Mycobacterium phage Guanica15

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TPL7|A0A5J6TPL7_9CAUD Uncharacterized protein OS=Mycobacterium phage Guanica15 OX=2599865 GN=80 PE=4 SV=1
MM1 pKa = 7.46SRR3 pKa = 11.84QHH5 pKa = 6.26YY6 pKa = 7.72CTGDD10 pKa = 3.83DD11 pKa = 3.38CWHH14 pKa = 6.5CEE16 pKa = 3.73RR17 pKa = 11.84RR18 pKa = 11.84ISQAEE23 pKa = 4.05YY24 pKa = 10.81EE25 pKa = 4.17RR26 pKa = 11.84DD27 pKa = 3.66CYY29 pKa = 11.44GDD31 pKa = 4.92DD32 pKa = 5.57DD33 pKa = 4.77YY34 pKa = 11.95PDD36 pKa = 4.53YY37 pKa = 11.71YY38 pKa = 11.27DD39 pKa = 3.5GTT41 pKa = 4.14

Molecular weight: 5.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TLR6|A0A5J6TLR6_9CAUD Uncharacterized protein OS=Mycobacterium phage Guanica15 OX=2599865 GN=91 PE=4 SV=1
MM1 pKa = 7.46TISTATRR8 pKa = 11.84SMTQMEE14 pKa = 4.44AHH16 pKa = 6.36QIAVGLIRR24 pKa = 11.84EE25 pKa = 4.45HH26 pKa = 7.58GLIGWTVSWDD36 pKa = 3.3NARR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84AGQCRR46 pKa = 11.84YY47 pKa = 9.56SSRR50 pKa = 11.84TISLSKK56 pKa = 10.14PLLRR60 pKa = 11.84QRR62 pKa = 11.84SYY64 pKa = 11.57DD65 pKa = 3.58DD66 pKa = 3.0TMMTITHH73 pKa = 7.35EE74 pKa = 4.05IAHH77 pKa = 6.47ALVGPKK83 pKa = 9.69HH84 pKa = 5.39GHH86 pKa = 5.05NAVWAAKK93 pKa = 9.29HH94 pKa = 5.49RR95 pKa = 11.84QLGGNGKK102 pKa = 9.49RR103 pKa = 11.84CFEE106 pKa = 4.77HH107 pKa = 8.56LDD109 pKa = 3.63EE110 pKa = 4.72SAPWMGTCSHH120 pKa = 6.25GKK122 pKa = 9.54KK123 pKa = 8.95FARR126 pKa = 11.84YY127 pKa = 7.72RR128 pKa = 11.84APKK131 pKa = 10.21RR132 pKa = 11.84LDD134 pKa = 2.84GWRR137 pKa = 11.84CKK139 pKa = 9.99CAAGSSPVVWVNQRR153 pKa = 2.94

Molecular weight: 17.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

102

0

102

19103

41

1375

187.3

20.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.239 ± 0.556

1.089 ± 0.133

6.533 ± 0.298

5.737 ± 0.308

2.654 ± 0.146

8.931 ± 0.497

2.22 ± 0.177

3.989 ± 0.168

3.204 ± 0.201

8.166 ± 0.215

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.24 ± 0.091

2.79 ± 0.194

5.591 ± 0.264

3.167 ± 0.151

7.208 ± 0.401

5.004 ± 0.245

5.722 ± 0.187

7.988 ± 0.282

2.193 ± 0.101

2.335 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski