProSer: 3.835 ± 0.503
3.588ProThr: 3.588 ± 0.523
3.959ProVal: 3.959 ± 0.431
0.804ProTrp: 0.804 ± 0.283
1.546ProTyr: 1.546 ± 0.337
0.0ProXaa: 0.0 ± 0.0
Gln
3.588GlnAla: 3.588 ± 0.782
0.062GlnCys: 0.062 ± 0.064
1.237GlnAsp: 1.237 ± 0.336
1.608GlnGlu: 1.608 ± 0.263
1.237GlnPhe: 1.237 ± 0.259
2.907GlnGly: 2.907 ± 0.406
0.557GlnHis: 0.557 ± 0.172
2.289GlnIle: 2.289 ± 0.448
1.485GlnLys: 1.485 ± 0.409
3.897GlnLeu: 3.897 ± 0.508
0.742GlnMet: 0.742 ± 0.217
0.619GlnAsn: 0.619 ± 0.176
2.165GlnPro: 2.165 ± 0.389
1.917GlnGln: 1.917 ± 0.396
1.732GlnArg: 1.732 ± 0.383
1.608GlnSer: 1.608 ± 0.319
1.423GlnThr: 1.423 ± 0.289
2.722GlnVal: 2.722 ± 0.424
0.68GlnTrp: 0.68 ± 0.169
0.557GlnTyr: 0.557 ± 0.171
0.0GlnXaa: 0.0 ± 0.0
Arg
5.196ArgAla: 5.196 ± 0.656
0.928ArgCys: 0.928 ± 0.321
3.155ArgAsp: 3.155 ± 0.41
4.454ArgGlu: 4.454 ± 0.757
1.917ArgPhe: 1.917 ± 0.414
4.886ArgGly: 4.886 ± 0.669
0.866ArgHis: 0.866 ± 0.273
3.216ArgIle: 3.216 ± 0.454
2.845ArgLys: 2.845 ± 0.467
6.124ArgLeu: 6.124 ± 0.751
1.856ArgMet: 1.856 ± 0.37
2.289ArgAsn: 2.289 ± 0.489
2.412ArgPro: 2.412 ± 0.351
1.917ArgGln: 1.917 ± 0.333
5.01ArgArg: 5.01 ± 0.751
3.711ArgSer: 3.711 ± 0.496
2.845ArgThr: 2.845 ± 0.477
4.948ArgVal: 4.948 ± 0.563
1.175ArgTrp: 1.175 ± 0.336
1.732ArgTyr: 1.732 ± 0.326
0.0ArgXaa: 0.0 ± 0.0
Ser
6.618SerAla: 6.618 ± 0.635
0.495SerCys: 0.495 ± 0.187
3.649SerAsp: 3.649 ± 0.406
4.082SerGlu: 4.082 ± 0.511
1.979SerPhe: 1.979 ± 0.417
6.185SerGly: 6.185 ± 0.768
1.361SerHis: 1.361 ± 0.319
2.783SerIle: 2.783 ± 0.412
2.845SerLys: 2.845 ± 0.425
5.134SerLeu: 5.134 ± 0.672
1.485SerMet: 1.485 ± 0.362
1.794SerAsn: 1.794 ± 0.417
3.588SerPro: 3.588 ± 0.488
2.227SerGln: 2.227 ± 0.315
3.155SerArg: 3.155 ± 0.429
3.526SerSer: 3.526 ± 0.697
2.783SerThr: 2.783 ± 0.505
3.526SerVal: 3.526 ± 0.433
1.299SerTrp: 1.299 ± 0.288
1.175SerTyr: 1.175 ± 0.291
0.0SerXaa: 0.0 ± 0.0
Thr
5.814ThrAla: 5.814 ± 0.706
0.433ThrCys: 0.433 ± 0.148
4.454ThrAsp: 4.454 ± 0.48
4.33ThrGlu: 4.33 ± 0.542
2.227ThrPhe: 2.227 ± 0.376
5.814ThrGly: 5.814 ± 0.683
0.928ThrHis: 0.928 ± 0.221
2.536ThrIle: 2.536 ± 0.559
2.536ThrLys: 2.536 ± 0.357
5.258ThrLeu: 5.258 ± 0.672
0.99ThrMet: 0.99 ± 0.236
1.67ThrAsn: 1.67 ± 0.354
3.588ThrPro: 3.588 ± 0.521
1.485ThrGln: 1.485 ± 0.281
3.402ThrArg: 3.402 ± 0.564
3.34ThrSer: 3.34 ± 0.543
4.825ThrThr: 4.825 ± 0.579
5.629ThrVal: 5.629 ± 0.651
0.928ThrTrp: 0.928 ± 0.239
1.979ThrTyr: 1.979 ± 0.351
0.0ThrXaa: 0.0 ± 0.0
Val
6.557ValAla: 6.557 ± 0.662
0.495ValCys: 0.495 ± 0.194
5.258ValAsp: 5.258 ± 0.569
4.948ValGlu: 4.948 ± 0.614
2.289ValPhe: 2.289 ± 0.43
4.701ValGly: 4.701 ± 0.674
1.299ValHis: 1.299 ± 0.276
3.773ValIle: 3.773 ± 0.525
3.588ValLys: 3.588 ± 0.6
5.567ValLeu: 5.567 ± 0.54
1.237ValMet: 1.237 ± 0.324
2.289ValAsn: 2.289 ± 0.338
3.897ValPro: 3.897 ± 0.464
2.103ValGln: 2.103 ± 0.349
5.072ValArg: 5.072 ± 0.535
4.763ValSer: 4.763 ± 0.526
5.876ValThr: 5.876 ± 0.561
5.134ValVal: 5.134 ± 0.638
1.052ValTrp: 1.052 ± 0.284
2.35ValTyr: 2.35 ± 0.432
0.0ValXaa: 0.0 ± 0.0
Trp
1.67TrpAla: 1.67 ± 0.362
0.186TrpCys: 0.186 ± 0.104
1.423TrpAsp: 1.423 ± 0.271
0.928TrpGlu: 0.928 ± 0.224
0.928TrpPhe: 0.928 ± 0.24
1.67TrpGly: 1.67 ± 0.316
0.371TrpHis: 0.371 ± 0.155
1.052TrpIle: 1.052 ± 0.213
0.309TrpLys: 0.309 ± 0.213
1.856TrpLeu: 1.856 ± 0.323
0.371TrpMet: 0.371 ± 0.151
0.433TrpAsn: 0.433 ± 0.157
0.742TrpPro: 0.742 ± 0.261
0.866TrpGln: 0.866 ± 0.216
1.113TrpArg: 1.113 ± 0.294
0.99TrpSer: 0.99 ± 0.276
1.67TrpThr: 1.67 ± 0.425
1.917TrpVal: 1.917 ± 0.313
0.619TrpTrp: 0.619 ± 0.221
0.433TrpTyr: 0.433 ± 0.175
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.289TyrAla: 2.289 ± 0.42
0.247TyrCys: 0.247 ± 0.148
1.485TyrAsp: 1.485 ± 0.294
2.041TyrGlu: 2.041 ± 0.305
0.557TyrPhe: 0.557 ± 0.182
2.598TyrGly: 2.598 ± 0.455
0.68TyrHis: 0.68 ± 0.22
1.67TyrIle: 1.67 ± 0.337
1.299TyrLys: 1.299 ± 0.278
2.598TyrLeu: 2.598 ± 0.379
0.619TyrMet: 0.619 ± 0.163
1.175TyrAsn: 1.175 ± 0.302
1.361TyrPro: 1.361 ± 0.332
0.99TyrGln: 0.99 ± 0.236
2.103TyrArg: 2.103 ± 0.383
1.485TyrSer: 1.485 ± 0.291
1.917TyrThr: 1.917 ± 0.352
2.041TyrVal: 2.041 ± 0.31
0.619TyrTrp: 0.619 ± 0.23
0.619TyrTyr: 0.619 ± 0.209
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 91 proteins (16168 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski