Amino acid dipepetide frequency for Streptomyces sp. F001

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.894AlaAla: 19.894 ± 0.133	1.131AlaCys: 1.131 ± 0.021	8.186AlaAsp: 8.186 ± 0.066	8.831AlaGlu: 8.831 ± 0.086	3.431AlaPhe: 3.431 ± 0.039	12.107AlaGly: 12.107 ± 0.083	2.853AlaHis: 2.853 ± 0.038	3.576AlaIle: 3.576 ± 0.047	2.845AlaLys: 2.845 ± 0.044	14.019AlaLeu: 14.019 ± 0.091	2.48AlaMet: 2.48 ± 0.031	1.94AlaAsn: 1.94 ± 0.03	6.471AlaPro: 6.471 ± 0.061	3.983AlaGln: 3.983 ± 0.043	10.076AlaArg: 10.076 ± 0.075	5.774AlaSer: 5.774 ± 0.055	6.769AlaThr: 6.769 ± 0.063	12.011AlaVal: 12.011 ± 0.086	1.857AlaTrp: 1.857 ± 0.027	2.791AlaTyr: 2.791 ± 0.034	0.0AlaXaa: 0.0 ± 0.0
Cys	1.073CysAla: 1.073 ± 0.022	0.112CysCys: 0.112 ± 0.008	0.484CysAsp: 0.484 ± 0.016	0.446CysGlu: 0.446 ± 0.012	0.224CysPhe: 0.224 ± 0.01	1.018CysGly: 1.018 ± 0.021	0.214CysHis: 0.214 ± 0.009	0.205CysIle: 0.205 ± 0.009	0.117CysLys: 0.117 ± 0.007	0.768CysLeu: 0.768 ± 0.018	0.122CysMet: 0.122 ± 0.007	0.142CysAsn: 0.142 ± 0.007	0.524CysPro: 0.524 ± 0.015	0.183CysGln: 0.183 ± 0.008	0.66CysArg: 0.66 ± 0.017	0.469CysSer: 0.469 ± 0.014	0.566CysThr: 0.566 ± 0.019	0.672CysVal: 0.672 ± 0.017	0.146CysTrp: 0.146 ± 0.008	0.153CysTyr: 0.153 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.539AspAla: 7.539 ± 0.059	0.463AspCys: 0.463 ± 0.014	3.731AspAsp: 3.731 ± 0.047	4.017AspGlu: 4.017 ± 0.042	1.717AspPhe: 1.717 ± 0.03	6.305AspGly: 6.305 ± 0.066	1.454AspHis: 1.454 ± 0.025	2.032AspIle: 2.032 ± 0.029	1.266AspLys: 1.266 ± 0.027	6.231AspLeu: 6.231 ± 0.052	0.878AspMet: 0.878 ± 0.017	1.038AspAsn: 1.038 ± 0.023	4.388AspPro: 4.388 ± 0.046	1.611AspGln: 1.611 ± 0.026	4.984AspArg: 4.984 ± 0.045	2.588AspSer: 2.588 ± 0.034	3.308AspThr: 3.308 ± 0.044	4.889AspVal: 4.889 ± 0.048	1.066AspTrp: 1.066 ± 0.025	1.154AspTyr: 1.154 ± 0.024	0.0AspXaa: 0.0 ± 0.0
Glu	7.51GluAla: 7.51 ± 0.082	0.384GluCys: 0.384 ± 0.014	2.973GluAsp: 2.973 ± 0.042	3.752GluGlu: 3.752 ± 0.055	1.574GluPhe: 1.574 ± 0.023	4.392GluGly: 4.392 ± 0.042	1.578GluHis: 1.578 ± 0.028	2.372GluIle: 2.372 ± 0.034	1.555GluLys: 1.555 ± 0.026	7.095GluLeu: 7.095 ± 0.064	0.891GluMet: 0.891 ± 0.018	1.096GluAsn: 1.096 ± 0.021	3.465GluPro: 3.465 ± 0.041	2.467GluGln: 2.467 ± 0.034	5.539GluArg: 5.539 ± 0.052	2.63GluSer: 2.63 ± 0.034	2.965GluThr: 2.965 ± 0.037	4.562GluVal: 4.562 ± 0.038	0.818GluTrp: 0.818 ± 0.018	1.219GluTyr: 1.219 ± 0.024	0.0GluXaa: 0.0 ± 0.0
Phe	3.538PheAla: 3.538 ± 0.041	0.264PheCys: 0.264 ± 0.011	1.991PheAsp: 1.991 ± 0.031	1.514PheGlu: 1.514 ± 0.025	0.904PhePhe: 0.904 ± 0.022	2.942PheGly: 2.942 ± 0.037	0.625PheHis: 0.625 ± 0.016	0.724PheIle: 0.724 ± 0.018	0.523PheLys: 0.523 ± 0.018	2.643PheLeu: 2.643 ± 0.04	0.419PheMet: 0.419 ± 0.012	0.609PheAsn: 0.609 ± 0.016	1.405PhePro: 1.405 ± 0.024	0.733PheGln: 0.733 ± 0.017	1.916PheArg: 1.916 ± 0.029	1.415PheSer: 1.415 ± 0.026	2.048PheThr: 2.048 ± 0.031	2.282PheVal: 2.282 ± 0.037	0.433PheTrp: 0.433 ± 0.014	0.585PheTyr: 0.585 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	10.256GlyAla: 10.256 ± 0.081	0.877GlyCys: 0.877 ± 0.02	5.101GlyAsp: 5.101 ± 0.046	5.178GlyGlu: 5.178 ± 0.045	2.786GlyPhe: 2.786 ± 0.037	8.187GlyGly: 8.187 ± 0.075	2.255GlyHis: 2.255 ± 0.034	3.565GlyIle: 3.565 ± 0.045	2.426GlyLys: 2.426 ± 0.037	9.106GlyLeu: 9.106 ± 0.067	1.943GlyMet: 1.943 ± 0.031	1.831GlyAsn: 1.831 ± 0.033	4.841GlyPro: 4.841 ± 0.045	2.645GlyGln: 2.645 ± 0.036	7.718GlyArg: 7.718 ± 0.061	5.23GlySer: 5.23 ± 0.059	6.2GlyThr: 6.2 ± 0.064	7.336GlyVal: 7.336 ± 0.051	1.713GlyTrp: 1.713 ± 0.026	2.212GlyTyr: 2.212 ± 0.031	0.0GlyXaa: 0.0 ± 0.0
His	2.664HisAla: 2.664 ± 0.037	0.229HisCys: 0.229 ± 0.009	1.444HisAsp: 1.444 ± 0.025	1.283HisGlu: 1.283 ± 0.022	0.649HisPhe: 0.649 ± 0.015	2.377HisGly: 2.377 ± 0.034	0.735HisHis: 0.735 ± 0.021	0.754HisIle: 0.754 ± 0.019	0.375HisLys: 0.375 ± 0.012	2.479HisLeu: 2.479 ± 0.033	0.342HisMet: 0.342 ± 0.012	0.398HisAsn: 0.398 ± 0.013	1.813HisPro: 1.813 ± 0.029	0.692HisGln: 0.692 ± 0.017	2.188HisArg: 2.188 ± 0.033	1.04HisSer: 1.04 ± 0.022	1.409HisThr: 1.409 ± 0.026	1.704HisVal: 1.704 ± 0.029	0.394HisTrp: 0.394 ± 0.013	0.494HisTyr: 0.494 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	4.842IleAla: 4.842 ± 0.051	0.3IleCys: 0.3 ± 0.011	2.4IleAsp: 2.4 ± 0.031	2.131IleGlu: 2.131 ± 0.028	0.735IlePhe: 0.735 ± 0.02	3.646IleGly: 3.646 ± 0.045	0.666IleHis: 0.666 ± 0.018	0.947IleIle: 0.947 ± 0.023	0.765IleLys: 0.765 ± 0.02	2.563IleLeu: 2.563 ± 0.042	0.465IleMet: 0.465 ± 0.015	0.724IleAsn: 0.724 ± 0.016	1.898IlePro: 1.898 ± 0.028	0.78IleGln: 0.78 ± 0.021	2.356IleArg: 2.356 ± 0.031	1.707IleSer: 1.707 ± 0.027	2.357IleThr: 2.357 ± 0.034	2.851IleVal: 2.851 ± 0.039	0.414IleTrp: 0.414 ± 0.014	0.564IleTyr: 0.564 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	3.037LysAla: 3.037 ± 0.044	0.121LysCys: 0.121 ± 0.008	1.353LysAsp: 1.353 ± 0.026	1.312LysGlu: 1.312 ± 0.028	0.484LysPhe: 0.484 ± 0.014	1.905LysGly: 1.905 ± 0.027	0.451LysHis: 0.451 ± 0.013	0.893LysIle: 0.893 ± 0.021	0.91LysLys: 0.91 ± 0.026	2.105LysLeu: 2.105 ± 0.032	0.39LysMet: 0.39 ± 0.014	0.554LysAsn: 0.554 ± 0.018	1.392LysPro: 1.392 ± 0.027	0.758LysGln: 0.758 ± 0.018	1.517LysArg: 1.517 ± 0.025	1.203LysSer: 1.203 ± 0.024	1.322LysThr: 1.322 ± 0.028	1.999LysVal: 1.999 ± 0.031	0.302LysTrp: 0.302 ± 0.012	0.493LysTyr: 0.493 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	14.75LeuAla: 14.75 ± 0.104	0.873LeuCys: 0.873 ± 0.02	6.746LeuAsp: 6.746 ± 0.063	4.882LeuGlu: 4.882 ± 0.05	2.557LeuPhe: 2.557 ± 0.04	8.967LeuGly: 8.967 ± 0.065	2.37LeuHis: 2.37 ± 0.036	3.383LeuIle: 3.383 ± 0.05	2.175LeuLys: 2.175 ± 0.032	11.283LeuLeu: 11.283 ± 0.09	1.685LeuMet: 1.685 ± 0.027	1.754LeuAsn: 1.754 ± 0.027	6.427LeuPro: 6.427 ± 0.054	2.442LeuGln: 2.442 ± 0.036	8.674LeuArg: 8.674 ± 0.065	5.293LeuSer: 5.293 ± 0.05	6.882LeuThr: 6.882 ± 0.061	8.745LeuVal: 8.745 ± 0.066	1.292LeuTrp: 1.292 ± 0.026	1.917LeuTyr: 1.917 ± 0.032	0.0LeuXaa: 0.0 ± 0.0
Met	2.244MetAla: 2.244 ± 0.027	0.145MetCys: 0.145 ± 0.008	0.902MetAsp: 0.902 ± 0.019	0.778MetGlu: 0.778 ± 0.019	0.438MetPhe: 0.438 ± 0.015	1.323MetGly: 1.323 ± 0.024	0.371MetHis: 0.371 ± 0.011	0.687MetIle: 0.687 ± 0.02	0.448MetLys: 0.448 ± 0.015	1.672MetLeu: 1.672 ± 0.027	0.31MetMet: 0.31 ± 0.012	0.445MetAsn: 0.445 ± 0.013	1.121MetPro: 1.121 ± 0.023	0.474MetGln: 0.474 ± 0.015	1.432MetArg: 1.432 ± 0.026	1.32MetSer: 1.32 ± 0.021	1.586MetThr: 1.586 ± 0.023	1.291MetVal: 1.291 ± 0.025	0.225MetTrp: 0.225 ± 0.01	0.34MetTyr: 0.34 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.226AsnAla: 2.226 ± 0.031	0.187AsnCys: 0.187 ± 0.009	1.008AsnAsp: 1.008 ± 0.021	0.877AsnGlu: 0.877 ± 0.02	0.497AsnPhe: 0.497 ± 0.014	1.939AsnGly: 1.939 ± 0.038	0.434AsnHis: 0.434 ± 0.016	0.657AsnIle: 0.657 ± 0.017	0.463AsnLys: 0.463 ± 0.015	1.723AsnLeu: 1.723 ± 0.027	0.296AsnMet: 0.296 ± 0.013	0.463AsnAsn: 0.463 ± 0.016	1.382AsnPro: 1.382 ± 0.026	0.536AsnGln: 0.536 ± 0.017	1.345AsnArg: 1.345 ± 0.023	0.979AsnSer: 0.979 ± 0.02	1.136AsnThr: 1.136 ± 0.028	1.453AsnVal: 1.453 ± 0.026	0.327AsnTrp: 0.327 ± 0.012	0.433AsnTyr: 0.433 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	7.88ProAla: 7.88 ± 0.059	0.348ProCys: 0.348 ± 0.015	4.568ProAsp: 4.568 ± 0.052	4.423ProGlu: 4.423 ± 0.046	1.564ProPhe: 1.564 ± 0.027	6.158ProGly: 6.158 ± 0.064	1.501ProHis: 1.501 ± 0.024	1.339ProIle: 1.339 ± 0.028	1.247ProLys: 1.247 ± 0.024	5.293ProLeu: 5.293 ± 0.051	0.969ProMet: 0.969 ± 0.018	0.923ProAsn: 0.923 ± 0.018	3.377ProPro: 3.377 ± 0.049	1.813ProGln: 1.813 ± 0.03	3.855ProArg: 3.855 ± 0.04	3.195ProSer: 3.195 ± 0.044	3.272ProThr: 3.272 ± 0.044	5.42ProVal: 5.42 ± 0.048	0.912ProTrp: 0.912 ± 0.023	1.473ProTyr: 1.473 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	3.9GlnAla: 3.9 ± 0.042	0.19GlnCys: 0.19 ± 0.008	1.488GlnAsp: 1.488 ± 0.026	1.646GlnGlu: 1.646 ± 0.028	0.709GlnPhe: 0.709 ± 0.019	2.384GlnGly: 2.384 ± 0.033	0.713GlnHis: 0.713 ± 0.017	1.141GlnIle: 1.141 ± 0.02	0.658GlnLys: 0.658 ± 0.021	3.228GlnLeu: 3.228 ± 0.034	0.532GlnMet: 0.532 ± 0.016	0.541GlnAsn: 0.541 ± 0.015	1.75GlnPro: 1.75 ± 0.027	1.326GlnGln: 1.326 ± 0.03	2.554GlnArg: 2.554 ± 0.036	1.34GlnSer: 1.34 ± 0.025	1.459GlnThr: 1.459 ± 0.026	2.463GlnVal: 2.463 ± 0.034	0.508GlnTrp: 0.508 ± 0.015	0.662GlnTyr: 0.662 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	9.58ArgAla: 9.58 ± 0.088	0.677ArgCys: 0.677 ± 0.02	4.269ArgAsp: 4.269 ± 0.048	4.817ArgGlu: 4.817 ± 0.056	2.36ArgPhe: 2.36 ± 0.033	5.689ArgGly: 5.689 ± 0.052	2.168ArgHis: 2.168 ± 0.03	3.438ArgIle: 3.438 ± 0.038	1.786ArgLys: 1.786 ± 0.031	8.947ArgLeu: 8.947 ± 0.065	1.764ArgMet: 1.764 ± 0.029	1.388ArgAsn: 1.388 ± 0.025	5.013ArgPro: 5.013 ± 0.049	2.469ArgGln: 2.469 ± 0.033	7.916ArgArg: 7.916 ± 0.067	4.161ArgSer: 4.161 ± 0.043	5.489ArgThr: 5.489 ± 0.047	5.785ArgVal: 5.785 ± 0.049	1.427ArgTrp: 1.427 ± 0.025	1.857ArgTyr: 1.857 ± 0.026	0.0ArgXaa: 0.0 ± 0.0
Ser	6.579SerAla: 6.579 ± 0.053	0.425SerCys: 0.425 ± 0.015	2.782SerAsp: 2.782 ± 0.035	2.524SerGlu: 2.524 ± 0.034	1.536SerPhe: 1.536 ± 0.024	5.759SerGly: 5.759 ± 0.056	1.026SerHis: 1.026 ± 0.022	1.508SerIle: 1.508 ± 0.031	1.074SerLys: 1.074 ± 0.026	4.799SerLeu: 4.799 ± 0.05	1.096SerMet: 1.096 ± 0.02	0.907SerAsn: 0.907 ± 0.021	3.175SerPro: 3.175 ± 0.033	1.267SerGln: 1.267 ± 0.021	3.754SerArg: 3.754 ± 0.044	2.935SerSer: 2.935 ± 0.04	3.136SerThr: 3.136 ± 0.042	4.26SerVal: 4.26 ± 0.046	0.928SerTrp: 0.928 ± 0.019	1.299SerTyr: 1.299 ± 0.023	0.0SerXaa: 0.0 ± 0.0
Thr	8.734ThrAla: 8.734 ± 0.07	0.465ThrCys: 0.465 ± 0.016	3.729ThrAsp: 3.729 ± 0.039	3.451ThrGlu: 3.451 ± 0.035	1.68ThrPhe: 1.68 ± 0.03	6.51ThrGly: 6.51 ± 0.056	1.243ThrHis: 1.243 ± 0.023	1.766ThrIle: 1.766 ± 0.028	1.251ThrLys: 1.251 ± 0.026	5.744ThrLeu: 5.744 ± 0.052	0.978ThrMet: 0.978 ± 0.022	1.047ThrAsn: 1.047 ± 0.022	4.055ThrPro: 4.055 ± 0.044	1.434ThrGln: 1.434 ± 0.027	3.976ThrArg: 3.976 ± 0.045	3.211ThrSer: 3.211 ± 0.038	3.896ThrThr: 3.896 ± 0.049	6.228ThrVal: 6.228 ± 0.067	0.955ThrTrp: 0.955 ± 0.02	1.457ThrTyr: 1.457 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	10.336ValAla: 10.336 ± 0.068	0.757ValCys: 0.757 ± 0.018	5.013ValAsp: 5.013 ± 0.047	4.841ValGlu: 4.841 ± 0.046	2.46ValPhe: 2.46 ± 0.033	6.443ValGly: 6.443 ± 0.063	1.995ValHis: 1.995 ± 0.029	3.021ValIle: 3.021 ± 0.04	1.808ValLys: 1.808 ± 0.029	9.473ValLeu: 9.473 ± 0.081	1.432ValMet: 1.432 ± 0.027	1.678ValAsn: 1.678 ± 0.033	5.178ValPro: 5.178 ± 0.045	2.224ValGln: 2.224 ± 0.033	7.23ValArg: 7.23 ± 0.059	4.332ValSer: 4.332 ± 0.042	5.725ValThr: 5.725 ± 0.055	8.003ValVal: 8.003 ± 0.07	1.16ValTrp: 1.16 ± 0.023	1.599ValTyr: 1.599 ± 0.026	0.0ValXaa: 0.0 ± 0.0
Trp	1.658TrpAla: 1.658 ± 0.027	0.163TrpCys: 0.163 ± 0.008	0.855TrpAsp: 0.855 ± 0.022	0.749TrpGlu: 0.749 ± 0.016	0.53TrpPhe: 0.53 ± 0.016	1.064TrpGly: 1.064 ± 0.022	0.396TrpHis: 0.396 ± 0.014	0.577TrpIle: 0.577 ± 0.016	0.387TrpLys: 0.387 ± 0.012	1.794TrpLeu: 1.794 ± 0.029	0.301TrpMet: 0.301 ± 0.01	0.455TrpAsn: 0.455 ± 0.014	0.805TrpPro: 0.805 ± 0.019	0.685TrpGln: 0.685 ± 0.016	1.408TrpArg: 1.408 ± 0.027	0.96TrpSer: 0.96 ± 0.022	1.061TrpThr: 1.061 ± 0.022	1.01TrpVal: 1.01 ± 0.022	0.371TrpTrp: 0.371 ± 0.012	0.375TrpTyr: 0.375 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.782TyrAla: 2.782 ± 0.031	0.187TyrCys: 0.187 ± 0.008	1.635TyrAsp: 1.635 ± 0.032	1.386TyrGlu: 1.386 ± 0.026	0.677TyrPhe: 0.677 ± 0.018	2.289TyrGly: 2.289 ± 0.033	0.416TyrHis: 0.416 ± 0.013	0.528TyrIle: 0.528 ± 0.015	0.423TyrLys: 0.423 ± 0.012	2.134TyrLeu: 2.134 ± 0.033	0.257TyrMet: 0.257 ± 0.01	0.43TyrAsn: 0.43 ± 0.013	1.053TyrPro: 1.053 ± 0.023	0.671TyrGln: 0.671 ± 0.017	1.898TyrArg: 1.898 ± 0.028	0.985TyrSer: 0.985 ± 0.022	1.225TyrThr: 1.225 ± 0.028	1.741TyrVal: 1.741 ± 0.03	0.356TyrTrp: 0.356 ± 0.012	0.491TyrTyr: 0.491 ± 0.019	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7730 proteins (2477059 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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