Rotavirus X (strain RVX/Human/China/NADRV-J19/1997/GXP[X]) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-J19))
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q45UF4|NSP2_ROTJ1 Non-structural protein 2 OS=Rotavirus X (strain RVX/Human/China/NADRV-J19/1997/GXP[X]) OX=335103 PE=3 SV=1
MM1 pKa = 7.7 LLFIILSVGVDD12 pKa = 4.25 AISYY16 pKa = 8.33 IQNDD20 pKa = 3.93 RR21 pKa = 11.84 SNDD24 pKa = 3.24 LCIVYY29 pKa = 10.31 EE30 pKa = 4.1 MTSFGTSFNNANDD43 pKa = 4.0 SLVKK47 pKa = 10.02 LHH49 pKa = 6.26 KK50 pKa = 10.43 HH51 pKa = 5.35 MGLKK55 pKa = 10.53 YY56 pKa = 8.56 EE57 pKa = 4.06 VCKK60 pKa = 10.63 IEE62 pKa = 4.59 SNANALTQMQKK73 pKa = 10.65 CNCIYY78 pKa = 10.84 DD79 pKa = 3.9 DD80 pKa = 3.83 TPQIVVFTNFKK91 pKa = 10.35 KK92 pKa = 10.84 SSLKK96 pKa = 9.86 TLIGTEE102 pKa = 4.27 NKK104 pKa = 10.25 CEE106 pKa = 4.4 LLPQTTIYY114 pKa = 9.93 TPTVDD119 pKa = 3.67 IEE121 pKa = 4.43 SEE123 pKa = 4.23 YY124 pKa = 10.65 FIYY127 pKa = 10.84 GNDD130 pKa = 3.27 VKK132 pKa = 10.65 ICYY135 pKa = 9.55 LDD137 pKa = 3.8 KK138 pKa = 10.99 NLLGIGCDD146 pKa = 3.65 ATDD149 pKa = 3.49 TTSWLDD155 pKa = 3.57 LDD157 pKa = 5.21 AGLPTNHH164 pKa = 7.18 ALDD167 pKa = 3.8 IPEE170 pKa = 4.04 ITSDD174 pKa = 3.72 GFKK177 pKa = 10.96 LFAKK181 pKa = 10.44 YY182 pKa = 10.22 SDD184 pKa = 3.92 SFLCQRR190 pKa = 11.84 LMDD193 pKa = 4.14 EE194 pKa = 4.83 PKK196 pKa = 10.66 KK197 pKa = 10.58 QIQFYY202 pKa = 11.08 AEE204 pKa = 3.92 VDD206 pKa = 3.68 NVPSNDD212 pKa = 3.39 VIEE215 pKa = 4.87 SSRR218 pKa = 11.84 SWASVWKK225 pKa = 9.76 VVKK228 pKa = 9.48 TVLHH232 pKa = 5.47 FTYY235 pKa = 10.35 HH236 pKa = 6.84 ILDD239 pKa = 4.0 LFYY242 pKa = 11.15 GNRR245 pKa = 11.84 RR246 pKa = 11.84 ATARR250 pKa = 11.84 MIEE253 pKa = 4.08 HH254 pKa = 6.6 SPLGG258 pKa = 3.71
Molecular weight: 29.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.869
IPC2_protein 5.029
IPC_protein 4.965
Toseland 4.902
ProMoST 5.143
Dawson 4.991
Bjellqvist 5.118
Wikipedia 4.902
Rodwell 4.889
Grimsley 4.838
Solomon 4.991
Lehninger 4.952
Nozaki 5.118
DTASelect 5.334
Thurlkill 4.927
EMBOSS 4.952
Sillero 5.181
Patrickios 3.338
IPC_peptide 4.991
IPC2_peptide 5.169
IPC2.peptide.svr19 5.117
Protein with the highest isoelectric point:
>sp|Q5Y9A9|NSP3_ROTJ1 Non-structural protein 3 OS=Rotavirus X (strain RVX/Human/China/NADRV-J19/1997/GXP[X]) OX=335103 PE=3 SV=1
MM1 pKa = 7.46 EE2 pKa = 5.77 PEE4 pKa = 5.3 NYY6 pKa = 9.97 LAWLARR12 pKa = 11.84 DD13 pKa = 3.39 IVRR16 pKa = 11.84 NLSYY20 pKa = 10.97 TSLVYY25 pKa = 10.81 NNPKK29 pKa = 9.55 VAIVEE34 pKa = 4.04 LLDD37 pKa = 3.62 NKK39 pKa = 10.39 EE40 pKa = 3.94 AFFAYY45 pKa = 9.65 EE46 pKa = 4.35 KK47 pKa = 9.97 EE48 pKa = 4.32 QKK50 pKa = 9.36 TPEE53 pKa = 3.84 ALINYY58 pKa = 8.46 IDD60 pKa = 4.83 SIVKK64 pKa = 10.34 SSISVEE70 pKa = 4.21 DD71 pKa = 4.19 KK72 pKa = 11.0 IEE74 pKa = 4.01 ALLKK78 pKa = 9.63 IRR80 pKa = 11.84 YY81 pKa = 8.73 ISVYY85 pKa = 10.67 VDD87 pKa = 3.48 DD88 pKa = 5.96 KK89 pKa = 11.35 SDD91 pKa = 3.21 KK92 pKa = 10.6 RR93 pKa = 11.84 DD94 pKa = 3.24 IVLQLLNRR102 pKa = 11.84 TIKK105 pKa = 10.58 KK106 pKa = 9.8 IEE108 pKa = 4.34 LKK110 pKa = 10.11 TKK112 pKa = 10.49 ISDD115 pKa = 3.92 EE116 pKa = 4.19 LNDD119 pKa = 5.1 AINAITIEE127 pKa = 4.2 SKK129 pKa = 9.69 NWKK132 pKa = 8.2 IQNSKK137 pKa = 9.86 SFKK140 pKa = 9.22 PYY142 pKa = 10.11 HH143 pKa = 5.76 YY144 pKa = 9.69 NQLVSDD150 pKa = 4.36 FIKK153 pKa = 10.7 YY154 pKa = 10.5 NEE156 pKa = 4.03 FEE158 pKa = 4.39 VLEE161 pKa = 4.51 GTDD164 pKa = 3.57 PLKK167 pKa = 10.62 WKK169 pKa = 10.67 SDD171 pKa = 3.61 TLQGLSPNYY180 pKa = 9.03 NHH182 pKa = 6.95 RR183 pKa = 11.84 THH185 pKa = 6.5 TLISSIIYY193 pKa = 7.14 ATSVRR198 pKa = 11.84 FDD200 pKa = 3.58 NYY202 pKa = 10.75 NDD204 pKa = 3.49 EE205 pKa = 4.15 QLQVLLYY212 pKa = 9.04 LFSVIRR218 pKa = 11.84 TNYY221 pKa = 8.24 VNGYY225 pKa = 10.15 LEE227 pKa = 4.16 ILPNRR232 pKa = 11.84 KK233 pKa = 8.51 WSHH236 pKa = 5.57 SLADD240 pKa = 3.54 LRR242 pKa = 11.84 EE243 pKa = 4.27 NKK245 pKa = 10.34 SIMMYY250 pKa = 8.9 SAKK253 pKa = 9.91 IIHH256 pKa = 7.0 ASCAMISILHH266 pKa = 6.46 AVPIDD271 pKa = 3.7 YY272 pKa = 10.59 FFLAQIIASFSEE284 pKa = 4.52 IPAHH288 pKa = 5.87 AAKK291 pKa = 10.27 HH292 pKa = 5.53 LSSPMTLYY300 pKa = 10.33 IGIAQLRR307 pKa = 11.84 SNIVVSTKK315 pKa = 9.92 IAAEE319 pKa = 4.22 SVATEE324 pKa = 4.1 SPNISRR330 pKa = 11.84 LEE332 pKa = 3.79 EE333 pKa = 3.88 SQIRR337 pKa = 11.84 EE338 pKa = 4.16 WEE340 pKa = 4.18 QEE342 pKa = 3.59 MSEE345 pKa = 4.5 YY346 pKa = 10.3 PFQSSRR352 pKa = 11.84 MVRR355 pKa = 11.84 MMKK358 pKa = 10.45 KK359 pKa = 10.16 NIFDD363 pKa = 3.62 VSVDD367 pKa = 3.14 MFYY370 pKa = 10.93 AIFNCFSATFHH381 pKa = 5.9 VGHH384 pKa = 7.3 RR385 pKa = 11.84 IDD387 pKa = 4.2 NPQDD391 pKa = 3.85 AIEE394 pKa = 4.35 AQVKK398 pKa = 9.37 VEE400 pKa = 3.99 YY401 pKa = 10.21 TSDD404 pKa = 3.39 VDD406 pKa = 4.52 KK407 pKa = 11.43 EE408 pKa = 4.44 MYY410 pKa = 9.39 DD411 pKa = 3.01 QYY413 pKa = 11.88 YY414 pKa = 10.5 FLLKK418 pKa = 10.67 RR419 pKa = 11.84 MLTDD423 pKa = 3.02 QLAEE427 pKa = 4.24 YY428 pKa = 10.3 AEE430 pKa = 4.03 EE431 pKa = 4.9 MYY433 pKa = 10.57 FKK435 pKa = 11.09 YY436 pKa = 10.85 NSDD439 pKa = 3.22 VTAEE443 pKa = 4.22 SLAAMANSSNGYY455 pKa = 8.61 SRR457 pKa = 11.84 SVTFLDD463 pKa = 5.08 RR464 pKa = 11.84 EE465 pKa = 4.11 IKK467 pKa = 7.0 TTKK470 pKa = 10.6 KK471 pKa = 9.58 MLHH474 pKa = 6.67 LDD476 pKa = 3.84 DD477 pKa = 6.22 DD478 pKa = 4.59 LSKK481 pKa = 11.26 NLNFTNIGDD490 pKa = 4.04 QIKK493 pKa = 10.44 KK494 pKa = 9.16 GIPMGTRR501 pKa = 11.84 NVPARR506 pKa = 11.84 QTRR509 pKa = 11.84 GIFILSWQVAAIQHH523 pKa = 6.29 TIAEE527 pKa = 4.27 FLYY530 pKa = 10.6 KK531 pKa = 9.96 KK532 pKa = 10.11 AKK534 pKa = 9.99 KK535 pKa = 10.15 GGFGATFAEE544 pKa = 4.84 AYY546 pKa = 8.25 VAKK549 pKa = 10.32 AATLTYY555 pKa = 10.43 GILAEE560 pKa = 4.34 ATSKK564 pKa = 11.11 ADD566 pKa = 3.65 QLILYY571 pKa = 8.59 TDD573 pKa = 3.83 VSQWDD578 pKa = 3.7 ASQHH582 pKa = 4.09 NTEE585 pKa = 4.89 PYY587 pKa = 9.38 RR588 pKa = 11.84 SAWINAIKK596 pKa = 10.08 EE597 pKa = 3.85 ARR599 pKa = 11.84 TKK601 pKa = 11.14 YY602 pKa = 9.99 KK603 pKa = 10.28 INYY606 pKa = 7.3 NQEE609 pKa = 3.97 PVVLGMNVLDD619 pKa = 5.15 KK620 pKa = 10.67 MIEE623 pKa = 3.92 IQEE626 pKa = 4.22 ALLNSNLIVEE636 pKa = 4.62 SQGSKK641 pKa = 9.77 RR642 pKa = 11.84 QPLRR646 pKa = 11.84 IKK648 pKa = 9.73 YY649 pKa = 9.41 HH650 pKa = 5.48 GVASGEE656 pKa = 4.15 KK657 pKa = 6.55 TTKK660 pKa = 10.0 IGNSFANVALITTVFNNLTNTMPSIRR686 pKa = 11.84 VNHH689 pKa = 5.67 MRR691 pKa = 11.84 VDD693 pKa = 3.56 GDD695 pKa = 4.26 DD696 pKa = 3.95 NVVTMYY702 pKa = 8.46 TANRR706 pKa = 11.84 IDD708 pKa = 3.69 EE709 pKa = 4.2 VQEE712 pKa = 4.15 NIKK715 pKa = 10.47 EE716 pKa = 4.08 KK717 pKa = 9.82 YY718 pKa = 9.85 KK719 pKa = 11.03 RR720 pKa = 11.84 MNAKK724 pKa = 9.63 VKK726 pKa = 10.64 ALASYY731 pKa = 9.47 TGLEE735 pKa = 3.81 MAKK738 pKa = 10.23 RR739 pKa = 11.84 FIICGKK745 pKa = 8.84 IFEE748 pKa = 5.12 RR749 pKa = 11.84 GAISIFTAEE758 pKa = 4.28 RR759 pKa = 11.84 PYY761 pKa = 10.7 GTDD764 pKa = 3.38 LSVQSTTGSLIYY776 pKa = 10.34 SAAVNAYY783 pKa = 10.03 RR784 pKa = 11.84 GFGDD788 pKa = 3.23 NYY790 pKa = 10.3 LNFMTDD796 pKa = 2.95 VLVPPSASVKK806 pKa = 8.23 ITGRR810 pKa = 11.84 LRR812 pKa = 11.84 SLLSPVTLYY821 pKa = 9.93 STGPLSFEE829 pKa = 3.53 ITPYY833 pKa = 10.94 GLGGRR838 pKa = 11.84 MRR840 pKa = 11.84 LFSLSKK846 pKa = 11.03 EE847 pKa = 3.83 NMEE850 pKa = 4.64 LYY852 pKa = 10.53 KK853 pKa = 10.48 ILTSSLAISVQPDD866 pKa = 3.56 EE867 pKa = 4.24 IKK869 pKa = 10.5 KK870 pKa = 10.65 YY871 pKa = 10.93 SSTPQFKK878 pKa = 10.71 ARR880 pKa = 11.84 VDD882 pKa = 3.6 RR883 pKa = 11.84 MISSVQIAMKK893 pKa = 10.45 SEE895 pKa = 3.81 AKK897 pKa = 10.19 IITSILRR904 pKa = 11.84 DD905 pKa = 3.5 KK906 pKa = 11.2 EE907 pKa = 4.15 EE908 pKa = 3.97 QKK910 pKa = 9.79 TLGVPNVATTKK921 pKa = 10.23 NRR923 pKa = 11.84 QQIEE927 pKa = 4.36 KK928 pKa = 10.43 ARR930 pKa = 11.84 KK931 pKa = 6.86 TLSLPKK937 pKa = 9.23 EE938 pKa = 4.22 TLPKK942 pKa = 7.68 VTKK945 pKa = 10.04 YY946 pKa = 9.4 YY947 pKa = 10.06 PEE949 pKa = 4.86 EE950 pKa = 4.0 IFHH953 pKa = 7.04 LILRR957 pKa = 11.84 NSTFTIPKK965 pKa = 9.0 LNTMTKK971 pKa = 10.29 VYY973 pKa = 9.62 MNNSANITKK982 pKa = 9.83 LQQQLGVRR990 pKa = 11.84 VSSGIQVHH998 pKa = 6.02 RR999 pKa = 11.84 PVNTLLKK1006 pKa = 10.27 LVEE1009 pKa = 4.09 KK1010 pKa = 10.25 HH1011 pKa = 6.21 SPIKK1015 pKa = 10.33 ISPSDD1020 pKa = 3.49 LVLYY1024 pKa = 9.77 SKK1026 pKa = 10.84 KK1027 pKa = 10.5 YY1028 pKa = 10.93 DD1029 pKa = 3.52 LTNLNGKK1036 pKa = 8.57 KK1037 pKa = 9.66 QFLIDD1042 pKa = 4.27 LGISGNEE1049 pKa = 3.27 LRR1051 pKa = 11.84 FYY1053 pKa = 11.2 LNSKK1057 pKa = 10.15 LLFHH1061 pKa = 7.52 DD1062 pKa = 5.02 LLLSKK1067 pKa = 10.53 YY1068 pKa = 9.78 DD1069 pKa = 3.89 KK1070 pKa = 10.82 LYY1072 pKa = 10.32 EE1073 pKa = 4.32 SPGFGATQLNALPLDD1088 pKa = 3.78 LTAAEE1093 pKa = 4.59 KK1094 pKa = 10.98 VFSIKK1099 pKa = 10.75 LNLPNTYY1106 pKa = 10.33 YY1107 pKa = 10.77 EE1108 pKa = 4.47 LLMLILLYY1116 pKa = 10.51 EE1117 pKa = 4.11 YY1118 pKa = 11.42 VNFVMFTGDD1127 pKa = 3.21 TFRR1130 pKa = 11.84 AVCIPEE1136 pKa = 4.14 SQTINAKK1143 pKa = 9.63 LVKK1146 pKa = 9.55 TVMTMIDD1153 pKa = 4.35 NIQLDD1158 pKa = 4.0 TVMFSDD1164 pKa = 5.27 NIYY1167 pKa = 11.09
Molecular weight: 132.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.282
IPC2_protein 8.361
IPC_protein 8.214
Toseland 8.814
ProMoST 8.741
Dawson 9.165
Bjellqvist 8.931
Wikipedia 9.37
Rodwell 9.341
Grimsley 9.253
Solomon 9.194
Lehninger 9.151
Nozaki 8.916
DTASelect 8.902
Thurlkill 9.004
EMBOSS 9.282
Sillero 9.121
Patrickios 4.38
IPC_peptide 9.194
IPC2_peptide 7.468
IPC2.peptide.svr19 7.584
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11
0
11
5679
176
1167
516.3
58.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.758 ± 0.667
1.233 ± 0.379
6.093 ± 0.506
5.864 ± 0.41
4.543 ± 0.363
3.927 ± 0.414
1.849 ± 0.267
8.206 ± 0.271
6.92 ± 0.616
8.734 ± 0.444
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.8 ± 0.197
6.638 ± 0.384
3.645 ± 0.344
3.927 ± 0.292
5.001 ± 0.45
7.942 ± 0.406
6.286 ± 0.452
5.899 ± 0.41
0.775 ± 0.112
3.962 ± 0.466
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here